阅读说明：本技术 一种降低库存马里兰烟TSNAs含量的储存方法 (Storage method for reducing TSNAs content of Maryland tobacco in stock ) 是由 李炜 马世金 矫海楠 欧亚非 白若石 胡林 杨振民 张秋月 赵澍 梁凯 刘兴余 于 2021-09-08 设计创作，主要内容包括：本发明提供一种降低库存马里兰烟TSNAs含量的储存方法,所述储存方法包括：将气调剂放入马里兰烟叶原料垛中,所述气调剂与所述马里兰烟叶的质量比为1:200～400；控制氧气含量为0.5～2.5％,二氧化碳含量为2～5％,相对湿度为50～65％。本发明提供的仓储方法能在不降低烟叶感官质量,保证烟叶无虫害、霉变发生的前提下,降低库存马里兰烟叶中TSNAs。(The invention provides a storage method for reducing TSNAs content of Maryland tobacco in stock, which comprises the following steps: putting a gas regulator into a Maryland tobacco leaf raw material stack, wherein the mass ratio of the gas regulator to the Maryland tobacco leaves is 1: 200-400; the oxygen content is controlled to be 0.5-2.5%, the carbon dioxide content is controlled to be 2-5%, and the relative humidity is controlled to be 50-65%. The warehousing method provided by the invention can reduce TSNAs in the Maryland tobacco leaves on the premise of not reducing the sensory quality of the tobacco leaves and ensuring that the tobacco leaves are free from insect damage and mildew.)

1. A storage method for reducing TSNAs content of Maryland tobacco in stock is characterized by comprising the following steps: putting a gas regulator into a Maryland tobacco leaf raw material stack, wherein the mass ratio of the gas regulator to the Maryland tobacco leaves is 1: 200-400; the oxygen content is controlled to be 0.5-2.5%, the carbon dioxide content is controlled to be 2-5%, and the relative humidity is controlled to be 50-65%.

2. The storage method for reducing TSNAs content of Maryland tobacco in stock according to claim 1, wherein the oxygen content is 1-2%, the carbon dioxide content is 3-5%, the relative humidity is 55-65%, and the temperature is 3.2-32.1 ℃.

3. The storage method for reducing TSNAs content in maryland tobacco stock according to claim 1, wherein the gas regulator comprises the following components by mass: 10-90% of oxygen absorbent, 1-30% of humectant, 0.1-20% of cathode material, 0.1-10% of retardant, 0.1-30% of salt and 1-30% of water.

4. The storage method for reducing TSNAs content in maryland tobacco in stock according to claim 3, wherein the oxygen absorbent is pyrite powder or reduced iron powder; and/or the humectant is perlite powder or bentonite; and/or the cathode material is selected from one or more of carbon powder, copper salt and lead salt; and/or the retardant is sulfite, ascorbic acid or hydroxylamine hydrochloride; and/or the salt is a chloride or sulfate.

5. The storage method for reducing TSNAs content of Maryland tobacco in stock according to claim 4, wherein the gas regulator comprises the following components by mass: 20-75% of oxygen absorbent, 5-20% of humectant, 1-5% of cathode material, 0.5-2% of retardant, 1-2% of salt and the balance of water; preferably 65% of pyrite powder, 15% of bentonite, 3% of carbon powder, 1% of ascorbic acid, 1.5% of sodium chloride and the balance of water.

6. The storage method for reducing the TSNAs content of Maryland tobacco in stock according to claim 1, wherein the mass ratio of the air conditioner to the Maryland tobacco leaves is 1: 250-375.

7. The storage method for reducing the TSNAs content of Maryland tobacco in stock according to claim 1, wherein the Maryland tobacco leaves are stacked into stacks with the length, the width and the height being 10-20: 2-5; preferably, the method further comprises sealing the stack with a composite plastic film and extracting air from the stack.

8. The method of reducing TSNAs levels in maryland tobacco inventory of claim 7, wherein said air conditioners are placed around and at the top of said stacks.

9. A storage method for reducing TSNAs levels of Maryland tobacco in stock according to any one of claims 1 to 8, wherein the Maryland tobacco leaf is Maryland tobacco lamina which has been stored for at least 3 years.

