阅读说明：本技术 季铵盐臭氧油及其制备方法 (Quaternary ammonium salt ozone oil and preparation method thereof ) 是由 尹芬芳 于 2019-11-25 设计创作，主要内容包括：本发明涉及一种季铵盐臭氧油及其制备方法。所述季铵盐臭氧油的制备原料包括壳聚糖季铵盐、橄榄油和臭氧；每500mL所述橄榄油对应的所述壳聚糖季铵盐的用量为2g-5g,以及对应的所述臭氧的用量为12g-25g。本发明将适量的橄榄油、臭氧和壳聚糖季铵盐搭配使用,形成特定的杀菌消毒配方,用该配方对创伤组织进行杀菌消毒时,三种成分协同增效,具有很强的杀菌消毒效果和强氧化能力。并且,发明人发现,该配方在口服过程中,不会对创伤组织周围的健康组织带来伤害。同时,还提高细胞组织器官的代谢能力、提高人体免疫力。(The invention relates to quaternary ammonium salt ozone oil and a preparation method thereof. The preparation raw materials of the quaternary ammonium salt ozone oil comprise chitosan quaternary ammonium salt, olive oil and ozone; the dosage of the chitosan quaternary ammonium salt is 2g to 5g corresponding to every 500mL of the olive oil, and the dosage of the ozone is 12g to 25g corresponding to every 500mL of the olive oil. According to the invention, a specific sterilization and disinfection formula is formed by matching and using a proper amount of olive oil, ozone and chitosan quaternary ammonium salt, and when the formula is used for sterilizing and disinfecting wound tissues, the three components are synergistic, so that the sterilization and disinfection effect and the oxidation capacity are very strong. Furthermore, the inventors have found that the formulation does not cause damage to healthy tissue surrounding the wounded tissue during oral administration. Meanwhile, the metabolism capability of cell tissues and organs is improved, and the immunity of the human body is improved.)

1. The quaternary ammonium salt ozone oil is characterized in that raw materials for preparing the quaternary ammonium salt ozone oil comprise chitosan quaternary ammonium salt, olive oil and ozone; the dosage of the chitosan quaternary ammonium salt is 2g to 5g corresponding to every 500mL of the olive oil, and the dosage of the ozone is 12g to 25g corresponding to every 500mL of the olive oil.

2. The quaternary ammonium salt ozone oil of claim 1, wherein the amount of the chitosan quaternary ammonium salt is 2g to 3g and the amount of the ozone is 22g to 24g per 500mL of the olive oil.

3. The quaternary ammonium salt ozone oil of claim 2, wherein the amount of the chitosan quaternary ammonium salt is 2.5g to 2.8g and the amount of the ozone is 22.4g to 23.7g per 500mL of the olive oil.

4. The quaternary ammonium salt ozone oil of claim 1, wherein the amount of the chitosan quaternary ammonium salt is 4.3g to 4.8g and the amount of the ozone is 12g to 16g per 500mL of the olive oil.

5. The quaternary ammonium salt ozone oil of claim 4, wherein the amount of the chitosan quaternary ammonium salt is 4.5g to 4.7g and the amount of the ozone is 14g to 15g per 500mL of the olive oil.

6. The quaternary ammonium salt ozone oil of claim 1, wherein the amount of the chitosan quaternary ammonium salt is 3.5g to 4g and the amount of the ozone is 18.5g to 20g per 500mL of the olive oil.

7. The quaternary ammonium salt ozone oil of claim 6, wherein the amount of the chitosan quaternary ammonium salt is 3.6g to 3.9g and the amount of the ozone is 19g to 19.5g per 500mL of the olive oil.

8. The quaternary ammonium salt ozone oil according to any one of claims 1 to 7, wherein the olive oil has a purity of 99% or more; or/and the purity of the chitosan quaternary ammonium salt is more than 99%.

9. The method of any of claims 1 to 8, wherein the method comprises: adding the chitosan quaternary ammonium salt into the olive oil, and introducing the ozone into the obtained mixture after stirring.

