阅读说明：本技术 一种含乳双歧杆菌bl-99的固体饮料及其应用 (Solid beverage containing bifidobacterium lactis BL-99 and application thereof ) 是由 刘伟贤 洪维鍊 赵雯 何剑 陈醇 于 2019-06-27 设计创作，主要内容包括：本发明公开一种含乳双歧杆菌BL-99的固体饮料及其应用。具体而言,本发明提供了一种固体饮料组合物,以固体饮料的总重量为100％计,其原料组成中包括：保藏编号CGMCC No.15650的乳双歧杆菌(Bifidobacterium lactis)或其活性物质0.1％～50％；麦芽糊精10％～20％。本发明的固体饮料具有促进肠道消化、通便的功效。(The invention discloses a solid beverage containing bifidobacterium lactis BL-99 and application thereof. Specifically, the invention provides a solid beverage composition, which comprises the following raw materials by weight percent based on 100 percent of the total weight of the solid beverage: bifidobacterium lactis (Bifidobacterium lactis) with the preservation number of CGMCC No.15650 or 0.1-50% of active substances thereof; 10 to 20 percent of maltodextrin. The solid beverage has the effects of promoting intestinal digestion and relaxing bowels.)

1. The solid beverage composition comprises the following raw materials by weight percent based on the total weight of the solid beverage as 100 percent:

bifidobacterium lactis (Bifidobacterium lactis) with the preservation number of CGMCC No.15650 or 0.1-50% of active substances thereof;

10 to 20 percent of maltodextrin.

2. The solid beverage composition according to claim 1, wherein the active substance is a fermentation culture product of Bifidobacterium lactis having a storage number of CGMCCNo.15650.

3. A solid beverage composition according to claim 1, wherein the Bifidobacterium lactis having a storage number of CGMCC No.15650 is contained in the beverage composition in an amount of 1.0X 10³CFU～1.0×10¹¹CFU/day, or 0.001 μ g-100 mg/day in terms of the weight of the cells.

4. The solid beverage composition according to claim 1, wherein the content of bifidobacterium lactis having a preservation number of CGMCC No.15650 in the solid beverage is 10⁸CFU～10¹⁰CFU/g, or 0.01 mu g-10 mg/day of thallus weight.

5. The solid beverage composition according to claim 1, wherein the solid beverage further comprises one or more of fructo-oligosaccharide, xylo-oligosaccharide, and erythritol.

6. A solid beverage composition according to claim 1 or 5, wherein the solid beverage further comprises fruit powder as the rest.

7. The solid beverage composition according to claim 1, which comprises the following raw materials by weight percentage based on the total weight of the solid beverage as 100 percent:

0.1-50% of bifidobacterium lactis or active substances thereof with the preservation number of CGMCC No. 15650;

10 to 20 percent of maltodextrin;

5 to 50 percent of fructo-oligosaccharide;

5 to 20 percent of xylo-oligosaccharide;

5% -20% of erythritol;

and (4) the balance of fruit powder.

8. A solid beverage composition according to claim 6 or 7, wherein the fruit powder is orange fruit powder, which is present in the solid beverage composition in an amount of 5% to 20%.

9. Use of the solid beverage composition according to any one of claims 1 to 8 for preparing a solid beverage having digestion promoting and/or laxative effects.

10. The use according to claim 9, wherein the bifidobacterium lactis having a accession number CGMCC No.15650 is present in the solid beverage in the form of a viable organism.

Technical Field

The invention relates to a solid beverage containing probiotics and application thereof, in particular to a solid beverage containing bifidobacterium lactis (BL-99) and application thereof in the aspects of promoting intestinal digestion, relaxing the bowels and the like.

Background

Today, with the pace of life increasing, irregular diets, frequent business trips, inadequate sleep, and inevitable alcohol consumption severely damage the digestive system of the body. While the imbalance of the digestive system is more likely to cause a series of intestinal problems: constipation, diarrhea, abdominal pain, borborborygmus, and … … air discharge, but if the intestinal peristalsis is slowed, the feces are retained in the intestinal tract and cannot be discharged in time, and harmful substances are generated in the feces in the intestinal tract, which seriously affects the human health. The results of the survey showed that 37.4% of 790 asian women suffered from constipation.

