阅读说明：本技术 一种桑黄提取物在制备抗癌药物中的应用 (Application of phellinus igniarius extract in preparation of anti-cancer drugs ) 是由 李艳 王家东 耿立 龚守富 杨奋 于 2019-12-13 设计创作，主要内容包括：本发明所述的一种桑黄提取物在治疗肝癌药物中的应用,将桑黄多糖、桑黄总黄酮和桑黄总三萜作为提取物,并探究了桑黄多糖、桑黄总黄酮和桑黄总三萜三种提取物的最佳提取方法,将桑黄多糖、桑黄总黄酮和桑黄总三萜三种提取物应用到药物中,能够活化人体免疫系统,能够配合手术、放、化疗患者提高免疫力,提早伤口愈合,减轻放、化疗毒性,提高红细胞、白细胞的数量,减小癌症复发、癌细胞转移的风险,同时能够减少并发症,且对人体无毒无害,即使长期大剂量服用亦无任何毒副作用；同时制备方法简单易行,可制成多种类型的药物,以方便食用,操作方便,提取效率高,产品质量稳定。(The phellinus igniarius extract is applied to the medicines, so that the human immune system can be activated, the immunity of patients can be improved by matching with operations, radiotherapy and chemotherapy, the wound can be healed in advance, the toxicity of radiotherapy and chemotherapy can be relieved, the number of red blood cells and white blood cells can be increased, the risk of cancer relapse and cancer cell transfer can be reduced, the complications can be reduced, and the phellinus igniarius extract is non-toxic and harmless to a human body and has no toxic or side effect even if the phellinus igniarius extract is taken in a large dose for a long time; meanwhile, the preparation method is simple and feasible, can be used for preparing various types of medicines for eating conveniently, and has convenient operation, high extraction efficiency and stable product quality.)

1. An application of phellinus igniarius extract in preparing anticancer drugs is characterized in that: the Phellinus linteus extract comprises Phellinus linteus polysaccharide, Phellinus linteus total flavone and Phellinus linteus total triterpene.

2. The use of the Phellinus linteus extract as claimed in claim 1 for preparing anticancer drugs, wherein: the medicament can be tablets, granules, pills, capsules, oral liquid, syrup, freeze-dried powder injection or water injection.

3. The use of the Phellinus linteus extract as claimed in claim 1 for preparing anticancer drugs, wherein the Phellinus linteus polysaccharide in the Phellinus linteus extract is extracted by the following steps: crushing the dried phellinus igniarius by using a crusher, screening the crushed phellinus igniarius by using a 40-mesh screen, putting the crushed phellinus igniarius into a high-energy nano impact mill, crushing the phellinus igniarius to obtain 500-nano phellinus igniarius nano powder with the average particle size of 100, then leaching the phellinus igniarius nano powder for 30-90 min at the temperature of 80-100 ℃ by using water with the weight 15-20 times of the weight of the phellinus igniarius, cooling the phellinu.

4. The use of the Phellinus linteus extract as claimed in claim 1 for preparing anticancer drugs, wherein the Phellinus linteus total flavonoids in the Phellinus linteus extract are extracted by the following steps:

step one, weighing phellinus igniarius sporophore powder with the particle size of 60 meshes, then adding an ethanol solution with the concentration of 60-80%, soaking for 12 hours at room temperature, and extracting by adopting an ultrasonic extraction method to obtain a primary extracting solution;

step two, adding 60-80% ethanol solution into the extraction residue left in the step one, and extracting once again by adopting an ultrasonic extraction method to obtain secondary extracting solution;

and step three, combining the primary extract and the secondary extract, recovering ethanol, and freeze-drying the concentrated solution to obtain the phellinus igniarius total flavonoids.

5. The use of Phellinus Linteus extract in preparing anticancer drugs according to claim 4, wherein the ethanol solution is added in a ratio of 1:30 (g/ml) in both of the first and second steps.

6. The use of Phellinus Linteus extract in preparing anticancer drugs as claimed in claim 4, wherein in the first and second steps, the temperature of ultrasonic extraction is 40-50 deg.C, and the extraction time is 40-50 min.

7. The use of the phellinus igniarius extract in the preparation of an anticancer drug according to claim 1, wherein the phellinus igniarius extract is obtained by the following extraction method of the phellinus igniarius total triterpenes:

step one, grinding dried phellinus igniarius mycelium into powder, adding 60-80% ethanol solution into the powder, wherein the material-liquid ratio of phellinus igniarius mycelium powder to ethanol solution is 1: 20 (g/ml), extracting by an ultrasonic extraction method, filtering, and taking supernatant to prepare primary leaching liquor;

step two, adding 60-80% ethanol solution into the extraction residue left in the step one, wherein the material-liquid ratio of the extraction residue to the ethanol solution is 1: 20 (g/ml), and extracting once again by adopting an ultrasonic extraction method to obtain a secondary leaching liquor;

and step three, combining the primary extract and the secondary extract, and recovering ethanol to obtain the phellinus igniarius total triterpenes.

8. The use of the Phellinus linteus extract as claimed in claim 7 for preparing anticancer drugs, wherein: in the first step and the second step, the temperature of ultrasonic extraction is 55-65 ℃, and the extraction time is 21 min.

Technical Field

The invention relates to the technical field of medicines, in particular to application of a phellinus igniarius extract in preparation of an anti-cancer medicine.

