阅读说明：本技术 一种基于电子信息技术的肺癌基因测序研究用分析仪器 (Lung cancer gene sequencing research analytical instrument based on electronic information technology ) 是由 周文静 于 2021-07-27 设计创作，主要内容包括：本发明公开了一种基于电子信息技术的肺癌基因测序研究用分析仪器,包括第二电机、第三电机、箱体、圆盘、第一电机、底板和放置槽,利用第二电机转动进而带动搅拌转轴上的搅拌棒转动,从而实现对箱体内测试样品试剂的搅拌混匀,打开阀门,使箱体内的测试样品试剂通过导液管进入输液筒内,利用第一电机转动进而带动丝杠转动,从而使第二移动块移动并进一步推动第一移动块移动,从而使输液筒内的测试样品试剂通过出液管后从喷头排出,利用第三电机转动进而带动筒体转动,进一步带动圆盘转动,从而使圆盘上不同位置上的放置槽转动至正对喷头,从而实现对测试样品试剂的持续和多次基因测序,检测效率高,同时保证了基因测序的准确性。(The invention discloses an analyzer for lung cancer gene sequencing research based on electronic information technology, which comprises a second motor, a third motor, a box body, a disc, a first motor, a bottom plate and a placing groove, wherein the second motor is used for rotating and further driving a stirring rod on a stirring rotating shaft to rotate, so that the reagent of a test sample in the box body is stirred and uniformly mixed, a valve is opened, the reagent of the test sample in the box body enters a liquid conveying cylinder through a liquid guide pipe, the first motor is used for rotating and further driving a lead screw to rotate, so that a second moving block moves and further pushes a first moving block to move, the reagent of the test sample in the liquid conveying cylinder is discharged from a spray head after passing through a liquid outlet pipe, the third motor is used for rotating and further driving the disc to rotate, so that the placing grooves on different positions on the disc are rotated to be opposite to the spray head, and continuous and multiple gene sequencing of the reagent of the test sample is realized, the detection efficiency is high, and meanwhile, the accuracy of gene sequencing is ensured.)

1. An analytical instrument for lung cancer gene sequencing research based on electronic information technology, includes bottom plate (5), its characterized in that: bottom plate (5) top has roof (2) through bracing piece fixed mounting, roof (2) top is provided with mixing device (1), mixing device (1) includes box (25), box (25) fixed mounting is at roof (2) top, the inlet of box (25) and the one end pipe connector of feed liquor pipe, the inside bilateral symmetry of box (25) is provided with first agitating unit (19) and second agitating unit (23), surface fixed mounting has the controller before box (25), the liquid outlet of box (25) passes through the catheter and is connected with the inlet of infusion section of thick bamboo (12), installs valve (14) on the catheter, infusion section of thick bamboo (12) are through bracing piece fixed mounting at bottom plate (5) top, the inside slip of infusion section of thick bamboo (12) is provided with first movable block (13), the inside position slip that is located first movable block (13) one side of infusion section of thick bamboo (12) is provided with second movable block (28) A lead screw (15) is transversely arranged in the liquid conveying cylinder (23), one end of the lead screw (15) extends out of the liquid conveying cylinder (12) and is fixedly connected with an output shaft of a first motor (3) through a coupler, the first motor (3) is fixedly arranged at the top of a supporting block (4), the supporting block (4) is fixedly arranged at the top of a bottom plate (5), the lead screw (15) transversely penetrates through a second moving block (28), a threaded through hole for being in threaded connection with the lead screw (15) is formed in the second moving block (28), one side of the second moving block (28) is fixedly connected with one side of a first moving block (13) through a push rod (29), a liquid outlet of the liquid conveying cylinder (12) is connected with one end of a liquid outlet pipe (11), the other end of the liquid outlet pipe (11) is connected with a spray head (10), a water outlet of the spray head (10) faces downwards, and a disc (8) is arranged under the spray head (10), the device is characterized in that a plurality of placing grooves (16) are formed in the position, corresponding to the spray head (10), of the top of the disc (8) at equal intervals along the circumferential direction, gene sequencing chips (17) are placed in the placing grooves (16), a barrel (6) is fixedly mounted at the top of the bottom plate (5), the bottom end of the barrel (6) is rotatably mounted at the top of the bottom plate (5) through a bearing seat, a rod body (7) is arranged in the barrel (6) in a sliding manner, the top end of the rod body (7) extends out of the barrel (6) and is fixedly connected to the bottom of the disc (8), the rod body (7) and the disc (8) are arranged concentrically, a threaded through hole for being in threaded connection with a bolt is formed in the outer side of the barrel (6), a belt pulley is sleeved on the outer side of the barrel (6), a third motor (26) is fixedly mounted at the top of the bottom plate (5), and an output shaft of the third motor (26) is fixedly connected with an input shaft of the speed reducer (27) through a shaft coupling, the belt pulley is installed on the output shaft of the speed reducer (27), and the belt pulley installed on the output shaft of the speed reducer (27) is connected with the belt pulley sleeved on the outer side of the barrel body (6) through a belt.

2. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1, wherein: second agitating unit (23) include stirring rod (21), second motor (22) and stirring pivot (24), stirring pivot (24) transversely set up in box (25), the output shaft fixed connection of shaft coupling and second motor (22) is passed through to the one end of stirring pivot (24), second motor (22) pass through bolt fixed mounting in the box (25) outside, install stirring rod (21) on stirring pivot (24).

3. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1 or 2, wherein: the second stirring device (23) and the first stirring device (19) are identical in structure and specifications of all parts.

4. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1, wherein: the heating device is characterized in that a heating pipe (20) is arranged between a first stirring device (19) and a second stirring device (23) inside the box body (25), the two ends of the heating pipe (20) extend out of the box body (25), the part of the heating pipe (20) located in the box body (25) is designed to be coiled, and the heating pipe (20) is made of glass.

5. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1, wherein: the outer side of the first moving block (13) and the inner wall of the infusion barrel (12) are both in polishing design and are tightly attached to each other.

6. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1, wherein: a clamping groove (18) is formed in the disc (8), the clamping groove (18) is an annular groove, a protective cover (9) is arranged on the disc (8), and the protective cover (9) is matched with the clamping groove (18).

7. The analyzer for lung cancer gene sequencing research based on electronic information technology according to claim 1, wherein: the first motor (3), the second motor (22) and the third motor (26) are respectively electrically connected with the controller through wires, and the controller is electrically connected with an external power supply through wires.

Technical Field

The invention relates to the technical field of gene sequencing, in particular to an analysis instrument for lung cancer gene sequencing research based on an electronic information technology.

Background

Lung cancer is one of the most rapidly growing malignancies that threaten human health and life. In many countries, the incidence and mortality of lung cancer have been reported to be significantly higher in recent 50 years, with lung cancer incidence and mortality in men accounting for the first of all malignancies, in women accounting for the second, and mortality accounting for the second. The etiology of lung cancer is not completely clear up to now, and lung cancer gene sequencing research is often required in the process of research on lung cancer treatment at present.

Gene sequencing is a novel gene detection technology, can analyze and determine the complete sequence of genes from blood or saliva, and predicts the possibility of suffering from various diseases, and the behavior characteristics and behaviors of individuals are reasonable. The gene sequencing technology can lock the individual pathological change gene and prevent and treat in advance. The gene sequencing technology is developed to the present, a corresponding gene sequencer is subjected to the change of covering the ground, the flux, the sequencing accuracy, the sequencing period, the reading length and the like are continuously promoted, the application is more and more extensive, the most key part of the gene sequencing is mainly completed by a gene sequencing chip, the commonly used technology is the NGS sequencing technology, and the sequence determination can be performed on hundreds of thousands to millions of DNA molecules at one time.

The existing lung cancer gene sequencing research analytical instrument based on the electronic information technology has the disadvantages of complex structure, inconvenient operation, incapability of realizing continuous and multiple gene sequencing of a test sample reagent and low detection efficiency.

Disclosure of Invention

The invention aims to provide an analyzer for lung cancer gene sequencing research based on an electronic information technology, so as to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

