阅读说明：本技术 一种三叶木通果皮中矿物元素含量的测定方法 (Method for determining content of mineral elements in akebia trifoliata peels ) 是由 张孟琴 龙金花 石庆丹 罗彪 于 2019-07-15 设计创作，主要内容包括：本发明公开了一种三叶木通果皮中矿物元素含量的测定方法,称取120℃干燥至恒重的芦丁对照品5.00MG,用50％的乙醇溶解,用50％的乙醇定容,吸取上述溶液,加入1.00ml 50％的乙醇,在室温高于10℃的条件下放置10min后,最后用50％的乙醇溶液定容,摇匀,放置10～15min,以试剂空白做参比,在510nm处测吸光度；称取三叶木通果皮粉末2.00g,加入40ml50％乙醇溶液,在80℃水浴中回流2h,离心,滤液定容于50ml容量瓶中,作为试样液,按标准溶液的铝盐显色法在510nm下测其吸光度,本发明简便实用、费用低廉、效率高。(The invention discloses a method for measuring the content of mineral elements in akebia trifoliata peels, weighing a rutin reference substance 5.00MG dried to constant weight at 120 ℃, dissolving the rutin reference substance with 50% ethanol, fixing the volume with 50% ethanol, sucking the solution, adding 1.00ml of 50% ethanol, placing the solution for 10min at the room temperature higher than 10 ℃, fixing the volume with 50% ethanol solution, shaking up, placing for 10-15 min, taking the blank of a reagent as a reference, and measuring the absorbance at 510 nm; weighing 2.00g of akebia trifoliata peel powder, adding 40ml of 50% ethanol solution, refluxing for 2h in 80 ℃ water bath, centrifuging, containing filtrate in a 50ml volumetric flask as sample solution, and measuring the absorbance of the sample solution at 510nm according to an aluminum salt color development method of a standard solution.)

1. A method for measuring the content of mineral elements in akebia trifoliate peels is characterized by comprising the following steps: the method comprises the following steps:

1: weighing rutin control 5.00MG dried at 120 deg.C to constant weight, dissolving with 50% ethanol, transferring into 50ML volumetric flask, adding 50% ethanol to constant volume, and shaking (rho is 0.1 g. L-1);

2: accurately sucking 0, 0.50, 1.00, 1.50, 2.00, 2.50, and 3.00ml of the above solutions, placing in 10ml volumetric flasks, adding 1.00ml of 50% ethanol, and adding 0.3ml of 5% NaNo₂Shaking the solution, standing at room temperature higher than 10 deg.C for 10min, adding 10% Al (NO)₂)3, shaking the solution for 10min, adding 4ml of 4% NaoH solution respectively, fixing the volume by using 50% ethanol solution, shaking the solution for 10-15 min, taking a reagent blank as a reference, and measuring the absorbance at 510 nm;

3: weighing 2.00g of akebia trifoliata peel powder, adding 40ml of 50% ethanol solution, refluxing for 2h in a water bath at 80 ℃, centrifuging, fixing the filtrate in a 50ml volumetric flask to serve as a sample solution, measuring the absorbance of the sample solution at 510nm according to an aluminum salt color development method of a standard solution, calculating the concentration of flavone in akebia trifoliata peel extract by a regression equation of a standard curve, and further calculating the content of the flavone;

4: the akebia trifoliata peel flavone is extracted by adopting an ethanol water solution, single-factor experimental study is carried out on the feed-liquid ratio, the extraction temperature, the extraction time, the ethanol concentration and the like which influence the extraction rate factor, and then orthogonal experiment is horizontally carried out to select the optimal extraction condition.

2. The method for determining the content of mineral elements in akebia trifoliata peels as claimed in claim 1, wherein the method comprises the following steps: and performing a linear regression equation on the absorbance value to obtain a standard curve for drawing the absorbance concentration.

