Plant source harmonia axyridis repellent
阅读说明：本技术 植物源异色瓢虫趋避剂 (Plant source harmonia axyridis repellent ) 是由 石旺鹏 秦紫芳 赵欣欣 徐毓笛 汪洋 于 2019-12-04 设计创作，主要内容包括：本发明提供一种植物源异色瓢虫趋避剂。本发明通过实验表明：乙酰基吡嗪在10<Sup>-4</Sup>g/mL、10<Sup>-</Sup><Sup>5</Sup>g/mL浓度下对异色瓢虫雌虫具有显著的驱避作用；薄荷酮在10<Sup>-6</Sup>g/mL浓度下对异色瓢虫雌虫具有显著的驱避作用；庚烷-2-酮在10<Sup>-2</Sup>g/mL、10<Sup>-</Sup><Sup>3</Sup>g/mL、10<Sup>-4</Sup>g/mL浓度下均对异色瓢虫有显著的驱避作用,并且驱避率在庚烷-2-酮浓度为10<Sup>-3</Sup>g/mL时达到最高78.82％。因此,特定浓度的乙酰基吡嗪、薄荷酮或庚烷-2-酮可作为异色瓢虫的趋避剂。本发明通过室内行为学技术研究了异色瓢虫对多种植物源物质的不同浓度的趋性,筛选出异色瓢虫的驱避剂,为发展异色瓢虫的保护利用与行为调控技术对策提供了科学依据。(The invention provides a botanical harmonia axyridis repellent. Experiments show that: acetylpyrazines are present in 10 ‑4 g/mL、10 ‑ 5 The insecticidal composition has obvious repellent effect on female harmonia axyridis at g/mL concentration; menthone is in 10 ‑6 The insecticidal composition has obvious repellent effect on female harmonia axyridis at g/mL concentration; heptane-2-one in 10 ‑2 g/mL、10 ‑ 3 g/mL、10 ‑4 Has remarkable repelling effect on harmonia axyridis under the concentration of g/mL, and the repelling rate is that the concentration of heptane-2-ketone is 10 ‑3 A maximum of 78.82% was reached at g/mL. Therefore, acetylpyrazine, menthone or heptane-2-ketone at specific concentrations can be used as repellent for harmonia axyridis. The invention is provided withThe indoor ethology technology researches the tropism of the harmonia axyridis to different concentrations of various plant-derived substances, screens out the repellent of the harmonia axyridis, and provides scientific basis for developing protective utilization and behavior regulation and control technical measures of the harmonia axyridis.)
1. A botanical ladybug repellent agent comprises: a solution of a mixture of one or more of acetylpyrazine, menthone and heptan-2-one;
the solution adopts a cosolvent of a plant source pesticide as a solvent; specifically, liquid paraffin, n-hexane, and dichloromethane can be used.
2. The plant-derived harmonia axyridis repellent according to claim 1, wherein: the harmonia axyridis repellent is acetyl pyrazine solution,
in the acetylpyrazine solution, the concentration of the acetylpyrazine is 10-4g/mL-10-5g/mL。
3. The plant-derived harmonia axyridis repellent according to claim 1, wherein: the harmonia axyridis repellent is menthone solution,
the concentration of menthone in the menthone solution is equal to or lower than 10-6g/mL。
4. The plant-derived harmonia axyridis repellent according to claim 1, wherein: the harmonia axyridis repellent is heptane-2-ketone solution,
the heptane-2-ketone solution has a heptane-2-ketone concentration of 10-2g/mL-10-4g/mL, specifically 10-3g/mL。
5. Application of acetylpyrazine as diaphoresis repellent or in preparation of diaphoresis repellent.
6. Use according to claim 5, characterized in that: acetylpyrazine concentration is 10-4g/mL-10-5g/mL；
The harmonia axyridis repellent agent is a harmonia axyridis female repellent agent.
7. Application of menthone as diaphoresis repellent agent or in preparation of diaphoresis repellent agent.
8. Use according to claim 7, characterized in that: the concentration of menthone is 10 or less-6g/mL; the harmonia axyridis repellent agent is a harmonia axyridis female repellent agent.
9. The heptane-2-ketone is used as the repellent agent of the harmonia axyridis or is applied to the preparation of the repellent agent of the harmonia axyridis.
10. Use according to claim 9, characterized in that: the concentration of heptane-2-one is 10-2g/mL-10-4g/mL, specifically 10-3g/mL。
Technical Field
The invention belongs to the field of plant protection, and particularly relates to an information substance screening method capable of avoiding natural enemy insect harmonia axyridis and an application method thereof.
Background
Harmonia axyridis (Pallas) belongs to the family of Coleoptera ladybridae, is a dominant predatory natural enemy in the agricultural ecosystem of China, can predate various pests such as aphids, mites and scale insects on crops such as wheat, corn, cotton, vegetables and fruit trees, plays an important role in regulating pest populations, and plays an important role in maintaining ecological balance. The ladybug has phototaxis, so a large amount of ladybugs are attracted in the insect light collection and pest trapping and killing processes, the ladybugs are greatly killed, the healthy development of an ecological system and the sustainable control of crop pests are not facilitated, the research on ladybug avoidance information substances is very necessary for protecting the ladybug and avoiding the light trapping and killing, and the ladybug is of great significance to beneficial insect protection and pest control.