an analytical instrument for lung cancer gene sequencing research based on electronic information technology comprises a bottom plate, wherein a top plate is fixedly installed at the top of the bottom plate through a supporting rod, a blending device is arranged at the top of the top plate and comprises a box body, the box body is fixedly installed at the top of the top plate, a liquid inlet of the box body is connected with one end of a liquid inlet pipe in a pipe mode, a first stirring device and a second stirring device are symmetrically arranged on two sides inside the box body, a controller is fixedly installed on the front surface of the box body, a liquid outlet of the box body is connected with a liquid inlet of a liquid conveying cylinder through a liquid guide pipe, a valve is installed on the liquid guide pipe, the liquid conveying cylinder is fixedly installed at the top of the bottom plate through the supporting rod, a first moving block is slidably arranged inside the liquid conveying cylinder, a second moving block is slidably arranged at a position, which is located on one side of the first moving block, of the liquid conveying cylinder is transversely provided with a lead screw, one end of the lead screw extends out of the liquid conveying cylinder and is fixedly connected with an output shaft of a first motor through a coupler, the first motor is fixedly installed at the top of a supporting block, the supporting block is fixedly installed at the top of a bottom plate, the lead screw transversely penetrates through a second moving block, a threaded through hole for being in threaded connection with the lead screw is formed in the second moving block, one side of the second moving block is fixedly connected with one side of a first moving block through a push rod, a liquid outlet of the liquid conveying cylinder is connected with one end of a liquid outlet pipe, the other end of the liquid outlet pipe is connected with a spray head, a water outlet of the spray head faces downwards, a disc is arranged under the spray head, a plurality of placing grooves are formed in the position, corresponding to the spray head, of the top of the disc at equal intervals along the circumferential direction, gene sequencing chips are placed in the placing grooves, a cylinder body is fixedly installed at the top of the bottom plate, and the bottom end of the cylinder body is rotatably installed at the top of the bottom plate through a bearing seat, the novel belt wheel speed reducer is characterized in that a rod body is arranged in the barrel in a sliding mode, the top end of the rod body extends out of the barrel and is fixedly connected to the bottom of the disc, the rod body and the disc are arranged concentrically, a threaded through hole used for being connected with a bolt in a threaded mode is formed in the outer side of the barrel, a belt wheel is sleeved on the outer side of the barrel, a third motor is fixedly installed at the top of the bottom plate, an output shaft of the third motor is fixedly connected with an input shaft of the speed reducer through a coupler, a belt wheel is installed on an output shaft of the speed reducer, and the belt wheel installed on an output shaft of the speed reducer is connected with the belt wheel which is sleeved on the outer side of the barrel through a belt.

As a further scheme of the invention: the second stirring device comprises a stirring rod, a second motor and a stirring rotating shaft, wherein the stirring rotating shaft is transversely arranged in the box body, one end of the stirring rotating shaft is fixedly connected with an output shaft of the second motor through a coupler, the second motor is fixedly arranged outside the box body through a bolt, and the stirring rod is arranged on the stirring rotating shaft.

As a still further scheme of the invention: the structure and each part specification of the second stirring device and the first stirring device are the same.

As a still further scheme of the invention: the heating pipe is arranged at a position, between the first stirring device and the second stirring device, inside the box body, the two ends of the heating pipe extend out of the box body, the part, located in the box body, of the heating pipe is designed in a spiral mode, and the heating pipe is made of glass materials.

As a still further scheme of the invention: the outer side of the first moving block and the inner wall of the infusion barrel are both in polishing design and are tightly attached to each other.

As a still further scheme of the invention: the disc is provided with a clamping groove which is an annular groove, the disc is provided with a protective cover, and the protective cover is matched with the clamping groove.

As a still further scheme of the invention: the first motor, the second motor and the third motor are respectively and electrically connected with the controller through wires, and the controller is electrically connected with an external power supply through wires.

Compared with the prior art, the invention has the beneficial effects that:

1. the stirring rod on the stirring rotating shaft is driven to rotate by the rotation of the second motor, so that the reagent of the test sample in the box body is stirred and uniformly mixed, the accuracy of a subsequent gene sequencing result is ensured, and the effect of stirring and uniformly mixing the reagent of the test sample in the box body is further improved by arranging the first stirring device;

2. when the test sample reagent in the box body needs to be heated or kept warm, hot water and the like pass through the heating pipe, so that the heating and the heat preservation of the test sample reagent in the box body are realized, the heating pipe is spirally arranged, the contact area between the heating pipe and the test sample reagent is increased, and the efficiency and the effect of heating and heat preservation of the test sample reagent are improved;

3. thereby set up gene sequencing chip and be used for detecting quantity and the sequence information that obtains the sample molecule to test sample reagent, easy operation utilizes the third motor to rotate and then drives the barrel and rotate, further drives the disc and rotates to make the standing groove on the disc on the different positions rotate to just to the shower nozzle, thereby realize the continuation and many times gene sequencing to test sample reagent, detection efficiency is high, has guaranteed gene sequencing's accuracy simultaneously.