Researches show that the ingestion of probiotics can shorten the colon carrying time and simultaneously has certain help to regulate intestinal flora and improve gastrointestinal tract disorder of a subject. While colonic transit time may be defined as the time required for food to pass through swallowing, digestion, absorption and excretion. The health and morbidity of the gastrointestinal tract is sometimes associated with colonic transit time. Slow colonic transit represents symptoms such as constipation.

Disclosure of Invention

The invention discovers that Bifidobacterium lactis (Bifidobacterium lactis) BL-99 has the effect of promoting intestinal digestion, and accordingly provides a solid beverage containing the Bifidobacterium lactis BL-99.

According to the specific embodiment of the present invention, the bifidobacterium lactis BL-99 of the present invention has been deposited in 26.04.2018 in the china general microbiological culture collection center CGMCC (address: beijing, shangyang district, west way No.1 institute of microbiology, china academy of sciences), and is named by classification: bifidobacterium lactis (Bifidobacterium lactis); the preservation number is CGMCC No. 15650. Bifidobacterium lactis (BL-99) has gastric acid and intestinal juice resistance, and has viable bacteria survival rate of above 62% when treated in gastric acid solution with pH of 2.5 for 30min and viable bacteria survival rate of above 61% when treated for 2 hr; the survival rate of viable bacteria is more than 70 percent after the intestinal juice with pH6.8 is treated for 2 hours.

The study of the invention finds that a single strain of bifidobacterium lactis BL-99 (namely bifidobacterium lactis with the preservation number of CGMCC No. 15650) has the effect of promoting intestinal digestion, can promote intestinal peristalsis and increase the output of pepsin.

Further, the invention provides a solid beverage composition, which comprises the following raw materials by weight percentage of the total weight of the solid beverage as 100 percent:

bifidobacterium lactis (Bifidobacterium lactis) with the preservation number of CGMCC No.15650 or 0.1-50% of active substances thereof;

10 to 20 percent of maltodextrin.

According to a specific embodiment of the present invention, in the solid beverage composition of the present invention, the active substance is a fermentation culture product of bifidobacterium lactis with a preservation number of CGMCC No.15650, including but not limited to: a fermentation culture containing bacterial cells, a fermentation supernatant from which the bacterial cells have been removed, or an active substance extracted from the fermentation culture. Bifidobacterium lactis BL-99 of the present invention can be cultured by anaerobic fermentation in a culture medium for Bifidobacterium lactis (e.g., TPY medium, BBL medium, etc.) which is commonly used in the art. The optimal fermentation temperature is 35-38 ℃, and the optimal fermentation time is 7-24 h. The invention also provides a preparation method of the fermentation culture product of the bifidobacterium lactis BL-99, which comprises the step of carrying out anaerobic culture on the strain in a liquid fermentation culture medium to obtain a fermentation liquid containing the strain. The fermentation liquor can be directly used as a liquid bacterial preparation or further concentrated, and the fermentation liquor can be dried to prepare bacterial powder, or the bacterial powder is prepared by separating thalli from the fermentation liquor. The liquid bacterial preparation of the present invention may be a liquid bacterial preparation prepared by suspending the bacterial cells in a culture medium, a buffer solution, deionized water or other solvent. The BL-99 liquid bacterium preparation or the solid bacterium preparation (bacterium powder) can be stored in a viable bacterium form and has better stability in a storage period. The BL-99 liquid bacteria preparation or solid bacteria preparation (bacteria powder) of the present invention can also be preserved in the form of inactivated dead bacteria. The bacterial preparation can be used for preparing the solid beverage. According to a preferred embodiment of the invention, the bifidobacterium lactis having a accession number CGMCC No.15650 is present in the solid beverage in the form of a viable organism.

According to a specific embodiment of the invention, in the solid beverage composition of the invention, the content of the bifidobacterium lactis with the preservation number of CGMCC No.15650 in the beverage composition is 1.0 x 10³CFU～1.0×10¹¹CFU/day, or 0.001 μ g-100 mg/day in terms of the weight of the cells.

