阅读说明：本技术 一种蠕虫状昆虫幼虫标本的制作方法及其应用 (Method for preparing vermiform insect larva specimen and application thereof ) 是由 胡霞 初旭 马秋雨 陈昊 罗明燕 吴红敏 张飞萍 梁光红 王荣 吴松青 于 2019-11-29 设计创作，主要内容包括：本发明涉及生物标本制作技术领域,具体涉及一种蠕虫状昆虫幼虫标本的制作方法及其应用,该昆虫幼虫标本制作方法应用于昆虫幼虫标本展示、保存；通过物理手法将蠕虫状昆虫幼虫内容物去除,低龄幼虫填入无色明胶,高龄幼虫填入Elmer’s无色水晶泥,将处理后的昆虫幼虫进行风干处理,昆虫风干后将昆虫转移至标本盒中并通过昆虫针固定,最后进行防腐防潮处理；该昆虫幼虫标本制作方法将有效地对蠕虫状昆虫幼虫进行保存和展示,弥补了蠕虫状昆虫幼虫标本的空缺,对昆虫教学时间以及昆虫生活史的探索具有重要意义；另外昆虫幼虫填充材料为水晶泥和明胶,具有易操作,还原度高,成本低,环保,方便运输,与传统昆虫标本不同,更加实用于教学实践中。(The invention relates to the technical field of biological specimen preparation, in particular to a preparation method and application of a worm-shaped insect larva specimen, wherein the preparation method of the insect larva specimen is applied to the display and the preservation of the insect larva specimen; removing the content of vermicular insect larvae by a physical method, filling colorless gelatin into the low-age larvae, filling Elmer's colorless crystal mud into the high-age larvae, carrying out air drying treatment on the treated insect larvae, transferring the insects into a specimen box after the insects are dried by air, fixing the insects by an insect needle, and finally carrying out antiseptic and dampproof treatment; the insect larva specimen preparation method can effectively store and display the vermicular insect larvae, makes up the vacancy of the vermicular insect larva specimen, and has important significance for the exploration of insect teaching time and insect life history; in addition, the insect larva filling material is crystal mud and gelatin, is easy to operate, high in reduction degree, low in cost, environment-friendly and convenient to transport, is different from traditional insect specimens, and is more practical in teaching practice.)

1. A method for preparing a vermicular insect larva specimen is characterized in that auxiliary tools and colorless filling colloids are used for replacing the contents of vermicular insect larvae, and the processed vermicular insect larvae are stored in a specimen box.

2. The method of claim 1, comprising the steps of:

s1: physically removing the contents in the body of the vermiform insect larvae;

s2: injecting colorless filling colloid into the body of the vermiform insect larva by using an injector and sealing the wound;

s3: air-drying the insect larvae treated by the S2;

s4: the insect larvae after being air-dried are fixed in the specimen box through the insect needle, and the specimen is subjected to antiseptic and damp-proof treatment after the insects are fixed.

3. The method of claim 2, wherein the step of S1 is as follows:

s1.1: putting the worm-shaped insect larvae into water at 70 ℃ and boiling for 1-2 minutes;

s1.2: using a scalpel to cut a middle body segment on one side of the abdomen of the vermiform insect larva in a direction perpendicular to the direction of the larva body;

s1.3: the contents of the vermiform insect larvae were removed using forceps, a scalpel, and a syringe.

4. The method of claim 3, wherein the tweezers comprise straight tweezers and curved-tip tweezers.

5. The method of claim 3, wherein the step S2 comprises the following steps:

s2.1: injecting colorless filling colloid into the insect body along the incision of the middle body segment of the worm-shaped insect larva by using an injector so that all corners of the insect body are filled with the colloid;

s2.2: the dissected wound is sealed with glue.

6. A method of preparing a worm-like insect larva specimen according to claim 2 or 5,

if the worm-shaped insect larvae are of low-age larvae, the injected colorless filling colloid is colorless gelatin;

if the worm-shaped insect larvae are of high age, the colorless filling colloid is Elmer's colorless crystal mud.

7. The method of claim 2, wherein the cover of the box in S4 is made of transparent material.

8. Use of a method of making a worm-like insect larvae sample according to any one of claims 1 to 7 for the display and preservation of worm-like insect larvae samples.

Technical Field

The invention relates to the technical field of biological specimen preparation, in particular to a preparation method and application of a vermicular insect larva specimen.

Background

Insects are closely related to the life of human beings, and the known insects are 100 thousands of insects. In order to make people more convenient to research, study and appreciate insects, the insects are made into insect specimens so as to be stored for a long time, and meanwhile, the insect specimens are important basis for determining insect species and are fresh and alive teaching materials for knowing the insects. In addition, for the whole life history of the insect, people are required to know the habits and the forms of the adult insect and the living states of the insect larvae, so that worm-shaped insect larva specimens are prepared, and precious data are left for subsequent scientific research. For people to study, study and appreciate.

The existing insect specimen manufacturing and displaying method is manufactured based on adult insects, few specimens of insect larvae exist, even larva specimens are basically preserved in alcohol, and the larva specimens are soaked in the alcohol for a long time to cause the larva to decolor and deform, so that the original morphological characteristics are lost, and people can not clearly know the life history of the insects.