10. The method of claim 9, wherein the ozone is diluted with air to a final concentration of 60-100mg/mL and introduced into the mixture; or/and the quaternary ammonium salt ozone oil is packaged by a plastic bag or a plastic bottle; or/and the quaternary ammonium salt ozone oil is packaged by a glass bottle.

Technical Field

The invention relates to the technical field of medicines, in particular to quaternary ammonium salt ozone oil and a preparation method thereof.

Background

Currently, the sterilization and disinfection formulations commonly used in medicine are, for example: 70-90 parts of natural olive oil, 0.5-5 parts of negative oxygen ions, 1-4 parts of hyaluronic acid, 5-20 parts of rose oil, 1-5 parts of borneol, 2-10 parts of chitosan quaternary ammonium salt, 0.5-3 parts of radix sophorae flavescentis extract, 0.5-3 parts of scutellaria baicalensis extract, 1-5 parts of aloe extract and 5-20 parts of deionized water. For another example: 0.5-3 parts of medical ozone, 70-90 parts of soybean oil, 0.5-2.5 parts of peppermint oil, 10-20 parts of olive oil and 0.1-0.8 part of matrine. However, these conventional formulations have not been effective when sterilized by human disinfection or orally administered to human body.

Disclosure of Invention

Based on the above, the main purpose of the invention is to provide quaternary ammonium salt ozone oil, and the effect is very obvious when the quaternary ammonium salt ozone oil is used for sterilizing and disinfecting human wound tissues.

The main purpose of the invention is realized by the following technical scheme:

the preparation raw materials of the quaternary ammonium salt ozone oil comprise chitosan quaternary ammonium salt, olive oil and ozone; the dosage of the chitosan quaternary ammonium salt is 2g to 5g corresponding to every 500mL of the olive oil, and the dosage of the ozone is 12g to 25g corresponding to every 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 2g to 3g and the amount of the ozone is 22g to 24g per 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 2.5g to 2.8g and the amount of the ozone is 22.4g to 23.7g per 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 4.3g to 4.8g and the amount of the ozone is 12g to 16g per 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 4.5g to 4.7g and the amount of the ozone is 14g to 15g per 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 3.5g to 4g and the amount of the ozone is 18.5g to 20g per 500mL of the olive oil.

In one embodiment, the amount of the chitosan quaternary ammonium salt is 3.6g to 3.9g and the amount of the ozone is 19g to 19.5g per 500mL of the olive oil.

In one embodiment, the olive oil has a purity of 99% or more; or/and the purity of the chitosan quaternary ammonium salt is more than 99%.

The preparation method of the quaternary ammonium salt ozone oil comprises the following steps: adding the chitosan quaternary ammonium salt into the olive oil, and introducing the ozone into the obtained mixture after stirring.

In one embodiment, the ozone is diluted with air to a final concentration of 60-100mg/mL before being introduced into the mixture.

In one embodiment, the quaternary ammonium salt ozone oil is packaged in an oxidation-resistant plastic bag.

In one embodiment, the quaternary ammonium salt ozone oil is packaged in a glass bottle.

Compared with the prior art, the invention has the following beneficial effects:

according to the invention, a specific sterilization and disinfection formula is formed by matching and using a proper amount of olive oil, ozone and chitosan quaternary ammonium salt, and when the formula is used for sterilizing and disinfecting wound tissues, the three components are synergistic, so that the sterilization and disinfection effect and the oxidation capacity are very strong. Furthermore, the inventors have found that the formulation does not cause damage to healthy tissue surrounding the wounded tissue during oral administration. Meanwhile, the metabolism capability of cell tissues and organs is improved, and the immunity of the human body is improved.

