阅读说明：本技术 厌氧酶解复合中药渣制备仔猪抗腹泻饲料的方法 (Method for preparing piglet anti-diarrhea feed by using anaerobic enzymolysis composite traditional Chinese medicine residues ) 是由 陆文清 曹云鹤 董冰 曹恒 乔鹏飞 于 2019-11-22 设计创作，主要内容包括：本发明公开了一种厌氧酶解复合中药渣制备仔猪抗腹泻饲料的方法,包括,以干姜药渣、陈皮药渣和苍术药渣为药物原料,与仔猪饲料原料混合后,添加红糖、纤维素酶和半纤维素酶,加水调节其含水率为30-50％,接种酵母菌和乳酸菌后混匀,在密闭环境下,酶解发酵即得。本发明的方法利用酵母菌和乳酸菌发酵仔猪饲料原料产生厌氧偏酸性的环境,促进纤维素酶和半纤维素酶酶解由干姜药渣、陈皮药渣和苍术药渣构成的药物原料,从而释放药物原料中的有效成分；操作简便,所需设备投资少,实际推广容易；制备得到的饲料安全,不含抗生素和激素等有害物质,能有效治疗仔猪腹泻,具有明显的经济效益和生态效益,推广价值良好,理论和实际意义重大。(The invention discloses a method for preparing piglet anti-diarrhea feed by anaerobic enzymolysis of composite traditional Chinese medicine residues, which comprises the steps of taking dried ginger residues, dried orange peel residues and rhizoma atractylodis residues as medicine raw materials, mixing the medicine raw materials with piglet feed raw materials, adding brown sugar, cellulase and hemicellulase, adding water to adjust the water content of the medicine raw materials to be 30-50%, inoculating saccharomycetes and lactic acid bacteria, mixing the mixture uniformly, and carrying out enzymolysis fermentation in a closed environment to obtain the piglet anti-diarrhea feed. According to the method, yeast and lactic acid bacteria are utilized to ferment the piglet feed raw materials to generate an anaerobic acidic environment, so that the medicinal raw materials consisting of rhizoma zingiberis dregs, pericarpium citri reticulatae dregs and rhizoma atractylodis dregs are promoted to be subjected to enzymolysis by cellulase and hemicellulase, and thus effective ingredients in the medicinal raw materials are released; the operation is simple, the investment of required equipment is low, and the practical popularization is easy; the prepared feed is safe, does not contain harmful substances such as antibiotics and hormones, can effectively treat the diarrhea of the piglets, has obvious economic and ecological benefits and good popularization value, and has great theoretical and practical significance.)

1. A method for preparing piglet anti-diarrhea feed by anaerobic enzymolysis of composite traditional Chinese medicine residues is characterized by comprising the steps of taking dried ginger residues, dried orange peel residues and rhizoma atractylodis residues as medicine raw materials, mixing the medicine raw materials with piglet feed raw materials, adding brown sugar, cellulase and hemicellulase, adding water to adjust the water content of the medicine raw materials to be 30-50%, inoculating saccharomycetes and lactic acid bacteria, mixing uniformly, and carrying out enzymolysis fermentation in a closed environment.

2. The method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues as claimed in claim 1, wherein the mass ratio of the medicine raw materials to the piglet feed raw materials is 1: 3-5, preferably 1: 4.

3. the method for preparing piglet anti-diarrhea feed by using the anaerobic enzymolysis composite traditional Chinese medicine residue according to claim 1 or 2, wherein in the medicine raw materials, the mass ratio of the dried ginger residue, the dried orange peel residue and the rhizoma atractylodis residue is (0.8-1.15): (1.88-2.15): (0.86-1.21), preferably (0.95-1.05): (1.92-2.10): (0.93-1.12).

4. The method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues as claimed in claim 3, is characterized in that the feed is prepared by adding the medicine raw materials and the feed raw materials,

the addition amount of the cellulase is 200-1000u/kg, preferably 400-600 u/kg;

and/or, the adding amount of the hemicellulase is 2000-10000u/kg, preferably 4000-6000 u/kg.

5. The method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues as claimed in claim 4, wherein the hemicellulase is xylanase.

