阅读说明：本技术 一种植物提取物组合物及其在防晒、晒后修复、防光老化和美白化妆品中的应用 (Plant extract composition and application thereof in sunscreen, after-sun repair, photoaging prevention and whitening cosmetics ) 是由 叶希韵 周艳卿 尹尉翰 刘明耀 于 2018-07-02 设计创作，主要内容包括：本发明公开了一种天然植物迷迭香、金银花及川芎提取物组合物在防晒、晒后修复、抗炎、防光老化和美白化妆品中的应用,属于中药及化妆品领域。本发明将迷迭香、金银花及川芎提取物应用于化妆品,验证了其中两种或三种组分的组合能有效抵抗紫外线对皮肤造成的损伤,并对紫外线损伤后的皮肤细胞修复具有明显效果,本发明首次发现所述组合物中的组成成分的组合使用能明显提高防晒功效,晒后修复功效,具有抗炎、防光老化和美白的作用,能保持皮肤湿润,增加皮肤晒后水分含量和皮肤弹性的作用,使用安全、稳定。本发明具有防晒、晒后修复、抗炎、防光老化和美白功效的植物提取物组合可作为有效活性成分制成膏霜、喷雾剂、粉剂等多种剂型,用于化妆品。(The invention discloses an application of a natural plant rosemary, honeysuckle and ligusticum wallichii extract composition in cosmetics for sun protection, after-sun repair, inflammation resistance, photoaging resistance and whitening, and belongs to the field of traditional Chinese medicines and cosmetics. The invention applies rosemary, honeysuckle and ligusticum wallichii extract to cosmetics, verifies that the combination of two or three components can effectively resist the damage of ultraviolet rays to the skin, and has obvious effect on repairing skin cells after the ultraviolet rays are damaged. The plant extract composition with the sun-screening, after-sun repairing, anti-inflammatory, anti-photoaging and whitening effects can be used as an effective active ingredient to be prepared into various formulations such as cream, spray, powder and the like, and is used for cosmetics.)

1. A plant extract composition for sun protection, after-sun repair, anti-inflammation, anti-photoaging, whitening, moisturizing or anti-aging is characterized by comprising any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract as active ingredients, wherein the mass part ratio of the two active ingredients is (1-9): (1-9); or the plant extract composition contains rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are mixed according to the mass part ratio of (1-9): (1-9): (1-9).

2. The plant extract composition as claimed in claim 1, wherein the ratio of any two active ingredients of rosemary extract, honeysuckle extract or chuanxiong rhizome extract in parts by weight is (4-6): (4-6).

3. The plant extract composition as claimed in claim 1, wherein the ratio of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract in parts by weight is 1: 1: 1.

4. a cosmetic composition comprising the plant extract composition according to any one of claims 1 to 3, wherein the active ingredient of any one of the extracts in the plant extract composition is 0.05% to 3% by weight of the cosmetic base, the balance being the cosmetic base.

5. The cosmetic composition of claim 4, wherein the active ingredient of any one of the extracts is 1% by weight of the cosmetic base.

6. Use of a plant extract composition according to any one of claims 1 to 3 and/or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a product for combating damage to the skin by ultraviolet light and for repairing or preventing photoaging after damage.

7. Use of a plant extract composition according to any one of claims 1 to 3 and/or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a cosmetic product having sun protection, after-sun repair, anti-inflammatory, anti-photoaging, whitening, moisturizing or anti-ageing efficacy.

8. Use of a plant extract composition according to any one of claims 1 to 3 and/or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a product intended to prevent the deposition of melanin in the skin, or to reduce the production of melanin in skin cells, or to degrade already deposited melanin.

9. Use of a plant extract composition according to any one of claims 1 to 3 and/or a cosmetic composition according to any one of claims 4 to 5 for the preparation of an anti-inflammatory product.

10. The use of claim 9, wherein the anti-inflammatory is preventing, reducing, or eliminating an inflammatory response in the skin.

