阅读说明：本技术 一种油包水体系丰胸多肽组合物 (Water-in-oil breast augmentation polypeptide composition ) 是由 丁文锋 彭晏 黄春青 于 2019-12-19 设计创作，主要内容包括：本发明公开一种油包水体系丰胸多肽组合物,属于美容多肽技术领域,所述油包水体系丰胸多肽组合物包含具有促进脂肪组织生长的多肽,还可以包含具有修复功能的多肽中的一种或几种,所述多肽存在于水相当中,各多肽质量百分浓度为0.0001％-5％,所述水相与油相形成油包水体系。所述油相包含表面活性剂、油性润肤剂。与水基体系相比,本发明所述丰胸多肽组合物采用油包水体系,多肽成分溶于少量水相,使得油包水体系中局部多肽浓度更高,更易于透皮吸收,在皮肤的蓄积量更高,同样的投料量可以达到更优异的丰胸效果,而且稳定性好、安全性高、制备工艺简单、成本低廉、适于工业化大生产。(The invention discloses a water-in-oil breast enhancement polypeptide composition, which belongs to the technical field of beauty polypeptide, and comprises polypeptide capable of promoting the growth of adipose tissues and one or more of polypeptide with a repair function, wherein the polypeptide exists in an aqueous phase, the mass percentage concentration of each polypeptide is 0.0001-5%, and the aqueous phase and an oil phase form a water-in-oil system. The oil phase comprises a surfactant and an oily emollient. Compared with a water-based system, the breast enlargement polypeptide composition adopts a water-in-oil system, polypeptide components are dissolved in a small amount of water phase, so that the concentration of local polypeptide in the water-in-oil system is higher, the transdermal absorption is easier, the accumulation amount on skin is higher, the same feeding amount can achieve a more excellent breast enlargement effect, and the breast enlargement polypeptide composition is good in stability, high in safety, simple in preparation process, low in cost and suitable for industrial mass production.)

1. The water-in-oil system breast enlargement polypeptide composition is characterized by comprising a polypeptide capable of promoting the growth of adipose tissues, wherein the polypeptide is present in an aqueous phase, the mass percentage concentration of the polypeptide is 0.0001-5%, and the aqueous phase and an oil phase form a water-in-oil system.

2. The water-in-oil system breast enhancing polypeptide composition of claim 1, wherein said polypeptide having the effect of enhancing adipose tissue growth is acetyl hexapeptide-38.

3. The water-in-oil system breast augmentation polypeptide composition of claim 1, wherein said polypeptide composition further comprises a repair polypeptide selected from one or more of the following polypeptides, including but not limited to: palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, tripeptide-1, tetrapeptide-21, palmitoyl hexapeptide-6, palmitoyl tripeptide-5, myristoyl pentapeptide-11, myristoyl hexapeptide-4, myristoyl octapeptide-1, hexapeptide-3, tripeptide-1 copper, hexapeptide-8, hexapeptide-9, hexapeptide-10, palmitoyl dipeptide-5 diaminobutyrylhydroxythreonine, palmitoyl dipeptide-6-diaminohydroxybutyric acid, palmitoyl hexapeptide-12, acetyl tetrapeptide-9, acetyl tetrapeptide-11 and tripeptide-10citrulline, wherein the mass percentage concentration of each polypeptide is 0.0001-5%.

4. The water-in-oil system breast enhancing polypeptide composition of claim 1, wherein said oil phase comprises a surfactant, an oily emollient.

5. The water-in-oil system breast enhancing polypeptide composition of claim 4, wherein the surfactant is one or more of PEG-20 triisostearin, PEG-7 glyceryl cocoate, PEG-6 caprylic/capric glycerides, polyglycerol-2 isostearate and polyglycerol-4 laurate, and the concentration of the surfactant is 5-20% by weight.

6. The water-in-oil system breast augmentation polypeptide composition of claim 4, wherein said oily emollient is one or a combination of hydrogenated polyisobutene, caprylic/capric triglyceride, isononyl isononanoate, and the concentration of said oily emollient is 70% to 90% by weight.

