Method and device for preparing hydrogen-rich water by using photosynthetic microalgae
阅读说明：本技术 一种利用光合微藻制备富氢水的方法及装置 (Method and device for preparing hydrogen-rich water by using photosynthetic microalgae ) 是由 陈梅 于 2019-09-24 设计创作，主要内容包括：本发明提供一种利用光合微藻制备富氢水的方法及装置。本发明利用微藻将太阳光转化为氢气,并通过排水法实时将氢气通入无菌生理盐水中,利用微藻产生氢气本身的压力将其溶解于生理盐水中,排出的生理盐水即为富氢水,同时收集纯度极高的氢气气体。此方法相比较传统富氢水制备方法具有清洁高效安全的特点。(The invention provides a method and a device for preparing hydrogen-rich water by using photosynthetic microalgae. The invention utilizes microalgae to convert sunlight into hydrogen, feeds the hydrogen into sterile physiological saline in real time through a drainage method, dissolves the hydrogen into the physiological saline by utilizing the pressure of the hydrogen generated by the microalgae, discharges the physiological saline which is hydrogen-rich water, and collects hydrogen gas with extremely high purity. Compared with the traditional hydrogen-rich water preparation method, the method has the characteristics of cleanness, high efficiency and safety.)
1. The utility model provides a device of preparation hydrogen-rich water, includes little algae photosynthetic hydrogen production system, discharges water and receives gas system and hydrogen-rich water collecting system, little algae photosynthetic hydrogen production system, discharge water and receive gas system and hydrogen-rich water collecting system pass through aseptic pipe sequence connection.
2. The apparatus of claim 1, wherein: the microalgae photosynthetic hydrogen production system comprises a microalgae culture bottle,
a microalgae culture system consisting of photosynthetic microalgae cell suspension and a sulfur-deficient TAP culture solution is placed in the microalgae culture bottle.
3. The apparatus of claim 1 or 2, wherein: the microalgae photosynthetic hydrogen production system also comprises a group of LED lamps, a magnetic stirrer, a solar panel and a storage battery;
the solar panel is used for converting solar energy into electric energy to be stored in the storage battery;
the storage battery is used for supplying power to the LED lamp and the magnetic stirrer so as to support the photosynthetic hydrogen production of the microalgae.
4. The apparatus of any one of claims 1-3, wherein: the mouth of the microalgae culture bottle is sealed by a rubber stopper with a sterile catheter;
the drainage and gas collection system is an aseptic bottle filled with sterilized normal saline;
the hydrogen-rich water collection system is a vacuum aseptic bag;
the microalgae photosynthetic hydrogen production system is connected with one end of the sterile bottle filled with the sterilized normal saline through a sterile catheter arranged on the rubber plug, and the other end of the sterile bottle filled with the sterilized normal saline is connected with the vacuum sterile bag;
the aseptic guide pipe connecting the microalgae photosynthetic hydrogen production system, the water drainage and gas collection system and the hydrogen-rich water collection system is provided with a controllable valve.
5. A method for preparing hydrogen-rich water using photosynthetic microalgae, comprising:
1) adding photosynthetic microalgae cell suspension and sulfur-deficient TAP culture solution into a microalgae culture bottle to obtain a microalgae culture system,
2) sealing the mouth of a culture bottle by using a rubber stopper with a sterile catheter, connecting the sterile catheter to one end of a sterile bottle filled with sterilized normal saline, and connecting the other end of a sterile glass bottle filled with sterilized normal saline with a vacuum sterile bag;
3) starting the device to perform continuous illumination culture;
4) the method comprises the steps of introducing generated hydrogen into sterile physiological saline in real time through a drainage method during illumination culture, dissolving the hydrogen into the physiological saline by utilizing the pressure of the hydrogen generated by microalgae, wherein the discharged physiological saline is hydrogen-rich water, and meanwhile, collecting high-purity hydrogen gas.
