阅读说明：本技术 一种消炎杀菌的精油组合物、其制备方法及应用 (Anti-inflammatory and bactericidal essential oil composition, and preparation method and application thereof ) 是由 汪垠明 符春丽 田伟兰 祝荣荣 汪羿典 汪翔 卢正美 于 2019-12-04 设计创作，主要内容包括：本发明提供一种消炎杀菌的精油组合物,所述组合物通过茶树精油、薰衣草精油、艾叶精油、薄荷精油、迷迭香精油和百里香精油共同作用,协同达到提高消炎抗菌,延长挥发时间,提升精油持久性的功效。本发明提供的精油组合物一方面通过各组分中的酚类物质进入到微生物的细胞膜或细胞壁内,使细胞膜功能受到影响,促使细胞内容物外泄,破坏细胞结构,导致微生物的死亡；另一方面通过各组分中的萜类物质,对微生物代谢、还原型辅酶及其他酶的活性有抑制作用,导致微生物新陈代谢紊乱,达到抑菌的功效。本发明提供精油组合物通过各组分相互作用,协同增效,从而有效提高对细菌的抑制及杀灭效率,同时能够延长精油作用的时间,使得消炎抗菌效果持久。(The invention provides an anti-inflammatory and antibacterial essential oil composition which synergistically achieves the effects of improving anti-inflammatory and antibacterial effects, prolonging volatilization time and improving persistence of essential oil through combined action of tea tree essential oil, lavender essential oil, folium artemisiae argyi essential oil, mint essential oil, rosemary essential oil and thyme essential oil. On one hand, the essential oil composition provided by the invention has the advantages that phenolic substances in all components enter cell membranes or cell walls of microorganisms, so that the functions of the cell membranes are influenced, the contents of the cells are promoted to leak, the cell structures are damaged, and the microorganisms are killed; on the other hand, terpenoids in the components have the effect of inhibiting the metabolism of microorganisms, the activity of reduced coenzyme and other enzymes, so that the metabolism of the microorganisms is disordered, and the bacteriostatic effect is achieved. The essential oil composition provided by the invention has the advantages that the components interact with each other to realize synergistic interaction, so that the inhibition and killing efficiency on bacteria is effectively improved, the acting time of the essential oil can be prolonged, and the anti-inflammatory and antibacterial effects are durable.)

1. The anti-inflammatory and bactericidal essential oil composition is characterized by comprising the following components in parts by weight:

15-60 parts of tea tree essential oil;

15-60 parts of lavender essential oil;

15-60 parts of folium artemisiae argyi essential oil;

10-30 parts of mint essential oil;

5-35 parts of rosemary essential oil;

10-30 parts of thyme essential oil.

2. The anti-inflammatory and bactericidal essential oil composition as claimed in claim 1, which comprises the following components in parts by weight:

20 parts of tea tree essential oil;

20 parts of lavender essential oil;

20 parts of argy wormwood leaf essential oil;

15 parts of mint essential oil;

10 parts of rosemary essential oil;

15 parts of thyme essential oil.

3. The method for preparing an anti-inflammatory and anti-bacterial essential oil composition according to claim 1 or 2, which comprises the steps of:

(1) preparing tea tree essential oil: freeze drying, pulverizing, sieving to obtain tea tree powder, soaking in ethanol water solution, microwave extracting, and supercritical CO extracting₂Extracting, distilling and separating to obtain the tea tree essential oil;

(2) preparing lavender essential oil: taking lavender, freeze-drying, crushing and sieving to obtain lavender powder, then adding the lavender powder into an ethanol water solution, soaking, carrying out ultrasonic treatment, distilling and separating to obtain the lavender essential oil;

(3) preparing the folium artemisiae argyi essential oil: pulverizing folium Artemisiae Argyi, adding into distilled water, soaking, adding complex enzyme 0.5-0.8% of folium Artemisiae Argyi, performing enzymolysis, distilling, standing, and separating to obtain folium Artemisiae Argyi essential oil;