Detailed Description

In order that the invention may be more fully understood, reference will now be made to the following description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Chitosan quaternary ammonium salt: quaternary ammonium salt groups with large steric hindrance are introduced into chitosan molecules, and hydrogen bonding in the molecules and among the molecules is destroyed, so that the hydration capability of the molecules is enhanced, the water holding capacity of the molecules is increased, and the chitosan molecules have a very high moisture-preserving function; the quaternized chitosan and the surfactant have good intermiscibility, and the chitosan quaternary ammonium salt has good water solubility, can adjust moisture, and also has the properties of emulsification and foaming; the chitosan quaternary ammonium salt has good film forming property, can form a natural barrier on human skin, can effectively prevent and weaken the damage of ultraviolet rays and germs to the skin, and also has excellent antibacterial and bacteriostatic effects. The chitosan quaternary ammonium salt has safe, nontoxic and biocompatible properties; the chitosan quaternary ammonium salt is natural cationic polysaccharide, can be combined with DNA to become polymer electrolyte, and is an effective carrier in a gene delivery system; the chitosan quaternary ammonium salt also has remarkable antibacterial property.

Olive oil: the olive oil contains unsaturated fatty acid higher than any vegetable oil, abundant vitamin A, D, E, F, K, fat-soluble vitamins such as carotene, antioxidant, and cholesterol, and has high digestibility. The olive oil is rich in squalene with excellent affinity with skin and essential fatty acid of human body, is quickly absorbed, and effectively keeps the skin elastic and moist; the olive oil is rich in monounsaturated fatty acid, vitamin E, K, A, D, etc., and phenolic antioxidant substances, and has effects of eliminating facial wrinkle, preventing skin aging, caring skin and hair, and preventing hand and foot chap. More than 80% of monounsaturated fatty acid and omega-3 fatty acid are contained in the olive oil, the DHA in the omega-3 fatty acid can increase the sensitivity of insulin, and when the content of the unsaturated fatty acid in a cell membrane is higher, the number of the double bonds is more, and the mobility is stronger.

Ozone: (one) anti-inflammatory and anti-infective mechanism: ozone contacts body fluids to produce hydrogen peroxide, and ozone (O3) and hydrogen peroxide (H2O2) are two strong oxidants that, once inside the body, directly kill and eliminate pathogens such as bacteria, viruses and parasites, or cells that are diseased inside the body. Ozone can kill bacteria, fungi and viruses quickly and thoroughly. Mechanism of (di) oxidation of cholesterol: ozone generatorThe strong oxidizing property can break △ 5.6.6 double bonds of cholesterol to generate oxide nitrophenylhydrazone, which proves that ozone can oxidize cholesterol and has good effect on reducing blood fat, (III) pain relieving and pain relieving mechanisms: ① ozone can improve the activity of erythrocyte glutathione peroxidase and glucose 6-phosphate dehydrogenase and enhance lipid peroxidation reaction. ② ozone can stimulate the release of enkephalin and the like, and has the effect similar to chemical acupuncture and ③ ozone can oxidize and inactivate various pain-causing substances in vivo.ozone stimulates and enhances the immune system of the organism, probably the mechanism of ozone stimulation and enhances the immune system is that the ozone can react with unsaturated fatty acid on the surface of immune cells to generate lipid hydrogen peroxide chains, the lipid hydrogen peroxide chains enter cells, the nuclear factor NFKB can be activated, thereby activating the replication, transcription and translation of cellular mRNA, the synthesis of protein and the release of cytokines, (IV) stimulates and enhances the immune function of the organism, the ozone can activate immunocompetent cells, the release of cytokines, the release of interferons, the interleukin cytokines and the like is increased, the immune function of the organism is improved by the action of long-acting interferon, the ozone triphosphate can regulate the oxygen content of ozone, the basal oxygen-releasing of the basal oxygen-releasing system, the ATP can be increased, the metabolism of the oxidative metabolism of the organism, the metabolism of the cholesterol can be increased, the metabolism of human body, the metabolism of theAfter the ozone which is used for resisting inflammation and infection of the body surface decays and is reduced into oxygen, oxygen can be supplied to ulcer tissues, and the recovery of focus is promoted. (VI) ozone medical function foundation: medical ozone, which is a strong oxidizing agent second only to fluorine, has different effects on different compounds, has different reaction mechanisms and has obvious selectivity under different pH values. When the pH is less than or equal to 7.4, namely unsaturated fatty acid exists in physiological state, 1.3 dipolar substance of medical trioxane reacts with unsaturated fatty acid (such as phospholipid on cell membrane) to break double bond (especially isolated double bond) and generate peroxide. Physiological PH, whose ionic reaction mechanism dominates, does not promote the formation of free radicals. As a decomposition product of ozone (as opposed to the auto-oxidation of fatty acids by atmospheric oxygen), hydroperoxides enter the cell through the phospholipid space of the cell membrane, thereby affecting the metabolism of the cell, resulting in the elevation of ATP and 2, 3-DPG, and an increase in immunocompetent cells by activating the nuclear factor NFKB, producing an induction effect, and finally resulting in the release of cytokines. In an alkaline medium with pH not less than 8, medical ozone can generate free radical reaction, so that the reaction for forming hydroxyl radical is increased, and the free radical chain reaction is initiated. On the other hand, unlike oxygen, when medical ozone meets interstitial fluid, the following reactions occur immediately in plasma, lymph and urine: o is₃+ biological macromolecule->O₂After the above reaction between O-generating oxygen molecules and oxygen atoms, medical ozone molecules no longer exist. Medical ozone preferentially reacts with polyunsaturated fatty acids (PUFAs), antioxidants (e.g., ascorbic acid, uric acid, thiols), SH groups (e.g., cysteine, GSh (glutathione), and albumin). Ozone at different doses can also react with carbohydrates, enzymes, DNA, RNA, all of which are reduction reactions that lose electrons. The main reaction formula is as follows: R-CH ═ CH-R' + O₃＝H₂O->R-CH＝O+R’-CH＝O+H₂O₂。

