阅读说明：本技术 一种含有Acynonapyr的杀螨组合物、制剂剂型及其应用 (Acaricidal composition containing Acynonapyr, preparation formulation and application thereof ) 是由 周大伟 郑敬敏 马强 刘欢 于 2019-10-11 设计创作，主要内容包括：本发明公开了一种含有Acynonapyr的杀螨组合物、制剂剂型及其应用,属于杀螨剂领域；所述杀螨组合物的有效成分包括Acynonapyr和苯丁锡；两者的质量比为1:8～8:1,优选为1:6～2:1,更优选为1:3～1:1；按质量百分比计,所述杀螨组合物的含量为1%～50%；本组合物可配制成农业上允许的剂型有悬浮剂、可分散油悬浮剂、可湿性粉剂、乳油。本发明组分合理,对农业害螨具有较好的防治效果,与现有单一制剂相比,具有显著的增效作用,能够降低农药使用量与成本,减少环境的污染,延长农药使用寿命。(The invention discloses an acaricidal composition containing Acynonapyr, a preparation formulation and application thereof, belonging to the field of acaricides; the effective components of the acaricidal composition comprise Acynonapyr and fenbutatin oxide; the mass ratio of the two is 1: 8-8: 1, preferably 1: 6-2: 1, and more preferably 1: 3-1: 1; the content of the acaricidal composition is 1-50% by mass; the composition can be prepared into agriculturally allowable dosage forms such as suspending agents, dispersible oil suspending agents, wettable powder and missible oil. The invention has reasonable components, has better control effect on agricultural pest mites, has obvious synergistic effect compared with the prior single preparation, can reduce the usage amount and cost of pesticides, reduce environmental pollution and prolong the service life of the pesticides.)

1. An acaricidal composition containing Acynonapyr is characterized in that effective active ingredients comprise Acynonapyr and fenbutatin oxide; the mass ratio of the active ingredients Acynonapyr to fenbutatin oxide is 1: 8-8: 1.

2. The Acynonapyr-containing acaricidal composition according to claim 1, wherein the mass ratio of the Acynonapyr to the fenbutatin oxide is 1: 6-2: 1, and preferably 1: 3-1: 1.

3. An acaricidal composition preparation containing Acynonapyr, which is characterized by comprising an acaricidal active ingredient and an auxiliary agent, wherein the acaricidal active ingredient is the acaricidal composition in claim 1 or 2, and the acaricidal composition is contained in an amount of 1-50% by mass.

4. The acaricidal composition formulation according to claim 3 containing Acynonapyr, wherein said composition is in the form of suspension, dispersible oil suspension, wettable powder, emulsifiable concentrate.

5. Use of the acaricidal composition comprising acyclonapyr according to claim 1 for controlling agricultural mites.

6. The use of claim 5, wherein the pest mites are one or more of Tetranychus viennensis, Tetranychus cinnabarinus, Panonychus ulmi and Tetranychus urticae.

Technical Field

The invention belongs to the field of acaricide compound pesticides, and relates to an acaricide composition containing Acynonapyr as effective active ingredients, a preparation formulation and application thereof.

Background

Acytonapyr, chemical name: (3-endo) -3- [ 2-propoxy-4- (trifluoromethyl) phenoxy ] -9- [ [5- (trifluoromethyl) -2-pyridinyl ] oxy ] -9-azabicyclo [3.3.1] nonane. The structural formula is shown as formula 1. Is an acaricide with cyclic amine skeleton developed by Nippon Caoda corporation, which acts on inhibitory glutamate receptors to interfere the neurotransmission of harmful mites, resulting in dyskinesia of the harmful mites and ultimately killing of the harmful mites. The agent can be used for preventing and treating vegetable, fruit tree and tea tree mites.

Formula 1

Fenbutatin oxide, english name: fenbutatin oxide, chemical name: bis [ tris (2-methyl-2-phenylpropyl) tin ] oxide. The structural formula is shown as formula 2. The acaricide has high acaricidal activity, mainly plays a role in contact killing, has strong killing power on young mites and adult mites and nymphs, can be used for crops such as fruit trees, tea trees, flowers and the like, and can be used for preventing and treating panonychus citri, citrus leaf mites, citrus rust mites, apple leaf mites, tea orange gall mites, tea short-fiber mites, chrysanthemum leaf mites, rose leaf mites and the like.

