阅读说明：本技术 明胶/cmc昆虫病毒微胶囊的制备方法 (Preparation method of gelatin/CMC insect virus microcapsule ) 是由 蒲侠 朱丹丹 周新华 舒绪刚 于 2019-09-18 设计创作，主要内容包括：本发明公开一种明胶/CMC昆虫病毒微胶囊的制备方法,包括以下步骤：(1)将CMC溶于去离子水中制得CMC溶液；在CMC溶液加入昆虫病毒干粉,搅拌混合均匀,形成混合物A；用即配的缓冲溶液配制质量分数为1％-5％的明胶溶液；(2)将明胶溶液加入混合物A中,明胶与CMC的质量比为1:2～12:1；在25～35℃温度下搅拌至溶液混合均匀,转速为200～1000r/min；往溶液中滴加质量百分比为10％的冰醋酸溶液,缓慢调节溶液的pH值直至在显微镜下观察溶液中出现球形微胶囊,停止加热,冷却,滴加固化剂溶液,反应30～60min,静置离心,去掉上层清液,沉淀物冻干即为明胶/CMC病毒微胶囊。本发明的明胶/CMC病毒微胶囊可应用于斜纹夜蛾多角体病毒,安全环保,抗紫外性能强,可以有效保护病毒不被紫外线杀死,提高杀虫率。(The invention discloses a preparation method of gelatin/CMC insect virus microcapsules, which comprises the following steps: (1) dissolving CMC in deionized water to prepare a CMC solution; adding insect virus dry powder into the CMC solution, and stirring and mixing uniformly to form a mixture A; preparing 1-5% gelatin solution by using the ready-prepared buffer solution; (2) adding a gelatin solution into the mixture A, wherein the mass ratio of gelatin to CMC is 1: 2-12: 1; stirring at 25-35 ℃ until the solution is uniformly mixed, wherein the rotating speed is 200-1000 r/min; and (3) dropwise adding a glacial acetic acid solution with the mass percentage of 10% into the solution, slowly adjusting the pH value of the solution until spherical microcapsules appear in the solution under the observation of a microscope, stopping heating, cooling, dropwise adding a curing agent solution, reacting for 30-60 min, standing, centrifuging, removing a supernatant, and freeze-drying the precipitate to obtain the gelatin/CMC virus microcapsules. The gelatin/CMC virus microcapsule of the invention can be applied to prodenia litura polyhedrosis virus, is safe and environment-friendly, has strong uvioresistant performance, can effectively protect the virus from being killed by ultraviolet rays, and improves the insecticidal rate.)

1. A preparation method of gelatin/CMC insect virus microcapsules is characterized by comprising the following steps:

(1) dissolving CMC in deionized water to prepare a CMC solution with the mass fraction of 1%; adding insect virus dry powder into the CMC solution, wherein the mass ratio of the insect virus dry powder to the CMC is 1: 10-2: 1, and uniformly stirring and mixing to form a mixture A; preparing 1-5% gelatin solution by using the ready-prepared buffer solution;

(2) adding a gelatin solution into the mixture A, wherein the mass ratio of gelatin to CMC is 1: 2-12: 1; stirring at 25-35 ℃ until the solution is uniformly mixed, wherein the rotating speed is 200-1000 r/min; and (2) dropwise adding a glacial acetic acid solution with the mass percentage of 10% into the solution, slowly adjusting the pH value of the solution until spherical microcapsules appear in the solution under the observation of a microscope, stopping heating, cooling the solution, adding a curing agent (1% -4% of the dosage of the gelatin and the CMC), reacting for 30-60 min, standing, centrifuging, removing supernatant, and freeze-drying precipitates to obtain the gelatin/CMC virus microcapsules.

2. The method for preparing gelatin/CMC virus microcapsule according to claim 1, wherein the curing agent is formaldehyde, glutaraldehyde, urea aldehyde or tea polyphenol.

3. The method for preparing gelatin/CMC virus microcapsule according to claim 2, wherein the tea polyphenol solution is 1mg/ml to 5mg/ml tea polyphenol aqueous solution.

4. The method for preparing gelatin/CMC virus microcapsule according to claim 2, wherein the formaldehyde, glutaraldehyde, urea aldehyde solution is 0.5mg/ml to 1mg/ml aqueous solution.

5. The method for preparing gelatin/CMC virus microcapsule according to claim 1, wherein the insect virus dry powder is prodenia litura polyhedrosis virus dry powder or beet armyworm polyhedrosis virus dry powder.

6. The method for preparing gelatin/CMC virus microcapsule according to claim 1, wherein the buffer solution is an acetic acid aqueous solution of gelatin, and the pH value is 5.8.