3. The method for determining the content of mineral elements in akebia trifoliata peels as claimed in claim 2, wherein the method comprises the following steps: the regression equation of the standard curve is that A is 0.0081C +0.0097, R2 is 0.9964, and R is 0.9982.

Technical Field

The invention relates to a method for measuring the content of mineral elements in pericarp, in particular to a method for measuring the content of mineral elements in the pericarp of akebia trifoliata.

Background

Wild akebia trifoliate belongs to akebia deciduous woody vine. The fruit pulp is like a fragrant burnt fruit, so the fruit pulp is named as a wild banana, the pulp is juicy and sweet, and the fresh fruit contains rich protein, starch, soluble sugar, organic acid, amino acid and the like necessary for human bodies, so the fruit pulp has higher nutritional value. As a fruit resource, akebia trifoliata fruits are thick in peel and many in seeds, and the peel accounts for 69.1% of the total weight of the fruits, and is discarded in most cases.

Research reports that the flavonoid compounds have wide application in the aspects of medicines and the like, and have the effects of resisting aging, inhibiting blood coagulation, resisting cancer, resisting bacteria and diminishing inflammation, promoting secretion of estrogen and the like. If the flavone with wide application can be extracted from the akebia trifoliata peels, the problem of environmental pollution caused by discarding the akebia trifoliata peels is effectively solved, the economic benefit of the akebia trifoliata is obviously improved, and the akebia trifoliata peels can be used as an effective way for comprehensively utilizing the akebia trifoliata.

Disclosure of Invention

Technical problem to be solved

In order to overcome the defects of the prior art, the method for measuring the content of the mineral elements in the peel of the akebia trifoliata is provided, and is simple, convenient, practical, low in cost and high in efficiency.

(II) technical scheme

The invention is realized by the following technical scheme: the invention provides a method for measuring the content of mineral elements in akebia trifoliata peels, which comprises the following steps:

1: weighing rutin control 5.00MG dried at 120 deg.C to constant weight, dissolving with 50% ethanol, transferring into 50ML volumetric flask, adding 50% ethanol to constant volume, and shaking (rho is 0.1 g. L-1);

2: accurately sucking 0, 0.50, 1.00, 1.50, 2.00, 2.50, and 3.00ml of the above solutions, placing in 10ml volumetric flasks, adding 1.00ml of 50% ethanol, and adding 0.3ml of 5% NaNo₂Shaking the solution, standing at room temperature higher than 10 deg.C for 10min, adding 10% Al (NO)₂)3, shaking the solution for 10min, adding 4ml of 4% NaoH solution respectively, fixing the volume by using 50% ethanol solution, shaking the solution for 10-15 min, taking a reagent blank as a reference, and measuring the absorbance at 510 nm;

3: weighing 2.00g of akebia trifoliata peel powder, adding 40ml of 50% ethanol solution, refluxing for 2h in a water bath at 80 ℃, centrifuging, fixing the filtrate in a 50ml volumetric flask to serve as a sample solution, measuring the absorbance of the sample solution at 510nm according to an aluminum salt color development method of a standard solution, calculating the concentration of flavone in akebia trifoliata peel extract by a regression equation of a standard curve, and further calculating the content of the flavone;

4: the akebia trifoliata peel flavone is extracted by adopting an ethanol water solution, single-factor experimental study is carried out on the feed-liquid ratio, the extraction temperature, the extraction time, the ethanol concentration and the like which influence the extraction rate factor, and then orthogonal experiment is horizontally carried out to select the optimal extraction condition.

Further, the absorbance value is used as a linear regression equation to obtain a standard curve for drawing the absorbance concentration.

Further, the regression equation of the standard curve is that a is 0.0081C +0.0097, R2 is 0.9964, and R is 0.9982.

(III) advantageous effects

Compared with the prior art, the invention has the following beneficial effects:

1. simple and practical, low cost and high efficiency.

2. Not only effectively solves the problem of environmental pollution caused by discarding the pericarp of the akebia trifoliata, but also can obviously improve the economic benefit of the akebia trifoliata.

