阅读说明：本技术 一种含二氢卟吩铁的植物生长调节组合物及其应用 (Plant growth regulating composition containing chlorin iron and application thereof ) 是由 陈黎明 王乐天 郭素春 任勇 王迎春 于 2019-11-04 设计创作，主要内容包括：本发明提供一种含二氢卟吩铁的植物生长调节组合物及其应用,其包括第一活性组分二氢卟吩铁,第二活性组分调环酸钙或萘乙酸,所述二氢卟吩铁与调环酸钙的质量比为(0.01～0.5):(10～30),所述二氢卟吩铁与萘乙酸的质量比为(0.01～0.5):(5～10)。本发明给出了所述组合物的制备剂型为可溶性粉剂、水分散粒剂、可湿性粉剂或可溶性液剂。所述组合物用于促进番茄、水稻生长以及改善番茄品质。本发明拓展了二氢卟吩铁在植物生长调节剂领域的应用。(The invention provides a plant growth regulating composition containing chlorin iron and an application thereof, wherein the plant growth regulating composition comprises a first active component chlorin iron and a second active component prohexadione calcium or naphthylacetic acid, the mass ratio of the chlorin iron to the prohexadione calcium is (0.01-0.5): 10-30), and the mass ratio of the chlorin iron to the naphthylacetic acid is (0.01-0.5): 5-10. The preparation formulation of the composition provided by the invention is soluble powder, water dispersible granules, wettable powder or soluble liquid. The composition is used for promoting tomato and rice growth and improving tomato quality. The invention expands the application of chlorin iron in the field of plant growth regulators.)

1. A plant growth regulating composition containing chlorin iron is characterized by comprising a first active component chlorin iron and a second active component of prohexadione calcium or naphthylacetic acid, wherein the mass ratio of the chlorin iron to the prohexadione calcium is (0.01-0.5) - (10-30), and the mass ratio of the chlorin iron to the naphthylacetic acid is (0.01-0.5) - (5-10).

2. The chlorin iron-containing plant growth regulating composition as claimed in claim 1, wherein the composition is prepared in the form of soluble powder, water dispersible granule, wettable powder, soluble liquid.

3. Use of a chlorin iron-containing plant growth regulating composition according to claim 1 for improving plant growth and development.

4. The use according to claim 3, wherein the composition is formulated for use as a solution, wherein the concentration of chlorin iron is 0.01-0.5 mg/L and the concentration of prohexadione calcium is 10-30 mg/L.

5. The use according to claim 3, wherein the composition is formulated for use as a solution, wherein the concentration of chlorin iron is 0.01-0.5 mg/L and the concentration of naphthalene acetic acid is 5-10 mg/L.

6. The use according to claim 3, wherein the composition is for promoting the growth of rice.

7. Use according to claim 3, wherein the composition is for promoting tomato growth and improving tomato quality.

Technical Field

The invention belongs to the technical field of agricultural compositions, relates to a plant growth regulating composition, and particularly relates to a plant growth regulating composition containing chlorin iron. The invention also provides the application of the composition as a plant growth regulator.

Background

In the process of plant growth, trace physiological active substances play an important role in the growth of plants like nutrients such as sunlight, temperature, water and the like, and the trace physiological active substances have a special role in regulating the growth and development of the plants. Various endogenous trace active substances are often referred to as plant hormones, such as auxins, gibberellins, cytokinins, etc., while non-endogenous additional active substances are referred to as plant growth regulators.

Although the endogenous growth regulating active substances of plants show strong activity at extremely low concentration (< 1 mu mol/L), the content of the endogenous growth regulating active substances is extremely low, the endogenous growth regulating active substances are difficult to extract and prepare, and the price is high, so that the endogenous growth regulating substances of non-plants become important points for research.

