阅读说明：本技术 一种二化螟防控用药渗透剂的筛选方法及应用 (Screening method and application of striped rice borer control drug penetrant ) 是由 吴敏 韩召军 沈子涵 黄水金 于 2018-07-25 设计创作，主要内容包括：本发明公开一种二化螟防控用药渗透剂的筛选方法,将相同浓度的不同渗透剂液体中加入着色剂,测定每种渗透剂渗透通过特定长度的稻茎的时间,其中渗透时间短的渗透剂液体渗透效果优于渗透时间长的渗透剂液体。本发明所述方法是通过测定渗透剂对水稻茎鞘的渗透能力来筛选对防治二化螟药剂具有增效作用的渗透剂,获得对水稻叶鞘和心叶具有较好渗透能力的助剂,为提高化学药剂对二化螟的防治效果,降低化学药剂的使用剂量提供了可能。(The invention discloses a screening method of a striped rice borer prevention and control drug penetrant, which is characterized in that a colorant is added into different penetrant liquids with the same concentration, and the time of each penetrant penetrating through a rice stem with a specific length is measured, wherein the penetrant liquid with short penetrating time has a penetrating effect superior to that of the penetrant liquid with long penetrating time. The method screens the penetrant with synergistic effect on the chemical for preventing and controlling chilo suppressalis by measuring the penetrant capability of the penetrant to the rice stem sheath, obtains the auxiliary agent with better penetrant capability to the rice leaf sheath and the heart leaf, and provides possibility for improving the preventing and controlling effect of the chemical on the chilo suppressalis and reducing the dosage of the chemical.)

1. A method for screening the penetrant for preventing and controlling striped rice borer features that the colouring agent is added to the liquid of different penetrants with same concentration, and the time for each penetrant to penetrate through the rice stem with a certain length is measured, where the penetrant with short penetrating time has higher penetrating effect than the penetrant with long penetrating time.

2. A method for screening a striped rice borer control drug penetrant is characterized by comprising the following steps:

(1) taking rice stems with specific length, trimming rice leaves, only keeping core and leaf parts of the rice stems, wrapping a circle of water absorbing material in the middle of an outer ring of the rice stems, and placing the base parts of the rice stems under the liquid level of water;

(2) adding coloring agent into different penetrant liquids with the same concentration, placing the capillary end of a capillary gun head connected with a capillary hose at the bottom of the penetrant liquid, placing the nozzle of the capillary gun at or above the central leaf of the rice stem, adjusting the height of the penetrant liquid to be higher than that of the capillary gun head, and enabling the penetrant liquid to automatically drop to the central leaf of the rice stem from the nozzle of the capillary gun and infiltrate downwards along the inside of the rice stem;

(3) the time required for the coloring penetrant to seep out from the rice stem base incision from the beginning of dripping to the rice heart leaf was observed and recorded, wherein the penetrant liquid having a short permeation time was more effective in permeation than the penetrant liquid having a long permeation time.

3. A method according to claim 1 or 2, wherein the colorant is an ink.

4. The method of claim 3 wherein said colorant is a red ink.

5. A method according to claim 1 or 2, wherein the volume fraction of the colourant is in the range 0.5% to 2.0%.

6. The method of claim 5, wherein the volume fraction is 1.0% when the colorant is a red ink.

7. A method according to claim 1 or 2, wherein the concentration of the osmotic agent liquid is between 0.6% and 1.0%, preferably 0.8%.

8. A method according to claim 1 or 2, wherein the specific length is 8-15 cm, preferably 10 cm.

9. The method of claim 2, wherein the water absorbent material is paper towel or cotton.

10. The chemical for preventing and controlling chilo suppressalis is characterized by comprising an effective amount of an active chemical and a penetrating agent, wherein the penetrating agent is selected from a rapid penetrating agent T70, a wetting agent GY-WS10, a super-strong penetrating agent JFC-E or a penetrating agent GY-W07.

Technical Field

The invention relates to the field of pesticides, and particularly relates to a screening method of a striped rice borer control pesticide penetrant and an application penetrant.

