阅读说明：本技术 一种动物血浆蛋白联产提取工艺 (Process for co-production and extraction of animal plasma proteins ) 是由 许帅辉 赵悦华 赵水 常明利 于 2019-11-04 设计创作，主要内容包括：本发明提供一种动物血浆蛋白联产提取工艺,涉及生物工程技术领域。包括以下步骤：S1.采集动物血浆,将动物血浆置于-4℃的环境下；S2.取适量动物血浆置于分离器中,调节分离器的温度,往血浆中加入乙醇溶液A并将其两者充分搅拌混合,调节混合液的PH值,静置15-30min后,得到沉淀A与上清液A；S3.分离沉淀A与上清液A,将沉淀A中加入乙醇溶液B将其两者充分搅拌混合,除去上清液,即得层粘连蛋白。通过优化提取工艺以及严格控制提取参数,可以从动物血浆中连续化提取出层粘连蛋白、纤连蛋白、球蛋白、珠蛋白与白蛋白,不仅提高了蛋白质的提取效率,节约大量的提取时间,同时也大大提高了蛋白质的纯度。(The invention provides a animal plasma protein co-production extraction process, which relates to the technical field of bioengineering and comprises the following steps of S1, collecting animal plasma, placing the animal plasma in an environment of-4 ℃, S2, placing a proper amount of the animal plasma in a separator, adjusting the temperature of the separator, adding an ethanol solution A into the plasma, fully stirring and mixing the plasma and the ethanol solution A, adjusting the pH value of a mixed solution, standing for 15-30min to obtain a precipitate A and a supernatant A, S3, separating the precipitate A from the supernatant A, adding an ethanol solution B into the precipitate A, fully stirring and mixing the precipitate A and the ethanol solution B, and removing the supernatant to obtain laminin.)

The co-production extraction process of animal plasma proteins is characterized by comprising the following steps:

s1, collecting animal plasma, and placing the animal plasma in an environment at-4 ℃;

s2, placing a proper amount of animal plasma in a separator, adjusting the temperature of the separator, adding the ethanol solution A into the plasma, fully stirring and mixing the ethanol solution A and the plasma, adjusting the pH value of the mixed solution, and standing for 15-30min to obtain a precipitate A and a supernatant A;

s3, separating the precipitate A from the supernatant A, adding an ethanol solution B into the precipitate A, fully stirring and mixing the precipitate A and the ethanol solution B, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain laminin;

s4, placing the supernatant A into another separator, adding an ethanol solution C into the supernatant A, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 20-25min to obtain a precipitate B and a supernatant B, separating the precipitate B from the supernatant B, adding an ethanol solution D into the precipitate B, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain fibronectin;

s5, placing the supernatant B into another separator, adding an ethanol solution E into the supernatant B, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 23-29min to obtain a precipitate C and a supernatant C, separating the precipitate C from the supernatant C, adding an ethanol solution F into the precipitate C, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain globulin;

s6, placing the supernatant C into another separator, adding an ethanol solution G into the supernatant C, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 30-36min to obtain a precipitate D and a supernatant D, separating the precipitate D from the supernatant D, adding an ethanol solution H into the precipitate D, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain the globin;

s7, placing the supernatant D into another separator, adding an ethanol solution J into the supernatant D, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 18-24min to obtain a precipitate E and a supernatant E, separating the precipitate E from the supernatant E, adding an ethanol solution L into the precipitate E, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain the albumin.

2. The co-production extraction process of animal plasma proteins as claimed in claim 1, wherein the temperature of the separators in steps 2-7 is from-10 ℃ to-2 ℃, the stirring speed is 3000-5000r/min, and the stirring time is 1-2 min.

3. The co-production extraction process of animal plasma proteins, according to claim 1, wherein the concentration of ethanol solution A in step 2 is 6-10%, and the pH value is 7-7.5.

4. The co-production extraction process of animal plasma proteins, according to claim 1, wherein the concentration of ethanol solution B in step 3 is 13-18%, and the pH is in the range of 5-7.

5. The co-production extraction process of animal plasma proteins, according to claim 1, wherein the concentration of the ethanol solution C in step 4 is 19-26%, the pH value is 4-6, and the concentration of the ethanol solution D is 35-45%.

6. The co-production extraction process of animal plasma proteins according to claim 1, wherein the concentration of the ethanol solution E in step 5 is 17-25%, the pH value is 6.5-7.5, and the concentration of the ethanol solution F is 35-45%.

7. The co-production extraction process of animal plasma proteins according to claim 1, wherein the concentration of the ethanol solution G in step 6 is 22-29%, the pH value is 6.5-7.5, and the concentration of the ethanol solution H is 35-45%.

8. The co-production extraction process of animal plasma proteins according to claim 1, wherein the concentration of the ethanol solution J in step 7 is 25-30%, the pH value is 7.1-7.3, and the concentration of the ethanol solution L is 35-45%.