Plant growth regulators include two major types of natural extraction and artificial synthesis, at present, more than one hundred products are put into market for application, and the plant growth regulators become an important technical method for improving the yield and quality of agricultural products (Fuhualong and the like, research and application of plant growth regulators, and bioprocessing, 2008(6)4: 7-12). Plant growth regulators generally have the characteristics of promoting plant growth at a low concentration and inhibiting plant growth at a high concentration, and can exert normal efficacy only by using the plant growth regulators in a narrow dosage range, which causes various limitations and inconveniences in practical application; compared with the prior art, the prior exogenetic synthetic plant growth regulator has higher dosage, larger concentration, unclear metabolic pathway, stable chemical property, more residues or long residual effect period, and the residue problem has aroused high attention of people along with the continuous expansion of the application range (Zhao Min and the like, the safety of the plant growth regulator on crops and environment, journal of environment and health, 2007(24)5: 370-372), China has stipulated that any green food production and processing process can not use the chemically synthesized plant growth regulator in the form of fertilizer and pesticide (the agricultural industry standard NY-T393-2000 green food pesticide use rule of China's republic of China; NY-T394-2000 green food fertilizer use rule). The plant growth regulator with small research and development dosage, stable property and convenient use has become the development trend in the field, and the high-efficiency safe product which has no toxicity or side effect and does not influence the normal metabolic pathway and mode of the plant is the key difficulty for researching the plant growth regulator.

The chlorin iron has excellent plant growth regulating activity and obvious in-vivo and in-vitro chlorophyllase inhibiting effect. The chemical structure of chlorin iron and its application as plant growth regulating active component have been described in detail in the patent application CN102285992B by the present applicant. The chlorin iron of the present patent application is the chlorin iron of patent CN 102285992B.

Prohexadionexcium, english name, chemical name: 3, 5-dichloro-4-propionylcyclohexanecarboxylic acid and calcium salts. Prohexadione calcium is a novel plant growth regulator, and has the effects of promoting reproductive growth, inhibiting growth of overground part vegetative organs, increasing lodging resistance and the like by being applied to crops such as rice, wheat, corn, barley and the like. The prohexadione calcium is a gibberellin biosynthesis inhibitor, inhibits the vigorous growth of crops by reducing the content of gibberellin in plants, and is low in toxicity and safe. At present, prohexadione calcium is mainly applied to gramineous crops, beans, lawns and fruit trees for controlling vigorous growth.

The naphthylacetic acid, also called α -naphthylacetic acid, English name 1-naphthylacetic acid, chemical name 2- (1-naphthyl) acetic acid, naphthylacetic acid is a kind of auxin, also is a broad-spectrum plant growth regulator, it has the action characteristics and physiological functions of endogenous auxin indoleacetic acid, such as promoting cell division and enlargement, inducing to form adventitious root, increasing fruit setting, preventing fruit drop, changing the ratio of female and male flowers, etc.

Disclosure of Invention

The technical problems to be solved by the invention are as follows: the application research of the chlorin iron serving as a plant growth regulating active ingredient with a novel action mechanism mainly stays in the experimental research stage. Based on the above, in order to promote the application of the chlorin iron in the field of agriculture and forestry, and by combining the early test verification data, the chlorin iron and the existing plant growth regulator are compounded to provide the chlorin iron-containing plant growth regulating composition.

In order to achieve the purpose, the invention adopts the following technical scheme:

a plant growth regulating composition containing chlorin iron is characterized by comprising a first active component chlorin iron and a second active component of prohexadione calcium or naphthylacetic acid, wherein the mass ratio of the chlorin iron to the prohexadione calcium is (0.01-0.5) - (10-30), and the mass ratio of the chlorin iron to the naphthylacetic acid is (0.01-0.5) - (5-10).

The plant growth regulating composition containing the chlorin iron can be prepared into any dosage form convenient to apply in the field of agriculture and forestry. In consideration of the physicochemical properties of chlorin iron, prohexadione calcium and naphthylacetic acid and the convenience of using corresponding preparation formulations, the preparation formulations of the composition are preferably soluble powder, water dispersible granules, wettable powder and soluble liquid.

In the preparation of soluble solutions, those skilled in the art are familiar with the use of corresponding adjuvants to complete the present invention, and the adjuvants selected can be exemplified by: emulsifier such as one or more of calcium dodecylbenzene sulfonate (Nongru 500#), Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru 2201#, span-60 # (common name: sorbitan stearate), Tween-60 # (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Nongru 1601# (common name: triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), Nongru 600#, and Nongru 400 #; solvent such as one or more of methanol, dimethylformamide, N-methylpyrrolidone, cyclohexanone, acetone, and dimethyl sulfoxide.