(4) preparing the mint essential oil: drying herba Menthae at 60-65 deg.C, pulverizing to obtain herba Menthae powder, adding herba Menthae powder into water, soaking, heating, collecting distillate, adding the distillate into anhydrous diethyl ether, extracting to obtain extractive solution, adding anhydrous sodium sulfate, and drying to obtain herba Menthae essential oil;

(5) preparing rosemary essential oil: taking rosemary, crushing, adding the rosemary into water for soaking, adjusting the pH value to 3-3.5, then adding cellulase accounting for 0.2-0.3% of the weight of the rosemary for enzymolysis to obtain enzymolysis liquid, and then distilling and separating to obtain rosemary essential oil;

(6) preparing thyme essential oil: pulverizing herba Thymi, sieving, adding into 90-95% ethanol solution, and supercritical CO₂Extracting at 35-40 deg.C under 20-25MPa with CO₂The flow rate of the thyme essential oil is 20-25L/min, a thyme crude extract is obtained after extraction, and then separation is carried out to obtain the thyme essential oil;

(7) uniformly mixing tea tree essential oil, lavender essential oil, folium artemisiae argyi essential oil, mint essential oil, rosemary essential oil and thyme essential oil to obtain the anti-inflammatory and bactericidal essential oil composition.

4. The method for preparing an anti-inflammatory and antibacterial essential oil composition according to claim 3, wherein the volume fraction of the ethanol aqueous solution in the step (1) is 70-80%.

5. The method for preparing an anti-inflammatory and antibacterial essential oil composition according to claim 3, wherein in the step (1), the supercritical CO is adopted₂The extraction temperature is 30-35 ℃, the extraction pressure is 20-25MPa, the fluid flow is 750-760L/h, and the extraction time is 3.5-4.5 h.

6. The method for preparing an anti-inflammatory and bactericidal essential oil composition as claimed in claim 3, wherein in the step (3), the complex enzyme is cellulase and pectinase, and the weight ratio of the cellulase to the pectinase is 2-3: 1.

7. The method for preparing an anti-inflammatory and antibacterial essential oil composition as claimed in claim 6, wherein in the step (3), the temperature of enzymolysis is 35-40 ℃, and the enzymolysis time is 25-35 min.

8. The method for preparing an anti-inflammatory and antibacterial essential oil composition as claimed in claim 6, wherein in the step (5), the temperature of enzymolysis is 25-35 ℃, and the time of enzymolysis is 1.5-2.5 h.

9. Use of an essential oil composition according to claim 1 or 2 in the manufacture of a medicament for the treatment of a disease in a woman.

10. Use according to claim 9, wherein the essential oil composition is present in the medicament at a concentration of 100 μ L/L or more.

Technical Field

The invention belongs to the technical field of essential oil, and particularly relates to an anti-inflammatory and bactericidal essential oil composition, and a preparation method and application thereof.

Background

Plant essential oil is also called essential oil or aromatic oil, and is a general name of volatile oily liquid with certain fragrance. Is a secondary metabolite produced in plants, and belongs to aliphatic, aromatic, terpenoid and sulfur-containing compounds. Plant essential oils may be present in different tissues of the plant. The plant essential oil serving as a novel antibacterial agent has multiple advantages, such as good antibacterial effect, small irritation to human bodies, small toxic and side effects and small environmental pollution. The research of the prior art shows that the bacteriostasis rate of the lavender essential oil directly contacting the alternaria alternata can reach 90.36 percent and 90.18 percent respectively; the clove essential oil has strong capability of inhibiting bacteria and the cinnamon essential oil has strong capability of inhibiting fungi, and the compounded essential oil has obvious bacteriostatic effect on escherichia coli and aspergillus niger; the bamboo leaf essential oil has an inhibiting effect on bacillus subtilis, staphylococcus aureus, escherichia coli and saccharomyces cerevisiae, and has the best inhibiting effect on the escherichia coli. However, most of the single-component essential oil researched in the prior art has the bacteriostatic effect, the single-component essential oil usually only has the inhibitory effect on single bacteria or certain bacteria, the bacteriostatic effect is effective, most of the single-component essential oil is easy to volatilize, the bacteriostatic aging is short, and the research on the anti-inflammatory and bactericidal effects of the compound essential oil is less.

