阅读说明：本技术 一种分离式离心孔板、离心孔板组件及其自分离方法 (Separating centrifugal orifice plate, centrifugal orifice plate assembly and self-separating method thereof ) 是由 朱常兴 郑成 钟莲 于 2019-10-16 设计创作，主要内容包括：本发明实施例公开了一种分离式离心孔板、离心孔板组件及其自分离方法,分离式离心孔板包括可分离连接的底板和盖板,以及用于放置组合采样管的离心孔道；离心孔板组件包括分离式离心孔板和设置于其下方的支撑块；自分离方法包括通过支撑块与定位孔的配合来结合立柱实现离心后的分离。通过上述设置和操作,满足了高通量操作的需求,并且多个组合采样管的独立设置也避免了二次污染的问题；进一步地,在操作后结合支撑块的设置,有效实现自分离,大大提高了操作效率。(The embodiment of the invention discloses a separating centrifugal orifice plate, a centrifugal orifice plate assembly and a self-separating method thereof, wherein the separating centrifugal orifice plate comprises a base plate and a cover plate which are detachably connected, and a centrifugal pore channel for placing a combined sampling pipe; the centrifugal orifice plate component comprises a separated centrifugal orifice plate and a supporting block arranged below the separated centrifugal orifice plate; the self-separation method comprises the step of combining the upright column through the matching of the supporting block and the positioning hole to realize separation after centrifugation. Through the arrangement and the operation, the requirement of high-flux operation is met, and the problem of secondary pollution is avoided due to the independent arrangement of the multiple combined sampling pipes; furthermore, the self-separation is effectively realized by combining the arrangement of the supporting block after the operation, and the operation efficiency is greatly improved.)

1. The separating centrifugal orifice plate is characterized by comprising a bottom plate (1) and a cover plate (2) arranged above the bottom plate (1), wherein the bottom plate (1) and the cover plate (2) are detachably connected, and a plurality of centrifugal orifices (4) for placing combined sampling pipes (3) are formed between the bottom plate (1) and the cover plate (2);

the bottom plate (1) is downwards sunken and penetrated along the vertical direction to form a plurality of positioning holes (12), and the lower surface of the cover plate (2) extends downwards to form upright posts (22) inserted into the positioning holes (12).

2. A split centrifugal well plate according to claim 1, wherein the positioning hole (12) is formed as a cylindrical hole penetrating through the bottom plate (1), and the side surface of the cylindrical hole extends in the horizontal direction to form at least one spacing groove (121);

the upright post (22) comprises a fastening part (221) and an extension part (222), wherein the fastening part (221) and the extension part (222) are coaxially arranged from top to bottom and are formed into a cylinder, the fastening part (221) is tightly attached to the cylindrical hole, and the diameter of the extension part (222) is smaller than that of the fastening part (221).

3. The separating centrifugal orifice plate according to claim 1, characterized in that a plurality of insertion holes (11) are formed in the bottom plate (1) in a penetrating manner along a vertical direction, a plurality of through holes (21) corresponding to the insertion holes (11) in a one-to-one manner penetrate through the cover plate (2), and the insertion holes (11) and the through holes (21) are matched to form a centrifugal orifice (4).

4. A split centrifugal well plate according to claim 3, wherein the upper surface of the cover plate (2) extends upwards from the outer peripheral surface of the through hole (21) to form an annular multi-claw structure (23), and the multi-claw structure (23) is used for embedding the tube cover (33) of the combined sampling tube (3).

5. A split centrifugal well plate according to claim 1, characterized in that the inner side wall of the receptacle (11) is formed with an annular projection (111) in the circumferential direction, and the annular projection (111) is formed with a plurality of openings (112) in the vertical direction, and the outer surface of the combined sampling tube (3) is formed with an annular projection (31) in the circumferential direction for fitting under the annular projection (111).

6. A split centrifugal well plate according to claim 1, wherein the side wall of the centrifugal hole (4) is further extended outward to form a guide groove, and the outer side wall of the combined sampling tube (3) is extended outward to form a guide post (32) matching with the guide groove;

a plurality of protruding reinforcing ribs (13) are formed on the outer side surface of the bottom plate (1) along the vertical direction.