Formula 2

Agricultural harmful mites in China mainly comprise tetranychid mites, gall mites and tarsal mites, wherein the tetranychid mites are the largest group. The harmful characteristics of a plurality of harmful mites are very similar, the harmful mites are often gathered on the back of leaves, and adults and nymphs prick the leaves with mouthparts to suck juice, so that chlorophyll of plants is damaged, and photosynthesis is influenced. The damaged leaves often appear grey white spots or white patches, and some of the damaged leaves cause the proliferation of cells at the damaged parts, so that the leaves are raised in galls or gallballs, or the leaves are curled and shrunk, and the leaves die and fall off in severe cases, even plants die due to the falling of the leaves. In addition, many pest mites also transmit plant viral and fungal diseases. At present, a plurality of harmful mites have generated resistance to acaricides due to the long-term single use and even abuse of the acaricides.

Disclosure of Invention

The invention aims to provide the acaricidal composition which has reasonable components, remarkable synergistic effect, good acaricidal effect, low medication cost and safety to crops. Wherein the first active ingredient is Acynonapyr, and the second active ingredient is fenbutatin oxide.

The invention also aims to provide a preparation containing the acaricidal composition, which comprises a suspending agent, a dispersible oil suspending agent, wettable powder and missible oil.

Still another object of the present invention is to provide the use of the above composition for agricultural pest mites.

In order to achieve the purpose, the technical scheme of the invention is as follows:

an acaricidal composition containing Acynonapyr, which is characterized in that,

(1) a first active ingredient: acynonapyr;

(2) the second active ingredient: fenbutatin oxide

The weight ratio of the first active ingredient to the second active ingredient is 1: 8-8: 1, preferably 1: 6-2: 1, and more preferably 1: 3-1: 1.

The invention relates to an acaricidal composition containing Acynonapyr, a preparation formulation and an application, wherein the composition accounts for 1-50% of the total content of the preparation in percentage by mass.

The acaricidal composition containing Acynonapyr can be prepared into the dosage forms of suspending agents, dispersible oil suspending agents, wettable powder and missible oil according to the method known by the technical personnel in the technical field.

For the suspending agents, usable are: dispersing agent such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, nekal, dodecyl polyoxyethylene ether phosphate; wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, phenethylphenol polyoxyethylene ether phosphate ester, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenylethylphenol polyoxypropylene polyoxyethylene block polymer; thickening agent such as xanthan gum, polyvinyl alcohol, bentonite, magnesium aluminum silicate, carboxymethyl cellulose, carboxyethyl cellulose, and methyl cellulose; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate, potassium sorbate, isothiazolinone; defoaming agents such as silicone oil, silicone compound, C10-20 saturated fatty acid compound, C8-10 fatty alcohol, hexanol, butanol, octanol; antifreeze agent such as one or more of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, sorbitol, urea, and inorganic salt; the water is deionized water.

For dispersible oil suspensions, the adjuvants that can be used are: dispersing agent such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate (dispersant NNO), TERSPERSE 2020 (product of Huntsman corporation, U.S. alkyl naphthalene sulfonate); the emulsifier is selected from BY (polyoxyethylene castor oil) series emulsifier (BY-110, BY-125, BY-140), agricultural milk 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 2201, span-60 # (common name: sorbitan monostearate), Tween-60 # (common name: sorbitan monostearate polyoxyethylene ether), agricultural milk 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), and TERSPERSE 4894 (produced BY Henshimei corporation, USA); wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, TERSPERSE 2500 (available from henseme, usa); thickening agent such as one or more of white carbon black, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride; dispersing medium such as one or more of soybean oil, oleum Rapae, wheat oil, methyl oleate, diesel oil, engine oil, and mineral oil.

For wettable powders, the auxiliaries which can be used are: dispersing agents such as one or more of polycarboxylate, lignosulfonate, polyoxyethylene polyoxypropylene ether block copolymer, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate and alkylphenol polyoxyethylene ether sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate; the filler is one or more of ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite and pottery clay.