7. The water-in-oil system breast enhancing polypeptide composition of claim 6, wherein said oily emollient further comprises a vegetable oil, said vegetable oil is camellia seed oil, jojoba seed oil, nut oil, sunflower seed oil.

8. The water-in-oil system breast enhancing polypeptide composition of claim 1, wherein the water-in-oil system breast enhancing polypeptide composition is used primarily for preparing skin care products or medical products for external application to the skin.

Technical Field

The invention belongs to the technical field of beauty polypeptide, and relates to a water-in-oil breast augmentation polypeptide composition.

Background

With the improvement of living standard, people pay more and more attention to body beauty, for women, breast beauty is an important part of body beauty, and many women pay attention to their breasts and hope to have a firm and full chest. Many products are available on the market at present, and natural breast enlargement preparations are sought after by consumers due to the nature of the breast enlargement preparations.

The natural breast enlargement preparation is divided into sex hormones, phytoestrogens and products acting on adipose tissues. Among them, the breast augmentation preparation of sex hormone mainly promotes the growth of female breast through the combined action of estrogen or estrogen and progestogen, and the side effect is that the endometrial hyperplasia can be caused, and the risk of endometrial cancer and breast cancer is increased, so the addition of sex hormone in the cosmetics is forbidden. The phytoestrogen comprises three types of isoflavone, lignan and coumestrol, is a natural selective estrogen receptor modulator, has a bidirectional regulation effect, generally competes with endogenous estrogen at a lower dose to generate an antiestrogen effect, and can activate estrogen receptors which cannot be activated due to insufficient estrogen at a higher dose to generate an estrogen-like effect. Therefore, the phytoestrogen can cause great harm to human bodies when being used in excess. In addition, the phytoestrogens extraction process is complicated and costly, which limits their application. The substances acting on adipose tissue mainly include vitamin C, peptides, etc., and can promote differentiation of fat cells and fill adipose tissue of breast. The filling of adipose tissues is crucial to the smoothness, stiffness and uprightness of breasts, and the breast augmentation effect is difficult to achieve only by supplementing hormones without fat reserves. Among these substances acting on adipose tissues, polypeptides have been widely noticed by researchers because they have various physiological actions and are excellent in breast enhancement effect.

These active polypeptides are water soluble, and therefore most of the polypeptide breast enhancement products on the market are also water-based, in which the polypeptide dissolves. However, polypeptides are easily degraded in aqueous solution, and polypeptide components are directly added into water-based products, so that the stability of polypeptides in products is poor, the original polypeptides with high-efficiency activity cannot fully exert the due effect, and degradation products generated by degradation of polypeptides in products may possibly generate potential harm to human bodies. In addition, water-soluble substances are difficult to be absorbed through the skin barrier, and it is difficult to sufficiently exert their breast-enlarging effect. In order to increase the transdermal absorption and achieve the desired effect, the amount of the feed needs to be increased, which leads to an increase in cost.

Therefore, a breast enlarging product which has good stability, high safety, easy transdermal absorption, simple preparation process, low cost, suitability for industrial mass production, excellent effect and capability of overcoming the defects of the prior art is urgently needed in the field.

Disclosure of Invention

The invention aims to provide the breast enlarging polypeptide composition which has the advantages of good stability, high safety, easy transdermal absorption, simple preparation process, low cost, suitability for industrial mass production and excellent effect.

Through experimental research, the inventor finds that polypeptide components are dispersed in an oil phase to form a water-in-oil system, a small amount of water phase in the system can reduce the contact of the polypeptide and water, so that the polypeptide is concentrated in the water phase, the stability of the polypeptide is improved, and the polypeptide with a larger local concentration is favorable for exerting a better breast enhancement effect; the external oil phase can enable the polypeptide components to better penetrate through skin barriers and promote absorption, thereby endowing the product with more excellent breast enhancement effect, and forming the invention.

The invention provides a water-in-oil system breast augmentation polypeptide composition, which comprises a polypeptide capable of promoting the growth of adipose tissues, wherein the polypeptide exists in an aqueous phase, the mass percentage concentration of the polypeptide is 0.0001-5%, and the aqueous phase and an oil phase form a water-in-oil system.