Background

Phellinus linteus, the primordial source of which is the fruiting body of Phellinus linteus of Polyporaceae, also known as: phellinus igniarius, fruitbody without stalk, pileus flat hemispherical or horseshoe shape, wood, light liver brown to dark gray or black, old chap, no skin shell, fine villi in the initial stage, no hair after change, concentric ring edges, blunt edges, dark cinnamon color to light coffee color, and no seed layer on the lower side. The mushroom flesh is dark brown, hard and woody. The tube is similar to the meat, but has multiple layers, and the old tube is filled with white hypha. Rusty brown to dark brown, round, nearly spherical, smooth, colorless. The bristle tip is sharp and the base is enlarged. Hyphae did not branch and did not have transverse septa. Is originated from trunk of poplar, willow, birch, oak, etc. Distributed in northeast, northwest, Sichuan and Yunnan provinces. Phellinus igniarius has effects of benefiting five internal organs, softening hard masses, removing toxic substances, stopping bleeding, promoting blood circulation, regulating stomach and stopping diarrhea, and is mainly used for treating gonorrhea, metrorrhagia, leukorrhagia, abdominal mass accumulation, retained fluid, spleen deficiency and diarrhea.

Modern researches have proved that phellinus igniarius contains effective components such as flavone and polysaccharide and has pharmacological activities in various aspects such as immunoregulation, antitumor and anticancer, so that it is necessary to search and extract highly active anticancer active sites from phellinus igniarius.

Disclosure of Invention

In order to solve the problems, the invention provides an application of phellinus igniarius extract in preparing anticancer drugs.

The technical scheme adopted by the invention to solve the problems is as follows: an application of a phellinus igniarius extract in preparing an anticancer drug is disclosed, wherein the phellinus igniarius extract comprises phellinus igniarius polysaccharides, phellinus igniarius total flavonoids and phellinus igniarius total triterpenes.

In the invention, the medicament dosage form is tablets, granules, pills, capsules, oral liquid, syrup, freeze-dried powder injection or water injection.

In the invention, the extraction method of phellinus igniarius polysaccharides in the phellinus igniarius extract comprises the following steps: crushing the dried phellinus igniarius by using a crusher, screening the crushed phellinus igniarius by using a 40-mesh screen, putting the crushed phellinus igniarius into a high-energy nano impact mill, crushing the phellinus igniarius to obtain 500-nano phellinus igniarius nano powder with the average particle size of 100, then leaching the phellinus igniarius nano powder for 30-90 min at the temperature of 80-100 ℃ by using water with the weight 15-20 times of the weight of the phellinus igniarius, cooling the phellinu.

In the invention, the method for extracting the total flavonoids of phellinus igniarius in the phellinus igniarius extract comprises the following steps:

step one, weighing phellinus igniarius sporophore powder with the particle size of 60 meshes, then adding an ethanol solution with the concentration of 60-80%, soaking for 12 hours at room temperature, and extracting by adopting an ultrasonic extraction method to obtain a primary extracting solution;

step two, adding 60-80% ethanol solution into the extraction residue left in the step one, and extracting once again by adopting an ultrasonic extraction method to obtain secondary extracting solution;

and step three, combining the primary extract and the secondary extract, recovering ethanol, and freeze-drying the concentrated solution to obtain the phellinus igniarius total flavonoids.

In the invention, in the first step and the second step, the ethanol solution is added according to the feed-liquid ratio of 1:30 (g/ml).

In the invention, in the first step and the second step, the temperature of ultrasonic extraction is 40-50 ℃, and the extraction time is 40-50 min.

In the invention, the method for extracting the total triterpenoids in the phellinus igniarius extract comprises the following steps:

step one, grinding dried phellinus igniarius mycelium into powder, adding 60-80% ethanol solution into the powder, wherein the material-liquid ratio of phellinus igniarius mycelium powder to ethanol solution is 1: 20 (g/ml), extracting by an ultrasonic extraction method, filtering, and taking supernatant to prepare primary leaching liquor;

step two, adding 60-80% ethanol solution into the extraction residue left in the step one, wherein the material-liquid ratio of the extraction residue to the ethanol solution is 1: 20 (g/ml), and extracting once again by adopting an ultrasonic extraction method to obtain a secondary leaching liquor;

and step three, combining the primary extract and the secondary extract, and recovering ethanol to obtain the phellinus igniarius total triterpenes.

In the invention, in the first step and the second step, the temperature of ultrasonic extraction is 55-65 ℃, and the extraction time is 21 min.

Compared with the prior art, the invention has the following beneficial effects:

the invention relates to an application of a phellinus igniarius extract in preparing anticancer drugs, which takes phellinus igniarius polysaccharide, phellinus igniarius total flavone and phellinus igniarius total triterpene as extracts, researches the optimal extraction method of the three extracts of the phellinus igniarius polysaccharide, the phellinus igniarius total flavone and the phellinus igniarius total triterpene, applies the three extracts of the phellinus igniarius polysaccharide, the phellinus igniarius total flavone and the phellinus igniarius total triterpene to drugs, can activate the immune system of a human body, can be matched with patients suffering from operation, radiotherapy and chemotherapy to improve the immunity, heal wounds in advance, lighten the toxicity of radiotherapy and chemotherapy, improve the number of red blood cells and white blood cells, reduce the risks of cancer relapse and cancer cell transfer, can reduce complications, is non-toxic and harmless to the; meanwhile, the preparation method is simple and feasible, can be used for preparing various types of medicines for eating conveniently, and has convenient operation, high extraction efficiency and stable product quality.

Detailed Description

The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.