7. A centrifugal orifice plate assembly, comprising a separate centrifugal orifice plate according to any one of claims 1 to 6 and a support block (5) arranged below the separate centrifugal orifice plate; wherein the content of the first and second substances,

the supporting block (5) comprises a block body (51) and ejector rods (52) arranged on the upper surface of the block body (51), and the ejector rods (52) correspond to the positioning holes (12) one by one and are inserted into the positioning holes (12);

the sum of the height of the upright post (22) and the height of the ejector rod (52) is greater than the height of the positioning hole (12).

8. Centrifugal orifice plate assembly according to claim 7, in which the upper surface of the support block (5) is also formed with a plurality of locating blocks (53) in circumferential direction, which engage with the outer peripheral surface of the bottom plate (1).

9. A self-separation method of a centrifugal orifice plate, characterized in that the centrifugal orifice plate assembly of claim 7 is used, the self-separation method comprising:

s10, combining the separating centrifugal pore plates and placing the combined separating centrifugal pore plates in a centrifuge for centrifugation;

s20, placing the centrifuged separation type centrifugal orifice plate on a supporting block (5), and enabling the push rods (52) to be arranged below the positioning holes (12) in a one-to-one correspondence manner;

s30, ejecting the upright post (22) through the ejector rod (52), and separating the bottom plate (1) and the top plate (2).

10. A self-separation method of a centrifugal orifice plate, characterized in that the centrifugal orifice plate assembly of claim 7 is used, the self-separation method comprising:

s100, placing the supporting block (5) in a centrifuge;

s200, the separated centrifugal hole plates are correspondingly placed on the supporting blocks (5), and the ejector rods (52) are correspondingly arranged below the positioning holes (12) one by one, so that loose matching of the bottom plate (1) and the cover plate (2) is realized;

s300, after centrifugation, the bottom plate (1) is separated from the cover plate (2).

Technical Field

The embodiment of the invention relates to the field of microporous plates, in particular to a separating centrifugal orifice plate, a centrifugal orifice plate assembly and a self-separating method thereof.

Background

Separation and concentration of samples is a basic experimental procedure in biology, and laboratory personnel usually perform manual operations in a centrifuge by using centrifuge tubes or well plates with covers or screw caps.

With the continuous progress of science and technology, the current instruments and equipment in the fields of biology and medicine gradually develop towards high flux and automation, and when samples are very rich and high flux automation operation is required, a common centrifuge tube needs to be subjected to single tube operation, so that the efficiency is not high; the common centrifugal pore plate usually needs to be pre-treated and then transferred to the field for operation, so that secondary transfer of the sample is inevitably generated, and the possibility of sample pollution is increased.

In the field of high-throughput screening, for example, in high-throughput forensic DNA identification applications, high-throughput and pollution-free automated centrifugation is required, and it is ensured that samples are directly applied to high-throughput well plates without secondary transfer as much as possible.

Disclosure of Invention

Therefore, embodiments of the present invention provide a separation-type well plate, a centrifugal well plate assembly and a self-separation method thereof, so as to meet the requirement of effectively realizing high-throughput operation of collected samples on the premise of avoiding pollution caused by secondary transfer.

In order to achieve the above object, an embodiment of the present invention provides the following:

in one aspect of the embodiments of the present invention, there is provided a separating centrifugal orifice plate, including a bottom plate and a cover plate disposed above the bottom plate, the bottom plate and the cover plate being detachably connected, a plurality of centrifugal orifices for accommodating combined sampling tubes being formed between the bottom plate and the cover plate;

the bottom plate is downwards sunken and penetrated along the vertical direction to form a plurality of positioning holes, and the lower surface of the cover plate downwards extends to form an upright post inserted into the positioning holes.

As a preferable scheme of the present invention, the positioning hole penetrates through the bottom plate to form a cylindrical hole, and a side surface of the cylindrical hole extends in a horizontal direction to form at least one spacing groove;

the upright column comprises a fastening part and an extension part which are coaxially arranged from top to bottom and form a cylinder, the fastening part is tightly attached to the cylindrical hole, and the diameter of the extension part is smaller than that of the fastening part.

As a preferable mode of the present invention, the bottom plate is formed with a plurality of insertion holes penetrating in a vertical direction, the cover plate is formed with a plurality of through holes penetrating in one-to-one correspondence to the insertion holes, and the insertion holes and the through holes are formed in a matching manner to form centrifugal ducts.