The polypeptide capable of promoting the growth of the adipose tissues is acetyl hexapeptide-38.

The polypeptide composition may further comprise a polypeptide having a repair function selected from one or several of the following polypeptides, including but not limited to: palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, tripeptide-1, tetrapeptide-21, palmitoyl hexapeptide-6, palmitoyl tripeptide-5, myristoyl pentapeptide-11, myristoyl hexapeptide-4, myristoyl octapeptide-1, hexapeptide-3, tripeptide-1 copper, hexapeptide-8, hexapeptide-9, hexapeptide-10, palmitoyl dipeptide-5 diaminobutyrylhydroxythreonine, palmitoyl dipeptide-6-diaminohydroxybutyric acid, palmitoyl hexapeptide-12, acetyl tetrapeptide-9, acetyl tetrapeptide-11 and tripeptide-10citrulline, wherein the mass percentage concentration of each polypeptide is 0.0001-5%.

The oil phase comprises a surfactant and an oily emollient.

The surfactant is one or a combination of more of PEG-20 triisostearin, PEG-7 glyceryl cocoate, PEG-6 caprylic/capric glycerides, polyglycerol-2 isostearate and polyglycerol-4 laurate, and the mass percentage concentration of the surfactant is 5-20%.

The oily emollient is one or a combination of hydrogenated polyisobutene, caprylic/capric triglyceride and isononyl isononanoate, and the mass percentage concentration of the oily emollient is 70-90%.

The oily emollient may also comprise vegetable oil, which is camellia seed oil, jojoba seed oil, nut oil, sunflower seed oil.

The water-in-oil breast enlargement polypeptide composition is mainly used for preparing skin care products or medical products for external application of skin.

To facilitate understanding of the present invention, the breast enhancement principle and the mechanism of action of the polypeptides with different target sites are illustrated as follows:

1. principle of enlarging breast

Anatomically, the breast is located on the pectoralis major muscle, usually ranging from the second to the sixth rib, from the medial to the parasternal line, and from the lateral to the axillary line, and is composed mainly of glands, ducts, adipose tissue, fibrous tissue, etc., and has an internal structure like a "treelet" or "bunch of grapes" growing upside down. The fat tissue occupies most of the mammary gland and is distributed around the glandular lobes, between the glands and behind the muscles, so that the volume of the fat tissue determines the volume of the breast, and the volume of the fat tissue can be increased by increasing the number of fat cells or increasing the fat accumulation, thereby achieving the purpose of breast augmentation.

The formation of adipocytes is a process of transforming preadipocytes into mature adipocytes, a complex process involving gene expression and inhibition, which is successfully accomplished by requiring several essential factors to participate, more importantly, peroxisome proliferator-activated receptor gamma coactivator 1 α (PGC-1 α), which has a coactivating effect with a key receptor peroxisome proliferator-activated receptor gamma (PPAR γ). PPAR γ belongs to a member of the superfamily of nuclear receptors type II. PPAR γ is an important factor in adipose tissue, binds to a heterodimer with retinol X receptor bound to a target gene, regulates the expression of genes, and is important for the differentiation of preadipocytes.PGC-1 α enhances the expression of genes involved in adipocyte differentiation by coactivating with PPAR γ, thereby increasing the formation of fat.

Except for the fat tissue, the outside of the breast is covered by the skin, which plays the role of supporting the breast, so that the breast forms a hemisphere or a cone. The skin has a complex structure and is composed of epidermis, dermis and hypodermis, wherein the dermis is the main component of the skin and is composed of collagen, laminin, elastin, proteoglycan and the like. By maintaining the balance of collagen, laminin, elastin and proteoglycan in the dermis of the skin, the connective tissue of the skin can be reinforced, the elasticity and the firmness of the skin can be increased, and the breast can be better promoted to be firm.