Drawings

FIG. 1 is a schematic diagram of an analyzer for lung cancer gene sequencing research based on electronic information technology.

FIG. 2 is a front view of an analyzer for lung cancer gene sequencing research based on electronic information technology.

FIG. 3 is a top view of a disk in an analyzer for lung cancer gene sequencing based on electronic information technology.

FIG. 4 is a schematic structural diagram of a mixing device in an analyzer for lung cancer gene sequencing research based on electronic information technology.

FIG. 5 is a schematic diagram of the structure at A in an analyzer for lung cancer gene sequencing research based on electronic information technology.

FIG. 6 is a schematic diagram of the structure at B in an analyzer for lung cancer gene sequencing research based on electronic information technology.

Shown in the figure: the device comprises a blending device 1, a top plate 2, a first motor 3, a supporting block 4, a bottom plate 5, a cylinder body 6, a rod body 7, a disc 8, a protective cover 9, a spray head 10, a liquid outlet pipe 11, a transfusion barrel 12, a first moving block 13, a valve 14, a lead screw 15, a placing groove 16, a gene sequencing chip 17, a clamping groove 18, a first stirring device 19, a heating pipe 20, a stirring rod 21, a second motor 22, a second stirring device 23, a stirring rotating shaft 24, a box body 25, a third motor 26, a speed reducer 27, a second moving block 28 and a push rod 29.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 6, in an embodiment of the present invention, an analyzer for lung cancer gene sequencing research based on an electronic information technology includes a blending device 1, a top plate 2, a first motor 3, a support block 4, a bottom plate 5, a cylinder 6, a rod body 7, a disc 8, a protective cover 9, a spray head 10, a liquid outlet pipe 11, a liquid delivery cylinder 12, a first moving block 13, a valve 14, a lead screw 15, a placement groove 16, a gene sequencing chip 17, a clamping groove 18, a first stirring device 19, a heating pipe 20, a stirring rod 21, a second motor 22, a second stirring device 23, a stirring rotating shaft 24, a box 25, a third motor 26, a speed reducer 27, a second moving block 28, and a push rod 29, wherein the top plate 2 is fixedly installed at the top of the bottom plate 5 through a support rod, the blending device 1 is arranged at the top of the top plate 2, and the blending device 1 includes a heating pipe 20, a stirring rod 21, a second motor 22, a liquid outlet pipe 11, a liquid outlet pipe 12, a second moving block 28, and a push rod 29, The stirring device comprises a second stirring device 23, a stirring rotating shaft 24 and a box body 25, wherein the box body 25 is fixedly arranged at the top of a top plate 2, a liquid inlet of the box body 25 is connected with one end of a liquid inlet pipe in a pipe manner, a first stirring device 19 and a second stirring device 23 are symmetrically arranged on two sides in the box body 25, the second stirring device 23 comprises a stirring rod 21, a second motor 22 and a stirring rotating shaft 24, the stirring rotating shaft 24 is transversely arranged in the box body 25, one end of the stirring rotating shaft 24 is fixedly connected with an output shaft of the second motor 22 through a coupler, the second motor 22 is fixedly arranged on the outer side of the box body 25 through bolts, the stirring rod 21 is arranged on the stirring rotating shaft 24, the structures and specifications of each part of the second stirring device 23 and the first stirring device 19 are the same, and a heating pipe 20 is arranged at a position between the first stirring device 19 and the second stirring device 23 in the box body 25, the two ends of the heating pipe 20 extend out of the box body 25, the part of the heating pipe 20 located in the box body 25 is designed to be coiled, the heating pipe 20 is made of glass, a controller is fixedly installed on the front surface of the box body 25, a wireless communication module is arranged in the controller, the controller is connected with a mobile terminal of an operator such as a mobile phone through the wireless communication module to realize remote control of equipment, a liquid outlet of the box body 25 is connected with a liquid inlet of the infusion cylinder 12 through a liquid guide pipe, a valve 14 is installed on the liquid guide pipe, the infusion cylinder 12 is fixedly installed on the top of the bottom plate 5 through a support rod, a first moving block 13 is arranged in the infusion cylinder 12 in a sliding mode, the outer side of the first moving block 13 and the inner wall of the infusion cylinder 12 are both designed to be polished and are tightly attached to each other, and a second moving block 28 is arranged in the infusion cylinder 12 at a position located on one side of the first moving block 13 in a sliding mode, a lead screw 15 is transversely arranged in the liquid conveying cylinder 23, one end of the lead screw 15 extends out of the liquid conveying cylinder 12 and is fixedly connected with an output shaft of a first motor 3 through a coupler, the first motor 3 is fixedly installed at the top of a supporting block 4, the supporting block 4 is fixedly installed at the top of a bottom plate 5, the lead screw 15 transversely penetrates through a second moving block 28, a threaded through hole for being in threaded connection with the lead screw 15 is formed in the second moving block 28, one side of the second moving block 28 is fixedly connected with one side of a first moving block 13 through a push rod 29, a liquid outlet of the liquid conveying cylinder 12 is connected with one end of a liquid outlet pipe 11, the other end of the liquid outlet pipe 11 is connected with a spray head 10, a water outlet of the spray head 10 faces downwards, a circular disc 8 is arranged under the spray head 10, a clamping groove 18 is formed in the circular disc 8, the clamping groove 18 is an annular groove, and a protective cover 9 is arranged on the circular disc 8, the protection cover 9 is matched with the clamping groove 18, a plurality of placing grooves 16 are formed in the position, corresponding to the spray head 10, of the top of the disc 8 at equal intervals along the circumferential direction, gene sequencing chips 17 are placed in the placing grooves 16, a barrel 6 is fixedly installed at the top of the bottom plate 5, the bottom end of the barrel 6 is rotatably installed at the top of the bottom plate 5 through a bearing seat, a rod body 7 is arranged in the barrel 6 in a sliding mode, the top end of the rod body 7 extends out of the barrel 6 and is fixedly connected to the bottom of the disc 8, the rod body 7 and the disc 8 are arranged concentrically, a threaded through hole used for being in threaded connection with a bolt is formed in the outer side of the barrel 6, a belt pulley is sleeved on the outer side of the barrel 6, a third motor 26 is fixedly installed at the top of the bottom plate 5, an output shaft of the third motor 26 is fixedly connected with an input shaft of a speed reducer 27 through a coupler, and a belt pulley is installed on an output shaft of the speed reducer 27, the belt pulley of installation on the speed reducer 27 output shaft passes through the belt and is connected with the belt pulley of barrel 6 outside cover establishing, first motor 3, second motor 22 and third motor 26 are connected with the controller electricity through the wire respectively, and the controller is connected with external power supply electricity through the wire.