In a preferred embodiment of the present invention, an annular multi-claw structure is formed on the upper surface of the cover plate extending upward from the outer circumferential surface of the through hole, and the multi-claw structure is used for engaging with the tube cap of the combined sampling tube.

As a preferable mode of the present invention, an annular protrusion is formed on an inner side wall of the insertion hole in a circumferential direction, the annular protrusion is formed with a plurality of openings in a vertical direction, and an annular protrusion for fitting under the annular protrusion is formed on an outer surface of the combined sampling tube in the circumferential direction.

As a preferable scheme of the invention, the side wall of the centrifugal hole further extends outwards to form a guide groove, and the outer side wall of the combined sampling tube extends outwards to form a guide post matched with the guide groove;

and a plurality of protruding reinforcing ribs are formed on the outer side surface of the bottom plate along the vertical direction.

In another aspect of an embodiment of the present invention, there is provided a centrifugal orifice plate assembly, including the separating centrifugal orifice plate as described above and a supporting block disposed below the separating centrifugal orifice plate; wherein the content of the first and second substances,

the supporting block comprises a block body and ejector rods arranged on the upper surface of the block body, and the ejector rods correspond to the positioning holes one by one and are inserted into the positioning holes;

the sum of the height of the upright post and the height of the ejector rod is greater than the height of the positioning hole.

In a preferred embodiment of the present invention, the upper surface of the supporting block further has a plurality of positioning blocks formed along a circumferential direction to be engaged with the outer circumferential surface of the base plate.

In another aspect of an embodiment of the present invention, there is provided a self-separation method of a centrifugal orifice plate, using the centrifugal orifice plate assembly described above, the self-separation method including:

s10, combining the separating centrifugal pore plates and placing the combined separating centrifugal pore plates in a centrifuge for centrifugation;

s20, placing the centrifuged separation type centrifugal orifice plate on a supporting block, and enabling the ejector rods to be arranged below the positioning holes in a one-to-one correspondence manner;

and S30, ejecting the upright post through the ejector rod, and separating the bottom plate from the top plate.

In another aspect of an embodiment of the present invention, there is provided a self-separation method of a centrifugal orifice plate, using the centrifugal orifice plate assembly described above, the self-separation method including:

s100, placing the supporting block in a centrifuge;

s200, the separated centrifugal hole plates are correspondingly placed on the supporting blocks, and the ejector rods are correspondingly arranged below the positioning holes one by one, so that loose matching of the bottom plate and the cover plate is realized;

and S300, after centrifugation, separating the bottom plate from the cover plate.

The embodiment of the invention has the following advantages:

1. according to the embodiment of the invention, the combined sampling pipe is directly placed in the bottom plate after sampling, and then the cover plate is covered, so that the mode of single sample centralized storage effectively avoids secondary transfer of samples; meanwhile, a plurality of groups of combined sampling tubes are placed in a centralized manner, so that the requirement of later-stage high-flux automatic operation is met; furthermore, the positioning holes are formed in the bottom plate, the upright posts matched with each other are arranged on the cover plate, stability is improved, and the application range of later-stage operation is better widened.

2. Further establish the supporting shoe through adding in the below of above-mentioned disconnect-type centrifugal orifice plate to set up bellied ejector pin on the supporting shoe and the mode that the locating hole corresponds, can pass through the stand in the ejector pin ejection locating hole, and realize the self-separation effect, improved the demand to high flux operation better.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.

FIG. 1 is a schematic structural diagram of a separating centrifugal well plate provided in an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a base plate according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a cover plate according to an embodiment of the present invention;

FIG. 4 is a schematic structural diagram of an outer tube according to an embodiment of the present invention;

FIG. 5 is a schematic view of the internal structure of the inner tube according to the embodiment of the present invention;

FIG. 6 is a schematic view of the internal structure of a sampling tube assembly according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of an internal structure of a separating orifice plate according to an embodiment of the present invention;

FIG. 8 is a schematic view of another internal structure of a separating orifice plate according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of a centrifugal orifice plate assembly according to an embodiment of the present disclosure;

fig. 10 is a schematic structural diagram of a support block according to an embodiment of the present invention.

