阅读说明：本技术 一种血细胞分析仪用血红蛋白浓度测定专用试剂 (Special reagent for measuring hemoglobin concentration for blood cell analyzer ) 是由 方啟龙 胡利民 于 2019-09-09 设计创作，主要内容包括：本发明属于血液分析技术领域,涉及一种血细胞分析仪用血红蛋白浓度测定专用试剂,包括阳离子表面活性剂、非离子表面活性剂、缓冲剂和稳定剂,其中阳离子表面活性剂含量为1～40g/L,非离子表面活性剂含量为0.5～20g/L,缓冲剂含量为0.5～20g/L,稳定剂含量为0.1～10g/L。溶解红细胞,释放血红蛋白,并与血红蛋白特异性结合,形成稳定的血红蛋白复合物,在一定的波长下进行比色,其中溶液吸光度的变化与血红蛋白含量成正比,进而对血红蛋白进行定量测定,由此准确测定血红蛋白含量,无有毒成分,不会对皮肤、人体产生危害；其保存时间久、材料易得,性能稳定,有很好的抗菌性能,成本较低,适于工业化生产。(The invention belongs to the technical field of blood analysis, and relates to a special reagent for measuring hemoglobin concentration for a hemocyte analyzer, which comprises a cationic surfactant, a nonionic surfactant, a buffering agent and a stabilizing agent, wherein the content of the cationic surfactant is 1-40 g/L, the content of the nonionic surfactant is 0.5-20 g/L, the content of the buffering agent is 0.5-20 g/L, and the content of the stabilizing agent is 0.1-10 g/L. The method comprises the following steps of (1) dissolving red blood cells, releasing hemoglobin, specifically combining with the hemoglobin to form a stable hemoglobin compound, carrying out color comparison under a certain wavelength, wherein the change of absorbance of a solution is in direct proportion to the content of the hemoglobin, and further carrying out quantitative determination on the hemoglobin, so that the content of the hemoglobin is accurately determined, toxic components are not contained, and harm to skin and human bodies is avoided; the antibacterial agent has the advantages of long storage time, easily available materials, stable performance, good antibacterial performance and low cost, and is suitable for industrial production.)

1. A special reagent for measuring hemoglobin concentration for a blood cell analyzer is characterized in that: comprises a cationic surfactant, a nonionic surfactant, a buffering agent and a stabilizing agent;

wherein the content of the cationic surfactant is 1-40 g/L;

the content of the nonionic surfactant is 0.5-20 g/L;

the content of the buffer is 0.5-20 g/L;

the content of the stabilizer is 0.1-10 g/L.

2. The reagent dedicated to measurement of hemoglobin concentration for a blood cell analyzer according to claim 1, wherein: the cationic surfactant has the structure:

wherein R1, R2 and R4 are-CH 3, R3 is a hydrocarbon group with 4-18 carbon atoms, and the cationic surfactant is preferably one or more of the above.

3. The reagent dedicated to measurement of hemoglobin concentration for a blood cell analyzer according to claim 1, wherein: the nonionic surfactant is preferably one or more of polyoxyethylene type nonionic surfactants.

4. The reagent dedicated to measurement of hemoglobin concentration for a blood cell analyzer according to claim 1, wherein: the buffer is preferably selected from one or more of inorganic salt buffer, organic acid buffer, organic alkali buffer and sulfonate buffer;

the inorganic salt buffer at least comprises one of a boric acid-borax buffer and a phosphate buffer, the organic acid buffer at least comprises one of formic acid, acetic acid, tartaric acid, malonic acid, succinic acid and oxalic acid, and the organic alkali buffer at least comprises one of Tris-HCl, ethanolamine, triethanolamine, imidazoles and Good's buffers.

5. The reagent dedicated to measurement of hemoglobin concentration for a blood cell analyzer according to claim 1, wherein: the stabilizer is a ketone compound, and the ketone compound at least contains a nitrogen-containing heterocyclic ring or a benzene ring structure.

6. The reagent dedicated to measurement of hemoglobin concentration for a blood cell analyzer according to claim 1, wherein: the stabilizer is used to stabilize the hemoglobin concentration.

Technical Field

The invention relates to the technical field of blood analysis, in particular to a special reagent for measuring hemoglobin concentration for a blood cell analyzer.

Background

Hemoglobin concentration measurement is a clinical routine examination item, and is generally measured by means of blood examination, and hemoglobin content can reflect the degree of anemia and is an important basis for diagnosing related diseases.

The hemoglobin concentration determination reagent is matched with a blood cell analyzer for use, is the basis for ensuring the blood analyzer to detect accurate blood parameters, is also a main reagent in a matching reagent of the blood cell analyzer, can be used for determining the concentration of hemoglobin in blood, and can also be used for determining the quantity of white blood cells in the blood.