According to the inventionIn a preferred embodiment, in the solid beverage composition of the present invention, the content of bifidobacterium lactis having a preservation number of CGMCC No.15650 in the solid beverage is 10⁸CFU～10¹⁰CFU/g, or 0.01 mu g-10 mg/day of thallus weight.

According to an embodiment of the present invention, maltodextrin can be used to help maintain the viable count of the product during shelf life in the solid beverage composition of the present invention.

According to a specific embodiment of the present invention, some conventional components of the existing probiotic solid beverage can be further included in the solid beverage composition of the present invention. In some embodiments of the present invention, the raw material composition of the solid beverage of the present invention further comprises one or more of fructo-oligosaccharide, xylo-oligosaccharide, erythritol.

According to a particular embodiment of the invention, the solid beverage composition of the invention further comprises a balance of fruit powder.

In some embodiments of the present invention, the solid beverage composition of the present invention comprises the following raw materials, based on the total weight of the solid beverage as 100%:

0.1-50% of bifidobacterium lactis or active substances thereof with the preservation number of CGMCC No. 15650;

10 to 20 percent of maltodextrin;

5 to 50 percent of fructo-oligosaccharide;

5 to 20 percent of xylo-oligosaccharide;

5% -20% of erythritol;

and (4) the balance of fruit powder.

In some embodiments of the present invention, in the solid beverage composition of the present invention, the fruit powder is orange fruit powder, and the content of the fruit powder in the solid beverage composition is 5% to 20%.

The preparation method of the solid beverage can be carried out by referring to the existing preparation technology of the probiotic solid beverage. In general, the solid beverage of the present invention can be obtained by uniformly mixing the components of the composition.

In another aspect, the invention also provides the application of the solid beverage composition in preparing a solid beverage with digestion promoting and/or constipation relieving effects.

The solid beverage of the invention has the corresponding effect of promoting intestinal digestion due to the inclusion of the bifidobacterium lactis BL-99, and can promote intestinal peristalsis and increase the output of pepsin. The solid beverage has better defecation effect.

Drawings

FIG. 1 shows the effect of Bifidobacterium lactis BL-99 on the ink propulsion rate (%).

FIG. 2 shows the effect of Bifidobacterium lactis BL-99 on the gastric fluid volume in rats.

FIG. 3 shows the effect of Bifidobacterium lactis BL-99 on rat pepsin activity.

FIG. 4 shows the effect of Bifidobacterium lactis BL-99 on pepsin output in rats.

FIG. 5 is a schematic view of a fermentation process according to an embodiment of the present invention.

FIG. 6 shows the effect of BL-99 solid drink of the present invention on defecation and digestion (results of human test questionnaire).

Microbial preservation of the patent procedure:

bifidobacterium lactis BL-99 of the present invention:

the preservation date is as follows: 26/04/2018;

the preservation unit: china general microbiological culture Collection center (CGMCC);

the address of the depository: xilu No.1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang

The preservation number is: CGMCC No. 15650;

and (3) classification and naming: bifidobacterium lactis (Bifidobacterium lactis).

Detailed Description

For a more clear understanding of the technical features, objects and advantages of the present invention, reference is now made to the following detailed description taken in conjunction with the accompanying specific embodiments, and the technical solutions of the present invention are described, it being understood that these examples are intended to illustrate the present invention and are not intended to limit the scope of the present invention. In the examples, each raw reagent material is commercially available, and the experimental method without specifying the specific conditions is a conventional method and a conventional condition well known in the art, or a condition recommended by an instrument manufacturer.

Unless specifically defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the relevant art. Unless otherwise indicated, all numbers expressing quantities of ingredients, cell culture, processing conditions, and so forth used in the present disclosure are to be understood as being modified in all instances by the term "about". Accordingly, unless indicated to the contrary, the numerical parameters are approximations and may vary depending upon the desired properties sought to be obtained by the present invention. The term "at least" preceding a series of elements is to be understood as referring to each element in the series, unless otherwise indicated.

In various examples of the invention, experimental data are expressed as Mean ± s.e.m. data were counted using PRISM version5.0(GraphPad, San Diego, CA, USA). Differences between groups were counted using one-way ANOVA followed by Tukery's smart composition test. There were significant statistical differences at P < 0.05.