11. Use of a plant extract composition according to any one of claims 1 to 3 and/or a cosmetic composition according to any one of claims 4 to 5 for the preparation of a sunscreen, a photoaging or a depigmenting cosmetic.

Technical Field

The invention belongs to the field of traditional Chinese medicines and cosmetics, and relates to a natural plant extract composition containing rosemary extract, honeysuckle extract and/or ligusticum wallichii composition and application thereof in sunscreen, after-sun repair, anti-inflammation, photoaging prevention and whitening cosmetics.

Background

In recent years, the increase of natural environmental pollution and atmospheric destruction has led to the increase of ultraviolet rays irradiated to the ground, and the number of patients with skin aging, solar dermatitis and even skin cancer has significantly increased, so that the search for safe, effective and stable cosmetic sunscreens and after-sun repair agents has been the focus of much research and development.

Among the three types of ultraviolet rays having different wavelength ranges, two portions of ultraviolet rays can be irradiated to the ground, i.e., the long-wavelength ultraviolet ray UVA having a wavelength of 320-. UVA has extremely strong penetrating power, and can reach the deep part of the dermis layer through the surface layer of the skin to cause premature aging of the skin; UVB mainly acts on the cell surface to cause skin tanning, and can cause erythema generation and peeling for a long time, even damage cell DNA and cause skin cancer. Naturally, UVA and UVB have a synergistic effect on skin damage, and UVA can significantly aggravate acute damage caused by UVB and increase carcinogenic potential, so daily protection against ultraviolet rays is very important.

The number of sunscreen cosmetics on the market is large, and the sunscreen capacity is mainly determined by sunscreen agents. Titanium dioxide, zinc oxide and other physical sun-screening agents can effectively shield the skin from being damaged by ultraviolet rays, but when the using amount is too large or the using time is long, the air permeability is poor, and pores are easy to block; chemical sunscreens such as benzophenone derivatives are also widely used, but they are likely to cause skin allergy, photolysis, and the like, and therefore, when they are used for a long period of time, the intermediate products generated are likely to cause damage to the skin.

Nowadays, the improvement of living standard leads people to continuously improve the requirements of cosmetics, the situation of skin care of the public is also more and more rational, the safety and effectiveness of the added components are more and more emphasized, and the cosmetics which are natural, stable and remarkable in sunscreen effect are favored. Research for many years shows that some traditional Chinese medicines can play an effective role in preventing and treating skin photodamage and photoaging, and multiple researches show that skin aging is closely related to improper sun protection, so that daily sun protection is very important, and the traditional Chinese medicines can remove oxygen free radicals, repair collagen fibers, improve microcirculation and the like, and the rhizoma kaempferiae extract, the curcuma longa rhizome extract, the aloe extract and the like have good sun protection effects. The sunscreen component extracted from the plant has stable drug property and lasting drug efficacy, has no stimulation to skin, is safe and effective, has wide development prospect, and is one of the market development directions of cosmetics.

Disclosure of Invention

The invention aims to provide a safe and effective natural plant extract composition which has the effects of sun-screening, after-sun repairing, anti-inflammation, anti-photoaging and whitening and moisturizing effects on skin cells.

The invention provides application of rosemary extract, honeysuckle extract, ligusticum wallichii extract and a composition thereof in sunscreen, after-sun repair, anti-inflammation, anti-photoaging, whitening, moisturizing or anti-aging of cosmetics.

The invention provides a plant extract composition with sun-screening, after-sun repairing and whitening, anti-inflammatory, anti-photoaging, moisturizing or anti-aging effects, wherein any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract are used as active ingredients of the plant extract composition, and the mass part ratio of the two active ingredients is (1-9): (1-9); or the plant extract composition contains rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are mixed according to the mass part ratio of (1-9): (1-9): (1-9).