Technical Field

The invention relates to the technical field of slow-release microcapsule pesticides, in particular to a preparation method of gelatin/CMC insect virus microcapsules.

Background

The country is currently dedicated to developing healthy China, advocates green development and attaches great importance to ecological environment protection. Therefore, the traditional pesticide with high toxicity, high pollution, difficult storage, poor physical and chemical stability and low utilization rate can not meet the current requirements of people any more, so that people pay more attention to the research on the slow release and controlled release of the pesticide while researching and inventing a new pesticide product.

The microcapsule technology is a technology that natural or synthetic high molecular material is used as a capsule wall, active substances (capsule cores) are wrapped by a chemical method, a physical method or a physical-chemical method to form a semipermeable or sealed capsule film, and has the advantages that when the microcapsule is formed, the capsule cores are wrapped and isolated from the external environment, the capsule cores can be released after wall breaking under proper conditions, the capsule cores can be prevented from being influenced by factors such as external temperature, oxygen, ultraviolet rays and the like, the application reports of the microcapsule technology in the biological pesticide are few, the microcapsule technology has attracted extensive attention due to the advantages and functions, particularly, the awareness of the society on safety, environment, ecology and sustainable development is continuously enhanced, and when the biological pesticide is continuously developed and applied, the microcapsule becomes an important development direction of the biological pesticide preparation.

The insect virus is continuously researched and promoted in the aspects of market and application as a biological pesticide, is applied to various fields, and has a very wide prospect. Because the environment-friendly paint exists in nature, the paint does not affect the environment and does not pollute the environment. The existing insect virus insecticide has insufficient ultraviolet resistance, so that the application of insect viruses is influenced.

Therefore, there is a need to develop a method for preparing gelatin/CMC virus microcapsules which are safe, environmentally friendly and have strong ultraviolet resistance and are applied to insect virus insecticides.

Disclosure of Invention

In view of the above problems, the present invention provides a method for preparing gelatin/CMC insect virus microcapsules.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of gelatin/CMC insect virus microcapsules comprises the following steps:

(1) dissolving CMC in deionized water to prepare a CMC solution with the mass fraction of 1%; adding insect virus dry powder into the CMC solution, wherein the mass ratio of the insect virus dry powder to the CMC is 1: 10-2: 1, and uniformly stirring and mixing to form a mixture A; preparing 1-5% gelatin solution by using the ready-prepared buffer solution;

(2) adding a gelatin solution into the mixture A, wherein the mass ratio of gelatin to CMC is 1: 2-12: 1; stirring at 25-35 ℃ until the solution is uniformly mixed, wherein the rotating speed is 200-1000 r/min; and (2) dropwise adding a glacial acetic acid solution with the mass percentage of 10% into the solution, slowly adjusting the pH value of the solution until spherical microcapsules appear in the solution under the observation of a microscope, stopping heating, cooling the solution, adding a curing agent (1% -4% of the dosage of the gelatin and the CMC), reacting for 30-60 min, standing, centrifuging, removing supernatant, and freeze-drying precipitates to obtain the gelatin/CMC virus microcapsules.

Preferably, the curing agent is formaldehyde, glutaraldehyde, urea aldehyde or tea polyphenol.

Preferably, the tea polyphenol solution is 1 mg/ml-5 mg/ml tea polyphenol water solution.

Preferably, the formaldehyde, glutaraldehyde and urea-formaldehyde solution is 0.5mg/ml to 1mg/ml of aqueous solution.

Preferably, the virus dry powder is prodenia litura polyhedrosis virus dry powder or beet armyworm polyhedrosis virus dry powder.

Preferably, the buffer solution is an acetic acid aqueous solution of gelatin, and the pH value is 5.8.

Compared with the prior art, the invention has the following beneficial effects:

the gelatin/CMC virus microcapsule provided by the invention can be applied to control insects such as prodenia litura, beet armyworm and the like, is safe and environment-friendly, has strong ultraviolet resistance, can effectively protect insect viruses from being killed by ultraviolet rays, and improves the insecticidal rate.

Drawings

FIG. 1 is a microscope picture of sample 1 in example 1;

FIG. 2 is a microscope picture of microcapsules (blank) prepared using curing agent tea polyphenols;

FIG. 3 is a microscope picture of microcapsules (viruses) prepared using curing agent tea polyphenols;

FIG. 4 is a microscope picture of microcapsules (blanks) prepared using the curing agent formaldehyde;

fig. 5 is a microscope picture of microcapsules (viruses) prepared using curing agent formaldehyde.

Detailed Description

The present invention will be further described with reference to the following examples.

It should be noted that the raw materials used in the technical scheme provided by the present invention, except for the specific description, are prepared by conventional means or purchased through commercial channels.