6. The method of claim 5, wherein: in the step 1), the photosynthetic microalgae is Chlamydomonas reinhardtii.
7. The method of claim 5, wherein: the photosynthetic microalgae cell suspension is prepared by a method comprising the following steps:
a) selecting photosynthetic microalgae cells cultured by a flat plate within two weeks, transferring the photosynthetic microalgae cells into TAP culture solution, and performing shake culture to obtain pre-cultured photosynthetic microalgae cells;
b) inoculating the pre-cultured photosynthetic microalgae cells into TAP culture solution, and culturing for 24-36 h;
c) suspending the cells obtained in the step b) by using a sulfur-deficient TAP culture solution to obtain a photosynthetic microalgae cell suspension.
8. The method of claim 5, wherein: in the microalgae culture system obtained in the step 1), the initial chlorophyll concentration of photosynthetic microalgae cells is 25 mug/mL.
9. The method of claim 5, wherein: in the step 3), the illumination intensity of the continuous illumination culture is as follows: 100 muEs-1m-2At a temperature of 25 ℃;
the continuous illumination culture time is more than 30 hours.
Technical Field
The invention belongs to the technical field of new energy and biological hydrogen production, and particularly relates to a method and a device for preparing hydrogen-rich water by using photosynthetic microalgae.
Background
Hydrogen molecules are the smallest, simplest molecules of nature. The gel has extremely strong penetrability, is easy to penetrate skin and mucous membrane to enter cells, cell nucleuses and mitochondria, and is easy to be absorbed and utilized by human bodies. Hydrogen has an antioxidant effect, can efficiently eliminate malignant free radicals, maintain the internal environment balance of intracellular and extracellular body fluids, and maintain effective immunity and self-repairing capability [ Ohsawa I, Ishikawa M, Takahashi K, et al. hydrogen acts a therapeutic antioxidant substrate reducing cytoxic oxidative chemicals [ J ]. Nature Medicine,2007,13(6):688 ]; but also can improve diseases, improve allergic constitution and play a role in good weight-losing effect [ Yuguangyu, hydrogen of beautiful skin [ G ], "Zhengzhou": the world college of health preserving and beauty academy of traditional Chinese medicine, 2015 ]; simultaneously, the blood viscosity and the blood pressure can be reduced, and diabetes [ Kajiyama S, Hasegawa G, Asano M, et al.supplement of water-rich water improvements lipid and glucose metabolism in tissues with type 2diabetes improved glucose tolerance [ J ]. Nutr Res,2008,28: 137-; nakao A, Toyoda Y, Sharma P, Evans M, Guthrie N.Effect of hydrogen rich water antibiotic status of subjects with potential metallic synthetic-an open label pillow [ J ]. J Clin Biochem Nutr,2010,46: 140-; song G, Li M, Sangg H, et al.Hydrogen-rich water depletion serum LDL-cholesterol levels and improved HDL functions in a batch with a potential metal syndrome [ J ]. J Lipid Res,2013,54: 1884-. Meanwhile, the hydrogen can relieve the injury of small intestine, liver and genital cells caused by radiation, and has obvious protection effect on visceral organs.
Because hydrogen is inflammable and explosive, the preparation, storage and use conditions of hydrogen are limited. The current ideal method is to dissolve high-purity industrial hydrogen in water or physiological saline for more than 4 hours under a certain pressure (0.4-0.6MPa) to prepare hydrogen-rich saturated liquid [6], which can be orally applied, but causes a great deal of waste of hydrogen and has great potential safety hazard. And in the process of storing the hydrogen-rich water, the hydrogen concentration is gradually reduced along with the prolonging of the standing time, and the change of the hydrogen concentration has no obvious correlation with the storage container and the storage temperature [ Qinxu, safety, Zhang Wei, and the like.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, the present invention provides an apparatus for preparing hydrogen-rich water and a method for preparing hydrogen-rich water using the same.