In the invention, any two of rosemary extract, honeysuckle extract or ligusticum wallichii extract are taken as active ingredients of the plant extract composition, and the plant extract composition has a remarkable synergistic enhancement effect between any two of the rosemary extract, the honeysuckle extract or the ligusticum wallichii extract within the mass ratio range. From fig. 2 and fig. 5, it can be seen that the effect of the combination of rosemary extract and honeysuckle extract is better than that of the combination of rosemary extract and honeysuckle extract when the combination is used alone, and the combination has good anti-ultraviolet injury effect under different concentration ratios; similarly, as shown in fig. 3 and fig. 6, the effect of the combination of rosemary extract and chuanxiong rhizome extract is better than that of the combination of rosemary extract and chuanxiong rhizome extract when the combination is used alone, and the combination has good anti-ultraviolet injury effect under different concentration ratios; as shown in FIG. 4 and FIG. 7, the effect of the combination of the honeysuckle extract and the chuanxiong rhizome extract is better than that of the combination of the honeysuckle extract and the chuanxiong rhizome extract when the combination is used alone, and the honeysuckle extract and the chuanxiong rhizome extract have good ultraviolet injury resistance under different concentration ratios. Preferably, the mass part ratio of any two active ingredients is (4-6): (4-6); more preferably, the mass part ratio of any two active ingredients is 1: 1.

in the invention, the plant extract composition takes rosemary extract, honeysuckle extract and ligusticum wallichii extract as active ingredients, and preferably, the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract are in a mass part ratio of (4-6): (4-6): (4-6); more preferably, the mass part ratio of the rosemary extract to the honeysuckle extract to the ligusticum wallichii extract is 1: 1: 1. within the mass ratio range, the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract have a remarkable synergistic enhancement effect, namely as shown in fig. 8 and 9, compared with a single extract, the composition of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract has better protection and repair effects on ultraviolet irradiation.

The invention also provides a cosmetic composition containing the extract composition. Wherein the active component of each extract (rosemary extract, honeysuckle extract or ligusticum wallichii extract) accounts for 0.05-3% of the mass percentage of the cosmetic matrix, and the balance is the cosmetic matrix.

Preferably, the active ingredients of the composition (rosemary extract, honeysuckle extract or ligusticum wallichii extract) account for 3% of the mass of the cosmetic matrix, and the balance is the cosmetic matrix.

In the present invention, the cosmetic base may be, but is not limited to, a base mixture (weight percentage) consisting of:

6% glycerol, 5% mineral oil, 4% isopropyl palmitate, 3.5% caprylic/capric triglyceride, 2% steareth-2, 2% steareth-21, 1.5% cetearyl alcohol, 1% polyacrylate-13/polyisobutylene/polysorbate-20, 0.8% glyceryl stearate, 0.2% allantoin, 0.2% methylparaben, 0.15% bis (hydroxymethyl) imidazolidinyl urea, 0.1% propylparaben, 0.05% disodium EDTA, and the balance water. Or the like, or, alternatively,

6% cetearyl ethyl hexanoate, 5% glycerol, 4% ethyl oleate, ethyl stearate, shea butter unsaponifiable matter, ethyl linoleate, 3% caprylic capric triglyceride, 3% methyl propylene glycol, 3% arachidyl alcohol, behenyl alcohol, arachidyl alcohol glucoside, water, glucose, 2.5% cetearyl alcohol, 2% polydimethylsiloxane, 1% C14-22 alcohol, C12-20 alkyl glucoside, water, glucose, 0.6% phenoxyethanol, 0.5% polyacrylate crosspolymer-6, water, tert-butanol, 0.2% ethylhexyl glycerol, 0.2% hexylene glycol/caprylyl glycol, 0.2% gum arabic, xanthan gum, and the balance water.

The cream prepared by the method is uniform in coating, fine in hand feeling, easy to absorb, light in natural herbal fragrance, and fresh and mild.

The invention also provides application of the plant extract composition or the cosmetic composition in preparing products for resisting damage of ultraviolet rays to skin and repairing damaged skin.

