阅读说明：本技术 一种从白刺果种子油中提取不饱和脂肪酸的方法 (Method for extracting unsaturated fatty acid from nitraria tangutorum bobr seed oil ) 是由 马烨 袁增辉 李冬冬 赵婌君 于 2019-10-10 设计创作，主要内容包括：本发明公开了一种从白刺果种子油中提取不饱和脂肪酸的方法,将白刺果种子清洗、烘干后,置于粉碎机中粉碎后,过筛；将过筛的白刺果种子和一定量的萃取液,置于萃取釜中静态萃取一段时间后,收料,制得不饱和脂肪酸。本发明以打碎后的白刺果种子为对象,与萃取液混合后在一定的压力和温度下,采用超临界萃取法萃取白刺籽油,以实现从白刺果种子油中提取不饱和脂肪酸。其操作步骤简单易行,过程中没有引入污染；本提取方法选取最合适的萃取压力,温度与时间,既提高了萃取效率,又保证了产品的纯度,又能保证营养物质不受破坏,有效防止高温对活性成分的影响,为白刺果种子油的综合利用和深开发提供技术支持,具有很强的实用性和广泛的适用性。(The invention discloses a method for extracting unsaturated fatty acid from nitraria seed oil, comprising the steps of cleaning nitraria seed, drying, crushing in a crusher, and sieving; placing the sieved nitraria tangutorum bobr seeds and a certain amount of extract liquor into an extraction kettle for static extraction for a period of time, and collecting the extract liquor to prepare unsaturated fatty acid. The method takes the smashed nitraria tangutorum bobr seeds as an object, and adopts a supercritical extraction method to extract the nitraria tangutorum bobr seed oil after mixing with an extraction liquid under certain pressure and temperature so as to realize the extraction of unsaturated fatty acid from the nitraria tangutorum bobr seed oil. The operation steps are simple and easy to implement, and no pollution is introduced in the process; the extraction method selects the most appropriate extraction pressure, temperature and time, not only improves the extraction efficiency, but also ensures the purity of the product, can ensure that nutrient substances are not damaged, effectively prevents the influence of high temperature on active ingredients, provides technical support for the comprehensive utilization and deep development of the nitraria tangutorum bobr seed oil, and has strong practicability and wide applicability.)

1. A method for extracting unsaturated fatty acid from nitraria tangutorum bobr seed oil is characterized by comprising the following steps:

s1, cleaning and drying the nitraria tangutorum bobr seeds;

s2, placing the dried nitraria tangutorum bobr seeds into a grinder to be ground and then sieving the ground nitraria tangutorum bobr seeds;

s3, placing the sieved nitraria tangutorum bobr seeds and a certain amount of extract liquid into an extraction kettle for static extraction for a period of time, and collecting the extract liquid to obtain unsaturated fatty acid.

2. The method as claimed in claim 1, wherein the drying in step S1 is performed at 110-130 deg.C for 2-4 h.

3. The method for extracting unsaturated fatty acids from Nitraria tangutorum bobr seed oil as claimed in claim 1, wherein the sieving in step S2 is 60-80 mesh.

4. The method for extracting unsaturated fatty acids from nitraria tangutorum bobr seed oil as claimed in claim 1, wherein the extract in the step S3 comprises n-hexane: ethyl acetate ═ 9: 1.

5. the method for extracting unsaturated fatty acid from Nitraria sibirica seed oil according to claim 1, wherein the liquid-to-liquid ratio of the extraction liquid in the step S3 to Nitraria sibirica seed is 40-60 (L): 1 (kg).

6. The method for extracting unsaturated fatty acids from Nitraria sibirica seed oil as claimed in claim 1, wherein the extraction in step S3 is performed under 30-50MPa, at 30-65 deg.C and for 2-4 h.

7. The method of claim 1, wherein the step S3 of collecting is to maintain CO₂And collecting the product from a discharge hole under the condition of the flow rate of 2.5L/min.

Technical Field

The invention relates to a method for extracting unsaturated fatty acid, in particular to a method for extracting unsaturated fatty acid from nitraria tangutorum bobr seed oil.

Background

The nitraria tangutorum bobr belongs to the genus nitraria and the family Zygophyllaceae, and is a multi-branch saline-alkali-resistant droughty type short shrub. Nitraria sibirica pall is usually grown in salinization areas in desert regions and has the physiological characteristics of drought resistance, high temperature resistance, strong water retention capacity and the like. The white thorns are excellent sand-fixing shrub plants in the sand areas of China and are important research objects in wind and sand protection. The fruits are oval like soybean grains, are dark red or orange yellow after being ripened, can be directly eaten, are called desert cherries and are called third-class fruits.