When preparing water dispersible granules, the auxiliaries selected by those skilled in the art can be exemplified by: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrating agent such as one or more of ammonium sulfate, urea, sucrose, and glucose; binders such as one or more of diatomaceous earth, corn starch, PVA, carboxymethyl (ethyl) cellulose; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite and pottery clay.

When preparing wettable powders or soluble powders, the adjuvants selected by the person skilled in the art may be exemplified by: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; the filler is one or more of ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite and pottery clay.

In another aspect, the present invention also provides the use of said chlorin iron-containing plant growth regulating composition for improving plant growth and development.

Preferably, the composition is prepared into a solution for use, and in the solution, the concentration of the chlorin iron is 0.01-0.5 mg/L, and the concentration of the prohexadione calcium is 10-30 mg/L.

Preferably, the composition is prepared into a solution for use, and the concentration of the chlorin iron in the solution is 0.01-0.5 mg/L, and the concentration of the naphthylacetic acid in the solution is 5-10 mg/L.

The plant growth regulating composition containing chlorin iron has at least the following beneficial effects or advantages:

1) the chlorin iron is green and environment-friendly, is easy to be decomposed and metabolized by plants, has no toxicity to human and livestock, has low dosage, selects plant growth regulating active ingredients such as prohexadione calcium or naphthylacetic acid and the like as the chlorin iron compound composition, obtains test data that the chlorin iron has a synergistic effect, and expands the application of the chlorin iron in the field of plant growth regulators.

2) Through a large number of deep theories and experimental researches, the inventor discovers that the plant growth regulating composition containing chlorin iron has strong activity, good effect and wide action performance, can improve the tillering number, the grain number and the thousand-grain weight of rice, improve the fruiting number and the fruit weight of a single tomato plant and improve the tomato quality.

Detailed Description

In order to facilitate understanding of the objects, technical solutions and effects of the present invention, the present invention will be further described in detail with reference to examples.

Preparation example

Formulation example 1

The formula of the 5% dihydroporphin iron prohexadione calcium soluble liquid comprises the following components: 0.02% of chlorin iron, 4.98% of prohexadione calcium, 2% of ammonium sulfate, 4% of calcium dodecyl benzene sulfonate, 3% of TX-10, 4% of N-methyl pyrrolidone and deionized water to make up to 100%. The materials are uniformly mixed in a mixing and stirring kettle according to the corresponding proportion, and the soluble solution of chlorin iron prohexadione calcium with the content of the effective component of 5 percent is prepared.

Formulation example 2

1.5% chlorin iron naphthalene acetic acid soluble liquor formula composition: 0.5% of chlorin iron, 1% of naphthylacetic acid, 2% of dimethyl sulfoxide, 2% of span-60 #, 5% of TX-10, 4% of methanol and deionized water to make up to 100%. The materials are evenly mixed in a mixing and stirring kettle according to the corresponding proportion, and the dihydroporphin iron-naphthylacetic acid soluble liquid with the content of the active ingredients of 1.5 percent is prepared.

Formulation example 3

The formula of 0.8% chlorin iron-naphthylacetic acid soluble powder comprises: 0.4% of chlorin iron, 0.4% of naphthylacetic acid, 5% of N-methyl pyrrolidone, 5% of glucose, 6% of calcium dodecyl benzene sulfonate and ammonium sulfate are supplemented to 100%. The materials are mixed and crushed evenly according to the corresponding proportion, and the chlorin iron-naphthylacetic acid soluble powder with the content of the effective component of 0.8 percent is prepared.

Formulation example 4

The 3% chlorin iron prohexadione calcium wettable powder comprises the following components in percentage by weight: 0.08 percent of chlorin iron, 2.92 percent of prohexadione calcium, 5 percent of glucose, 4 percent of lignosulfonate, 2 percent of urea, 3 percent of white carbon black and kaolin which are supplemented to 100 percent. The materials are put into a double-helix stirrer according to a corresponding proportion, are uniformly mixed and then are crushed by a jet mill, and the chlorin iron prohexadione calcium wettable powder with the effective component content of 3 percent is prepared.

