阅读说明：本技术 一种低含量甘草霜负载凹凸棒石抗菌饲料的制备方法 (Preparation method of low-content liquorice cream loaded attapulgite antibacterial feed ) 是由 董树清 王爱勤 康玉茹 刘红 邵士俊 于 2019-12-12 设计创作，主要内容包括：本发明提供了一种低含量甘草霜负载凹凸棒石抗菌饲料的制备方法,是将甘草先采用常规的水提醇沉或醇提水沉的方式进行粗提,再通过大孔树脂纯化,即得低含量甘草霜；再利用凹凸棒石与低含量甘草霜负载技术制备了低含量甘草霜负载凹凸棒石抗菌饲料完成负载型抗菌材料。本发明不仅充分应用天然产物甘草霜易于降解、绿色环保的优势,而且充分利用凹凸棒石的吸附性和生物兼容性,使得甘草霜与凹凸棒石的优势互补,为天然抗菌饲料的开发提供辅料。实验表明,本发明制备的低含量甘草霜负载凹凸棒石抗菌饲料对大肠杆菌或金黄色葡萄球菌具有较强的抑菌效果,作为添加辅料添加到饲料基质中,是一种非常有前景的抗菌饲料。(The invention provides a preparation method of a low-content licorice cream loaded attapulgite antibacterial feed, which comprises the steps of firstly, carrying out crude extraction on licorice by adopting a conventional water extraction and alcohol precipitation or alcohol extraction and water precipitation mode, and then purifying by macroporous resin to obtain the low-content licorice cream; and then the attapulgite and low-content licorice cream loading technology is utilized to prepare the low-content licorice cream loaded attapulgite antibacterial feed to finish the loading type antibacterial material. The invention not only fully applies the advantages of easy degradation and environmental protection of the natural product liquorice frost, but also fully utilizes the adsorbability and the biocompatibility of the attapulgite, so that the advantages of the liquorice frost and the attapulgite are complementary, and auxiliary materials are provided for the development of natural antibacterial feed. Experiments show that the low-content liquorice frost-loaded attapulgite antibacterial feed has a strong antibacterial effect on escherichia coli or staphylococcus aureus, is added into a feed matrix as an additive auxiliary material, and is a very promising antibacterial feed.)

1. A preparation method of a low-content liquorice frost-loaded attapulgite antibacterial feed comprises the following process steps:

(1) extracting and purifying the low-content liquorice cream: extracting Glycyrrhrizae radix with water, precipitating with ethanol or extracting with ethanol and precipitating with water, and purifying with macroporous resin to obtain low-content Glycyrrhrizae radix cream;

(2) preparation of the load type attapulgite antibacterial material: adding the purified attapulgite and the dispersing agent into distilled water, and performing ultrasonic oscillation to form attapulgite dispersion liquid; dissolving the extracted low-content licorice cream in distilled water, adding the solution into the attapulgite dispersion, stirring at normal temperature for 0.5-2 h, adjusting the pH value of the system to 8-12, and filtering, washing and drying the product to obtain the attapulgite-low-content licorice cream antibacterial material.

2. The preparation method of the low-content licorice cream loaded attapulgite antibacterial feed as claimed in claim 1, wherein the preparation method comprises the following steps: the macroporous resin adopts D-101, AB-8 and LSD-001.

3. The preparation method of the low-content licorice cream loaded attapulgite antibacterial feed as claimed in claim 1, wherein the preparation method comprises the following steps: the mass ratio of the attapulgite to the low-content licorice cream is 1: 1-8: 1.

4. The preparation method of the low-content licorice cream loaded attapulgite antibacterial feed as claimed in claim 1, wherein the preparation method comprises the following steps: the dispersant is SiO₂Calcium formate, calcium stearate.

5. The preparation method of the low-content licorice cream loaded attapulgite antibacterial feed as claimed in claim 4, wherein the addition amount of the dispersing agent is 0.5-5% of the mass of the attapulgite.