In the figure:

1-a bottom plate; 2-cover plate; 3-a combined sampling tube; 4-centrifugal pore channel; 5-supporting block

11-a jack; 12-positioning holes; 13-reinforcing ribs;

111-an annular projection; 112-opening;

121-spacer grooves;

21-a through hole; 22-upright post; 23-a multi-jaw configuration;

221-a fastening portion; 222-an extension;

31-a ring-shaped bump; 32-a guide post; 33-a tube cover;

301-opening a hole; 302-filtration membrane; 303-briquetting; 304-a primary tubular body; 305-a hinge piece; 306-ring plate; 307-inner convex strips; 308-external convex strips;

51-block; 52-a top rod; 53-locating piece.

Detailed Description

The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1, fig. 7 and fig. 8, the present invention provides a separating centrifugal orifice plate, which comprises a bottom plate 1 and a cover plate 2 disposed above the bottom plate 1, wherein the bottom plate 1 and the cover plate 2 are detachably connected, and a plurality of centrifugal orifices 4 for placing combined sampling tubes 3 are formed between the bottom plate 1 and the cover plate 2; the bottom plate 1 is recessed downwards along the vertical direction to form a plurality of positioning holes 12, and the lower surface of the cover plate 2 extends downwards to form a vertical column 22 for being inserted into the positioning holes 12.

Through the arrangement mode, a plurality of combined sampling tubes 3 can be respectively placed in a corresponding centrifugal pore channel 4, and finally, a pore plate with continuously-amplified flux is formed, so that the device is suitable for being used conveniently and quickly under a large number of operating environments. The separable connection between the base plate 1 and the cover plate 2 is provided, which is also convenient for the separation operation and improves the operability.

In order to better realize the fit between the positioning hole 12 and the column 22 and further improve the convenience of assembly, the positioning hole 12 may be further defined as a cylindrical hole formed through the base plate 1, and at least one spacing groove 121 is formed in a side surface of the cylindrical hole extending in a horizontal direction as shown in fig. 2 in order to avoid deformation and the like caused by over-tight fit between the column 22 and the positioning hole 12. Of course, the shape of the spacing groove 121 may be arbitrarily selected, for example, it may be long, and the number may be set according to actual needs, for example, in fig. 2, 4 are arranged at equal intervals along the circumferential direction of the cylindrical hole, and the invention is not limited to this.

Further, as shown in fig. 3, the pillar 22 includes a fastening portion 221 and an extension portion 222, which are coaxially disposed from top to bottom and are formed into a cylinder, the fastening portion 221 is closely attached to the cylindrical hole, and a diameter of the extension portion 222 is smaller than a diameter of the fastening portion 221. In a further preferred embodiment, when the cover plate 2 is in a state of covering the bottom plate 1, the bottom of the upright column 22 can be flush with the bottom of the positioning hole 12, meanwhile, when the cover plate is in the working state, the contact part of the fastening part 221 and the positioning hole 12 is tightly attached, and the two are in a small clearance fit state, so that the relative shaking degree between the two in operations such as centrifugation and the like can be effectively reduced; when the bottom plate 1 and the cover plate 2 need to be separated, after the fastening portion 221 is moved upward, the extending portion 222 and the positioning hole 12 are in a large clearance fit state, so that the separation of the two is facilitated.

In a further preferred embodiment, a plurality of insertion holes 11 penetrate through the bottom plate 1 along the vertical direction, a plurality of through holes 21 corresponding to the insertion holes 11 in a one-to-one manner penetrate through the cover plate 2, and the insertion holes 11 and the through holes 21 are matched to form centrifugal ducts 4.

In the preferred embodiment of the present invention, the upper surface of the cover plate 2 extends upwards from the outer periphery of the through hole 21 to form an annular multi-claw structure 23, and the multi-claw structure 23 is used for embedding the tube cover 33 of the combined sampling tube 3. As shown in FIG. 3, the multi-jaw structure 23 can also refer to the jaw structure, so that after the combined sampling tube 3 passes through the multi-jaw structure 23, the tube cover 33 is slightly larger than the diameter of the lower section of the whole combined sampling tube 3, and thus the tube cover 33 is clamped on or below the lower section of the multi-jaw structure 23 after the multi-jaw structure 23 is pushed open, and the nesting effect between the combined sampling tube 3 and the cover plate 2 is effectively improved.

