阅读说明：本技术 一种基于pl级压电喷头技术活性干细胞微3d打印装置 (Active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology ) 是由 周夫之 李跃斌 朱纬 邓肖 于 2019-10-18 设计创作，主要内容包括：本发明涉及生物细胞3D打印技术领域,具体来说是一种基于PL级压电喷头技术活性干细胞微3D打印装置,其特征在于包括若干压电喷头,若干细胞储液瓶,所述压电喷头的流体通道采用毛细玻璃管与细胞储液瓶连通,高粘度材料挤出装置,采用螺杆式挤出阀,视觉观测装置,由水平观测装置和竖直观测装置组成,水平观测装置用于水平方向压电喷头的细胞打印检测,竖直观测装置用于打印材料的定位打印校准及打印效果检测,其优点在于：使用喷墨打印技术替代传统的挤出式方法进行细胞打印,能够实现挤出式方法无法做到的单细胞打印,同时细胞的精度高,能够做到微米级。(The invention relates to the technical field of biological cell 3D printing, in particular to an active stem cell micro 3D printing device based on a PL-level piezoelectric nozzle technology, which is characterized by comprising a plurality of piezoelectric nozzles and a plurality of cell liquid storage bottles, wherein fluid channels of the piezoelectric nozzles are communicated with the cell liquid storage bottles by capillary glass tubes, a high-viscosity material extrusion device is composed of a horizontal observation device and a vertical observation device by a screw extrusion valve, the horizontal observation device is used for cell printing detection of the piezoelectric nozzles in the horizontal direction, and the vertical observation device is used for positioning printing calibration and printing effect detection of printing materials, and has the advantages that: the ink-jet printing technology is used for replacing the traditional extrusion method to print the cells, single-cell printing which cannot be realized by the extrusion method can be realized, meanwhile, the precision of the cells is high, and micron-scale printing can be realized.)

1. Active stem cell micro 3D printing device based on PL-level piezoelectric nozzle technology, and is characterized in that

Comprises a plurality of piezoelectric spray heads,

a plurality of cell liquid storage bottles are arranged,

the fluid channel of the piezoelectric nozzle is communicated with a cell liquid storage bottle by adopting a capillary glass tube,

a high-viscosity material extrusion device adopts a screw type extrusion valve, an extrusion valve control motor is connected with a controller positioned in an electric cabinet, the controller controls the rotation speed and the direction of the motor, the extrusion valve control motor is connected with a transmission screw to synchronously rotate so as to promote an extrusion valve slide block to reciprocate along the direction of the transmission screw, a storage barrel is used for storing liquid materials to be printed, an injection plug is connected with the extrusion valve slide block to synchronously move so as to extrude the liquid materials to be printed from an injection nozzle,

the visual observation device consists of a horizontal observation device and a vertical observation device, wherein the horizontal observation device is used for cell printing detection of the piezoelectric nozzle in the horizontal direction, and the vertical observation device is used for positioning, printing, calibrating and printing effect detection of a printing material.

2. The active stem cell micro 3D printing device based on PL-level piezojet technology as claimed in claim 1, wherein: the portal frame is installed on mounting plate, vertical motion module mounting panel is installed on the portal frame, vertical motion module is installed on vertical motion module mounting panel, left side vertical motion module slider can reciprocate, be used for reciprocating single cell inkjet printing system, right side vertical motion module slider can reciprocate, be used for reciprocating high viscosity material extrusion system, the longitudinal motion module is installed on mounting plate, the transverse motion module is installed on longitudinal motion module slider, it installs on transverse motion module slider to print the bottom plate, transverse motion module slider can control about can, longitudinal motion module slider can the back-and-forth movement, transverse motion module and longitudinal motion module are used for printing bottom plate all around the plane removal.

3. The active stem cell micro 3D printing device based on PL-level piezojet technology as claimed in claim 1, wherein: the screw type extrusion valve is installed on the right side vertical motion module slider, the slider can reciprocate, the curing lamp installation block is installed on the right side vertical motion module slider, the ultraviolet curing lamp is installed on the curing lamp installation block, the strobe lamp is installed on the curing lamp installation block, and the curing lamp installation block reciprocates along with the right side vertical motion module slider.