Technical Field

The invention relates to a natural antibacterial feed, in particular to a preparation method of a low-content licorice cream loaded attapulgite antibacterial feed, belonging to the technical field of composite materials, nano antibacterial materials and plant-source antibacterial agents.

Background

In recent years, research on plant-derived antibacterial agents has been greatly advanced at home and abroad. At present, about 2000 plants can be used for extracting and preparing the antibacterial agent. The substances with plant-derived antibacterial activity mainly comprise terpenes, alkaloids, flavonoids, phenols, quinones, aldehydes, alcohols, phenylpropanoids, spices, lignans, steroids, organic acids, essential oils and the like. The liquorice is a plant with homology of medicine and food in liquorice of leguminosae, is rich in resources in China, and has the effects of protecting the liver, resisting the ulcer, resisting the tumor, protecting the nerve and the like. Because active source substances in the liquorice are mainly triterpenoid saponin and flavonoid compounds, the research on the anti-tumor and anti-oxidation activity of the liquorice is wide. With the rapid development of the natural product chemistry, the chemical components of the natural product are continuously and deeply studied, and the liquorice also has the activities of resisting the ulcer, inflammation, bacteria and the like. The literature reports that liquorice has better antibacterial effect on bacteria such as escherichia coli, staphylococcus aureus, beta hemolytic streptococcus and the like. Therefore, the extraction and preparation of the natural antibacterial plant source substances in the liquorice are developed, and the research of the liquorice extract and related products is also a hotspot.

The attapulgite is a natural green nano antibacterial material, and has a special pore structure, stability and biocompatibility. The liquorice extract is loaded on the attapulgite by adopting a loading adsorption mode, and the adsorption property and the biocompatibility of the attapulgite can be fully utilized, so that auxiliary materials are provided for the development of natural antibacterial feed.

Disclosure of Invention

The invention aims to provide a preparation method of a low-content liquorice cream loaded attapulgite antibacterial feed;

the invention adopts a biosynthesis technology to complete the extraction of the licorice extract-low-content licorice cream, and utilizes an attapulgite and low-content licorice cream loading technology to complete the green synthesis of the loaded antibacterial material and improve the antibacterial adsorption performance of the attapulgite. The method specifically comprises the following process steps:

(1) extracting and purifying the low-content liquorice cream: the liquorice is firstly subjected to crude extraction by adopting a conventional water extraction and alcohol precipitation or alcohol extraction and water precipitation method, and then is purified by macroporous resin, so that the low-content liquorice cream is obtained. Wherein the macroporous resin adopted for extraction is D-101, AB-8 and LSD-001. The extract is yellow fine powder, odorless, and has special sweet taste;

(2) preparation of the load type attapulgite antibacterial material: adding the purified attapulgite and the dispersing agent into distilled water, and performing ultrasonic oscillation to form attapulgite dispersion liquid; dissolving the extracted low-content licorice cream in distilled water, adding the solution into the attapulgite dispersion, stirring at normal temperature for 0.5-2 h, adjusting the pH value of the system to 8-12, and filtering, washing and drying the product to obtain the attapulgite-low-content licorice cream antibacterial material.

The dispersing agent adopts SiO₂Calcium formate, calcium stearate; the addition amount of the dispersing agent is 0.5-5% of the mass of the attapulgite. The mass ratio of the attapulgite to the low-content licorice cream is 1: 1-8: 1.

The prepared low-content liquorice frost-loaded attapulgite antibacterial feed not only fully applies the advantages of easy degradation and environmental protection of natural product liquorice frost, but also fully utilizes the adsorbability and biocompatibility of attapulgite to complement the advantages of the liquorice frost and the attapulgite, thereby providing auxiliary materials for the development of natural antibacterial feed. Experiments show that the low-content liquorice frost-loaded attapulgite antibacterial feed has a strong antibacterial effect on escherichia coli or staphylococcus aureus, is added into a feed matrix as an additive auxiliary material, and is a very promising antibacterial feed.

Detailed Description

The preparation and antibacterial performance of the low-content licorice cream loaded attapulgite antibacterial material are further explained by the specific examples.