The invention also provides application of the plant extract composition or the cosmetic composition in preparing cosmetics with sun-screening, after-sun repairing, anti-inflammatory, anti-photoaging, whitening, moisturizing or anti-aging effects.

The invention also provides application of the plant extract composition or the cosmetic composition in preparing sunscreen cosmetics or freckle-removing cosmetics.

In the invention, the cosmetic can be various cosmetics such as cream, emulsion, facial mask, toner, skin lotion, ointment, oil solution, gel, aerosol, solution, or film coating agent.

The invention verifies the effects of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract on the aspects of ultraviolet resistance, sun protection, after-sun repair, inflammation resistance, light aging resistance, whitening and moisture retention of skin, and verifies the synergistic effect of the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract on the aspects of sun protection, after-sun repair, inflammation resistance, light aging resistance and whitening for the first time through in vitro and in vivo experiments. The experimental result shows that the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can effectively enhance the ultraviolet ray resistance of the skin, have obvious repairing effect on cells damaged by ultraviolet rays, and can also obviously reduce the inflammatory reaction of the skin and the formation of melanin. The raw materials of rosemary extract, honeysuckle extract and ligusticum wallichii extract are natural and green, are non-toxic and non-irritant, and have the advantages of simple process, low production cost, mild effect on skin, safety and effectiveness.

In the present invention, the whitening refers to preventing the deposition of melanin in the skin, or reducing the production of melanin in skin cells, or degrading already deposited melanin.

In the invention, the anti-inflammation refers to the application of the product for preventing, reducing and eliminating skin inflammatory reaction. Wherein the inflammation refers to skin inflammation reaction caused by ultraviolet irradiation, and can reduce the production of various intracellular inflammatory factors.

In the present invention, the moisturizing means maintaining the skin moist, keeping the skin moist and elastic, or making the dry skin moist and elastic.

The invention has the beneficial effects that the invention provides the application of the composition containing the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract in the preparation of cosmetics for preventing sun, repairing after sun, resisting inflammation, preventing photoaging and whitening. The invention applies rosemary extract, honeysuckle extract and ligusticum wallichii extract to cosmetics, firstly provides that the composition of any two active components has the effects of sun screening, after-sun repair, inflammation resistance, photoaging resistance, whitening and the like, and further provides that the composition formed by combining the rosemary extract, the honeysuckle extract and the ligusticum wallichii extract can greatly improve the sun screening effect and the after-sun cell repair effect, is safe and stable, keeps skin moist, whitens the skin, prevents photoaging and resists skin aging. The invention can be used as an effective active ingredient to be prepared into various formulations such as cream, spray, powder and the like, and is widely applied to cosmetics, skin care products and the like.

Drawings

FIG. 1 is a graph showing the effect of rosemary extract, honeysuckle extract and chuanxiong rhizome extract on cell activity, and FIGS. 1-1 show the effect of rosemary extract at different concentrations on cell activity; FIGS. 1-2 the effect of different concentrations of honeysuckle extract on cell viability; FIGS. 1-3 the effect of different concentrations of Chuan Xiong extract on cell activity; p <0.05 compared to control group.

FIG. 2 is a graph comparing the protective effect of the composition on HaCaT cells by UV irradiation with rosemary extract and honeysuckle extract, p, compared with the UV control<0.05，**p<0.01，***p<0.001; compared with the composition group,^##p<0.01，^###p<0.001。

FIG. 3 is a graph comparing the protective effect of UV irradiation HaCaT cells of the composition with rosemary extract and Chuan Xiong extract, and UV control<0.01，***p<0.001; compared with the composition group,^#p<0.05，^##p<0.01，^###p<0.001。

FIG. 4 is a graph comparing the protective effect of the composition on HaCaT cells by UV irradiation with flos Lonicerae extract and rhizoma Ligustici Chuanxiong extract, and p is compared with ultraviolet control group<0.05，**p<0.01，***p<0.001; compared with the composition group,^#p<0.05，^###p<0.001。

fig. 5 is a graph showing the results of the uv protection experiment with rosemary extract and honeysuckle extract at different concentration ratios, compared to the uv control group, p <0.01 and p < 0.001.

