阅读说明：本技术 一种通过调节肠道内环境来提高免疫力的仔猪复合预混剂及其制备方法和应用 (Piglet compound premix for improving immunity by regulating internal environment of intestinal tract and preparation method and application thereof ) 是由 朱小清 周盛昌 袁香丽 刘小成 张敬学 罗作明 吴有林 于 2019-11-25 设计创作，主要内容包括：本发明公开了一种通过调节肠道内环境来提高免疫力的仔猪复合预混剂及其制备方法和应用。该仔猪复合预混剂包括7～20份木醋液-乳杆菌混合液、5～18份表皮生长因子、6～30份毕赤酵母甘露寡糖、10～25份膳食纤维、6～16份淀粉和13～48份载体,该仔猪复合预混剂添加到仔猪饲料中能促进仔猪肠道发育,提高仔猪的免疫力,以促进仔猪生长,且安全无残留,不仅能缓解早期断奶导致的仔猪肠道问题,还能避免或减少抗生素的使用,降低养殖风险,提高养殖收益,更有利于畜牧业的可持续发展。(The invention discloses a piglet composite premix for improving immunity by regulating the internal environment of an intestinal tract, and a preparation method and application thereof. The piglet composite premix comprises 7-20 parts of pyroligneous liquor-lactobacillus mixed liquor, 5-18 parts of epidermal growth factors, 6-30 parts of pichia pastoris mannan oligosaccharide, 10-25 parts of dietary fibers, 6-16 parts of starch and 13-48 parts of carriers, and can promote piglet intestinal development and improve piglet immunity when being added into piglet feed, so that piglet growth is promoted, the piglet composite premix is safe and free of residues, the piglet intestinal problem caused by early weaning can be relieved, the use of antibiotics can be avoided or reduced, the breeding risk is reduced, the breeding income is improved, and the sustainable development of animal husbandry is facilitated.)

1. The piglet composite premix for improving the immunity by regulating the internal environment of the intestinal tract is characterized by comprising the following components in parts by mass: 7-20 parts of pyroligneous liquor-lactobacillus mixed liquor, 5-18 parts of epidermal growth factor, 6-30 parts of pichia pastoris mannan oligosaccharide, 10-25 parts of dietary fiber, 6-16 parts of starch and 13-48 parts of carrier.

2. The piglet composite premix according to claim 1, which comprises the following components in parts by mass: 10-14 parts of pyroligneous liquor-lactobacillus mixed liquor, 13-16 parts of epidermal growth factor, 15-20 parts of pichia pastoris mannan oligosaccharide, 18-22 parts of dietary fiber, 8-13 parts of starch and 13-34 parts of carrier.

3. The piglet composite premix according to claim 1, wherein the pyroligneous liquor-lactobacillus mixture is a pyroligneous liquor-lactobacillus plantarum mixture;

preferably, in the mixed liquid of wood vinegar and lactobacillus plantarum, the viable count of the mixed liquid of wood vinegar and lactobacillus plantarum is (1-9) × 10⁹CFU/mL, lactic acid concentration 20.18 ~ 20.54 g/L.

4. The piglet composite premix according to claim 3, wherein the pH value of the wood vinegar is 1.5-3.7;

preferably, in the wood vinegar, the organic acid accounts for 45-55% of the organic matter content; the organic acid is preferably acetic acid and/or butyric acid.

5. The piglet composite premix according to claim 1, wherein the dietary fiber is a soluble dietary fiber;

preferably, the carrier is defatted rice bran and/or rice hull powder.

6. The preparation method of the piglet composite premix according to any one of claims 1 to 5, which comprises the step of mixing the piglet composite premix according to parts by weight.

7. The application of the piglet composite premix as defined in any one of claims 1 to 5 in preparing piglet feed for improving immunity.

8. The use of the composite premix for piglets according to claim 7, for preparing a piglet feed for enhancing immunity, wherein the improvement of immunity is achieved by increasing one or more of total protein, albumin, immunoglobulin a, immunoglobulin G and immunoglobulin M in the serum of weaned piglets.

9. The use of the piglet composite premix according to claim 7 in the preparation of a piglet feed for enhancing immunity, wherein the piglet feed for enhancing immunity is characterized in that the index for enhancing the antioxidant function of the weaned piglet serum is obtained by enhancing the antioxidant function of the weaned piglet serum;

the serum antioxidant function indexes of the weaned piglets comprise: one or more of superoxide dismutase activity, glutathione peroxidase activity, and total antioxidant capacity.