Technical Field

The invention relates to the technical field of bioengineering, in particular to a co-production extraction process of animal plasma proteins.

Background

The blood plasma has the main functions of carrying blood cells, transporting substances required for maintaining human life activities, waste products generated in vivo and the like, is equivalent to the intercellular substance of connective tissue, is an important component of blood, is a light yellow liquid (containing bilirubin), contains 90-92% of water in chemical components of the blood plasma, contains solute plasma protein as the main component in other 10%, contains electrolytes, nutrients, enzymes, hormones, cholesterol and other important components, and is a general term of various proteins.

Various proteins in plasma protein play very important roles, for example, albumin plays a main role in forming plasma colloid osmotic pressure and transporting certain small molecular substances and fat-soluble substances, globulin has a close relationship with specific immunity, and fibrin is an important substance participating in a blood coagulation process, at present, various protein extraction modes are numerous, but most extraction methods are complex in process, time consumed in the extraction process is long, large-scale production cannot be achieved, and the purity of the extracted protein needs to be further improved by .

Disclosure of Invention

() problems to be solved

Aiming at the defects of the prior art, the invention provides a co-production extraction process of animal plasma proteins, and solves the problems that the extraction method in the prior art is complex in process, much time is consumed in the extraction process, large-scale production cannot be realized, and the purity of the extracted proteins needs to be improved by steps.

(II) technical scheme

In order to realize the purpose, the invention is realized by the following technical scheme that the co-production extraction process of animal plasma proteins comprises the following steps:

s1, collecting animal plasma, and placing the animal plasma in an environment at-4 ℃;

s2, placing a proper amount of animal plasma in a separator, adjusting the temperature of the separator, adding the ethanol solution A into the plasma, fully stirring and mixing the ethanol solution A and the plasma, adjusting the pH value of the mixed solution, and standing for 15-30min to obtain a precipitate A and a supernatant A;

s3, separating the precipitate A from the supernatant A, adding an ethanol solution B into the precipitate A, fully stirring and mixing the precipitate A and the ethanol solution B, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain laminin;

s4, placing the supernatant A into another separator, adding an ethanol solution C into the supernatant A, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 20-25min to obtain a precipitate B and a supernatant B, separating the precipitate B from the supernatant B, adding an ethanol solution D into the precipitate B, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain fibronectin;

s5, placing the supernatant B into another separator, adding an ethanol solution E into the supernatant B, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 23-29min to obtain a precipitate C and a supernatant C, separating the precipitate C from the supernatant C, adding an ethanol solution F into the precipitate C, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain globulin;

s6, placing the supernatant C into another separator, adding an ethanol solution G into the supernatant C, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 30-36min to obtain a precipitate D and a supernatant D, separating the precipitate D from the supernatant D, adding an ethanol solution H into the precipitate D, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain the globin;

s7, placing the supernatant D into another separator, adding an ethanol solution J into the supernatant D, fully stirring and mixing the two, adjusting the pH value of the mixed solution, standing for 18-24min to obtain a precipitate E and a supernatant E, separating the precipitate E from the supernatant E, adding an ethanol solution L into the precipitate E, adjusting the pH value of the mixed solution, standing for 10-20min, and removing the supernatant to obtain the albumin.

Preferably, the temperature of the separator in the steps 2-7 is 10 ℃ below zero to 2 ℃ below zero, the stirring speed is 3000-.

Preferably, the concentration of the ethanol solution A in the step 2 is 6-10%, and the pH value is 7-7.5.

Preferably, the concentration of the ethanol solution B in the step 3 is 13-18%, and the pH value is in the range of 5-7.

Preferably, the concentration of the ethanol solution C in the step 4 is 19-26%, the pH value is in the range of 4-6, and the concentration of the ethanol solution D is 35-45%.

Preferably, the concentration of the ethanol solution E in the step 5 is 17-25%, the pH value is 6.5-7.5, and the concentration of the ethanol solution F is 35-45%.

Preferably, the concentration of the ethanol solution G in the step 6 is 22-29%, the pH value is 6.5-7.5, and the concentration of the ethanol solution H is 35-45%.

Preferably, the concentration of the ethanol solution J in the step 7 is 25-30%, the pH value ranges from 7.1 to 7.3, and the concentration of the ethanol solution L is 35-45%.

(III) advantageous effects

The invention provides a co-production extraction process of animal plasma proteins, which has the following beneficial effects:

1. the animal plasma protein co-production extraction process can continuously extract laminin, fibronectin, globulin, globin and albumin from animal plasma by optimizing the extraction process and strictly controlling the extraction parameters, thereby not only improving the extraction efficiency of the protein and saving a large amount of extraction time, but also greatly improving the purity of the protein.

2. The animal plasma protein co-production extraction process adopts animal plasma as a raw material, the raw material is simple in acquisition mode, the extraction cost is reduced, and the large-scale production of various proteins can be realized.

Detailed Description

The following embodiments will be described clearly and completely with reference to the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of of the present invention, rather than all embodiments.