Fig. 6 is a graph showing the results of the uv protection experiment with rosemary extract and chuanxiong rhizome extract at two different concentration ratios, p <0.01 and p <0.001, compared with the uv control group.

Fig. 7 is a graph showing the results of the protection experiment of uv light by using two different concentration ratios of the honeysuckle extract and the chuanxiong rhizome extract, wherein p is less than 0.01 and p is less than 0.001 compared with the uv control group.

FIG. 8 is a graph comparing the protective effect of the composition on HaCaT cells exposed to UV irradiation with Rosmarinus officinalis extract, Lonicera Japonica flos extract, and rhizoma Ligustici Chuanxiong extract, and comparing with UV control group<0.01，***p<0.001, compared to the composition group,^#p<0.05，^##p<0.01，^###P<0.001。

FIG. 9 is a graph comparing the effect of the composition on repairing HaCaT cell damage by UV irradiation with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and comparing with the UV control,^*P<0.05，**p<0.01，***p<0.001, compared to the composition group,^##p<0.01，^###p<0.001。

FIG. 10 is a graph comparing the anti-inflammatory effect of the composition on UV-irradiated HaCaT cells with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and FIG. 10-1 is a graph comparing the effect of the composition on UV-irradiated HaCaT cells IL-1 β with rosemary extract, honeysuckle extract, and Chuan Xiong extract, and compared with the UV control group<0.01，***p<0.001, compared to the composition group,^###p<0.001; FIG. 10-2 is a graph comparing the effect of the composition on UV irradiation of HaCaT cell IL-6 with rosemary extract, honeysuckle extract, and chuanxiong rhizome extract, and on UV irradiation of HaCaT cell IL-6 with UV control<0.05，**p<0.01，***p<0.001, compared to the composition group,^#p<0.05，^##p<0.01。

FIG. 11 is a graph comparing the inhibitory effect of the composition on melanin content in B16F10 cells, and rosemary extract, honeysuckle extract, and chuanxiong rhizome extract, with control group<0.01，***p<0.001, compared to the composition group,^#p<0.05，^##p<0.01。

Detailed Description

The present invention will be described in further detail with reference to the following specific examples and the accompanying drawings. The procedures, conditions, experimental methods and the like for carrying out the present invention are general knowledge and common general knowledge in the art, except for those specifically mentioned below, and the present invention is not particularly limited.

The rosemary extract is a cosmetic raw material and can be purchased from the market. Specific said rosemary extracts may include, but are not limited to, rosemary flower/leaf/stem extracts, rosemary flower extracts, rosemary leaf powders, rosemary leaf waters, rosemary leaf extracts, and rosemary leaf oils; preferably, it is rosemary leaf powder, rosemary flower/leaf/stem extract, rosemary flower extract, rosemary extract or rosemary leaf extract.

The honeysuckle extract (also called honeysuckle extract) is a cosmetic raw material and can be purchased from the market. Specific honeysuckle extracts may include, but are not limited to, honeysuckle flower extract, honeysuckle leaf extract, and honeysuckle stem extract.

The ligusticum wallichii extract is a cosmetic raw material and can be purchased from the market. The cnidium officinale Makino extract may include, but is not limited to, cnidium officinale Makino root powder, cnidium officinale Makino root extract, cnidium officinale Makino extract, and cnidium officinale Makino oil.

The composition can be prepared into various dosage forms such as cream, spray, powder and the like, and can be used as an active ingredient for cosmetics. The obtained cosmetic can be in the form of cream, emulsion, facial mask, astringent, skin caring lotion, ointment, oil, gel, aerosol, solution, or plastics.