Disclosure of Invention

In order to solve the technical problems, the invention provides an anti-inflammatory and bactericidal essential oil composition which synergistically achieves good anti-bacterial and anti-inflammatory effects through the combined action of a plurality of essential oils.

The invention aims to provide an anti-inflammatory and bactericidal essential oil composition.

The invention also aims to provide a preparation method of the anti-inflammatory and bactericidal essential oil composition.

The invention further aims to provide application of the anti-inflammatory and bactericidal essential oil composition.

According to the purpose of the invention, the anti-inflammatory and bactericidal essential oil composition provided by the invention comprises the following components in parts by weight:

15-60 parts of tea tree essential oil;

15-60 parts of lavender essential oil;

15-60 parts of folium artemisiae argyi essential oil;

10-30 parts of mint essential oil;

5-35 parts of rosemary essential oil;

10-30 parts of thyme essential oil.

The essential oil composition provided by the invention synergistically achieves the effects of improving the anti-inflammatory and antibacterial effects, prolonging the volatilization time, improving the persistence of the essential oil and improving the smell of single essential oil through the combined action of the tea tree essential oil, the lavender essential oil, the folium artemisiae argyi essential oil, the mint essential oil, the rosemary essential oil and the thyme essential oil. On one hand, the essential oil composition provided by the invention enters the cell membrane or cell wall of the microorganism through the phenolic substances, so that the function of the cell membrane is influenced, the content of the cell is promoted to leak, the cell structure is damaged, and the microorganism is killed; on the other hand, terpenoids have an inhibiting effect on the primary energy metabolism of microorganisms, the activity of reduced coenzyme and other enzymes, so that the metabolism of the microorganisms is disordered, and the bacteriostatic effect is achieved. The essential oil composition provided by the invention has the advantages that the components interact with each other to realize synergistic interaction, so that the inhibition and killing efficiency on bacteria is effectively improved, the acting time of the essential oil can be prolonged, and the anti-inflammatory and antibacterial effects are durable.

According to the invention, through the combined action of the tea tree essential oil, the lavender essential oil, the folium artemisiae argyi essential oil, the mint essential oil, the rosemary essential oil and the thyme essential oil, the components have certain sterilization effect when being used independently, but on one hand, the components have effective sterilization types and limited efficacy, and on the other hand, the components are easy to volatilize when being used independently, and the fragrance is single. According to the invention, the components are matched according to a specific weight ratio, so that the sterilization effect is synergistically enhanced, the action time can be prolonged, and the sterilization time effectiveness is improved. The invention has good bacteriostatic effect on escherichia coli, staphylococcus aureus and candida albicans.

In order to better explain the technical scheme of the invention, the efficacies of the components are provided below.

The tea tree essential oil mainly comprises ethylene (terpinene), terpineol (pinene), limonene (limonene), cineol (cineole), terpineol (terpineol), anise (cumene) and the like. The effective components in the tea tree essential oil can help the immune system to resist infectious diseases, and promote white blood cells to form a protective line, so that the tea tree essential oil is strong antibacterial essential oil. The tea tree essential oil has good curative effect on trichomonas, candida, mould and other fungal infections. However, the tea tree essential oil is volatile and can completely volatilize within 24 hours.

The lavender essential oil has the functions of clearing away heat and toxic materials, cleaning skin, controlling oil content, removing freckles, whitening skin, removing wrinkles, tendering skin, promoting regeneration of damaged tissues and the like. The lavender essential oil molecules stimulate olfactory nerves, so that the brain center is stimulated, the brain is excited, and nerve activity is regulated; through affinity action, the medicine enters into the subcutaneous part to improve the internal environment; enters the subcutaneous part through affinity action, promotes blood circulation and lymphatic circulation, and accelerates metabolism of the human body; the lavender essential oil can directly kill germs and microorganisms, and achieve the sterilization effect.