For emulsifiable concentrates, the auxiliaries which can be used are: emulsifiers such as calcium dodecylbenzene sulfonate (Nongru 500 #), Nongru 700#, Nongru 2201, span-60 #, emulsifier T-60 (common name: sorbitan monostearate polyoxyethylene ether), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Nongru 1601#, Nongru 600# and Nongru 400 #; solvents such as xylene, mineral spirits (S-150, S-180, S-200), toluene, biodiesel, methyl esterified vegetable oils, N-methyl pyrrolidone; co-solvents such as ethyl acetate, methanol, dimethylformamide, cyclohexanone, acetone, methyl ethyl ketone; stabilizers such as triphenyl phosphite, epichlorohydrin, acetic anhydride.

The application of the invention to agricultural pest mites includes the following experimental objects:

tetranychus viennensis, the scientific name of Latin is:Tetranychus viennensisbelonging to the order of the Mandarin, the family of Phyllanthus. Can be parasitized on various fruit trees such as pear, apple, peach, cherry, hawthorn, plum, etc. The main hazard modes are as follows: sucking the juice of the leaves and the young buds. After the leaves are seriously damaged, a plurality of small green spots appear firstly, then the small green spots are enlarged and connected into pieces, and when the leaves are seriously damaged, the whole leaves become yellow brown to fall off, so that the growth of fruit trees is seriously inhibited, even secondary flowering is caused, and the formation of flower buds in the current year and the yield in the next year are influenced.

Tetranychus cinnabarinus, the scientific name of latin is:Tetranychus cinnabarinus，belonging to the order Acarina, Phyllanthus. The hosts mainly comprise: eggplant, hot pepper, watermelon, beans, shallot and amaranth. The main hazard modes are as follows: the juice of the stem and leaf of the plant is absorbed, so that the water content of the affected part is reduced, the affected part is green and white, dense and pale speckles appear on the surface of the leaf, and the leaf curls and turns yellow. In severe cases, yellow leaves, scorched leaves, curled leaves, fallen leaves, death and the like occur to plants.

The Panonychus ulmi Karsch, Latin, is known by the chemical name:Panonychus ulmibelonging to the order of the Mandarin, the family of Phyllanthus. The main hosts comprise apples, pears, peaches, plums, apricots, hawthorns, Chinese hawthorn fruits, Chinese flowering crabapples, cherries, ornamental plants such as cherry blossom and roses, and leaves, young tips, flower buds and young fruits of the plants are damaged. Master and slaveThe method for harming the artificial skin comprises the following steps: when the juice of the leaves is absorbed by thorns, the leaves are faded and pale, the seriously damaged leaves are scorched and are like a burnt shape, the leaves fall in advance, and when the damage is serious, the newly germinated tender buds are withered, so that a large amount of flowers and fruits fall.

Tetranychus urticae Koch, Latin's nameTetranychus urticaeBelonging to the order Acarina, Tetranychidae. Can be parasitized on various fruit trees such as apple, peach, pear, apricot, plum and the like and weeds such as chenopodium album, amaranth and the like. The main hazard modes are as follows: parasitizing on the back of plant leaves to take food, piercing cells and sucking juice, wherein pale spots appear on the damaged leaves from two sides of a main vein close to a petiole, the leaves can become grey white and dark brown along with the aggravation of harm, the normal operation of photosynthesis is inhibited, and the serious leaves are scorched to fall off in advance, so that the tree vigor, the fruit quality and the fruit yield are seriously influenced.

The invention provides an acaricide composition, and the activity and the acaricidal effect of the acaricide composition are not simple superposition of the activities of all components, but have a certain synergistic effect, so that the dosage per unit area is obviously reduced, the dosage cost is reduced, the acaricide composition has good safety to non-target organisms, and meets the safety requirement of pesticide preparations.

Detailed Description

The synergistic effect of the compounding of Acynonapyr and fenbutatin oxide on agricultural pest mites is illustrated by indoor toxicity test.

The indoor toxicity determination method refers to part 12 of pesticide of NY/T1154.12-2008 pesticide indoor bioassay test criteria: tetranychus slide soaking method.

Preparation of test materials: the mites to be tested are selected from female adult mites collected in the field and bred indoors. Firstly, cutting a double-sided adhesive tape into 2-3 cm long, sticking the double-sided adhesive tape to one end of a glass slide, then selecting female adult mites which are bred in a room and have consistent physiological states, sticking the backs of the female adult mites to the double-sided adhesive, paying attention to no need of sticking mite feet, tentacles and mouthparts, placing each female adult mite with 30 heads into a container padded with wet sponge, covering the container with a cover, culturing the female adult mite with the double-sided adhesive at the temperature of 25 +/-1 ℃, performing microscopic examination after 2 hours, removing the female adult mite with death and injury individuals, and then supplementing 30 heads.

