阅读说明：本技术 一种基于碳纳米管聚多巴胺复合材料微生物燃料电池的方法 (Method for preparing microbial fuel cell based on carbon nanotube polydopamine composite material ) 是由 陈可泉 冯娇 陆秋豪 黄书悦 许晟 王昕� 欧阳平凯 于 2019-11-28 设计创作，主要内容包括：本发明公开了一种基于碳纳米管聚多巴胺复合材料微生物燃料电池的方法,其特征在于,包括以下步骤：步骤1,将羟基化多臂碳纳米管加入溶剂超声分散；步骤2,将超声后的CNTs-COOH和盐酸多巴胺按质量比例1:0.6-:6混合得混合物；步骤3,将混合物接入搭建好的MFC阳极室中,使其HCl-DA的终浓度为0.1-1 mg/mL；步骤4,采用Ag/AgCl为参比电极,通过循环伏安法扫描在(-1.0)V-(+1.0)V进行电聚合。与现有技术相比,本发明微生物燃料电池通过碳纳米管聚多巴胺复合材料为原料,结合菌作为电池的阳极液,良好的稳定性和分散性,提高了MFC的产电量,具有良好的市场前景。(The invention discloses a method for preparing a microbial fuel cell based on a carbon nano tube polydopamine composite material, which is characterized by comprising the following steps of: step 1, adding a hydroxylated multi-arm carbon nanotube into a solvent for ultrasonic dispersion; step 2, mixing the CNTs-COOH subjected to ultrasonic treatment and dopamine hydrochloride according to the mass ratio of 1:0.6-6 to obtain a mixture; step 3, the mixture is connected into the built MFC anode chamber, so that the final concentration of HCl-DA is 0.1-1 mg/mL; and 4, adopting Ag/AgCl as a reference electrode, and scanning at (-1.0) V- (+1.0) V by cyclic voltammetry to perform electropolymerization. Compared with the prior art, the microbial fuel cell takes the carbon nano tube poly-dopamine composite material as the raw material and the binding bacteria as the anolyte of the cell, so that the microbial fuel cell has good stability and dispersibility, improves the electricity production of the MFC, and has good market prospect.)

1. A method for preparing a microbial fuel cell based on a carbon nanotube polydopamine composite material is characterized by comprising the following steps: step 1, adding CNTs-COOH into a solvent, and ultrasonically dispersing for 15-60 min; step 2, mixing the CNTs-COOH subjected to ultrasonic treatment and dopamine hydrochloride (HCl-DA) according to the mass ratio of 1:0.6-6 to obtain a mixture; step 3, the mixture is connected into the built MFC anode chamber, so that the final concentration of HCl-DA is 0.1-1 mg/mL; and 4, adopting Ag/AgCl as a reference electrode, and scanning at (-1.0) - (+1.0) V by cyclic voltammetry to perform electropolymerization.

2. The method for the microbial fuel cell based on the carbon nanotube poly dopamine composite material according to the claim 1, characterized in that the mass ratio of the C NTs-COOH and the dopamine hydrochloride (HCl-DA) in the step 2 is 1: 3.

3. The method for a carbon nanotube poly dopamine composite-based microbial fuel cell according to claim 1, characterized in that the final concentration of HCl-DA added in MFC in step 3 is 0.5 mg/ml.

4. The method for a microbial fuel cell based on carbon nanotube poly dopamine composite according to claim 1, characterized in that the sweep range of cyclic voltammetry in step 4 is (-0.8) V- (+0.6) V.

Technical Field

The invention belongs to the technical field of microbial fuel cell preparation, and particularly relates to a method for preparing a microbial fuel cell based on a carbon nano tube polydopamine composite material.

Background

Microbial Fuel Cells (MFCs) are devices that convert chemical energy into electrical energy using microorganisms as catalysts, and have attracted much attention due to their outstanding characteristics of degrading organic substances while simultaneously harvesting electrical energy. However, one of the major problems in the development of the method is that the output efficiency per unit electrode area is low, and the method cannot be applied to a good scale-up test in practical applications. The direct participation of the anode in the microbial catalytic oxidation process has been widely studied. Multi-arm Carbon Nanotubes (CNTs) are commonly used in MFCs to enhance the electrochemical performance of the cell because of their good electrical conductivity, large specific surface area, and increased cell contact area in MFCs.