6. The method for preparing piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues according to claim 1 or 2, wherein the ratio of the inoculation amounts of the yeast and the lactic acid bacteria is 1: (50-500);

preferably, the inoculation mode of the yeast and the lactic acid bacteria is to add a solid-state compound secondary strain containing the yeast and the lactic acid bacteria simultaneously, the inoculation amount of the solid-state compound secondary strain is 0.2 to 5.0 weight percent of the piglet feed raw material, and the content of the yeast and the content of the lactic acid bacteria in the solid-state compound secondary strain are respectively more than or equal to 1 x 10⁶cfu/g and 1X 10⁸cfu/g。

7. The method for preparing piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues as claimed in claim 6, wherein the lactic acid bacteria are a mixture of enterococcus faecium and streptococcus thermophilus, and are preferably (0.5-2.0): (0.5-2.0) a mixture of enterococcus faecium and Streptococcus thermophilus.

8. The method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residues according to any one of claims 1 to 7,

the piglet feed raw material is a mixture of wheat bran and soybean meal, and preferably comprises the following components in a mass ratio of 6-8: 1, a mixture of wheat bran and soybean meal;

and/or the temperature and the time of the enzymolysis fermentation are respectively 15-38 ℃ and 200-450 h.

9. The piglet anti-diarrhea feed prepared by the method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis compound traditional Chinese medicine residue as defined in any one of claims 1 to 8.

10. The use of the piglet anti-diarrhea feed according to claim 9 in the field of animal husbandry.

Technical Field

The invention relates to the field of feed products, in particular to a method for preparing piglet anti-diarrhea feed by anaerobic enzymolysis of composite traditional Chinese medicine residues.

Background

With the rapid development of social economy and the continuous improvement of the living standard of people, the food structure of residents is remarkably changed, the consumption of livestock products such as meat, milk, eggs and the like is continuously increased, and the livestock breeding is rapidly developed. The matched feed is also developed rapidly, and in addition, because the pork is the meat source with the largest edible amount in China and the piglet cultivation is an important part of the pig cultivation, the research and development of the piglet feed are more and more paid attention, and the development speed is rapid.

Chinese herbal medicines are important components of traditional medicine in China, and development of novel preparations and standardized production of Chinese patent medicines enable the Chinese herbal medicines to gradually move to the world; meanwhile, with the deepening of people's understanding of toxic and side effects of chemical drugs and the gradual formation of modern health concepts in recent years, Chinese medicine products with long history and unique curative effect are concerned with the characteristics of strong comprehensiveness, low toxicity and low residue, and the demand of the domestic and foreign medicine markets for Chinese medicines is increasing continuously. However, the Chinese medicine industry in China generates 3000 million tons of medicine residues every year, and according to the knowledge that most enterprises select a landfill or incineration method for treatment, the original and simple treatment mode not only brings great waste to the enterprises, but also particularly brings great hidden dangers to the environment and public health.

In the existing research and development of piglet feed, the research on recycling traditional Chinese medicine dregs to prepare piglet feed with specific functions is not many and has defects. Some feed production enterprises add the crushed dregs as additives into the feed, and some feed production enterprises use microorganisms to ferment the dregs, but the effect is poor.

So far, no report related to a method for preparing piglet anti-diarrhea feed by anaerobic enzymolysis of composite traditional Chinese medicine residues is seen.

Disclosure of Invention

Aiming at the defects in the prior art, the invention mainly aims to provide a method for preparing piglet anti-diarrhea feed by using anaerobic enzymolysis composite traditional Chinese medicine residues.

The invention also aims to provide the piglet anti-diarrhea feed prepared by the method.

The invention also aims to provide application of the piglet anti-diarrhea feed in the field of animal husbandry.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for preparing piglet anti-diarrhea feed by anaerobic enzymolysis of composite traditional Chinese medicine residue comprises mixing rhizoma Zingiberis residue, pericarpium Citri Tangerinae residue and rhizoma Atractylodis residue with piglet feed, adding brown sugar, cellulase and hemicellulase, adding water to adjust water content to 30-50%, inoculating yeast and lactobacillus, mixing, and performing enzymolysis fermentation under sealed environment.

Specifically, the piglet feed raw materials are fermented by the saccharomycetes and the lactic acid bacteria to generate an anaerobic slightly acidic environment, so that the medicinal raw materials consisting of dried ginger decoction dregs, dried orange peel decoction dregs and rhizoma atractylodis decoction dregs are promoted to be subjected to enzymolysis by the cellulase and the hemicellulase, and the effective ingredients in the medicinal raw materials are released.