Medicament treatment: placing the glass slide in the prepared liquid medicine, slightly shaking for 5s, taking out, then sucking off the redundant liquid medicine by using absorbent paper, placing the glass slide in a white porcelain plate padded with wet sponge, and covering the white porcelain plate with a thin film with good light transmittance. The porcelain plate containing the treated test mites is kept and observed under the conditions that the temperature is 25 +/-1 ℃ and the light cycle is L: D = (16, 8) h.

The death of the test mites was checked 48h after the treatment and the total and dead mite numbers were recorded separately. Each treatment was repeated 4 times, and the clear water treatment was set as a blank control. The test data is statistically processed by DPS software to obtain correlation coefficient, virulence regression equation, lethal middle concentration, etc., and the cotoxicity coefficient is obtained by Sun cloud Pepper method.

The joint virulence of the mixed preparation is expressed by adopting a Sunweier cotoxicity coefficient method:

the combined action of the acaricidal composition on different mites is evaluated by the co-toxicity coefficient, the co-toxicity coefficient is more than 120 and is expressed as a synergistic action, the co-toxicity coefficient is between 80 and 120 and is expressed as an additive action, and the co-toxicity coefficient is less than 80 and is expressed as an antagonistic action. The toxicity test results of the Acynonapyr and fenbutatin oxide mixture on tetranychus cinnabarinus are shown in table 1; toxicity test results of Acynonapyr and fenbutatin oxide mixed to hawthorn leaf mites are shown in Table 2.

TABLE 1 toxicity test results of Acynonapyr and fenbutatin oxide mixture against Tetranychus cinnabarinus

From table 1, it can be seen: acynonapyr and fenbutatin oxide are mixed according to the weight ratio of 1: 8-8: 1, the composition shows synergistic or additive virulence to Tetranychus cinnabarinus. When the composition is compounded within the range of 1: 4-2: 1, the co-toxicity coefficients are all larger than 120, and the synergistic effect is shown; when the mixing proportion is 1:2, the co-toxicity coefficient is the largest, and the synergistic effect is the most obvious.

Table 2 toxicity test results of Acynonapyr and fenbutatin oxide mixture on tetranychus urticae

From table 2, it can be seen that the combination of acyclonapyr and fenbutatin oxide at a ratio of 1:8 to 8:1 exhibits synergistic or additive virulence effects on tetranychus viennensis. The mixing proportion is 1: 6-2: when 1, the co-toxicity coefficients are all larger than 120, and the synergistic effect is shown on tetranychus viennensis; when the mixing proportion is 1:3, the co-toxicity coefficient is the largest, and the synergistic effect is the most obvious.

The present invention will be further described with reference to the following examples.

Formulation example 1

50 g of Acynonapyr, 300 g of fenbutatin oxide, 50 g of polyoxyethylene polyoxypropylene ether block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀And when the suspension is smaller than 10 mu m, 35 percent of Acynonapyr tinoxide suspension is prepared.

Formulation example 2

50 g of Acynonapyr, 300 g of fenbutatin oxide, 40 g of nekal BX (sodium dibutylnaphthalenesulfonate), 40 g of sodium lignosulfonate, 25 g of sodium dodecyl sulfate and the talcum powder are weighed to be added to 1000 g. The raw materials are mixed, subjected to superfine airflow crushing and mixed to prepare 35% Acynonapyr fenbutatin wettable powder.

Formulation example 3

50 g of Acynonapyr, 200 g of fenbutatin oxide, 70 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium lignosulfonate, 20 g of carboxyethyl cellulose, 30 g of glycerol, 5 g of sodium benzoate, 15 g of octanol are weighed, and deionized water is added to 1000 g of parts by weight. Mixing the above raw materialsShearing at high speed for 30min, and sanding to obtain particle diameter D₉₀And when the suspension is smaller than 10 mu m, the 25 percent Acynonapyr fenbutatin oxide suspension is prepared.