4. The active stem cell micro 3D printing device based on PL-level piezojet technology as claimed in claim 2, wherein: be equipped with vertical mounting panel on the vertical motion module slider in left side, the slider can reciprocate, vertical camera installation piece is installed on vertical mounting panel, vertical zoom lens is installed on vertical camera installation piece, vertical camera is installed on vertical zoom lens, vertical mounting panel still is equipped with liquid reserve bottle mounting bracket, shower nozzle mounting bracket and air cleaner, be equipped with four shower nozzle installation stations on the shower nozzle mounting bracket, be used for installing piezoelectric nozzle, be equipped with four installation stations on the liquid reserve bottle mounting bracket, be used for installing the liquid reserve bottle, air cleaner and cell liquid reserve bottle are connected to the trachea, piezoelectric nozzle and cell liquid reserve bottle are connected to the liquid way pipe, shower nozzle signal interface installs on vertical mounting panel, piezoelectric nozzle signal line connection shower nozzle signal interface.

5. The active stem cell micro 3D printing device based on PL-level piezojet technology as claimed in claim 2, wherein: the vertical motion module mounting panel on be equipped with horizontal camera mounting bracket, horizontal camera motion module is installed on horizontal camera mounting bracket, horizontal camera is installed on horizontal camera motion module, horizontal camera lens is installed on horizontal camera.

Technical Field

The invention relates to the technical field of 3D printing of biological cells, in particular to an active stem cell micro 3D printing device based on a PL-level piezoelectric nozzle technology.

Background

The 3D printing technology is a rapid prototyping technology which is popularized and developed in recent years, and is widely applied to industrial production, and the 3D printing technology can be used as research and development equipment for sample printing and active organ printing of biological organs in the biomedical industry because the 3D printing technology can be used for carrying out complex graphic printing based on a computer three-dimensional model, and is suitable for assembling biological materials or living cells to construct living tissues in vitro. The traditional biological 3D printing device mainly adopts an extrusion method for cell printing, and although the inkjet printing technology is also used, the inkjet printing technology is only used as a selective configuration due to the technical limitations of manufacturers, and does not achieve effective popularization. The extrusion method is widely used at present, the molding precision is in the order of hundreds of microns or millimeter, the volume of a single cell is in the order of microns, the extrusion method cannot perform accurate printing of single cells, the printing precision of the ink-jet printing technology is dozens of microns to hundreds of microns, and effective printing of single cells can be obtained.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides an active stem cell micro 3D printing device capable of realizing accurate single cell printing.

In order to achieve the purpose, the active stem cell micro 3D printing device based on the PL-level piezoelectric nozzle technology is designed, and is characterized by comprising a plurality of piezoelectric nozzles and a plurality of cell liquid storage bottles, wherein fluid channels of the piezoelectric nozzles are communicated with the cell liquid storage bottles through capillary glass tubes, a high-viscosity material extrusion device adopts a screw type extrusion valve, an extrusion valve control motor 29 is connected with a controller in an electric cabinet, the controller controls the rotation speed and the direction of the motor, the extrusion valve control motor 29 is connected with a transmission screw 31 to synchronously rotate, so that an extrusion valve slide block 30 is driven to reciprocate along the direction of the transmission screw 31, a storage barrel 32 is used for storing liquid materials to be printed, an injection plug 33 is connected with the extrusion valve slide block 30 to synchronously move, the liquid materials to be printed are extruded from an injection nozzle 34, and a visual observation device consists of a horizontal observation device and a vertical observation device, the horizontal observation device is used for cell printing detection of the piezoelectric nozzle in the horizontal direction, and the vertical observation device is used for positioning, printing calibration and printing effect detection of a printing material.

The portal frame is installed on mounting plate, vertical motion module mounting panel is installed on the portal frame, vertical motion module is installed on vertical motion module mounting panel, left side vertical motion module slider can reciprocate, be used for reciprocating single cell inkjet printing system, right side vertical motion module slider can reciprocate, be used for reciprocating high viscosity material extrusion system, the longitudinal motion module is installed on mounting plate, the transverse motion module is installed on longitudinal motion module slider, it installs on transverse motion module slider to print the bottom plate, transverse motion module slider can control about can, longitudinal motion module slider can the back-and-forth movement, transverse motion module and longitudinal motion module are used for printing bottom plate all around the plane removal.