The working principle of the invention is as follows:

when lung cancer gene sequencing is required, firstly, the protective cover 9 is taken down, a test sample reagent is further sent into the box body 25 through the liquid inlet pipe, the second motor 22 is utilized to rotate to drive the stirring rod 21 on the stirring rotating shaft 24 to rotate, so that the stirring and mixing of the test sample reagent in the box body 25 are realized, the accuracy of a subsequent gene sequencing result is ensured, the first stirring device 19 is arranged to further improve the effect of stirring and mixing the test sample reagent in the box body 25, when the test sample reagent in the box body 25 needs to be heated or heat-preserved, hot water and the like pass through the heating pipe 20, so that the heating and heat-preservation of the test sample reagent in the box body 25 are realized, the contact area between the heating pipe 20 and the test sample reagent is improved by adopting spiral arrangement of the heating pipe 20, the heating and heat-preservation efficiency and effect of the test sample reagent are improved, the valve 14 is opened, make the test sample reagent in the box 25 get into in the infusion section of thick bamboo 12 through the catheter, utilize first motor 3 to rotate and then drive lead screw 15 and rotate, thereby make second movable block 28 remove and further promote first movable block 13 and remove, thereby make the test sample reagent in the infusion section of thick bamboo 12 discharge from shower nozzle 10 after through drain pipe 11, and further fall into standing groove 16, set up gene sequencing chip 17 and be used for detecting test sample reagent thereby obtain the quantity and the sequence information of sample molecule, moreover, the operation is simple, utilize third motor 26 to rotate and then drive barrel 6 and rotate, further drive disc 8 and rotate, thereby make standing groove 16 on the different positions on disc 8 rotate to just right shower nozzle 10, thereby realize the continuation and many times gene sequencing to test sample reagent, high detection efficiency, and the accuracy of gene sequencing has been guaranteed simultaneously.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.