(II) examples of drug efficacy test

Drug effect example 1

This example shows the rice growth promotion test of said chlorin iron-containing plant growth regulating composition. The rice variety used for the test material is Shanyou 016. Seeding is carried out 7 months and 3 days in 2019, spraying is carried out on the seedlings in a leaf-by-leaf and heart period, and 60kg of pesticide is sprayed per mu. Draining the seedling field water one day before spraying, and rehydrating one day after spraying. The tillering number of a single plant is investigated one day before transplanting, and the number of grains per ear, thousand grain weight and yield per mu are investigated when rice is mature. 30m per cell²The cells were randomized into blocks and the treatments sprayed with clear water were compared and repeated 4 times per treatment. Harvesting in the mature period and measuring the yield. The effect of the combination of chlorin iron and prohexadione calcium or naphthylacetic acid on rice tillering is shown in table 1. The effect of the combination of iron chlorins with prohexadione-calcium or naphthylacetic acid on rice yield is shown in Table 2.

TABLE 1 influence of the compounding of iron chlorin with prohexadione calcium or naphthylacetic acid on rice tillering

TABLE 2 influence of the combination of iron chlorins with calcium prohexadione or naphthylacetic acid on rice yield

The results in table 1 show that, compared with the clear water control, spraying of a chlorin iron single agent, a naphthylacetic acid single agent and a prohexadione calcium single agent can promote rice tillering to a certain extent under a set dosage; however, the dihydroporphin iron and the naphthylacetic acid or the prohexadione calcium are compounded for use, so that the tillering of rice is obviously improved. According to the morphological observation of field experiments, rice plants in the experimental group using the chlorin iron and naphthylacetic acid or prohexadione calcium in a compounding way grow more robustly and do not fall down.

The results of examining the influence of the application of the different plant growth regulators on the thousand seed weight and yield of rice are shown in Table 2. The results in table 2 show that different plant growth regulators applied at the set dose can improve the thousand kernel weight and yield of rice to different degrees, but the improvement effect is limited, compared with the clear water control; the compound use of the chlorin iron and the naphthylacetic acid or the prohexadione calcium has obvious increase on the thousand kernel weight and the yield of the rice, which shows that the rice thousand kernel weight and the yield are beneficial to storing nutrient substances in rice grains.

Drug effect example 2

This example shows the experiments on the growth and quality enhancement of tomatoes by the chlorin iron-containing plant growth regulating composition. The test material is tomato No. 4 vegetable. Planting medium-vegetable No. 4 tomato in sunlight greenhouse, planting 3600 plants per mu, and 20m per plot². The cultivation conditions of all the test districts are uniform and consistent and are consistent with local agricultural cultivation measures. Regulating growth and testing quality, and uniformly spraying the whole plant at leaf stage of 5-6 and early flowering stage according to each treatment concentration. The number of fruits harvested per cell per plant and the weight were recorded after tomato ripening and the average was calculated. And (3) collecting mature tomato fruits in the full bearing period to analyze the nutritional quality, wherein the analysis method is a common analysis method in the field, and the yield is measured.

The effects of the combination of chlorin iron and prohexadione calcium or naphthylacetic acid on the number of single tomato plants and the weight of single tomato plants are shown in Table 3, the effects on tomato solid, titratable acid and Vc are shown in Table 4, the effects on lycopene and soluble sugar are shown in Table 5, and the effects on tomato yield are shown in Table 6.

TABLE 3 Effect of the combination of iron chlorins with calcium prohexadione or naphthylacetic acid on the number of tomato plants and weight of individual fruits

TABLE 4 Effect of iron chlorins in combination with prohexadione-calcium or naphthylacetic acid on tomato solids, titratable acids and Vc

TABLE 5 Effect of iron chlorins in combination with prohexadione calcium or naphthylacetic acid on lycopene, soluble sugars

TABLE 6 influence of the combination of iron chlorins with calcium prohexadione or naphthylacetic acid on tomato yield

From the comprehensive tables 3-6, it can be seen that, compared with the clear water control, spraying of the chlorin iron single agent, the naphthylacetic acid single agent and the prohexadione calcium single agent can improve the quality and the yield of the tomatoes to a certain extent under the set dosage; however, the combination of the chlorin iron and the naphthylacetic acid or the prohexadione calcium has obvious improvement on the quality and the yield of the tomatoes, and obtains the synergistic effect.

The present invention has been further described with reference to the examples, but the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.