阅读说明：本技术 一种血液理化分析方法 (Blood physical and chemical analysis method ) 是由 杨力 马宇 徐志玲 王翔 蒋宇浩 于 2019-11-29 设计创作，主要内容包括：本发明涉及一种血液理化分析方法,属于血液分析领域。本发明首先将全血样本经过离心分离和分样装置分别形成血浆和血细胞,利用血液生化检测装置检测血浆得到全血样本的生化指标、利用血流变检测装置检测血浆得到全血样本血浆的粘度；然后将血浆与血细胞进行等量混合再次形成全血样品,通过血流变测量装置测量得到全血粘度。本发明的血液理化分析方法需要的样本量小、操作简单、测试时间段、测试过程可以减少存在的人为失误而导致样本位置放错问题,从而提高测试结果的准确性；另外本发明的分析方法还能同时水生化指标、血浆的粘度以及全血粘度的指标进行检测,具有良好的应用前景。(The invention relates to a blood physicochemical analysis method, and belongs to the field of blood analysis. The method comprises the steps of firstly, enabling a whole blood sample to pass through a centrifugal separation device and a sample separation device to respectively form plasma and blood cells, detecting the plasma by using a blood biochemical detection device to obtain biochemical indexes of the whole blood sample, and detecting the plasma by using a blood rheology detection device to obtain the viscosity of the plasma of the whole blood sample; then, the plasma and the blood cells are mixed in equal amounts to form a whole blood sample again, and the viscosity of the whole blood is measured by a blood rheology measuring device. The blood physicochemical analysis method has the advantages of small sample amount, simple operation, short test time and short test process, and can reduce the problem of misplacing the sample caused by human errors, thereby improving the accuracy of the test result; in addition, the analysis method can simultaneously detect the indexes of the water biochemical index, the blood plasma viscosity and the whole blood viscosity, and has good application prospect.)

1. A method for physicochemical analysis of blood, said method comprising the steps of:

(1) centrifuging a whole blood sample in an anticoagulation tube by using a centrifugal machine, and then placing the sample in a sample tube, wherein the upper layer in the sample tube is plasma and the lower layer is blood cells;

(2) passing the sample tube through a sample separation device to obtain plasma, transporting the plasma to a blood biochemical detection device, and measuring to obtain biochemical indexes of the whole blood sample;

(3) continuously distinguishing the sample tubes through a liquid level detection device, enabling the obtained upper-layer plasma to enter a blood rheology detection device, and measuring the viscosity of the whole blood sample plasma;

(4) and mixing the plasma in the blood rheology detection device and the blood cells in the sample tube in an equal amount in a blood mixing device to form a whole blood sample, and feeding the whole blood sample into a blood rheology measurement device to measure the viscosity of the whole blood.

2. A method for physicochemical analysis of blood according to claim 1, wherein the whole blood sample in step (1) is venous blood of a patient, and the volume of the anticoagulation tube is 5 ml.

3. A physicochemical analysis method for blood as defined in claim 1, wherein the rotation speed of said centrifuge in step (1) is 2000rpm, and the centrifugation is performed for 5-10 min.

4. A physicochemical analysis method for blood according to claim 1, wherein in step (3), the hemorheology detection device is a pressure-type hemorheology detection device.

5. A physicochemical analysis method for blood as set forth in claim 1, wherein the blood cells in step (4) are mixed by a stirring device before being mixed with the plasma.

6. A method for physicochemical analysis of blood according to claim 5, wherein the stirring means employs a paddle-type stirring blade combined with a sampling needle.

7. A blood physicochemical analysis method according to claim 1, wherein the blood blending device in step (4) is used for blending in a blowing and sucking manner.

Technical Field

The invention belongs to the field of blood analysis, and particularly relates to a blood physicochemical analysis method.