In the technical scheme, the mass ratio of the medicine raw materials to the piglet feed raw materials is 1: 3-5.

Preferably, in the above technical scheme, the mass ratio of the drug raw material to the piglet feed raw material is 1: 4.

further, in the above technical scheme, in the above medicinal raw materials, the mass ratio of the dried ginger decoction dregs, the dried orange peel decoction dregs and the rhizoma atractylodis decoction dregs is (0.8-1.15): (1.88-2.15): (0.86-1.21).

Preferably, in the above technical scheme, in the medicinal raw materials, the mass ratio of the dried ginger decoction dregs, the dried orange peel decoction dregs and the rhizoma atractylodis decoction dregs is (0.95-1.05): (1.92-2.10): (0.93-1.12).

Specifically, in the technical scheme, the adding amount of the brown sugar is 6.5-8.8 wt% of the medicinal raw materials.

Still further, in the above technical solution, the adding amount of the cellulase is 200-1000u/kg, preferably 400-600u/kg, based on the sum of the adding amounts of the drug raw material and the feed raw material.

Still further, in the above technical solution, the adding amount of the hemicellulase is 2000-10000u/kg, preferably 4000-6000u/kg, based on the sum of the adding amounts of the drug raw material and the feed raw material.

In detail, in the above technical solution, the activity of the cellulase is CMCase activity unit, and the activity of the hemicellulase is oat xylanase activity unit.

Still further, in the above technical solution, the hemicellulase is xylanase.

Further, in the above technical solution, the ratio of the inoculation amounts of the yeast and the lactic acid bacteria is 1: (50-500).

Preferably, in the technical scheme, the inoculation mode of the yeast and the lactic acid bacteria is to add a solid-state composite secondary strain containing the yeast and the lactic acid bacteria simultaneously, the inoculation amount of the solid-state composite secondary strain is 0.2 to 5.0wt percent of the piglet feed raw material, and the content of the yeast and the content of the lactic acid bacteria in the solid-state composite secondary strain are respectively more than or equal to 1 x 10⁶cfu/g and 1X 10⁸cfu/g。

Still further, in the above technical solution, the lactic acid bacteria is a mixture of enterococcus faecium and streptococcus thermophilus.

Preferably, in the above technical solution, the lactic acid bacteria are (0.5-2.0): (0.5-2.0) a mixture of enterococcus faecium and Streptococcus thermophilus.

Specifically, in the technical scheme, the piglet feed raw material is a mixture of wheat bran and soybean meal.

Preferably, in the technical scheme, the piglet feed comprises the following raw materials in a mass ratio of 6-8: 1 of wheat bran and soybean meal.

Specifically, in the technical scheme, the temperature and the time of the enzymolysis fermentation are respectively 15-38 ℃ and 200-450 h.

The invention provides a piglet anti-diarrhea feed prepared by the method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis composite traditional Chinese medicine residues.

The invention also provides application of the piglet anti-diarrhea feed in the field of animal husbandry.

The invention has the advantages that:

(1) according to the method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis composite traditional Chinese medicine residues, the piglet feed raw materials are fermented by using saccharomycetes and lactic acid bacteria to generate an anaerobic slightly acidic environment, so that the medicine raw materials consisting of rhizoma zingiberis decoction residues, pericarpium citri reticulatae decoction residues and rhizoma atractylodis decoction residues are promoted to be subjected to enzymolysis by using cellulase and hemicellulase, and thus effective components in the medicine raw materials are released;

(2) the method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis composite traditional Chinese medicine residues is simple and convenient to operate, requires less equipment investment, and is easy to popularize practically;

(3) the feed prepared by the method for preparing the piglet anti-diarrhea feed by using the anaerobic enzymolysis composite traditional Chinese medicine residues is safe, does not contain harmful substances such as antibiotics and hormones, can effectively treat piglet diarrhea, has obvious economic and ecological benefits, is good in popularization value, and has great theoretical and practical significance.

Detailed Description

The following describes the embodiments of the present invention in further detail with reference to specific examples.

The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.

Unless otherwise defined, the technical means used in the embodiments of the present invention are conventional means well known to those skilled in the art.