CNTs are coaxial nanoscale tubular molecules formed by single-layer or multi-layer graphene sheets according to a certain helical angle, have highly delocalized large pi bonds, and have a plurality of non-bonded electrons in high-speed motion, so that the CNTs have good conductivity. But some drawbacks have been found in their application. (1) The CNTs are generally prepared into a solution, and the solution is dropped on the surface of an electrode material and is dried in air for use. The modification mode is very simple to operate and high in efficiency, but has poor stability, and usually falls off in a large area within 1-2 days of the operation of the battery. (2) CNTs have strong van der Waals force and large molecular weight, and are easy to agglomerate and wrap in aqueous solution and aggregate into large bundles or ropes. This limits their use and thus research on CNTs has primarily addressed the dispersion and stabilization problem. Ultrasound is a physical method commonly used for dispersing CNTs in a solution, which is simple to operate and takes effect quickly, but cannot maintain the dispersion state for a long time. (3) Exposure of CNTs can lead to cytotoxicity and genotoxicity, and also, in the case of microorganisms, the potential for damaging cell membranes.

Disclosure of Invention

Aiming at the technical problems, the invention provides a method for preparing a microbial fuel cell based on a carbon nano tube polydopamine composite material, the method is simple and easy to prepare, the stability and the dispersion are good, and the MFC of the obtained fuel cell has high electricity yield.

A method for preparing a microbial fuel cell based on a carbon nanotube polydopamine composite material comprises the following steps:

step 1, adding hydroxylated multi-arm carbon nanotube CNTs-COOH into a solvent, and ultrasonically dispersing for 15-60 min;

step 2, mixing the CNTs-COOH subjected to ultrasonic treatment and dopamine hydrochloride (HCl-DA) according to the mass ratio of 1:0.6-6 to obtain a mixture;

step 3, the mixture is connected into the built MFC anode chamber, so that the final concentration of HCl-DA is 0.1-1 mg/mL;

and 4, adopting Ag/AgCl as a reference electrode, and scanning at (-1.0) - (+1.0) V by cyclic voltammetry to perform electropolymerization.

As an improvement, the mass ratio of the C NTs-COOH to the dopamine hydrochloride (HCl-DA) in the step 2 is 1: 3.

As a modification, the final concentration of HCl-DA added to the MFC in step 3 was 0.5 mg/ml.

As a modification, the cyclic voltammetry scan in step 4 is performed under a three-electrode system, and the scan range is (-0.8) - (+0.6) V with Ag/AgCl as a reference electrode.

Has the advantages that:

compared with the prior art, the method for preparing the microbial fuel cell based on the carbon nano tube polydopamine composite material has the following advantages:

carboxylated Carbon Nanotubes (CNTs-COOH) with excellent chemical activity are combined with a dopamine material with good biocompatibility to be electrolyzed in an electrolytic cell of a proper electrolyte by a certain electrochemical mode, so that dopamine is polymerized on an electrode due to oxidation reduction, and the Carbon nanotube poly-dopamine composite material is formed. The CNTs can be improved to fall off by utilizing the adhesiveness of polydopamine, the damage of the CNTs to cells is reduced by good biocompatibility, and the soluble polymer polydopamine enables the carbon nanotubes to be adsorbed on the surface, so that the dispersion degree of the carbon nanotubes is improved. And simultaneously, electropolymerization is carried out in the MFC inoculated with the acting strain, so that the contact between the strain and the material can be effectively increased, and the strain can be more quickly adsorbed on the modified anode. Finally, the CNTs-COOH @ PDA composite material is utilized to have conductivity and strong adhesiveness, the dispersity of the CNTs-COOH is enhanced, and the CNTs-COOH acts on the anode of the microbial fuel cell, so that the electricity generation quantity of the MFC is improved.

Drawings

FIG. 1 is a diagram showing CNTs-COOH @ PDA suspension obtained after 0.5h, 1.0h, 1.5h, 2.0h, 2.5h and 3.0h of oxidative self-polymerization at pH 8.5, after standing for 10 min;

FIG. 2 is a scanning electron micrograph of carbon paste, carbon paste + CNTs-COOH and carbon paste + DA + CNTs-COOH after electropolymerization;

FIG. 3 shows the output voltages of the blank control group, the CNTs-COOH group and the DA + CNTs-COOH group, wherein a 2000 Ω resistor is externally connected between the MFC cathode and anode chambers, and the external voltage values are periodically measured by a multimeter.

Detailed Description

The invention is further described below with reference to the accompanying drawings and specific embodiments.