The embodiment of the invention provides quaternary ammonium salt ozone oil, which is prepared from raw materials including chitosan quaternary ammonium salt, olive oil and ozone; the dosage of the chitosan quaternary ammonium salt is 2g to 5g corresponding to every 500mL of the olive oil, and the dosage of the ozone is 12g to 25g corresponding to every 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 2g to 3g and the amount of the ozone is 22g to 24g per 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 2.5g to 2.8g and the amount of the ozone is 22.4g to 23.7g per 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 4.3g to 4.8g and the amount of the ozone is 12g to 16g per 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 4.5g to 4.7g and the amount of the ozone is 14g to 15g per 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 3.5g to 4g and the amount of the ozone is 18.5g to 20g per 500mL of the olive oil.

Preferably, the amount of the chitosan quaternary ammonium salt is 3.6g to 3.9g and the amount of the ozone is 19g to 19.5g per 500mL of the olive oil.

Preferably, the purity of the olive oil is above 99%, and the purity of the chitosan quaternary ammonium salt is above 99%.

The quaternary ammonium salt ozone oil provided by the embodiment of the invention can be prepared into a dressing paste, and is suitable for treating various traumas and skin diseases; can also be prepared into oral products, and is suitable for treating various internal diseases and conditioning sub-health. Has obvious effect and wide clinical application range.

The embodiment of the invention also provides a preparation method of the quaternary ammonium salt ozone oil, which comprises the following steps: adding the chitosan quaternary ammonium salt into the olive oil, and introducing the ozone into the obtained mixture after stirring.

Preferably, the ozone is diluted by air to a gas with a final concentration of 60-100mg/mL and then introduced into the mixture.

Preferably, the quaternary ammonium salt ozone oil is packaged with an oxidation resistant plastic bag.

Preferably, the quaternary ammonium salt ozone oil is packaged by a glass bottle.

The chitosan quaternary ammonium salt of the embodiment of the invention is derived from a commercially available product, and the olive oil can be commercially available or can be obtained by extracting and refining by a conventional method.