10. Use of a storage method for reducing TSNAs content of Maryland tobacco in stock according to any one of claims 1 to 9, wherein the use is for reducing TSNAs content of Maryland tobacco in stock, improving sensory quality of tobacco; preferably, the TSNAs comprises: n-nitrosonornicotine, 4- (N-methylnitrosamine) -1- (3-pyridyl) -1-butanone, N-nitrosoanatabine and N-nitrosoanabasine.

Technical Field

The invention relates to the field of tobacco mellowing, in particular to a storage method for reducing TSNAs content of Maryland tobacco in stock.

Background

Maryland tobacco belongs to light air-cured tobacco, has higher nicotine and total nitrogen content than flue-cured tobacco, lower sugar content, thinner leaves, strong elasticity, high filling force, strong smoldering fire protection power, mellow fragrance, large strength, good absorption capacity, convenient feeding in rolling and is a high-quality raw material for preparing Chinese style blended cigarettes. Alcoholization is one of the essential links for improving the quality of tobacco leaves, and can improve the problems of coarse aroma, heavy local miscellaneous gas, great irritation and the like in new cigarettes, thereby being beneficial to the use of cigarettes. For flue-cured tobacco, natural alcoholization generally needs about 2 years, and external factors such as temperature, humidity, air, light and the like have close relation to alcoholization period and quality. In the case of Maryland tobacco, in practical industrial applications, the alcoholization period is longer than that of flue-cured tobacco, at least for more than 3 years (the actual majority is concentrated on about 5 years).

Some harmful ingredients in tobacco leaves and smoke, particularly tobacco-specific nitrosamines (TSNAs) with carcinogenicity, are always important concerns in the field, and the TSNAs content in the buccal tobacco is taken as an important assessment index for market admission. Tobacco-Specific nitrosamines (TSNAs) are mainly 4 kinds of N-nitrosonornicotine (NNN), 4- (N-methylnitrosamine) -1- (3-pyridyl) -1-butanone (NNK), N-Nitrosoanabasine (NAT) and N-Nitrosoanabasine (NAB). The tobacco leaves before modulation do not contain TSNAs or have extremely low content, and the TSNAs in the tobacco leaves are mainly formed and accumulated in the modulation and storage processes. Due to the influence of the biological characteristics and the modulation mode of Maryland tobacco, the content of harmful substance TSNAs is higher in the Maryland tobacco. Studies have shown that TSNAs levels increase gradually with increasing maryland tobacco storage time. The longer the storage time, the higher the TSNAs content in the tobacco leaves. Aiming at the Maryland tobacco lamina stored in the stock for more than 3 years, if a proper storage mode can be explored, the TSNAs content can be reduced on the premise of not reducing the sensory quality of the tobacco leaves and ensuring that the tobacco leaves are free from insect damage and mildew, and the method has important significance.

In view of this, the present invention is specifically proposed to reduce the TSNAs content in Maryland tobacco leaves.

Disclosure of Invention

The embodiment of the invention provides a warehousing method for reducing TSNAs in Maryland tobacco leaves in stock. The warehousing method provided by the invention can reduce TSNAs in the Maryland tobacco leaves on the premise of not reducing the sensory quality of the tobacco leaves and ensuring that the tobacco leaves are free from insect damage and mildew.

