阅读说明：本技术 两栖类标本的制作方法 (Method for manufacturing amphibious specimen ) 是由 余国华 杜丽娜 武正军 于 2019-10-16 设计创作，主要内容包括：本发明公开了一种两栖类标本的制作方法,包括步骤1：浸水,将采集到活的两栖类动物用清水冲洗,洗去其身体上附着的杂质；步骤2：麻醉,将清洗干净的两栖类动物进行麻醉处理,使两栖类动物处于放松状态；步骤3：姿态摆放：根据两栖类动物生活时的姿态,对麻醉后的两栖类动物进行姿态摆放并固定,形成两栖类标本雏形；步骤4：浸泡,将步骤3形成的两栖类标本雏形放置到标本瓶中,并用浸泡液进行浸泡。本发明提供的制作方法利用麻醉使两栖类动物处于放松状态,并在两栖类动物处于放松状态时对两栖类动物进行姿态摆放并固定,解决了目前两栖类标本制作过程中由于两栖类动物处于挣扎状态而导致标本形态难看的问题。(The invention discloses a method for manufacturing an amphibious specimen, which comprises the following steps of 1: soaking, washing the collected living amphibians with clear water, and washing away impurities attached to the bodies of the amphibians; step 2: performing anesthesia, namely performing anesthesia treatment on the cleaned amphibian to enable the amphibian to be in a relaxed state; and step 3: posture arrangement: placing and fixing the postures of the anesthetized amphibians according to the postures of the amphibians in life to form an amphibian specimen prototype; and 4, step 4: and (4) soaking, namely placing the amphibious specimen prototype formed in the step (3) into a specimen bottle, and soaking with a soaking solution. The manufacturing method provided by the invention enables the amphibian to be in a relaxed state by anesthesia, and the amphibian is placed and fixed in posture when the amphibian is in the relaxed state, so that the problem that the specimen form is hard to see because the amphibian is struggled in the conventional process for manufacturing the amphibian specimen is solved.)

1. The manufacturing method of the amphibious specimen is characterized by comprising the following steps of:

step 1: soaking, washing the collected living amphibians with clear water, and washing away impurities attached to the bodies of the amphibians;

step 2: performing anesthesia, namely performing anesthesia treatment on the cleaned amphibian to enable the amphibian to be in a relaxed state;

and step 3: posture arrangement: placing and fixing the postures of the anesthetized amphibians according to the postures of the amphibians in life to form an amphibian specimen prototype;

and 4, step 4: and (4) soaking, namely placing the amphibious specimen prototype formed in the step (3) into a specimen bottle, and soaking with a soaking solution.

2. The method for preparing an amphibian specimen according to claim 1, wherein the anesthesia treatment in step 2 is to immerse the amphibian in alcohol with a concentration of 20-30% for 5-10 minutes.

3. The method for preparing an amphibian specimen according to claim 1, wherein the step 3 of pose setting of the amphibian is performed according to the following steps:

step 31: firstly, two hind limbs of the amphibian are recovered forwards, and two fore limbs are retracted backwards;

step 32: the fingers and toes on the appendages of the amphibian are fully opened;

step 33: slightly pressing the waist of the amphibian downwards to keep the body of the amphibian straight;

step 34: and finally, lifting the head of the amphibian upwards to keep the head flat.

4. The method for preparing an amphibian specimen according to claim 1, wherein the step of fixing the amphibian in step 3 comprises the following steps: and (4) lightly spraying the fixing liquid to all parts of the body of the well-placed amphibian.

5. The method for preparing an amphibian specimen according to claim 4, wherein the amphibian wetted with the fixative is covered with a protective film, and the fixative is poured onto the protective film.

6. A method for preparing an amphibious specimen according to claim 4 or 5, wherein the fixing solution is absolute ethyl alcohol with a concentration of 99%.

7. The method for preparing an amphibious specimen according to claim 1, wherein the soaking solution in step 4 is alcohol with a concentration of 70-75%.

Technical Field

The application belongs to the technical field of manufacturing processes of animal posture specimens, and particularly relates to a manufacturing method of an amphibious specimen.

Background

Amphibians are a kind of original, early landfilled warm-blooded animals with naked skin, numerous secretory glands and mixed blood circulation, and mainly comprise newt, anomala and urodele, newt such as fish mud eel, anomala such as frogs and toads, and urodele such as giant salamanders.

In order to preserve the animal, plant, mineral and other material for a long time and maintain the original appearance as much as possible, the animal, plant, mineral and other materials are processed to form specimens for exhibition, demonstration, education, identification, examination and other various researches.

At present, most of amphibious specimens are made by utilizing formaldehyde to fix the form or directly put into high-concentration alcohol soaking liquid, the former can damage the DNA of the specimen to influence the subsequent DNA extraction which may be needed, and the latter can lead the amphibious specimen to be in a struggling state, the form is difficult to see, and the subsequent specimen measurement is influenced due to dehydration, form deformation and other reasons.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a method for manufacturing an amphibious specimen, which can ensure the attractive posture of the amphibious specimen, improve the verisimilitude of the amphibious specimen and does not damage DNA substances of the specimen.

In order to achieve the purpose of the invention, the method for manufacturing the amphibious specimen comprises the following steps:

step 1: soaking, washing the collected living amphibians with clear water, and washing away impurities attached to the bodies of the amphibians;

step 2: performing anesthesia, namely performing anesthesia treatment on the cleaned amphibian to enable the amphibian to be in a relaxed state;

and step 3: posture arrangement: placing and fixing the postures of the anesthetized amphibians according to the postures of the amphibians in life to form an amphibian specimen prototype;

and 4, step 4: and (4) soaking, namely placing the amphibious specimen prototype formed in the step (3) into a specimen bottle, and soaking with a soaking solution.