The main components of the folium Artemisiae Argyi essential oil are abrotanin, 4-hydroxy-8-acetoxyl-1, 12-lactone, absinthin, and esmoline. The wormwood essential oil has the main effects of regulating qi and blood, warming channels, dispelling cold and dampness, relieving pain, resisting bacteria, diminishing inflammation, exciting digestive glands, relieving cough and reducing phlegm, promoting circulation, tonifying qi and improving immunity.

The peppermint essential oil contains active substances such as menthol, eucalyptol, dibutyl phthalate, 1, 3-dioxane-5-ol, α -terpineol and the like, and has the effects of cleaning skin, calming and relieving, diminishing inflammation and easing pain, and soothing and refreshing.

The essential oil of rosemary contains terpenes such as monoterpene and sesquiterpene, has obvious effects of bacteriostasis, antioxidation, inflammation diminishing and pain relieving, and can promote blood circulation and stimulate hair regeneration; relieving skin congestion and edema; has the effects of resisting oxidation, delaying aging, reducing weight and blood fat, diminishing inflammation and sterilizing.

The thyme essential oil comprises main components of para-cymene, thymol, gamma-terpinene, eucalyptol, carvacrol methyl ether, β -bisabolene, borneol, hydrated sabinene and the like, can quickly permeate into pores and hair follicles from the skin, has the effects of strengthening nerves, activating cells, improving memory and attention and has strong bactericidal effect.

The anti-inflammatory and antibacterial essential oil composition preferably comprises the following components in parts by weight:

20 parts of tea tree essential oil;

20 parts of lavender essential oil;

20 parts of argy wormwood leaf essential oil;

15 parts of mint essential oil;

10 parts of rosemary essential oil;

15 parts of thyme essential oil.

The anti-inflammatory and antibacterial essential oil composition preferably comprises the following components in parts by weight:

15 parts of tea tree essential oil;

15 parts of lavender essential oil;

15 parts of argy wormwood leaf essential oil;

15 parts of mint essential oil;

15 parts of rosemary essential oil;

15 parts of thyme essential oil.

The invention provides a preparation method of the anti-inflammatory and bactericidal essential oil composition, which comprises the following steps:

(1) preparing tea tree essential oil: freeze drying, pulverizing, sieving to obtain tea tree powder, soaking in ethanol water solution, microwave extracting, and supercritical CO extracting₂Extracting, distilling and separating to obtain the tea tree essential oil;

(2) preparing lavender essential oil: taking lavender, freeze-drying, crushing and sieving to obtain lavender powder, then adding the lavender powder into an ethanol water solution, soaking, carrying out ultrasonic treatment, distilling and separating to obtain the lavender essential oil;

(3) preparing the folium artemisiae argyi essential oil: pulverizing folium Artemisiae Argyi, adding into distilled water, soaking, adding complex enzyme 0.5-0.8% of folium Artemisiae Argyi, performing enzymolysis, distilling, standing, and separating to obtain folium Artemisiae Argyi essential oil;

(4) preparing the mint essential oil: drying herba Menthae at 60-65 deg.C, pulverizing to obtain herba Menthae powder, adding herba Menthae powder into water, soaking, heating, collecting distillate, adding the distillate into anhydrous diethyl ether, extracting to obtain extractive solution, adding anhydrous sodium sulfate, and drying to obtain herba Menthae essential oil;

(5) preparing rosemary essential oil: taking rosemary, crushing, adding the rosemary into water for soaking, adjusting the pH value to 3-3.5, then adding cellulase accounting for 0.2-0.3% of the weight of the rosemary for enzymolysis to obtain enzymolysis liquid, and then distilling and separating to obtain rosemary essential oil;

(6) preparing thyme essential oil: pulverizing herba Thymi, sieving, adding into 90-95% ethanol solution, and supercritical CO₂Extracting at 35-40 deg.C under 20-25MPa with CO₂The flow rate of the thyme essential oil is 20-25L/min, a thyme crude extract is obtained after extraction, and then separation is carried out to obtain the thyme essential oil;

(7) uniformly mixing tea tree essential oil, lavender essential oil, folium artemisiae argyi essential oil, mint essential oil, rosemary essential oil and thyme essential oil to obtain the anti-inflammatory and bactericidal essential oil composition.