Formulation example 4

50 g of Acynonapyr, 200 g of fenbutatin oxide, 30 g of T36 (polycarboxylate), 40 g of sodium alkyl naphthalene formaldehyde condensate sulfonate and 40 g of sodium dodecyl sulfate are weighed, and the amount of urea is increased to 1000 g. The raw materials are mixed, subjected to superfine airflow crushing and mixed to prepare 25% Acynonapyr fenbutatin wettable powder.

Formulation example 5

50 g of Acynonapyr, 150 g of fenbutatin oxide, 50 g of polyoxyethylene polyoxypropylene ether block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀And obtaining 20 percent Acynonapyr tinoxide suspending agent after the suspension is less than 10 mu m.

Formulation example 6

50 g of Acynonapyr, 150 g of fenbutatin oxide, 40 g of nekal BX (sodium dibutylnaphthalenesulfonate), 40 g of sodium lignosulfonate, 25 g of sodium dodecyl sulfate and the talcum powder are weighed to be added to 1000 g. The raw materials are mixed, subjected to superfine airflow crushing and mixed to prepare 20% Acynonapyr fenbutatin wettable powder.

Formulation example 7

50 g of Acynonapyr, 100 g of fenbutatin oxide, 70 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium lignosulfonate, 20 g of carboxyethyl cellulose, 30 g of glycerol, 5 g of sodium benzoate, 15 g of octanol are weighed, and deionized water is added to 1000 g of parts by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀And obtaining the 15 percent Acynonapyr fenbutatin oxide suspending agent after the particle size is less than 10 mu m.

Formulation example 8

50 g of Acynonapyr, 100 g of fenbutatin oxide, 30 g of T36 (polycarboxylate), 40 g of sodium alkyl naphthalene formaldehyde condensate sulfonate and 40 g of sodium dodecyl sulfate are weighed, and the amount of urea is increased to 1000 g. The raw materials are mixed, subjected to superfine airflow crushing and mixed to prepare the 15 percent Acynonapyr fenbutatin wettable powder.

Formulation example 9

50 g of Acynonapyr, 50 g of fenbutatin oxide, 50 g of polyoxyethylene polyoxypropylene block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀And obtaining the 10 percent Acynonapyr fenbutatin oxide suspending agent after the particle size is less than 10 mu m.

Formulation example 10

50 g of Acynonapyr, 50 g of fenbutatin oxide, 40 g of Lakai powder BX (sodium dibutylnaphthalenesulfonate), 40 g of sodium lignosulfonate, 25 g of sodium dodecyl sulfate and the talcum powder are weighed to be added to 1000 g. The raw materials are mixed, subjected to superfine airflow crushing and mixed to prepare the 10% Acynonapyr fenbutatin wettable powder.

The control effect of the compound preparation in the field is measured under the field condition in the following biological examples 1, 2 and 3. The test is carried out by referring to the 'pesticide field efficacy test criterion (I) that acaricide prevents and treats tetranychus ulmi'.

The test method comprises the following steps: all the districts are arranged randomly, the cultivation conditions of all the districts are consistent, 3 trees in each district are diluted by water and sprayed, each treatment is repeated for 4 times, a 10-time hand-held magnifier is used for sampling randomly to investigate leaves, and the pesticide is applied when 3-5 live mites exist in each visual field on average.

The investigation method comprises the following steps: each district investigates 2 trees, 5 damaged leaves in the same season are marked in the east, west, south, north and middle five directions of the trees, and the number of active mites on 50 leaves is investigated in each district. And directly observing the front and back surfaces of the leaf by using a handheld magnifier, and counting the number of live mites. If the damage is light, the number of investigation blades can be increased. The number of active mites was recorded 3 days, 7 days, 12 days, 20 days, and 25 days after the treatment.

PTThe mite mouth decline rate in the medicament treatment area;CKthe rate of oral mite decline in the blank control area is shown.

Biological example 1: and (3) field pesticide effect test for preventing and controlling panonychus ulmi.

In 5-month middle ten years in 2018, field tests for controlling panonychus ulmi by using preparation example 1 (35% of Acynonapyr/fenbutatin oxide suspending agent), preparation example 3 (25% of Acynonapyr/fenbutatin oxide suspending agent), preparation example 5 (20% of Acynonapyr/fenbutatin oxide suspending agent), preparation example 7 (15% of Acynonapyr/fenbutatin oxide suspending agent) and preparation example 9 (10% of Acynonapyr/fenbutatin oxide suspending agent) are carried out, and the synergistic effect after compounding is verified by comparing the effects of the control agents 20% of Acynonapyr suspending agent and 20% of fenbutatin oxide suspending agent. The test results are shown in Table 3.