Detailed Description

The present invention will be described in further detail in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The invention provides a method for measuring the content of mineral elements in akebia trifoliata peels, which comprises the following steps:

1: weighing rutin control 5.00MG dried at 120 deg.C to constant weight, dissolving with 50% ethanol, transferring into 50ML volumetric flask, adding 50% ethanol to constant volume, and shaking (rho is 0.1 g. L-1);

2: accurately sucking 0, 0.50, 1.00, 1.50, 2.00, 2.50, and 3.00ml of the above solutions, placing in 10ml volumetric flasks, adding 1.00ml of 50% ethanol, and adding 0.3ml of 5% NaNo₂Shaking the solution, standing at room temperature higher than 10 deg.C for 10min, adding 10% Al (NO)₂) 0.3ml of 3 solution, shaking up, standing for 10min, then respectively adding 4ml of 4% NaoH solution, finally fixing the volume with 50% ethanol solution, shaking up, standing for 10-15 min, taking a reagent blank as a reference, and measuring the volume at 510nmMeasuring the absorbance;

3: weighing 2.00g of akebia trifoliata peel powder, adding 40ml of 50% ethanol solution, refluxing for 2h in a water bath at 80 ℃, centrifuging, fixing the filtrate in a 50ml volumetric flask to serve as a sample solution, measuring the absorbance of the sample solution at 510nm according to an aluminum salt color development method of a standard solution, calculating the concentration of flavone in akebia trifoliata peel extract by a regression equation of a standard curve, and further calculating the content of the flavone;

4: the akebia trifoliata peel flavone is extracted by adopting an ethanol water solution, single-factor experimental study is carried out on the feed-liquid ratio, the extraction temperature, the extraction time, the ethanol concentration and the like which influence the extraction rate factor, and then orthogonal experiment is horizontally carried out to select the optimal extraction condition.

Preferably, the absorbance value is subjected to a linear regression equation to obtain a standard curve for drawing the absorbance concentration.

Preferably, the regression equation of the standard curve is that a is 0.0081C +0.0097, R2 is 0.9964, and R is 0.9982.

The process flow comprises the following steps: weighing rutin control 5.00MG dried at 120 deg.C to constant weight, dissolving with 50% ethanol, transferring into 50ML volumetric flask, adding 50% ethanol to constant volume, and shaking (rho is 0.1 g. L-1); accurately sucking 0, 0.50, 1.00, 1.50, 2.00, 2.50, and 3.00ml of the above solutions, placing in 10ml volumetric flasks, adding 1.00ml of 50% ethanol, and adding 0.3ml of 5% NaNo₂Shaking the solution, standing at room temperature higher than 10 deg.C for 10min, adding 10% Al (NO)₂)3, shaking the solution for 10min, adding 4ml of 4% NaoH solution respectively, fixing the volume by using 50% ethanol solution, shaking the solution for 10-15 min, taking a reagent blank as a reference, and measuring the absorbance at 510 nm; weighing 2.00g of akebia trifoliata peel powder, adding 40ml of 50% ethanol solution, refluxing for 2h in a water bath at 80 ℃, centrifuging, fixing the filtrate in a 50ml volumetric flask to serve as a sample solution, measuring the absorbance of the sample solution at 510nm according to an aluminum salt color development method of a standard solution, calculating the concentration of flavone in akebia trifoliata peel extract by a regression equation of a standard curve, and further calculating the content of the flavone; the method adopts ethanol water solution to extract akebia trifoliata peel flavone, and performs single-factor experimental research on material-liquid ratio, extraction temperature, extraction time, ethanol concentration and the like which influence the extraction rate factorOrthogonal experiments were then performed horizontally to select the best extraction conditions.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention and do not limit the spirit and scope of the present invention. Various modifications and improvements of the technical solutions of the present invention may be made by those skilled in the art without departing from the design concept of the present invention, and the technical contents of the present invention are all described in the claims.