The screw type extrusion valve is installed on the right side vertical motion module slider, the slider can reciprocate, the curing lamp installation block is installed on the right side vertical motion module slider, the ultraviolet curing lamp is installed on the curing lamp installation block, the strobe lamp is installed on the curing lamp installation block, and the curing lamp installation block reciprocates along with the right side vertical motion module slider.

Be equipped with vertical mounting panel on the vertical motion module slider in left side, the slider can reciprocate, vertical camera installation piece is installed on vertical mounting panel, vertical zoom lens is installed on vertical camera installation piece, vertical camera is installed on vertical zoom lens, vertical mounting panel still is equipped with liquid reserve bottle mounting bracket, shower nozzle mounting bracket and air cleaner, be equipped with four shower nozzle installation stations on the shower nozzle mounting bracket, be used for installing piezoelectric nozzle, be equipped with four installation stations on the liquid reserve bottle mounting bracket, be used for installing the liquid reserve bottle, air cleaner and cell liquid reserve bottle are connected to the trachea, piezoelectric nozzle and cell liquid reserve bottle are connected to the liquid way pipe, shower nozzle signal interface installs on vertical mounting panel, piezoelectric nozzle signal line connection shower nozzle signal interface.

The vertical motion module mounting panel on be equipped with horizontal camera mounting bracket, horizontal camera motion module is installed on horizontal camera mounting bracket, horizontal camera is installed on horizontal camera motion module, horizontal camera lens is installed on horizontal camera.

Compared with the prior art, the invention has the advantages that: the ink-jet printing technology is used for replacing the traditional extrusion method to print the cells, single-cell printing which cannot be realized by the extrusion method can be realized, meanwhile, the precision of the cells is high, and micron-scale printing can be realized.

Drawings

FIG. 1 is a schematic view of the structure of the present invention 1;

FIG. 2 is a schematic structural diagram of the present invention 2;

in the figure: 1. the left Z motion module 2, the right Z motion module 3, the screw type extrusion valve 4, the Z motion module mounting plate 5, the portal frame 6, the mounting base plate 7, the vertical camera 8, the vertical zoom lens 9, the horizontal camera mounting frame 10, the horizontal camera motion module 11, the horizontal camera 12, the horizontal lens 13, the piezoelectric nozzle 14, the nozzle mounting frame 15, the printing base plate 16, the X motion module 17, the Y motion module 18, the cell liquid storage bottle 19, the ultraviolet curing lamp 20, the curing lamp mounting block 21, the strobe lamp 22, the air filter 23, the air pipe 24, the liquid storage bottle mounting frame 25, the nozzle signal interface 26, the liquid pipe 27, the vertical camera mounting block 28 and the Z mounting plate.

Detailed Description

The construction and principles of such a device will be apparent to those skilled in the art from the following further description of the invention taken in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

As shown in fig. 1 and 2, the active stem cell micro 3D printing system based on the PL-level piezojet technology comprises a motion control system consisting of a left Z motion module 1, a right Z motion module 2, a Z motion module mounting plate 4, a gantry 5, a mounting base plate 6, a printing base plate 15, an X motion module 16 and a Y motion module 17; the visual observation system consists of a vertical camera 7, a vertical zoom lens 8, a horizontal camera mounting frame 9, a horizontal camera motion module 10, a horizontal camera 11 and a horizontal lens 12; the single-cell ink-jet printing system comprises an ink-jet nozzle 13, a nozzle mounting frame 14, a cell liquid storage bottle 18, a strobe light 21, an air filter 22, an air pipe 23, a liquid storage bottle mounting frame 24, a nozzle signal interface 25 and a liquid path pipe 26; and a screw type extrusion valve 3, an ultraviolet curing lamp 19 and a curing lamp mounting block 20.