Table 3 field pesticide effect test result of Acynonapyr and fenbutatin oxide compounded control of panonychus ulmi

From table 3, it can be seen: when the dosage of the effective components of the Acynonapyr and fenbutatin oxide compounded preparation is 100mg/kg, the control effect on panonychus ulmi 3, 7, 12, 20 and 25 days after the application is higher than the control effects of 100mg/kg of 20% of Acynonapyr suspending agent and 100mg/kg of 20% of fenbutatin oxide suspending agent, which shows that the compounding of the Acynonapyr and the fenbutatin oxide has obvious synergistic effect. Safety survey during test period shows that all the preparations are safe to apple growth, do not have the phenomenon of phytotoxicity and are harmless to natural enemies.

Biological example 3: and (3) field pesticide effect test for controlling tetranychus viennensis.

In 5-month middle ten years in 2018, field tests for controlling leaf mites of hawthorn are performed on formulation example 2 (35% acyclononapy-fenbutatin wettable powder), formulation example 4 (25% acyclononapyr-fenbutatin wettable powder), formulation example 6 (20% acyclononapyr-fenbutatin wettable powder), formulation example 8 (15% acyclononapyr-fenbutatin wettable powder) and formulation example 10 (10% acyclononapyr-fenbutatin wettable powder), and the synergistic effect after compounding is verified by comparing the effects of the control agents 20% acyclononapyr suspending agent and 20% fenbutatin suspending agent. The test results are shown in Table 4.

Table 4 field pesticide effect test results of Acynonapyr and fenbutatin oxide compounded control of tetranychus urticae

From table 4, when the dosage of the effective components of the compound preparation of acyclononapyr and fenbutatin oxide is 100mg/kg, the control effect on tetranychus urticae in 3, 7, 12, 20 and 25 days after the preparation is higher than the control effects of 100mg/kg of 20% acyclononapyr suspending agent and 100mg/kg of 20% fenbutatin oxide suspending agent, which indicates that the compound preparation of acyclononapyr and fenbutatin oxide has obvious synergistic effect. Safety survey during test period shows that all the preparations are safe to apple growth, do not have the phenomenon of phytotoxicity and are harmless to natural enemies.

Biological example 3: and (3) field pesticide effect test for preventing and treating tetranychus urticae.

In the early 5 th 2018, field tests for preventing and treating two-spotted spider mites are carried out on formulation example 1 (35% of Acynonapy suspension agent), formulation example 3 (25% of Acynonapy suspension agent), formulation example 5 (20% of Acynonapy suspension agent), formulation example 7 (15% of Acynonapy suspension agent) and formulation example 9 (10% of Acynonapy suspension agent) in the spring county of Suiyang city of Shaanxi province, and the synergistic effect after compounding is verified by comparing the effects of the control agents of 20% of Acynonapy suspension agent and 20% of fenbutatin suspension agent. The test results are shown in Table 5.

Table 5 results of field efficacy test of Acynonapyr and fenbutatin oxide for controlling tetranychus urticae

From table 5, when the dosage of the effective components of the compound preparation of acyclononapyr and fenbutatin oxide is 100mg/kg, the control effect on panonychus ulmi in 3, 7, 12, 20 and 25 days after the preparation is higher than the control effects of 20% of acyclononapyr suspending agent 100mg/kg and 20% of fenbutatin oxide suspending agent 100mg/kg, which indicates that the compound preparation of acyclononapyr and fenbutatin oxide has obvious synergistic effect. Safety survey during test period shows that all the preparations are safe to apple growth, do not have the phenomenon of phytotoxicity and are harmless to natural enemies.

In conclusion, the composition adopts a compounding scheme of two active ingredients, the activity and the acaricidal effect are not simple superposition of the activities of the components, and compared with the existing single preparation, the composition has obvious acaricidal effect, also has obvious synergistic effect, is low in prevention and treatment cost, is safe for crops, meets the safety requirement of pesticide preparations, and is an ideal medicament for preventing and treating pest mites.