10. The use of the piglet composite premix according to claim 9 in the preparation of feed for enhancing immunity, wherein the feed for enhancing immunity is prepared by enhancing the activity of superoxide dismutase to above 110.77U/mL;

preferably, the immunity is improved by improving the activity of glutathione peroxidase to be above 888.66U/mL;

preferably, the method for improving immunity is used for improving the total antioxidant capacity of weaned piglets to be more than 7.85.

Technical Field

The invention relates to the technical field of feeds, and particularly relates to a piglet composite premix for improving immunity by regulating the internal environment of intestinal tracts, and a preparation method and application thereof.

Background

The technology of early isolation weaning of piglets is widely applied to the contemporary pig raising industry, the early weaning of piglets can shorten the breeding cycle of sows, increase the annual delivery times of the sows and the utilization rate of a delivery stall, reduce the vertical spread of maternal diseases to the piglets, improve the health condition of the piglets and improve the growth performance of the piglets.

However, the digestive system of early weaned piglets is not developed to be mature, the immune system is imperfect, and in addition, the stress in the aspects of environment, nutrition, psychology and the like caused by weaning easily causes the problems of reduced feed intake, low immunity, intestinal mucosa injury, digestive dysfunction, intestinal microecological imbalance and the like of the weaned piglets, so that the digestive absorption rate of the piglets on nutrients is reduced, diarrhea is caused, the piglets grow slowly, even die, and great economic loss is caused to the pig industry.

The antibiotics with a certain proportion are added into the daily ration of the weaned pig, so that diarrhea can be prevented, and a certain growth promoting effect can be achieved. However, with the increasing awareness of food safety, environmental protection, and public health, problems such as increased bacterial resistance and antibiotic residues caused by long-term antibiotic abuse are receiving more and more attention.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a piglet composite premix for improving immunity by regulating the internal environment of intestinal tracts, a preparation method of the piglet composite premix and application of the piglet composite premix in preparing piglet feed for improving immunity.

The piglet composite premix can obviously improve the growth performance of weaned piglets, improve the intestinal health and reduce the diarrhea rate.

The invention is realized by the following steps:

in a first aspect, the embodiment of the invention provides a piglet composite premix for improving immunity by regulating the internal environment of intestinal tracts, which comprises the following components in parts by mass: 7-20 parts of pyroligneous liquor-lactobacillus mixed liquor, 5-18 parts of epidermal growth factor, 6-30 parts of pichia pastoris mannan oligosaccharide, 10-25 parts of dietary fiber, 6-16 parts of starch and 13-48 parts of carrier.

Specifically, the wood vinegar is an acidic liquid rich in organic acid and various mineral elements, contains water, organic matters, vitamins and trace mineral elements, is acidic, and can supplement gastric acid deficiency of young animals and promote proliferation of intestinal lactic acid bacteria.

Lactobacillus, a commonly used probiotic, ferments to produce organic acids, such as lactic acid and propionic acid, improves palatability of animal diets, lowers the pH in the gastrointestinal tract, and has bactericidal effects.

Epidermal Growth Factor (EGF), a single-chain polypeptide consisting of 53 amino acids, is secreted and released into the duodenal fluid, saliva, pancreatic fluid, blood and milk, mainly by submandibular gland, duodenal bulbar-lous (Brunner) gland and pancreas. EGF is a peptide substance which is found in breast milk and has the highest content and has an important regulation effect on the intestinal development of piglets, and can improve the intestinal morphological structure, the nutrient digestion and absorption and the growth performance of early weaned piglets by combining with intestinal mucosa receptors. The exogenous EGF is added into the feed, so that the diarrhea rate of weaned piglets can be reduced, the immunity and the growth performance are improved, and the intestinal development is promoted.

The pichia mannan oligosaccharide is a high molecular polymer extracted from cell walls of pichia pastoris, and can adjust the microbial flora structure of animal intestinal tracts, maintain the epithelial form of the intestinal tracts, regulate the immunity of organisms and the like; can improve local immunoreaction of organism by promoting the secretion of anti-inflammatory factor and regulating the gene expression of intestinal mucosa cell factor and improve the production performance of pig under the stress and disease state of organism.

The starch is added into the piglet composite premix, so that the adhesion among all the components can be promoted, the action effect among all the components can be improved, and the feed quality can be further improved through the adhesion.

All the components of the piglet composite premix are safe and harmless components, no drug resistance is generated, and the piglet composite premix has important effects on promoting intestinal development, regulating intestinal health and enhancing immunity, and can effectively reduce the diarrhea rate of weaned piglets and improve the growth performance of the piglets; and the components can be used in a synergistic and complementary manner, and the effect is obvious and stable.