Background

Chilo suppressalis Chlio suppersalis (walker) is an important pest on rice and is harmful to larvae boring stalks to take food. At present, chemical control is an important measure for controlling chilo suppressalis, but pests, the pesticide liquid of which cannot permeate into rice stalks, are harmful, so that pesticide application is wasted and the control effect is low. The prior art generally improves the efficacy of the drug by increasing the attachment of the drug to the plant surface, but this method has no effect on boring chilo suppressalis larvae. Therefore, if a certain auxiliary agent can be added into the control agent, the auxiliary agent can improve the dosage of the chemical agent entering the habitat parts (in the leaf sheath and the heart leaf) of the chilo suppressalis larvae, so that the exposure chance of the chemical agent to the chilo suppressalis larvae can be increased, the dosage of the chemical agent to the chilo suppressalis larvae is improved, and the chemical agent can exert better control effect. However, the variety of the auxiliary agents is various, the cost is too high through a complex or direct prevention and control experiment, how to adopt a simple screening method to screen the auxiliary agents, and the screening result has reliable correlation with a real result is a key problem to be solved.

Disclosure of Invention

The invention aims to establish a screening method of a penetrant with a synergistic effect on a chemical for preventing and treating chilo suppressalis. The method screens the penetrant with synergistic effect on the chemical for preventing and controlling chilo suppressalis by measuring the penetrant capability of the penetrant to the rice stem sheath, obtains the auxiliary agent with better penetrant capability to the rice leaf sheath and the heart leaf, and provides possibility for improving the preventing and controlling effect of the chemical on the chilo suppressalis and reducing the dosage of the chemical.

The purpose of the invention can be realized by the following technical scheme: a method for screening the penetrant for preventing and controlling striped rice borer includes such steps as adding colouring agent to the liquid of different penetrants with same concentration, and measuring the time for each penetrant to penetrate through the rice stem with a certain length.

The invention also provides a more specific screening method of the striped rice borer control drug penetrant, which comprises the following steps:

(1) taking rice stems with specific length, trimming rice leaves, only keeping core and leaf parts of the rice stems, wrapping a circle of water absorbing material in the middle of an outer ring of the rice stems, and placing the base parts of the rice stems under the liquid level of water;

(2) adding coloring agent into different penetrant liquids with the same concentration, placing the capillary end of a capillary gun head connected with a capillary hose at the bottom of the penetrant liquid, placing the nozzle of the capillary gun at or above the central leaf of the rice stem, adjusting the height of the penetrant liquid to be higher than that of the capillary gun head, and enabling the penetrant liquid to automatically drop to the central leaf of the rice stem from the nozzle of the capillary gun and infiltrate downwards along the inside of the rice stem;

(3) the time required for the coloring penetrant to seep out from the rice stem base incision from the beginning of dripping to the rice heart leaf was observed and recorded, wherein the penetrant liquid having a short permeation time was more effective in permeation than the penetrant liquid having a long permeation time.

The colorant of the present invention is a colorant that is visible to the naked eye and does not affect a penetrant, and from the viewpoint of cost reduction and a combined effect, the present invention is preferably an ink, and more preferably a red ink for easy observation. The final concentration of the colorant can be adjusted for different colorants, preferably with the lowest volume fraction that can be clearly seen, typically in the range of 0.5% to 2.0%, more preferably 1.0% in the case of red inks.

The concentration of the penetrant liquid is preferably 0.6-1.0%, more preferably 0.8%, and the correlation between the measured result and the actual result is better.

The specific length of the invention is preferably 8-15 cm, preferably 10cm, and the performance of each penetrating agent can be effectively distinguished in the range.

The water absorbing material of the present invention may be a common water absorbing material which is convenient to fix and is used for preventing the liquid medicine from flowing down from the outside, such as paper towels, cotton, etc., and the paper towels are preferred.

The invention also provides a chilo suppressalis control medicament screened by the method, which comprises an effective amount of an active medicament and a penetrant, wherein the penetrant is selected from a rapid penetrant T70, a wetting agent GY-WS10, a super penetrant JFC-E or a penetrant GY-W07.

Penetrant the penetrant of the present invention is selected to have a good penetration rate to rice stems according to the penetration time. The invention verifies the synergistic capability of the permeation aid to chemical agents and the correlation between the synergistic effect of the permeation aid to the agents and the infiltration rate of the permeation aid to the agents in rice stem sheaths by utilizing an indoor bioassay experiment, and finally verifies the synergistic effect of the effective permeation aid screened by utilizing the method to the chemical agents again by utilizing a field plot experiment, thereby verifying that the permeation aid screened by utilizing the rice stem permeation rate method to screen the permeation aid for the synergism of the pesticide for preventing and controlling rice stem borers is feasible and effective. The method is simple and easy to implement, is convenient to operate, and has important significance for improving the control effect of the pesticide in the chilo suppressalis.

Penetrant

Drawings

FIG. 1 is a schematic view of a device for measuring the permeability of a penetrant to rice stalks.

Detailed Description

The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.