In a further preferred embodiment, as shown in fig. 2, in order to better achieve a stable fit between the whole sampling combination sampling tube 3 and the bottom plate 1, the inner side wall of the insertion hole 11 is formed with an annular projection 111 along the circumferential direction, the annular projection 111 is formed with a plurality of openings 112 along the vertical direction, and the outer surface of the sampling combination tube 3 is formed with an annular projection 31 along the circumferential direction for being fitted under the annular projection 111. With this arrangement, during the process of placing the combined sampling tube 3 into the insertion hole 11, the annular protrusion 111 can be pushed downwards by the annular protrusion 31 and clamped below the annular protrusion 111, so that the combined sampling tube 3 and the bottom plate 11 can be effectively nested.

In order to better improve the stability during the placement process, the side wall of the centrifugal hole 4 is further extended outwards to form a guide groove, and the outer side wall of the combined sampling tube 3 is extended outwards to form a guide post 32 matched with the guide groove. As shown in fig. 4, the guide post 32 and the guide groove are preferably arranged to extend in the vertical direction.

In order to better adapt to the bearing of the clamping force of the bottom plate 1 on the mechanical arm in the automatic operation condition, a plurality of protruding reinforcing ribs 13 are formed on the outer side surface of the bottom plate 1 along the vertical direction.

Of course, in order to better realize the separation of the combined sampling tube 3 and perform effective separation and collection of the centrifugate, in a preferred embodiment of the present invention, as shown in fig. 6, the combined sampling tube may be further configured as an outer tube and an inner tube which are sequentially nested from outside to inside; and the bottom of the outer tube is formed closed; the bottom of the inner tube is opened to form an opening 301, a filter membrane 302 is arranged on the inner section of the inner tube, and a gap is formed between the filter membrane 302 and the bottom of the inner tube. The above arrangement makes the sample carried on the filter membrane 302 in the inner tube not to flow down including the liquid phase part of the sample liquid on the premise of not operating, and after the operation such as centrifugation, the liquid phase part in the sample can leak through the filter membrane and enter the outer tube through the opening 301, and after the outer tube and the inner tube are conveniently disassembled, the obtained centrifugate can be continuously used for other operations.

The filter membrane 302 may be stably disposed in the inner tube in any suitable manner, for example, a pressing block 303 is further disposed on the filter membrane 302, the pressing block 303 is formed into a through ring structure, and the pressing block 303 is tightly attached to the inner side wall of the inner tube.

In a further preferred embodiment, as shown in fig. 5, the inner tube includes a main tube 304 having an open upper end, and a tube cover 33 connected by a hinge piece 305 for covering the open upper end of the main tube 304, and the middle of the tube cover 33 extends through the main tube 304, and the upper end of the outer circumferential surface of the through portion of the tube cover 33 extends upward to form a protruding ring piece 306. Through the setting of outstanding ring piece 306 for the outstanding setting is realized to the opening, thereby makes its surface be convenient for seal the membrane, and improves and seal the membrane effect.

An inner protrusion 307 is formed on the inner side wall of the main pipe body 304 in the circumferential direction, and an outer protrusion 308 capable of being fitted under the inner protrusion 307 is formed on the outer side surface of the pipe cover 33 covering the inside of the main pipe body 304 in the circumferential direction. The arrangement of the inner protrusion 307 and the outer protrusion 308 may be provided with reference to the arrangement of the annular protrusion 111 and the annular protrusion 31, although the invention is not limited thereto, and similar structures may be used herein.

A stop plate is formed on the upper end surface of the main tube 304 in a horizontal direction, and at least part of the upper end surface of the outer tube is attached to the lower surface of the stop plate.

Further, in order to further reduce the problem that the vibration causes the vibration in the centrifugal process to shake up and down, the fastening portion 221 can be formed with a small triangular structure along the side surface extending outward, the positioning hole 12 is provided with a chamfer groove matched with the positioning hole, and the chamfer block can be inserted into the chamfer groove to limit the shake up and down. Meanwhile, the vertical edge of the chamfering block connected with the fastening part 221 through the shaft is close to the fastening part 221, and the diameter of the shaft is smaller than the vertical height of the chamfering block, so that the chamfering block is convenient to be extruded into the chamfering groove and is convenient to be taken out in a breaking-off manner when not in use.