The device for preparing the hydrogen-rich water comprises a microalgae photosynthetic hydrogen production system, a water discharging gas collecting system and a hydrogen-rich water collecting system, wherein the microalgae photosynthetic hydrogen production system, the water discharging gas collecting system and the hydrogen-rich water collecting system are sequentially connected through a sterile conduit;
the microalgae photosynthetic hydrogen production system comprises a microalgae culture bottle,
a microalgae culture system consisting of photosynthetic microalgae cell suspension and a sulfur-deficient TAP culture solution is placed in the microalgae culture bottle;
the microalgae photosynthetic hydrogen production system can further comprise a group of LED lamps, a magnetic stirrer, a solar panel and a storage battery;
the solar panel is used for converting solar energy into electric energy to be stored in the storage battery;
the storage battery is used for supplying power to the LED lamp and the magnetic stirrer so as to support the photosynthetic hydrogen production of the microalgae;
the mouth of the microalgae culture bottle is sealed by a rubber stopper with a sterile catheter;
the drainage and gas collection system is an aseptic bottle filled with sterilized normal saline;
the hydrogen-rich water collection system is a vacuum aseptic bag;
the microalgae photosynthetic hydrogen production system is connected with one end of the sterile bottle filled with the sterilized normal saline through a sterile catheter arranged on the rubber plug, and the other end of the sterile bottle filled with the sterilized normal saline is connected with the vacuum sterile bag;
the aseptic guide pipe connecting the microalgae photosynthetic hydrogen production system, the water drainage and gas collection system and the hydrogen-rich water collection system is provided with a controllable valve.
The invention also provides a method for preparing hydrogen-rich water by using the photosynthetic microalgae.
The method for preparing hydrogen-rich water by using photosynthetic microalgae comprises the following steps:
1) firstly, constructing the device for preparing the hydrogen-rich water according to the connection relationship of all components of the device for preparing the hydrogen-rich water, then adding a microalgae culture system consisting of photosynthetic microalgae cell suspension and TAP (sulfur deficiency) culture solution into a microalgae culture bottle of the microalgae photosynthetic hydrogen production system,
2) sealing the mouth of a culture bottle by using a rubber stopper with a sterile conduit, connecting the sterile conduit to one end of a sterile bottle (namely a drainage and air-receiving system) filled with sterilized normal saline, and connecting the other end of the sterile glass bottle filled with sterilized normal saline with a vacuum sterile bag (namely a hydrogen-rich water collection system);
3) starting the device to perform continuous illumination culture;
4) the method comprises the steps of introducing generated hydrogen into sterile physiological saline in real time through a drainage method during illumination culture, dissolving the hydrogen into the physiological saline by utilizing the pressure of the hydrogen generated by microalgae, wherein the discharged physiological saline is hydrogen-rich water, and meanwhile, collecting high-purity hydrogen gas.
In the above method, the culture bottle may be a Schott culture bottle;
the photosynthetic microalgae may be specifically Chlamydomonas reinhardtii (Chlamydomonas reinhardtii);
the photosynthetic microalgae cell suspension is prepared by a method comprising the following steps:
a) selecting photosynthetic microalgae cells cultured by a flat plate within two weeks, transferring the photosynthetic microalgae cells into TAP culture solution, and performing shake culture to obtain pre-cultured photosynthetic microalgae cells;
b) collecting pre-cultured cells, inoculating the cells into TAP culture solution, and culturing for 24-36 h;
c) suspending the cells obtained in the step b) by using a sulfur-deficient TAP culture solution to obtain a photosynthetic microalgae cell suspension;
in the above method, the composition of TAP culture medium is (g/L): NH (NH)4Cl 0.4；MgSO4·7H2O 0.1；CaC12·2H2O0.05；K2HPO4 0.108；KH2PO40.056; trisbase 2.423; 1mL of Hutner's trace element, 1mL of glacial acetic acid;
the composition of the sulfur-deficient TAP culture solution is (g/L): equimolar conversion of sulfide in TAP culture solution to corresponding chloride;
the solid TAP medium was: adding 1.5% agar powder into TAP culture solution;
Hutner’s trace element(g/L):
in step a), the conditions of shake culture are as follows: 25 ℃, 60rpm, 100. mu.Es-1m-2Performing shake culture for 24-36 h;
in step b), the initial concentration of the pre-cultured photosynthetic microalgal cells in the TAP culture solution can be (1-5) × 105cells/ml。
In the system obtained in the step 1), the initial chlorophyll concentration of the photosynthetic microalgae cells is 25 mug/mL;
in the step 3), the illumination intensity of the continuous illumination culture is as follows: 100 muEs-1m-2At a temperature of 25 ℃;
the continuous illumination culture time is more than 30 hours, specifically 30-120 hours.