The embodiment of the invention provides a storage method for reducing TSNAs content of Maryland tobacco in stock, which comprises the following steps: putting a gas regulator into a Maryland tobacco leaf raw material stack, wherein the mass ratio of the gas regulator to the Maryland tobacco leaves is 1: 200-400; the oxygen content is controlled to be 0.5-2.5%, the carbon dioxide content is controlled to be 2-5%, and the relative humidity is controlled to be 50-65%. The method provided by the invention is safe and convenient in operation process, can reduce the TSNAs content in the stored Maryland tobacco, does not bring harm to operators and environment, ensures no insect pest and mildew in the storage process of the tobacco leaves, and has a certain positive effect on sensory quality.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in stock, provided by the embodiment of the invention, the method comprises the following steps: the content of oxygen is 1-2%, the content of carbon dioxide is 3-5%, the relative humidity is 55-65%, and the temperature is 3.2-32.1 ℃. According to the invention, by adopting the indexes, the TSNAs content in the Maryland tobacco in stock can be better reduced, and the sensory quality is improved.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in stock, provided by the embodiment of the invention, the gas regulator comprises the following components in percentage by mass: 10-90% of oxygen absorbent, 1-30% of humectant, 0.1-20% of cathode material, 0.1-10% of retardant, 0.1-30% of salt and 1-30% of water. In order to solve the problem that the longer the storage time is, the higher the TSNAs content in the tobacco leaves is, the invention uses the air regulator to control the oxygen content, stabilize the carbon dioxide and the relative humidity content and reduce the nitrosation reaction of alkaloid to reduce the TSNAs production.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in the stock, provided by the embodiment of the invention, the oxygen absorbent is pyrite powder or reduced iron powder; and/or the humectant is perlite powder or bentonite; and/or the cathode material is selected from one or more of carbon powder, copper salt and lead salt; and/or the retardant is sulfite, ascorbic acid or hydroxylamine hydrochloride; and/or the salt is a chloride or sulfate.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in stock, provided by the embodiment of the invention, the gas regulator comprises the following components in percentage by mass: 20-75% of oxygen absorbent, 5-20% of humectant, 1-5% of cathode material, 0.5-2% of retardant, 1-2% of salt and the balance of water; preferably 65% of pyrite powder, 15% of bentonite, 3% of carbon powder, 1% of ascorbic acid, 1.5% of sodium chloride and the balance of water. The granularity of the pyrite powder is 55-65 meshes, and is preferably 55 meshes. In the present invention, by using the above-mentioned particularly preferred ingredients, the amounts of the respective ingredients and the parameters of the gas regulator, the TSNAs content in the stored maryland tobacco can be further reduced, and the positive effect of the sensory quality can be enhanced.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in the stock, provided by the embodiment of the invention, the mass ratio of the air conditioner to the Maryland tobacco leaves is 1: 250-375. In the invention, the quality ratio range is adopted, so that the content of TSNAs of the Maryland tobacco in the stock is reduced, and the sensory quality effect is better.

According to the storage method for reducing TSNAs content of Maryland tobacco in stock, provided by the embodiment of the invention, Maryland tobacco leaves are stacked into a stack body with the length and the width being 10-20: 2-5; preferably, the method further comprises sealing the stack with a composite plastic film and extracting air from the stack.

According to the storage method for reducing the TSNAs content of the Maryland tobacco in stock, provided by the embodiment of the invention, the air regulator is placed at the periphery and the top of the stack body.

According to the storage method for reducing TSNAs content of Maryland tobacco in stock, provided by the embodiment of the invention, Maryland tobacco leaves are Maryland tobacco strips which have been stored for at least 3 years.

The embodiment of the invention also provides a gas regulator used in the storage method for reducing the TSNAs content of the Maryland tobacco in stock.

The embodiment of the invention also provides an application of the storage method for reducing the TSNAs content of the Maryland tobacco in stock, and the storage method is applied to reducing the TSNAs content of the Maryland tobacco in stock and improving the sensory quality of tobacco leaves; preferably, the TSNAs comprises: n-nitrosonornicotine, 4- (N-methylnitrosamine) -1- (3-pyridyl) -1-butanone, N-nitrosoanatabine and N-nitrosoanabasine. The present inventors have found that the storage method has the above-mentioned unexpected application effect.

The invention has the beneficial effects that: the storage method has the advantages of safe and convenient operation process, reduction of TSNAs content in the stored Maryland tobacco, no harm to operators and environment, no insect damage and mildew in the storage process of the tobacco leaves and certain positive effect on sensory quality.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.

FIG. 1 is a graph comparing TSNAs content after one year storage of an embodiment of the present invention and conventional processing;

FIG. 2 is a graph comparing TSNAs content after two years storage of examples of the present invention and conventional processing.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described clearly and completely below, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The instruments and the like are conventional products which are purchased by normal distributors and are not indicated by manufacturers. The process is conventional unless otherwise specified, and the starting materials are commercially available from the open literature. The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications.

In the following examples of the present invention, Maryland tobacco leaves were produced in a product warehouse of the Tokyo redrying factory of Shandong redrying Co., Ltd in 2016 (3 months in 2013) by using Maryland tobacco leaf stock (grade name: 2012-Hubei Yichang MA-S, production date: 2012). The location of the warehouse belongs to semi-humid climate of continental seasonal regions in warm temperature zone, the average temperature in the warehouse is 16.84 ℃, and the relative humidity is 57.13%.