The nitraria tangutorum bobr has rich resources and wide distribution, and is wild in the places of Zhangjiakou, Tianjin, Cangzhou and the like in the northwest desert area of China and the coastal areas of North China and northeast China. According to statistics, the nitraria tangutorum bobr basins are distributed in pollution-free areas with the altitude of more than 3000 m, 6-10 ten thousand tons of nitraria tangutorum bobr can be produced every year, and the method has great development value. There are records as early as in compendium of materia Medica: bai Ci has pungent, cold and nontoxic flavor, and can treat swelling and abscess, promote pus discharge, tonify kidney qi and replenish essence and marrow. But the people really began to pay attention to the nutritional value of the nitraria tangutorum bobr after the 90 s of the 20 th century. Researches show that the content of crude protein in the nitraria tangutorum bobr is 10.6-14.4%, the content of fat and fruit oil in pulp is 3.5%, the content of vitamin C also reaches 7.8-18.3%, more importantly, the nitraria tangutorum bobr also contains abundant nutrient substances such as protein, fat, sugar and the like, and the content of flavone is up to 1.1-1.9%.

In the Nitraria tangutorum bobr resources, Nitraria tangutorum bobr seeds are an important part of Nitraria tangutorum bobr fruits, and a large amount of Nitraria tangutorum bobr seeds can be produced when Nitraria tangutorum bobr fruits are utilized, and researches show that Nitraria tangutorum bobr seeds contain various nutritional ingredients and active substances and also contain a large amount of fatty acids, particularly unsaturated fatty acids of which the content is more than 90%. Unsaturated fatty acids are a kind of fatty acids constituting body fat, and are essential fatty acids for the human body. It is important to prevent atherosclerosis because of its ability to lower blood cholesterol. It has been found that cholesterol must be bound to unsaturated fatty acids before proper functioning and metabolism can occur in vivo. If the unsaturated fatty acid is lacked, cholesterol is combined with the saturated fatty acid to generate metabolic disorder, and is deposited on the blood vessel wall to gradually form atherosclerosis, thereby causing cardiovascular and cerebrovascular diseases.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention aims to provide a method for extracting unsaturated fatty acid from nitraria tangutorum bobr seed oil.

In order to achieve the above object, the present invention adopts the following technical solutions:

a method for extracting unsaturated fatty acid from Nitraria tangutorum bobr seed oil comprises the following steps:

s1, cleaning and drying the nitraria tangutorum bobr seeds;

s2, placing the dried nitraria tangutorum bobr seeds into a grinder to be ground and then sieving the ground nitraria tangutorum bobr seeds;

s3, placing the sieved nitraria tangutorum bobr seeds and a certain amount of extract liquid into an extraction kettle for static extraction for a period of time, and collecting the extract liquid to obtain unsaturated fatty acid.

The drying in the step S1 is performed at a temperature of 110-130 ℃ for 2-4h, preferably at a temperature of 120 ℃ for 2 h.

The screen in step S2 is 60 to 80 mesh, preferably 60 mesh.

The extraction liquid in the step S3 includes n-hexane: ethyl acetate ═ 9: 1

The liquid-material ratio of the extraction liquid in the step S3 to the nitraria tangutorum bobr seeds is 40-60 (L): 1(kg), preferably 50 (L): 1 (kg).

The extraction in the step S3 is carried out under the pressure of 30-50MPa, preferably 35MPa, at the temperature of 30-65 ℃ and for 2-4 h.

The material collection in the step S3 is to maintain CO₂And collecting the product from a discharge hole under the condition of the flow rate of 2.5L/min.

The invention has the advantages that:

the invention relates to a method for extracting unsaturated fatty acid from nitraria tangutorum bobr seed oil, which takes smashed nitraria tangutorum bobr seeds as an object, mixes the smashed nitraria tangutorum bobr seeds with an extraction liquid, and extracts the nitraria tangutorum bobr seed oil by adopting a supercritical extraction method under certain pressure and temperature so as to realize the extraction of the unsaturated fatty acid from the nitraria tangutorum bobr seed oil. The operation steps are simple and easy to implement, and no pollution is introduced in the process; the extraction method selects the most appropriate extraction pressure, temperature and time, not only improves the extraction efficiency, but also ensures the purity of the product, can ensure that nutrient substances are not damaged, effectively prevents the influence of high temperature on active ingredients, provides technical support for the comprehensive utilization and deep development of the nitraria tangutorum bobr seed oil, and has strong practicability and wide applicability.

Detailed Description

The following specific examples are intended to illustrate the invention.

A method for extracting unsaturated fatty acid from Nitraria tangutorum bobr seed oil comprises the following steps: impurity removal and drying (S1) → pulverization treatment (S2) → static extraction (S3) → collection → finish product.

S1, cleaning the nitraria tangutorum bobr seeds, removing impurities, cleaning, and baking for 2-4h in a drying box at the temperature of 110-;

s2, placing the clean and dry nitraria tangutorum bobr seeds into a grinder to be ground, and then sieving the ground nitraria tangutorum bobr seeds by a sieve of 60-80 meshes for later use;

s3, sieving the nitraria tangutorum seeds and a certain amount of extract liquid, and mixing the sieved nitraria tangutorum seeds and the extract liquid in a ratio of 40-60 (L): 1(kg) of material-liquid ratio, placing the mixture in an extraction kettle, and performing static extraction for 2-4h under the environment of 30-50MPa and 30-65 ℃; in the maintenance of CO₂And collecting the product from a discharge hole under the condition of the flow rate of 2.5L/min to prepare the unsaturated fatty acid.

The extraction liquid adopts n-hexane (component 1): ethyl acetate (component 2) ═ 9: 1