In another aspect of the embodiment of the present invention, there is also provided a centrifugal orifice plate assembly, as shown in fig. 9, including the separating centrifugal orifice plate as described above and a supporting block 5 disposed below the separating centrifugal orifice plate; wherein the content of the first and second substances,

the supporting block 5 comprises a block body 51 and ejector rods 52 arranged on the upper surface of the block body 51, and the ejector rods 52 correspond to the positioning holes 12 one by one and are inserted into the positioning holes 12;

the sum of the height of the upright post 22 and the height of the ejector rod 52 is greater than the height of the positioning hole 12.

Through the arrangement, the push rods 52 are correspondingly arranged below the positioning holes 12, the upright posts 22 in the positioning holes 12 can be pushed upwards through the upward movement or extension of the push rods 52, the bottom plate 1 and the cover plate 2 are separated, and the self-separation effect is achieved.

In a further preferred embodiment, in order to better improve the matching effect between the supporting block 5 and the base plate 1, so that the positioning hole 12 and the top bar 52 can be directly aligned when placed, as shown in fig. 10, the upper surface of the supporting block 5 is further formed with a plurality of positioning blocks 53 engaged with the outer circumferential surface of the base plate 1 in the circumferential direction.

In another aspect of the embodiments of the present invention, there is also provided a self-separation method of a centrifugal orifice plate, using the centrifugal orifice plate assembly as described above, the self-separation method including:

s10, combining the separating centrifugal pore plates and placing the combined separating centrifugal pore plates in a centrifuge for centrifugation;

s20, placing the separated centrifugal orifice plate after centrifugation on the supporting block 5, and enabling the push rods 52 to be arranged below the positioning holes 12 in a one-to-one correspondence manner;

and S30, ejecting the upright post 22 through the ejector rod 52, and separating the bottom plate 1 from the top plate 2.

This is further illustrated by the following preferred embodiments:

after the bottom plate 1 and the cover plate 2 are matched and inserted with the combined sampling tube 3, the combined sampling tube is placed in a plate centrifuge through an automatic gripper, the cover plate 2 props against the upright post 22 through a plate on the centrifuge to move upwards, and drives the inner tube in the combined sampling tube 3 to be separated from the outer tube together, at the moment, the upright post 22 and the positioning hole 12 are tightly matched, at the moment, the reagent cannot flow downwards due to the filter membrane 302 in the inner tube, the reagent in the inner tube can flow into the outer tube through centrifugation, then the whole separated centrifugal orifice plate is placed on the supporting block 5 through the automatic gripper, the upright post 22 is upwards propped by the ejector rod 52 on the supporting block 5, the part with the smaller diameter at the lower end of the upright post 22 is loosely matched with the positioning hole 12, the cover plate 2 with the inner tube is grabbed by the.

In another aspect of the embodiments of the present invention, there is also provided another self-separation method of a centrifugal orifice plate, including:

s100, placing the supporting block 5 in a centrifuge;

s200, the separated centrifugal hole plates are correspondingly placed on the supporting blocks 5, and the ejector rods 52 are correspondingly arranged below the positioning holes 12 one by one, so that loose matching of the bottom plate 1 and the cover plate 2 is realized;

and S300, after centrifugation, separating the bottom plate 1 from the cover plate 2.

As such, the preferred embodiments are further described:

placing a supporting block 5 in a flat centrifuge, after a bottom plate 1 and a cover plate 2 are matched and arranged in a combined sampling tube 3, placing the combined sampling tube on the placing block 5 in the flat centrifuge through an automatic gripper, supporting the cover plate 2 to move upwards through an ejector rod 52, driving an inner tube in the combined sampling tube 3 to be separated from an outer tube in the bottom plate 1, loosely matching a small-diameter part at the lower end of an upright post 22 of the cover plate 2 with a positioning hole 12, and preventing a reagent from flowing downwards due to a filter membrane 302 in the inner tube; after centrifugation, the reagent in the inner tube can flow into the outer tube in a centrifugal mode, then the cover plate 2 with the inner tube is grabbed by the automatic grabbing hand and discarded, and the bottom plate 1 with the reagent at the bottom can be subjected to subsequent automatic operation.

Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.