The method for preparing hydrogen-rich water by the photosynthetic microalgae drainage method does not need to purchase additional industrial hydrogen and hydrogen production equipment, and the method takes light energy as energy, so that the whole production process is clean and pollution-free; the hydrogen-rich water obtained by the photosynthetic microalgae drainage method is discharged by continuously utilizing the hydrogen pressure generated by the microalgae in the generation process, and the hydrogen molecules are uniformly wrapped by the water molecules, so that the hydrogen and the water are stably combined, the hydrogen content is higher, and the hydrogen content is more stable in the storage process; the photosynthetic microalgae drainage method adopts a sterile closed environment in the whole process, so that the obtained hydrogen-rich water is very clean and still grows in a sterile colony after being cultured on an LB culture medium for 24 hours; from the view of hydrogen production efficiency, although the photosynthetic microalgae needs more than 24 hours to obtain hydrogen-rich water, the photosynthetic microalgae still has continuous production capacity within the next 120 hours, and reaches linear speed within 48-72 hours, and more than 10L of hydrogen-rich water can be obtained from 2L of microalgae culture solution to 120 hours; the photosynthetic microalgae can produce 10L hydrogen gas while producing 10L hydrogen-rich water, and the method can simultaneously obtain high-purity hydrogen gas for treatment.
The invention utilizes microalgae to convert sunlight into hydrogen, feeds the hydrogen into sterile physiological saline in real time through a drainage method, dissolves the hydrogen into the physiological saline by utilizing the pressure of the hydrogen generated by the microalgae, discharges the physiological saline which is hydrogen-rich water, and collects hydrogen gas with extremely high purity. Compared with the traditional hydrogen-rich water preparation method, the method has the characteristics of cleanness, high efficiency and safety.
Drawings
Fig. 1 is a schematic view of an apparatus for producing hydrogen-rich water of the present invention.
Fig. 2 is a graph of the volume of hydrogen-rich water collected in a vacuum bag as detected over time in an example of the present invention.
Fig. 3 is a graph showing the results of measuring the hydrogen content of the hydrogen-rich water prepared by the three methods in the example of the present invention.
Fig. 4 is a graph showing the results of measuring the bacterial content of hydrogen-rich water produced by three methods according to the example of the present invention.
Detailed Description
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified.
The flasks used in the following examples were 2L scott glass flasks;
the container filled with sterile normal saline is a toughened glass bottle with the capacity of 2L; the vacuum sterile bag, the conduit and the valve are made of PVC materials.
Fig. 1 is a schematic view of an apparatus for producing hydrogen-rich water of the present invention.
The device comprises a microalgae photosynthetic hydrogen production system, a drainage gas collection system and a hydrogen-rich water collection system. The microalgae photosynthetic hydrogen production system consists of a 2L microalgae culture bottle, a group of LED lamps (total 8), a magnetic stirrer, two solar panels and two storage batteries; the water drainage and gas collection system is a sterile glass bottle filled with sterilized normal saline; the hydrogen rich water collection system consists of four vacuum sterile bags in series. The three systems are connected by a sterile catheter, and the catheter is provided with a controllable valve.
This device theory of operation and operation are very simple, solar panel converts solar energy into the electric energy and stores in the battery, the battery supplies power to LED lamp and magnetic stirrers, it continuously produces hydrogen to support little algae,
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