The preparation method of the essential oil composition provided by the invention is characterized in that effective substances in tea stems and leaves are reserved through freeze drying, and supercritical CO is assisted by microwave₂The extraction method is used for extracting effective substances in tea trees, so that the yield of the tea tree essential oil is ensured, and active substances in the tea tree essential oil are kept as far as possible; distilling off effective substances in lavender by means of freeze drying, ultrasonic treatment and distillation; by enzymatic hydrolysisThen, promoting the extraction of the folium artemisiae argyi essential oil in a steam distillation mode; extracting the mint essential oil by means of steam distillation and extraction, and ensuring that active substances are reserved in the obtained mint essential oil to the maximum extent; extracting rosemary essential oil by enzymolysis in combination with distillation, and performing supercritical CO extraction₂Extracting thyme essential oil by an extraction mode; and then uniformly mixing the essential oil components to obtain the essential oil composition. According to the preparation method of the essential oil composition, active substances in the essential oil are effectively reserved and the yield of the essential oil is improved in a reasonable mode according to the characteristics of the essential oil.

Preferably, in step (1), the volume fraction of the ethanol aqueous solution is 70-80%.

Preferably, in step (1), the supercritical CO₂The extraction temperature is 30-35 ℃, the extraction pressure is 20-25MPa, the fluid flow is 750-760L/h, and the extraction time is 3.5-4.5 h.

Preferably, in the step (3), the complex enzyme is cellulase and pectinase, and the weight ratio of the cellulase to the pectinase is 2-3: 1.

Preferably, in the step (3), the enzymolysis temperature is 35-40 ℃, and the enzymolysis time is 25-35 min.

Preferably, in the step (5), the enzymolysis temperature is 25-35 ℃, and the enzymolysis time is 1.5-2.5 h.

The invention provides enzymolysis conditions, and the mint and the rosemary are subjected to full enzymolysis according to the characteristics of the raw materials, so that full preparation is made for extracting the essential oil, and the yield of the essential oil is effectively improved.

The essential oil composition provided by the invention can be applied to the preparation of medicines for treating female diseases. Preferably, the concentration of the essential oil composition in the medicament is above 100 μ L/L. The essential oil composition provided by the invention has the effects of diminishing inflammation and resisting bacteria through the synergistic effect of the components, and has a good killing effect on pathogenic bacteria such as trichomonas which cause gynecological diseases.

The invention has the beneficial effects that:

1. the invention provides an anti-inflammatory and antibacterial essential oil composition which synergistically achieves the effects of improving anti-inflammatory and antibacterial effects, prolonging volatilization time, improving persistence of essential oil and improving smell of single essential oil through combined action of tea tree essential oil, lavender essential oil, folium artemisiae argyi essential oil, mint essential oil, rosemary essential oil and thyme essential oil. On one hand, the essential oil composition provided by the invention has the advantages that phenolic substances in all components enter cell membranes or cell walls of microorganisms, so that the functions of the cell membranes are influenced, the contents of cells are promoted to leak, the cell structures are damaged, and the microorganisms are killed; on the other hand, terpenoids in the components have an inhibiting effect on the primary energy metabolism of microorganisms, the activity of reduced coenzyme and other enzymes, so that the metabolism of the microorganisms is disordered, and the bacteriostatic effect is achieved. The essential oil composition provided by the invention has the advantages that the components interact with each other to realize synergistic interaction, so that the inhibition and killing efficiency on bacteria is effectively improved, the acting time of the essential oil can be prolonged, and the anti-inflammatory and antibacterial effects are durable.

2. According to the invention, the components are matched according to a specific weight ratio, so that the sterilization effect is synergistically enhanced, the action time can be prolonged, and the sterilization time effectiveness is improved. The invention has good bacteriostatic effect on escherichia coli, staphylococcus aureus and candida albicans.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

In the examples of the present invention, each part by weight is 1 g.