In an optional embodiment, the piglet compound premix comprises the following components in parts by mass: 10-14 parts of pyroligneous liquor-lactobacillus mixed liquor, 13-16 parts of epidermal growth factor, 15-20 parts of pichia pastoris mannan oligosaccharide, 18-22 parts of dietary fiber, 8-13 parts of starch and 13-34 parts of carrier. The piglet feed premix obtained according to the proportion can further improve the immunity of piglets and reduce the diarrhea rate of the piglets.

In an alternative embodiment, the pyroligneous acid-lactobacillus mixture is a pyroligneous acid-lactobacillus plantarum mixture.

In an optional embodiment, the viable count in the mixed liquid of the wood vinegar and the lactobacillus plantarum is (1-9) × 10⁹CFU/mL, lactic acid concentration 20.18 ~ 20.54 g/L.

In an alternative embodiment, the viable count of the mixed liquid of pyroligneous liquor and lactobacillus plantarum is 4.57 × 10⁹CFU/mL, lactic acid concentration 20.36 g/L.

In an optional embodiment, the pH of the wood vinegar is 1.5 to 3.7;

in an alternative embodiment, the organic acid is 45% to 55% of the organic content of the wood vinegar. The organic acid is preferably acetic acid and/or butyric acid.

The compound premix containing the wood vinegar for the piglets is added into piglet feed, can supplement the gastric acid deficiency of young animals, keep the lower pH value in the stomach, and the lower pH value can promote the proliferation of intestinal lactobacillus and inhibit the growth of harmful bacteria with the optimum growth pH value of 6.0-8.0, such as escherichia coli, clostridium and staphylococcus.

The mixed liquid prepared from the wood vinegar and the lactobacillus plantarum not only plays the antioxidation function of the wood vinegar and the lactobacillus plantarum, but also plays the probiotic function of the wood vinegar, and further enhances the antioxidation capability of the lactobacillus plantarum.

In an alternative embodiment, the dietary fiber is a soluble dietary fiber. Although the soluble dietary fiber can not be degraded by enzymes in the digestive tract of a human body, the soluble dietary fiber can be fermented and degraded by microorganisms in the large intestine to generate short-chain fatty acids such as acetic acid, propionic acid and butyric acid, so that the pH value in the large intestine is reduced, the intestinal flora is changed, beneficial bacteria are increased, harmful bacteria are reduced, intestinal peristalsis is stimulated, the digestion and absorption of nutrients are improved, and the removal of metabolites is accelerated.

In an alternative embodiment, the carrier is defatted rice bran and/or rice hull flour.

In a second aspect, the present invention provides a method for preparing a piglet compound premix according to any one of the foregoing embodiments, which includes mixing the piglet compound premix according to the above parts by weight.

The preparation method is simple to operate and good in controllability, and the piglet composite premix capable of effectively reducing the diarrhea rate of weaned piglets, improving the immunity of the piglets and promoting the healthy growth of the piglets can be quickly prepared.

In a third aspect, the present invention provides a use of the piglet compound premix according to any one of the foregoing embodiments in preparing a piglet feed for improving immunity.

In an alternative embodiment, the above-mentioned method for enhancing immunity is by enhancing one or more of total protein, albumin, immunoglobulin a, immunoglobulin G and immunoglobulin M in serum of weaned piglets.

In an alternative embodiment, the above-mentioned method for improving immunity is an index for improving the antioxidant function of the serum of the weaned pig.

In an alternative embodiment, the serum antioxidant function index of the weaned pig comprises: one or more of superoxide dismutase activity, glutathione peroxidase activity, and total antioxidant capacity.

In an alternative embodiment, the enhancing immunity is achieved by increasing the activity of superoxide dismutase to greater than 110.77U/mL.

In an alternative embodiment, the enhancing immunity is achieved by increasing the activity of glutathione peroxidase to 888.66U/mL or greater.

In an alternative embodiment, the enhancing immunity is achieved by enhancing total antioxidant capacity of weaned pigs to above 7.85.

The invention has the following beneficial effects:

the embodiment of the invention provides a piglet composite premix for improving immunity by regulating the internal environment of intestinal tracts, which comprises a pyroligneous liquor-lactobacillus mixed liquor, an epidermal growth factor, pichia mannooligosaccharide, dietary fibers, starch and a carrier, can promote the intestinal tract development of piglets and improve the immunity of the piglets so as to promote the growth of the piglets, is safe and has no residue, can relieve the intestinal tract problem of the piglets caused by early weaning, can avoid or reduce the use of antibiotics, reduces the breeding risk, improves the breeding income and is more beneficial to the sustainable development of animal husbandry.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The features and properties of the present invention are described in further detail below with reference to examples.