2. Mechanism of action of polypeptides

2.1 the action mechanism of the polypeptide with the function of promoting the growth of adipose tissues

Acetyl Hexapeptide-38 (Acetyl Hexapeptide-38), through stimulating the expression of peroxisome proliferator-activated receptor gamma coactivator 1 α (PGC-1 α), PGC-1 α is coactivated with peroxisome proliferator-activated receptor gamma (PPAR gamma), promotes the growth of fat tissues in specific areas of the body including face and breast, increases the fat storage amount, increases the volume of specific areas, and leads to the increase of skin elasticity, the reduction of fine wrinkles and the filling of fat and water.

2.2 mechanism of action of the polypeptide with repair function

Palmitoyl Tripeptide-1 (Palmitoyl Tripeptide-1, Pal-GHK, CAS number: 147732-56-7) is modified by palmitic acid at the N-terminal of a peptide chain of GHK, has better transdermal performance, can promote skin to synthesize a large amount of collagen and polysaccharide, can tighten skin, lock water and moisturize skin, fade wrinkles and deeply repair skin.

Palmitoyl Tetrapeptide-7 (Palmitoyl Tetrapeptide-7, CAS number: 221227-05-0) helps promote collagen production and repair the thickened epidermal layer. In addition, palmitoyl tetrapeptide-7 may regulate the secretion of interleukin-6 (IL-6). IL-6 participates in chronic inflammatory reaction of skin, the increase of IL-6 can accelerate skin aging, and palmitoyl tetrapeptide-7 can reduce the level of IL-6 in the skin aging process, so that the balance of cell factors in the skin is maintained again, the skin inflammation is obviously eliminated, and glycosylation damage is reduced, thereby achieving the purposes of delaying skin aging, and increasing the skin elasticity and firmness.

Palmitoyl Pentapeptide-4 (Palmitoyl Pentapeptide-4) can stimulate the proliferation of fibroblasts and stimulate the synthesis of collagen, glycosaminoglycans (GAGs) and hyaluronic acid, thereby increasing the thickness of the skin and achieving the aim of repairing the skin.

Tripeptide-1 (GHK), an endogenous growth factor, acts as a transporter for copper ions, stimulating collagen synthesis, angiogenesis and fibroblast growth. The GHK with proper dosage has anti-inflammatory and repairing effects on skin. In addition, Marc Heidl et al found that tripeptide-1 could promote the synthesis of skin hyaluronic acid. Therefore, the tripeptide-1 can be used for repairing skin, replenishing water, moisturizing and improving skin quality.

Tetrapeptide-21 (GEKG) can promote the production of collagen in extracellular matrix (ECM), and induce the expression of collagen gene to increase, thereby thickening skin and improving the roughness of skin.

Palmitoyl Hexapeptide-6 (Palmitoyl Hexapeptide-6) is a peptide developed by taking genetic immune peptide as a template, and can effectively stimulate proliferation and linkage of fibroblasts and promote synthesis of collagen, so that dermis is repaired and skin quality is improved.

Palmitoyl Tripeptide-5 (Palmitoyl Tripeptide-5) stimulates fibroblast division and proliferation, strongly promotes the generation of collagen, supplements extracellular matrix proteins, strengthens connective tissues, inhibits the activity of matrix metalloproteinase, prevents collagen from decomposing, thereby making skin elastic and deeply repairing skin barrier by activating transforming growth factor- β (TGF- β).

Myristoyl Pentapeptide-11 (Myristoyl Pentapeptide-11) can stimulate collagen synthesis, help to restore skin elasticity again and repair damaged skin. The action mechanism is similar to that of Myristoyl hexapeptide-4 (Myristoyl hexapeptide-4) and Myristoyl Octapeptide-1 (Myristoyl Octapeptide-1).

Hexapeptide-3 (Hexapeptide-3) is a small molecule peptide similar to the 6-amino acid fragment sequence in the III-type unit of fibronectin molecule, promotes the expression of β 1 integrin, the adhesion of collagen, and the adhesion of cells and extracellular matrix (ECM), thereby promoting the remodeling of tissues and the repair of skin.

Tripeptide-1 Copper (Copper Peptide) can promote the generation of collagen, elastin, proteoglycan and glycosaminoglycan (GAGs) in large quantity, increase the elasticity of skin, tighten skin and smooth wrinkles; resisting oxidation, scavenging free radicals, and delaying skin aging; promoting epidermal cell proliferation, and repairing skin barrier.