As shown in the figure I, a portal frame 5 is arranged on an installation bottom plate, a Z motion module installation plate 4 is arranged on the portal frame 5, a left Z motion module 1 and a right Z motion module 2 are arranged on the Z motion module installation plate 4, and a slide block of the left Z motion module 1 can move up and down and is used for moving up and down a single-cell ink-jet printing system; the right Z-shaped moving module 2 is provided with a slide block which can move up and down and is used for moving the high-viscosity material extrusion system up and down; the Y motion module 17 is installed on the installation bottom plate 6, the X motion module 16 is installed on a Y motion module 17 slide block, the printing bottom plate 15 is installed on an X motion module 16 slide block, the X motion module 16 slide block can move left and right, the Y motion module 17 slide block can move front and back, and the X motion module 16 and the Y motion module 17 are used for moving the printing bottom plate 15 in front, back, left and right planes.

As shown in fig. 1, the screw type extrusion valve 3 is arranged on a slide block of the right Z motion module 2 and can move up and down; curing lamp installation piece 20 is installed on 2 sliders of right side Z motion module, and ultraviolet curing lamp 19 is installed on curing lamp installation piece 20, and strobe light 21 is installed on curing lamp installation piece 20, and curing lamp installation piece 20 can follow 2 sliders of right side Z motion module and reciprocate.

As shown in fig. 1 and 2, the Z mounting plate 28 is mounted on the left Z motion module 1 slide block and can move up and down; the vertical camera mounting block 27 is mounted on the Z mounting plate 28, the vertical zoom lens 8 is mounted on the vertical camera mounting block 27, the vertical camera 7 is mounted on the vertical zoom lens 8, and the vertical zoom lens 8 includes a coaxial light source; the liquid storage bottle mounting rack 24 is mounted on the Z mounting plate 28, the spray head mounting rack 14 is mounted on the Z mounting plate 28, the ink jet spray head 13 is mounted on the spray head mounting rack 14, and the spray head mounting rack 14 is provided with four spray head mounting stations for mounting the spray head; cell stock solution bottle 18 is installed on stock solution bottle mounting bracket 24, and stock solution bottle mounting bracket 24 has 4 installation work stations to be used for installing stock solution bottle 18, and air cleaner 22 installs on Z mounting panel 28, and air cleaner 22 and cell stock solution bottle 18 are connected to trachea 23, and liquid way pipe 26 connects inkjet nozzle 13 and cell stock solution bottle 18, and shower nozzle signal interface 25 installs on Z mounting panel 28, and shower nozzle signal interface 25 is connected to inkjet nozzle 13 signal line.

As shown in fig. 1 and 2, the horizontal camera mounting bracket 9 is mounted on the Z motion module mounting plate 4, the horizontal camera motion module 10 is mounted on the horizontal camera mounting bracket 9, the horizontal camera 11 is mounted on the horizontal camera motion module 10, and the horizontal lens 12 is mounted on the horizontal camera 11.

The operation method comprises the following specific steps:

adding cell solution into 18 cell liquid storage bottles, adjusting parameters of an ink jet head 13 through a computer, wherein the ink jet head 13 can form stable liquid drops, and observing the state of the liquid drops through a horizontal camera 11 and a horizontal lens 12. the invention has the advantages that the ink jet head 11 adopts a single-channel head, the related pipelines are Teflon and capillary glass tubes, 4 cells can be simultaneously printed in a single-process manner, the horizontal camera 11 and the horizontal lens 12 can move back and forth under the action of a horizontal camera mounting rack, and the 4 cell printing states can be observed; materials are added into the screw type extrusion valve 3, parameters of the materials are adjusted through a computer, and a motion program is edited through upper computer software.

The extrusion valve control motor is connected with a controller located in the electric cabinet, the controller can accurately control the rotating speed and the rotating direction of the motor, the extrusion valve control motor is connected with the transmission screw to rotate synchronously, the extrusion valve sliding block is made to reciprocate along the direction of the transmission screw, the material storage barrel is used for storing liquid materials to be printed, the injection plug is connected with the extrusion valve sliding block to move synchronously, and the liquid materials to be printed are extruded from the injection nozzle.

The screw type extrusion valve is used for printing high-viscosity liquid materials, 3D graph printing can be achieved through accurate control of a computer in cooperation with a motion control system, and the printed 3D model serves as a cell survival carrier so that subsequent ink jet cells can be printed accurately.