Example 1

The embodiment provides a storage method for reducing TSNAs content of Maryland tobacco in stock, which comprises the following steps:

s1, selecting Maryland tobacco leaves 780 boxes, stacking 4 stacks with the length, width and height being 16m 3m 2.9m, namely each stack is 195 boxes, and the mass of the Maryland tobacco leaves of each stack is 29250 Kg; the bottom of each stack body is pre-padded with a composite plastic film;

s2, adding 95kg of air conditioner into each stack body, uniformly placing the air conditioner around and at the top of the stack body, sealing the stack body by using a composite plastic film, and extracting air in the stack body; the air regulating agent comprises the following components in percentage by mass: 65% of pyrite powder, 15% of bentonite, 3% of carbon powder, 1% of ascorbic acid, 1.5% of sodium chloride and the balance of water.

S3: monitoring relative humidity, temperature, oxygen and carbon dioxide concentration every day, controlling the oxygen concentration to be 1-2% by slightly regulating and controlling an air valve after the oxygen concentration is reduced to be below 1%, and injecting the carbon dioxide into a carbon dioxide gas storage tank after the carbon dioxide concentration is lower than 3% so as to keep the oxygen concentration to be 3-5%; the relative humidity is controlled to be 55-65% and the temperature is controlled to be 3.2-32.1 ℃.

S4, taking the oxygen concentration as a time point, and sampling and evaluating Maryland tobacco of 4 piles every 6 months;

performing TSNAs detection on Maryland tobacco by using a high performance liquid chromatography-triple quadrupole tandem mass spectrometer; evaluating the sensory quality of Maryland tobacco by professional smokers, and detecting and evaluating the content of the Maryland tobacco in the following comparative example 1 to be consistent; TSNAs detection method: adopting an online SPE-liquid chromatography-mass spectrometry (SPELC-MS/MS) method, namely fully-automatically extracting a solid phase of a tobacco sample, and detecting the extract by using LC-MS/MS after the extract passes through a water phase filter membrane; the sensory quality evaluation adopts 100-point system: 15 minutes of fragrance quality, 15 minutes of fragrance quantity, 10 minutes of miscellaneous gas, 10 minutes of concentration, 10 minutes of irritation, 15 minutes of aftertaste, 15 minutes of strong coordination, 15 minutes of combustibility and gray color, and 100 minutes in total.

Comparative example 1

The comparative example provides a warehouse maintenance method, and the same batch of Maryland finished tobacco lamina 195 boxes are alternatively taken, the total amount is 29250Kg, the Maryland finished tobacco lamina is placed in the same warehouse as the example 1, and the difference with the example 1 is that: 1) air-conditioning sealing treatment is not adopted; 2) the relative humidity in the tobacco leaf storage process is the natural state in the warehouse: 45-65%, oxygen content of about 21%, carbon dioxide concentration of about 0.03%; 3) aluminum phosphide is adopted to fumigate and kill the tobacco leaves in the batch every 4 months and 9 months.

Comparative example 2

The comparative example provides a warehouse maintenance method, alternatively takes 195 boxes of the Maryland finished product tobacco sheets of the same batch, totals 29250Kg, places the Maryland finished product tobacco sheets in the same warehouse as the example 1, adopts the same type of air conditioner and treatment, and maintains the same carbon dioxide concentration and relative humidity as the example 1; the difference from example 1 is that: controlling the oxygen content to be about 6-8%.

Comparative example 3

The comparative example provides a warehouse maintenance method, alternatively takes 195 boxes of the Maryland finished product tobacco sheets of the same batch, totals 29250Kg, places the Maryland finished product tobacco sheets in the same warehouse as the example 1, adopts the same type of air conditioner and treatment, and maintains the same carbon dioxide concentration and relative humidity as the example 1; the difference from example 1 is that: controlling the oxygen content to be about 12-15%.

The sensory quality of the tobacco leaves stored in example 1 of the present invention is compared with those of comparative example 1 (conventional storage method), comparative example 2 and comparative example 3 in Table 1, the TSNAs content is compared with those of comparative example 2, and the TSNAs treated conventionally in example 1 of the present invention is compared with those of comparative example 1 in FIGS. 1 and 2.

Table 1 comparison of sensory quality of the present invention and comparative examples 1 and 2 at different time periods

TABLE 2 TSNAs content comparison of the present invention and comparative examples at different periods of time

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.