Hexapeptide-8 (Hexapeptide-8), firms the skin by stimulating the production of laminin-V and type iv, vii collagen. Also similar in mechanism of action are Hexapeptide-10 (Hexapeptide-10), Palmitoyl Dipeptide-5 diaminobutyrylhydroxythreonine (palmityl Dipeptide-5 diaminobutyryl hydroxytrythronine) and Palmitoyl Dipeptide-6 diaminohydroxybutyric acid (palmityl Dipeptide-6 diaminohydroxybutyric acid).

Hexapeptide-9 (Hexapeptide-9) can increase the number of laminin-V and holomucin, increase the synthesis of collagen in the dermis, promote the regeneration and reinforcement of the structural tissue in the dermal-epidermal junction (DEJ) region, promote the differentiation of keratinocytes and the synthesis of keratin, thereby repairing wounds, recombining the complete epidermis, promoting the regeneration of the skin, enabling the skin to have more elasticity and reducing wrinkles.

Palmitoyl Hexapeptide-12 (Palmitoyl Hexapeptide-12, CAS number: 171263-26-6), a Spring fragment derived from elastin, can promote fibroblast proliferation and elastin synthesis, and improve skin elasticity. In addition, it can induce fibroblasts and monocytes to specific sites, promoting wound repair and tissue renewal.

Acetyl Tetrapeptide-9 (Acetyl Tetrapeptide-9) can promote synthesis of photoprotein glycan. Photoproteoglycans, a member of the family of small-molecule proteoglycans, are important components of the extracellular matrix (ECM), and can regulate collagen fibers to efficiently link them, thereby forming functionalized collagen fibers and maintaining skin thickness and firmness. Acetyl tetrapeptide-9 also promotes the synthesis of type I collagen, and deeply repairs the skin barrier by stimulating the synthesis of both proteoglycan and type I collagen.

Acetyl Tetrapeptide-11 (Acetyl Tetrapeptide-11), which stimulates the growth of keratinocytes, which further synthesize syndecan-1. syndecan-1 is a proteoglycan, and can strengthen the cohesion between epidermal cells. Acetyl tetrapeptide-11 also promotes collagen production from type XVII. Type XVII collagen is the major protein of hemidesmosome and plays an important role in the junction of epidermis and the epidermal-dermal junction. Acetyl tetrapeptide-11 remodels and reinforces the skin structure by stimulating the synthesis of collagen type syndecan-1 and XVII simultaneously.

Tripeptide-10Citrulline (CAS number: 960531-53-7) can compensate for the loss of function of core protein polysaccharide caused by aging, can be combined with collagen fibril to regulate the formation of collagen fibril, enhance the stability of the collagen fibril, ensure the uniform diameter and space structure of the fibril, maintain the integrity of the skin and make the skin soft and elastic.

The breast enlarging polypeptide composition of the water-in-oil system is obtained by combining the physiological structure characteristics of breasts, utilizing different action targets of the polypeptide on the skin and dispersing the polypeptide in the oil phase system. Compared with the prior art, the invention has the advantages that:

(1) the polypeptide is dissolved in a small amount of water phase and dispersed in the oil phase to form a water-in-oil system, so that the stability and the use safety of the polypeptide are improved.

(2) The polypeptide is concentrated in the water phase, so that the concentration of local active ingredients is increased, and the better breast enhancement effect is favorably achieved.

(3) The oil phase in the system can promote the transdermal absorption of the water-soluble polypeptide, and the same material amount can achieve better breast enhancement effect.

(4) The invention can achieve better breast enhancement effect with lower cost, has simple preparation process and is suitable for industrial mass production.

Drawings

FIG. 1 microscopic image (40X 10 times) of water-in-oil system of polypeptide composition

FIG. 2 in vitro cumulative transdermal and cumulative skin Retention of the polypeptide (24h)

Detailed Description

For a better understanding of the present invention, the following detailed description is given in conjunction with the following examples and drawings, but is not limited to the following examples.