Acetylpyrazine substances are important intermediates for synthesizing medicaments and pesticides, and are also flavoring agents (von aceseries. brief introduction of research and development of acetylpyrazine substances [ J ]. Shanxi chemical industry, 1984(03): 52-53);
the chiral isomers of menthone have certain repellent and lethal activities on culex pipiens pallens (para-epilia, plum-shin-shou, hengqin, zhuxing. carvone and the chiral isomers of menthone have fumigating and repellent activities on culex pipiens palliens [ J ]. Anxus, 2016,59(07): 732-738.);
the camphor ball can be used for repelling mosquitoes (Linlihui, Fangmeiyu, Zhouyilong, Li Xin, Wei Jia, Wei Hai. Compound camphor gel repellent evaluation of efficacy and safety [ J ]. China media biology and control journal, 2002(06):413 + 415), codling moth (Li Jianglin. hanging camphor tablet to repel codling moth [ J ]. Xinjiang agricultural science, 1987(04):26.), larch cone fly (strictly good spring, Sun Jianghua, late Rich, Zhang Dandan, Zhao Qikai.) and the repellent effect of plant volatile substances on larch cone fly [ J ]. ecology news, 2003(02):314 + 319);
carboxamides are broad-spectrum repellents and can repel mosquitoes (a specific effect), cockroaches, fleas, midges, gadflies, sandfleas, sandmites, deer flies, sand flies (Wang, B js. an intervening and Successful Organic Experiment [ J ]. Journal of Chemical eduction, 1974,51), and the like.
In order to develop the protection utilization and behavior regulation technical strategy of the harmonia axyridis, substances with the evasion function to the harmonia axyridis need to be researched and screened.
Disclosure of Invention
The invention provides a botanical harmonia axyridis repellent.
The botanical harmonia axyridis repellent provided by the invention can be: a solution of a mixture of one or more of acetylpyrazine, menthone and heptan-2-one;
the solution can adopt cosolvent of plant pesticide as solvent, specifically liquid paraffin, n-hexane, dichloromethane and the like, and more specifically liquid paraffin;
the harmonia axyridis repellent is acetylpyrazine solution, wherein the concentration of acetylpyrazine in the acetylpyrazine solution can be 10-4g/mL-10-5g/mL；
The harmonia axyridis repellent is a menthone solution, and the concentration of the menthone in the menthone solution is equal to or lower than 10-6g/mL; may be specifically 10-6g/mL；
The harmonia axyridis repellent is heptane-2-ketone solution, and the concentration of heptane-2-ketone in the heptane-2-ketone solution can be 10-2g/mL-10-4g/mL, specifically 10-2g/mL-10-3g/mL、10-3g/mL-10-4g/mL、10-2g/mL、10-3g/mL or 10-4g/mL, more specifically can be 10-3g/mL。
The invention also aims to provide the application of the plant-derived substance as the harmonia axyridis repellent or in the preparation of the harmonia axyridis repellent.
In the application, the plant source substance is acetylpyrazine, and the concentration of the acetylpyrazine can be 10-4g/mL-10-5g/mL；
The plant source substance is menthone, and the concentration of the menthone is equal to or lower than 10-6g/mL；
The plant source substance is heptane-2-ketone, and the plant source substance isThe concentration of heptane-2-one may be 10-2g/mL-10-4g/mL, specifically 10-2g/mL-10-3g/mL、10-3g/mL-10-4g/mL、10-2g/mL、10-3g/mL or 10-4g/mL, more specifically can be 10-3g/mL；
The coccinella septempunctata repellent can be specifically coccinella septempunctata female repellent.
Experiments show that: acetylpyrazines are present in 10-4g/mL，10-5The insecticidal composition has obvious repellent effect on female harmonia axyridis at g/mL concentration; menthone is in 10-6The insecticidal composition has obvious repellent effect on female harmonia axyridis under the concentration of g/mL; heptane-2-one in 10-2Has remarkable repelling effect on female harmonia axyridis at the concentration of 10 g/mL-3g/mL、10-4Has extremely obvious repellent effect on female harmonia axyridis under the concentration of g/mL, and particularly, the concentration of heptane-2-ketone is 10-3The maximum repellent rate reaches 78.82% when the concentration is g/mL.
The method researches the tropism of the harmonia axyridis to different concentrations of various plant-derived substances through an indoor behavioristics technology, screens out the repellent of the harmonia axyridis, and provides scientific basis for developing protective utilization and behavior regulation and control technical countermeasures of the harmonia axyridis.
Drawings
FIG. 1 reactivity of harmonia axyridis (female) with different concentrations of acetylpyrazine.
FIG. 2 the response rate of harmonia axyridis (female) to different concentrations of menthone.
FIG. 3 reactivity of harmonia axyridis (female) with different concentrations of heptan-2-one.
FIG. 4 reactivity of harmonia axyridis (female) with formamide at different concentrations.
Detailed Description
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified.
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