Background

The physical and chemical analyzer is a medical clinical chemical detecting instrument integrating electronic, optical, computer and various biochemical analyzers. It has all functions of full-automatic biochemical apparatus and blood rheometer, can analyze biochemical indexes of ALT (alanine aminotransferase), AST (aspartate aminotransferase), ALP (alkaline phosphatase), GGT (gamma-glutamine transferase), T-Bil (total bilirubin), D-Bill (direct bilirubin), GLU (glucose), CRE (creatinine), UA (uric acid) and the like of blood, and can analyze rheological indexes of blood such as: whole blood viscosity, plasma viscosity, hematocrit, erythrocyte sedimentation rate, sedimentation equation K value and the like. The blood biochemical detection and the pressure type blood rheology detection are two detection modes for different types of indexes of blood; the two detection modes are not combined together and are not applied to a complete medical instrument in the market. Because the sample that blood biochemical detection device needs is plasma, and the sample that pressure type blood rheological device needs is plasma and whole blood, consequently the connection between the detection device and reasonable sample distribution scheme can practice thrift the sample, reduce the sample pollution, can reduce test time again, improve detection efficiency. In the conventional medical detection, 1ml of plasma is usually required to be used as a sample for detecting the biochemical indexes of blood, and at least 2ml of venous blood of a patient needs to be collected according to the blood separation ratio; the detection of the blood rheology index requires the collection of 5ml of venous blood from the patient, and then approximately 7ml of whole blood would be required if we performed each type of index separately. And for the detection of the conventional hemorheology, whole blood is usually firstly sucked from a test tube, is conveyed to a hemorheology detection device through a sampling needle to detect the viscosity of the whole blood, is then discharged into an anticoagulation tube, is kept stand for 1-2h to obtain plasma or is centrifuged by a centrifuge to obtain the plasma, and the viscosity of the plasma is detected. The conventional detection method is required to detect two indexes, the required sample amount is large, the operation is complex, the testing time is long, and human errors possibly exist even when the test tube is drawn out for centrifugation in the testing process, so that the position of the sample is misplaced, and the accuracy of the testing result is influenced.

Therefore, a new blood physicochemical analysis method needs to be proposed.

Disclosure of Invention

In view of the above, the present invention provides a method for physicochemical analysis of blood.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for physicochemical analysis of blood, said method comprising the steps of:

(1) centrifuging a whole blood sample in an anticoagulation tube by using a centrifugal machine, and then placing the sample in a sample tube, wherein the upper layer in the sample tube is plasma and the lower layer is blood cells;

(2) passing the sample tube through a sample separation device to obtain plasma, transporting the plasma to a blood biochemical detection device, and measuring to obtain biochemical indexes of the whole blood sample;

(3) continuously distinguishing the sample tubes through a liquid level detection device, enabling the obtained upper-layer plasma to enter a blood rheology detection device, and measuring the viscosity of the plasma of the whole blood sample;

(4) and mixing the plasma in the blood rheology detection device and the blood cells in the sample tube in an equal amount in a blood mixing device to form a whole blood sample, entering the blood rheology measurement device, and measuring the whole blood viscosity of the whole blood sample.

Preferably, the whole blood sample in step (1) is venous blood of a patient, and the volume of the anticoagulation tube is 5 ml.

Preferably, the rotating speed of the centrifuge in the step (1) is 2000rpm, and the centrifugal treatment is carried out for 5-10 min.

Preferably, the hemorheology detecting device in the step (3) is a pressure-type hemorheology detecting device.

Preferably, the blood cells in step (4) need to be mixed by a stirring device before being mixed with the blood plasma.

Preferably, the stirring device adopts an inclined paddle type stirring sheet combined with the sampling needle.

Preferably, the blood blending device in the step (4) is used for blending in a blowing and sucking mode.

The invention has the beneficial effects that:

the invention discloses a blood physical and chemical analysis method, which comprises the steps of firstly, enabling a whole blood sample to pass through a centrifugal separation device and a sample separation device to respectively form plasma and blood cells, utilizing a blood biochemical detection device to detect the plasma to obtain biochemical indexes of the whole blood sample, and utilizing a blood rheology detection device to detect the plasma to obtain the viscosity of the plasma of the whole blood sample; then, the plasma and the blood cells are mixed in equal amounts to form a whole blood sample again, and the viscosity of the whole blood is measured by a blood rheology measuring device. The blood physical and chemical analysis method integrates the blood biochemical detection and the blood rheological detection, reduces the sample dosage, reduces the manual operation time on the premise of ensuring the accuracy of the test result, simplifies the complexity of multi-department measurement, can relieve the pain of the patient in blood sampling for many times, and improves the detection efficiency.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.

Detailed Description

The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.