A hemocyte analyzer measures the concentration of hemoglobin by adopting a colorimetric principle, a cyanide methemoglobin method is usually adopted for detecting the hemoglobin in the early stage, the fatal defects of the method are that a cyanide reagent is extremely toxic, pollutes the environment and is easy to cause public nuisance, and the method is basically stopped being used at present. The existing hemoglobin detection reagent matched with a hemocyte analyzer generally takes a cationic surfactant or an anionic surfactant as a main component, destroys red blood cells to release hemoglobin, and forms a compound with the hemoglobin to perform colorimetric detection, wherein the cationic surfactant detection reagent is used, the compound formed by the cationic surfactant and the hemoglobin has poor absorbance stability under a specific wavelength, the absorbance is reduced along with the increase of time, the measurement result is low, the anionic surfactant detection reagent needs to be added with an antibacterial agent, and the detection result is influenced after a plurality of antibacterial agents are added.

In view of the above problems, the present invention provides a reagent for measuring hemoglobin concentration for blood cell analysis.

Disclosure of Invention

The present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a reagent for measuring hemoglobin concentration for a blood cell analyzer.

In order to achieve the purpose, the invention provides the following technical scheme: a special reagent for measuring hemoglobin concentration for a blood cell analyzer comprises a cationic surfactant, a nonionic surfactant, a buffering agent and a stabilizing agent;

wherein the content of the cationic surfactant is 1-40 g/L;

the content of the nonionic surfactant is 0.5-20 g/L;

the content of the buffer is 0.5-20 g/L;

the content of the stabilizer is 0.1-10 g/L.

Preferably, the cationic surfactant has the structure:

wherein R is₁、R₂、R₄Are all-CH₃，R₃The cationic surfactant is a hydrocarbon group with 4-18 carbon atoms, and the cationic surfactant is preferably one or more of the cationic surfactants.

Preferably, the nonionic surfactant is one or more of polyoxyethylene nonionic surfactants.

Preferably, the buffer is one or more selected from inorganic salt buffer, organic acid buffer, organic alkali buffer and sulfonate buffer;

the inorganic salt buffer at least comprises one of a boric acid-borax buffer and a phosphate buffer, the organic acid buffer at least comprises one of formic acid, acetic acid, tartaric acid, malonic acid, succinic acid and oxalic acid, and the organic alkali buffer at least comprises one of Tris-HCl, ethanolamine, triethanolamine, imidazoles and Good's buffers.

Preferably, the stabilizer is a ketone compound, and the ketone compound at least contains a nitrogen-containing heterocyclic ring or a benzene ring structure.

Preferably, the stabilizer is used for stabilizing the hemoglobin concentration from decreasing.

Compared with the prior art, the invention has the beneficial effects that: a special reagent for measuring hemoglobin concentration for a blood cell analyzer comprises a cationic surfactant, a nonionic surfactant, a buffering agent and a stabilizing agent, is mainly used for dissolving red blood cells, releasing hemoglobin, and specifically combining with the hemoglobin to form a stable hemoglobin compound in the presence of the stabilizing agent, and performs color comparison at a certain wavelength, wherein the change of absorbance of a solution is in direct proportion to the content of the hemoglobin, so that the hemoglobin is quantitatively measured, and the content of the hemoglobin is accurately measured;

the cationic surfactant can be alkyl trimethyl ammonium chloride and alkyl trimethyl ammonium bromide, has the function of dissolving erythrocytes, and can be combined with hemoglobin to form a stable compound in the presence of a stabilizer;

the nonionic surfactant can be fatty acid polyoxyethylene ether, fatty amine polyoxyethylene ether or fatty alcohol polyoxyethylene ether, can increase the solubility of component components, can accelerate the formation of a hemoglobin compound, and simultaneously can prevent the cationic surfactant from generating turbidity or precipitation under the action of a blood sample, so that the probability of plugging holes of an instrument is reduced;

the buffer can be boric acid-borax buffer, phosphate buffer, formic acid buffer, acetic acid buffer, tartaric acid buffer, malonic acid buffer, succinic acid buffer and oxalic acid buffer, and the special reagent for measuring the hemoglobin concentration for the hematology analyzer is kept at a stable pH value;

when the special reagent for measuring the concentration of the hemoglobin for the blood cell analyzer is mixed with blood, the compound formed by the cationic surfactant and the hemoglobin is kept unchanged by the stabilizer within the detection time of the analyzer, so that the detection result is more accurate and stable;

no toxic component is generated in the using process, and no harm is generated to the skin and the human body; the antibacterial agent has the advantages of long storage time, easily available materials, stable performance, good antibacterial performance and low cost, and is suitable for industrial production.

Drawings

FIG. 1 is a graph relating hemoglobin concentration measured by a reagent prepared in example 1 of the present invention and a reagent matched with a BC-5800 blood cell analyzer;

FIG. 2 is a graph relating hemoglobin concentration measured by the prepared reagent and the BC-5180 hematology analyzer matching reagent in example 2 of the present invention;

FIG. 3 is a graph showing the correlation between the hemoglobin concentration measured by the prepared reagent and the BC-1800 hematology analyzer matching reagent in example 3 of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.