All starting materials are commercially available if not otherwise specified.

1. Source and treatment of raw materials of the medicine:

respectively taking waste dried ginger residue, dried tangerine peel residue and rhizoma atractylodis residue from a traditional Chinese medicine preparation factory, and sequentially performing air flow drying, crushing and 40-mesh sieve to obtain dried ginger residue, dried tangerine peel residue and rhizoma atractylodis residue for later use.

2. Preparing a solid microbial inoculum:

2.1, raw materials:

brewing yeast powder (Angel yeast powder, viable count is not less than 100 hundred million cfu/g);

enterococcus faecium powder (Taiwan Suzhou biological technology limited company, viable count is not less than 1000 hundred million cfu/g);

streptococcus thermophilus (Taiwan Suzhou Biotech Co., Ltd., viable count is not less than 1000 hundred million cfu/g);

brown sugar (food grade);

milk (no antibiotic contamination).

2.2 appliances

A heating furnace (e.g., an electric rice cooker);

a stainless steel pan (about 5 liters);

a thermometer;

weigh the balance (precision to 0.01 gram);

a stirring rod (e.g., clean bamboo chopsticks);

a stainless steel cup;

glass triangular bottles (500 ml gauge);

medical gauze;

a thermostat;

small package fermentation bag (5kg package);

a heat seal sealing machine;

bean pulp (crushed through 40 mesh);

testa Tritici (processed at 80 deg.C for about 30 min, cooled to room temperature and reused).

2.3 Primary culture of enterococcus faecium

Preparing 1kg of milk without antibiotic pollution, pouring the milk into a stainless steel pot, adding 1kg of clear water (without any drug pollution), diluting, adding 70g of brown sugar, gradually heating and stirring, heating to about 90 ℃ after the brown sugar is dissolved, and keeping for about 20 minutes (the temperature is increased to over 95 ℃, and the milk is easy to overflow); then, the heating was stopped, the mixture was cooled to 40 ℃ or lower, 5g of enterococcus faecium powder was added, and the mixture was allowed to stand for 3 minutes and then stirred for 3 seconds.

The inoculated culture solution is subpackaged into triangular glass bottles with the volume of 500 ml, and each triangular glass bottle contains about 250 ml.

Note that: the flask was previously washed with hot water and dried.

The mouth of the bottle is sealed by 16 layers of medical gauze (the medical gauze is folded into 16 layers of square blocks), the square blocks are placed in a thermostat, and the square blocks are placed in the thermostat and can be used for next step of expanded culture after being statically cultured for 16 hours at 35 ℃.

2.4 Primary culture of Streptococcus thermophilus

The culture process is completely referred to the first-stage culture of enterococcus faecium.

5g of enterococcus faecium powder was replaced by 5g of Streptococcus thermophilus powder.

2.5 preparation of Yeast liquid

Preparing 2kg of clear water, adding 200g of brown sugar, gradually heating and stirring; stopping heating after the brown sugar is completely dissolved; cooling to about 30 deg.C, and adding 100g active dried yeast powder; stirring for about 3 minutes, and standing for about 20 minutes to be used for the next inoculation culture.

2.6 preparation of raw Material for second-class Strain

100kg of soybean meal (crushed through 40 meshes);

400kg of testa Tritici (without pulverizing, and sterilized at 80 deg.C to eliminate activity of mold spore);

250kg of brown sugar liquid (containing 10kg of brown sugar);

150 small fermentation bags (5kg packed);

75 plastic woven bags (each containing 2 small fermentation bags).

2.7 preparation of second-class strains

100kg of soybean meal, 400kg of wheat bran and 250kg of brown sugar solution are gradually added into a mixer.

After stirring and mixing for 3 minutes, 500 ml of enterococcus faecium primary culture solution, 500 ml of streptococcus thermophilus primary culture solution and 2kg of yeast activating solution are added.

Uniformly mixing for 1 minute, subpackaging in small breathing bags packaged by 5kg, sealing by heat seal, packaging in a plastic woven bag by every 2 small fermentation bags, sealing the woven bags by needles and threads, and culturing at 25-30 ℃ for 2 days to be used for the inoculation of the fermented feed in the later period in mass production.

The effective storage period of the secondary strain is as follows: one month in summer, two months in spring and three months in winter.