Specifically, the anesthesia treatment in the step 2 is to soak the amphibian in alcohol with the concentration of 20-30% for 5-10 minutes.

Specifically, the step 3 of carrying out posture arrangement on the amphibian is carried out according to the following steps:

step 31: firstly, two hind limbs of the amphibian are recovered forwards, and two fore limbs are retracted backwards;

step 32: the fingers and toes on the appendages of the amphibian are fully opened;

step 33: slightly pressing the waist of the amphibian downwards to keep the body of the amphibian straight;

step 34: and finally, lifting the drooping head of the amphibian upwards to keep the head flat.

Specifically, the step of fixing the placed amphibian in the step 3 is as follows: and (4) lightly spraying the fixing liquid to all parts of the body of the well-placed amphibian.

Specifically, a protective film is covered on the amphibian wetted with the fixing liquid, and the fixing liquid is poured on the protective film. The volatilization speed of the fixing liquid sprayed on all parts of the body of the amphibian is reduced through the protective film, and the water loss speed of the specimen is reduced, so that the specimen is slightly dehydrated.

Specifically, the stationary liquid is absolute ethyl alcohol with the concentration of 99%.

Specifically, the soaking solution in the step 4 is alcohol with the concentration of 70-75 percent

The invention has the beneficial effects that: the manufacturing method provided by the invention has the advantages that the amphibian is in a relaxed state by anesthesia, and the posture of the amphibian is placed and fixed when the amphibian is in the relaxed state, so that the problem that the specimen form is hard to see because the amphibian is struggled in the conventional process for manufacturing the amphibian specimen is solved, the attractiveness of the posture of the amphibian specimen is ensured, the verisimilitude of the specimen is improved, and the damage to the DNA of the specimen is avoided.

Detailed Description

The technical solutions claimed in the present application will be described in detail with reference to embodiments, so that the implementation process of how to apply the technical means to solve the technical problems and achieve the technical effects of the present application can be fully understood and implemented.

The method for manufacturing the amphibious specimen provided by the invention is utilized to realize the manufacturing of the amphibious specimen, and specifically comprises the following steps:

step 1: soaking, washing the collected living amphibians with clear water, and washing away impurities attached to the bodies of the amphibians;

step 2: performing anesthesia, namely performing anesthesia treatment on the cleaned amphibian to enable the amphibian to be in a relaxed state;

and step 3: posture arrangement: according to the living posture of the living body of the amphibian, placing and fixing the anesthetized amphibian to form an amphibian specimen prototype;

and 4, step 4: and (3) soaking, namely placing the amphibious specimen prototype formed in the step (3) into a specimen bottle, and soaking with a soaking solution, wherein the soaking solution is 70-75% alcohol and is used for long-term storage.

During anesthesia treatment, 20-30% alcohol is used for anesthesia of the amphibians, and the anesthesia time is generally 5-10 minutes according to the size of the individual amphibians.

When the anesthetized amphibian is placed, the placing can be carried out according to the living posture of the live amphibian, and the placing can be specifically carried out according to the following steps:

step 31: firstly, two hind limbs of the amphibian are recovered forwards, and two fore limbs are retracted backwards;

step 32: the fingers and toes on the appendages of the amphibian are fully opened;

step 33: slightly pressing the waist of the amphibian downwards to keep the body of the amphibian straight;

step 34: and finally, lifting the drooping head of the amphibian upwards to keep the head flat.

Wherein, the front half body and the head of the amphibious vehicle are both slightly lifted upwards. The reason for slightly pressing the back of the body and slightly raising the head during the process of fixing the specimen is to keep the body and the head of the specimen in a straight state as much as possible.

The specific method for fixing the placed amphibian in the step 3 is that fixing liquid is lightly sprayed to all parts of the body of the placed amphibian specimen, more fixing liquid can be sprayed to the appendage and the joint of the amphibian, the fixing liquid is repeatedly sprayed for 5-8 times, the specimen is lightly clamped by tweezers after being fixed for 10-15 minutes, and if all parts of the amphibian do not deform due to movement, successful fixing is indicated, and the embryonic shape of the amphibian specimen is formed.

The fixing liquid can be any one, and absolute ethyl alcohol with the concentration of 99% is adopted as the fixing liquid, and when the fixing liquid is used, a 5 ml suction pipe is used for sucking the fixing liquid and then the fixing liquid is lightly drenched onto the placed amphibians.

In order to reduce the volatilization speed of the stationary liquid, a protective film is covered on the amphibian body sprayed with the stationary liquid, and the stationary liquid is sprayed on the protective film, so that the volatilization speed of the stationary liquid is reduced, and the volatilization speed of water of the amphibian is also reduced. The protective film can be any substance with air permeability, such as a paper towel or a cloth towel; the fixing liquid sprayed on the protective film can moisten the protective film.

Animal specimens made by directly putting amphibians into soaking liquid by using a traditional method have the defects of rigid limbs, large mouth, serious dehydration and unreality, and influence on the accuracy of specimen measurement data.

The posture of the animal specimen prepared by the method is beautiful, the animal specimen is closer to the living state, the dehydration is small, the DNA of the animal specimen is not damaged, and the extraction of molecular materials possibly needed in the future is not influenced.

The foregoing description shows and describes several preferred embodiments of the present application, but as aforementioned, it is to be understood that the present application is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of numerous other combinations, modifications, and variations within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the application, which is to be protected by the claims appended hereto.