In addition, in the process of manufacturing insect specimens, most of the insect specimens are only roughly spread wings, adjusted in posture and air-dried, and the insects are not carefully dissected and preserved, so that the specimens cannot be stored for a long time, and a great deal of waste of resources is caused.

Through the analysis, the insect specimen preparation method in the prior art has a great deal of resource waste and larva requirements.

Disclosure of Invention

The invention provides a method for preparing a vermiform insect larva specimen, which can effectively solve the problems.

The invention is realized by the following technical scheme:

a method for preparing the specimen of worm-shaped insect larva features that the auxiliary tool and colorless filler colloid are used to replace the content of worm-shaped insect larva, and the treated worm-shaped insect larva is stored in specimen box.

The method specifically comprises the following steps:

s1: physically removing the contents in the body of the vermiform insect larvae;

s2: injecting colorless filling colloid into the body of the vermiform insect larva by using an injector and sealing the wound;

s3: air-drying the insect larvae treated by the S2;

s4: the insect larvae after being air-dried are fixed in the specimen box through the insect needle, and the specimen is subjected to antiseptic and damp-proof treatment after the insects are fixed.

Further, the specific steps of S1 are as follows:

s1.1: putting the worm-shaped insect larvae into water at 70 ℃ and boiling for 1-2 minutes;

s1.2: using a scalpel to cut a middle body segment on one side of the abdomen of the vermiform insect larva in a direction perpendicular to the direction of the larva body;

s1.3: the contents of the vermiform insect larvae were removed using forceps, a scalpel, and a syringe.

Wherein, the tweezers comprise straight tweezers and curved-mouth tweezers.

Further, the specific steps of S2 are as follows:

s2.1: injecting colorless filling colloid into the insect body along the incision of the middle body segment of the worm-shaped insect larva by using an injector so that all corners of the insect body are filled with the colloid;

s2.2: the dissected wound is sealed with glue.

Further, if the worm-shaped insect larvae are low-age larvae, the injected colorless filling colloid is colorless gelatin;

if the worm-shaped insect larvae are of high age, the colorless filling colloid is Elmer's colorless crystal mud.

Furthermore, the cover of the notebook computer in S4 is made of transparent material.

The method for manufacturing the vermicular insect larva specimen is applied to displaying and storing the vermicular insect larva specimen.

Has the advantages that:

the invention provides a method for manufacturing a vermicular insect larva specimen, which effectively stores and displays vermicular insect larvae, makes up for the vacancy of the vermicular insect larva specimen and has important significance for the exploration of insect teaching time and insect life history. In addition, the insect larva filling material is crystal mud and gelatin, is easy to operate, high in reduction degree, low in cost, environment-friendly and convenient to transport, is different from traditional insect specimens, and is more suitable for teaching practice.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A method for preparing the specimen of worm-shaped insect larva features that the auxiliary tool and colorless filler colloid are used to replace the content of worm-shaped insect larva, and the treated worm-shaped insect larva is stored in specimen box.

The method specifically comprises the following steps:

s1: physically removing the contents in the body of the vermiform insect larvae;

the method comprises the following specific steps:

s1.1: putting the worm-shaped insect larvae into water at 70 ℃ and boiling for 1-2 minutes;

s1.2: using a scalpel to cut a middle body segment on one side of the abdomen of the vermiform insect larva in a direction perpendicular to the direction of the larva body;

s1.3: the contents of the vermiform insect larvae were removed using forceps, a scalpel, and a syringe.

Wherein, in the process of removing the contents in the body of the vermiform insect larva, the residual contents gathered on the insect epidermis need to be washed clean by clear water.

S2: injecting colorless filling colloid into the body of the vermiform insect larva by using an injector and sealing the wound;

the method comprises the following specific steps:

s2.1: injecting colorless filling colloid into the insect body along the incision of the middle body segment of the worm-shaped insect larva by using an injector so that all corners of the insect body are filled with the colloid;

further, if the worm-shaped insect larvae are low-age larvae, the injected colorless filling colloid is colorless gelatin; if the worm-shaped insect larvae are of high age, the colorless filling colloid is Elmer's colorless crystal mud.

S2.2: the dissected wound is sealed with glue.

S3: air-drying the insect larvae treated by the S2;

wherein, insect larva air-dries the processing mode and not only can adopt natural air-dry or freezing air-dry, can also adopt other air-dries processing modes, as long as can make insect larva reach dry dehydration, specifically adopts which kind of air-dries the processing mode and can select according to actual need.

S4: the insect larvae after being air-dried are fixed in the specimen box through the insect needle, and the specimen is subjected to antiseptic and damp-proof treatment after the insects are fixed.

Wherein, the antiseptic and damp-proof treatment of the specimen is realized by adding an antiseptic and a desiccant.

Furthermore, the cover of the specimen box in S4 can be made of transparent material such as transparent plastic and glass, so as to facilitate the specimen display.

The invention provides a method for making and displaying vermiform insect larva specimens, which is applied to the display and preservation of insect larva specimens. Removing the content of vermiform insect larvae by a physical method, filling colorless gelatin into the low-age larvae, filling Elmer's colorless crystal mud into the high-age larvae, carrying out air drying treatment on the treated insect larvae, transferring the insects into a specimen box after the insects are dried by air, fixing the insects by an insect needle, and finally adding a preservative and a drying agent.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.