阅读说明：本技术 响应面法优化酸枣仁油提取制备工艺 (Optimized wild jujube kernel oil extraction and preparation process by response surface method ) 是由 龚千锋 于欢 严丽萍 翟莹莹 于 2019-11-19 设计创作，主要内容包括：本发明公开了响应面法优化酸枣仁油提取制备工艺,该酸枣仁油提取制备工艺具体步骤如下：利用打粉机对干燥酸枣仁进行粉碎,粉碎后的干燥酸枣仁通过目筛进行筛选,得到酸枣仁粉,并将其装于真封袋中冷藏备用；利用电子天平称取10g酸枣仁粉；以石油醚作为溶剂,利用索氏提取仪连续提取一段时间,得到石油醚提取液；利用旋转蒸发器对S3中得到的石油醚提取液进行旋蒸除去溶剂,得到酸枣仁油；计算酸枣仁油得率。本发明所述的响应面法优化酸枣仁油提取制备工艺,采用Box－Behnken试验原理对酸枣仁油提取工艺进行优化,最终得出酸枣仁油最优提取工艺条件为提取时间6.9h、提取温度82.6℃、料液比1∶16.5,在优化条件下,酸枣仁油得率达到32.21%,与理论值吻合。(The invention discloses a response surface method optimized wild jujube kernel oil extraction preparation process, which comprises the following specific steps: crushing the dried spina date seeds by using a powder grinding machine, screening the crushed dried spina date seeds by using a sieve to obtain spina date seed powder, and filling the spina date seed powder into a vacuum bag for cold storage for later use; weighing 10g of wild jujube kernel powder by using an electronic balance; continuously extracting for a period of time by using a Soxhlet extractor by using petroleum ether as a solvent to obtain a petroleum ether extracting solution; performing rotary evaporation on the petroleum ether extracting solution obtained in the step S3 by using a rotary evaporator to remove a solvent, so as to obtain the wild jujube kernel oil; and calculating the yield of the wild jujube kernel oil. The invention relates to a response surface method optimized wild jujube seed oil extraction preparation process, which optimizes the wild jujube seed oil extraction process by adopting a Box-Behnken test principle to finally obtain the wild jujube seed oil with the optimal extraction process conditions of extraction time of 6.9h, extraction temperature of 82.6 ℃ and material-liquid ratio of 1: 16.5, wherein under the optimal conditions, the yield of the wild jujube seed oil reaches 32.21 percent and is consistent with a theoretical value.)

1. The method for optimizing the wild jujube kernel oil extraction preparation process by the response surface method is characterized by comprising the following specific steps:

s1, raw material pretreatment: crushing the dried spina date seeds by using a powder grinding machine, screening the crushed dried spina date seeds by using a sieve to obtain spina date seed powder, and filling the spina date seed powder into a vacuum bag for cold storage for later use;

s2, weighing the following raw materials: weighing 10g of wild jujube kernel powder by using an electronic balance;

s3, extracting petroleum ether extract: continuously extracting for a period of time by using a Soxhlet extractor by using petroleum ether as a solvent to obtain a petroleum ether extracting solution;

s4, rotary steaming: performing rotary evaporation on the petroleum ether extracting solution obtained in the step S3 by using a rotary evaporator to remove a solvent, so as to obtain the wild jujube kernel oil;

s5, weighing, and calculating the yield of the wild jujube kernel oil.

2. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 1, characterized in that: and S1, the powder grinding machine is a high-speed traditional Chinese medicine grinding machine, the mesh number of the mesh sieve is 40, and the rotating speed of the high-speed traditional Chinese medicine grinding machine is 120 r/min.

3. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 1, characterized in that: the specific steps of extracting the petroleum ether extracting solution by the Soxhlet extractor in the step S3 are as follows:

(1) cutting the filter paper into a rectangle of 8cm multiplied by 15cm, rolling the rectangle into a cylinder with the diameter of 6cm, sealing the bottom of the cylinder, and putting absorbent cotton;

(2) putting the wild jujube kernel powder in the step S2 into a cylinder with the diameter of 6cm, putting the filter paper cylinder into a Soxhlet extraction instrument, heating and refluxing the wild jujube kernel powder in an HH-6 digital display constant-temperature water bath kettle by using petroleum ether, and putting fat in the wild jujube kernel powder into a solvent to obtain a petroleum ether extracting solution.

4. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 3, characterized in that: and in the step S3, the extraction time of the petroleum ether extracting solution extracted by the Soxhlet extractor is 2-10 h.

5. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 3, characterized in that: the temperature of the HH-6 digital display constant temperature water bath in the step (2) is controlled to be 70-90 ℃.

6. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 1, characterized in that: the ratio of the feed liquid in the step (2) is controlled to be 1:19-1: 23.

7. The response surface method optimized extraction and preparation process of spine date seed oil according to claim 1, characterized in that: the yield of the wild jujube kernel oil in the step S5 can be obtained by the formulaAnd (4) calculating.

Technical Field

The invention relates to the technical field of wild jujube kernel oil extraction, in particular to a response surface method optimized wild jujube kernel oil extraction preparation process.

Background

The process for extracting the wild jujube kernel oil by using the petroleum ether as an extraction solvent is researched. The extraction time, the extraction temperature and the material-liquid ratio are used as influencing factors, on the basis of a single-factor test, the yield of the wild jujube kernel oil is used as a response value, a Box-Behnken response surface method optimization test with three factors and three levels is carried out, the wild jujube is a common heart-nourishing tranquilizer, is called as an eastern sleeping fruit, belongs to the first medicinal and edible dual-purpose product issued by the ministry of health, and is mainly produced in Liaoning, Hebei, Shanxi and the like. The oil content of the spina date seed oil can reach 32 percent, the spina date seed oil is nontoxic in clinical application and free of tolerance phenomenon in long-term application, and the oil contains unsaturated fatty acid, squalene, sterol, vitamin E and other components and has the functions of calming, hypnotizing, reducing blood fat, resisting inflammation, resisting oxidation, resisting bacteria, resisting tumors, enhancing learning and memory and the like;

the wild jujube seed oil extraction and preparation process takes petroleum ether as an extraction solvent, but the traditional wild jujube seed oil extraction and preparation process cannot well determine the influence factors of the yield of the wild jujube seed oil, so that the yield of the wild jujube seed oil is low, the wild jujube seed oil cannot be fully extracted, raw materials are wasted, and the cost for extracting the wild jujube seed oil is increased.

Disclosure of Invention

The invention mainly aims to provide a response surface method optimized wild jujube seed oil extraction and preparation process, which can effectively solve the problems that the wild jujube seed oil extraction and preparation process in the background art takes petroleum ether as an extraction solvent, but the traditional wild jujube seed oil extraction and preparation process cannot well determine the influence factors of the wild jujube seed oil yield, so that the wild jujube seed oil yield is low, the full extraction cannot be carried out, the raw materials are wasted, and the cost for extracting the wild jujube seed oil is increased.

In order to achieve the purpose, the invention adopts the technical scheme that:

the method optimizes the wild jujube kernel oil extraction and preparation process by a response surface method, and the wild jujube kernel oil extraction and preparation process comprises the following specific steps:

s1, raw material pretreatment: crushing the dried spina date seeds by using a powder grinding machine, screening the crushed dried spina date seeds by using a sieve to obtain spina date seed powder, and filling the spina date seed powder into a vacuum bag for cold storage for later use;

s2, weighing the following raw materials: weighing 10g of wild jujube kernel powder by using an electronic balance;

s3, extracting petroleum ether extract: continuously extracting for a period of time by using a Soxhlet extractor by using petroleum ether as a solvent to obtain a petroleum ether extracting solution;

s4, rotary steaming: performing rotary evaporation on the petroleum ether extracting solution obtained in the step S3 by using a rotary evaporator to remove a solvent, so as to obtain the wild jujube kernel oil;

s5, weighing, and calculating the yield of the wild jujube kernel oil.

As a further scheme of the invention: and S1, the powder grinding machine is a high-speed traditional Chinese medicine grinding machine, the mesh number of the mesh sieve is 40, and the rotating speed of the high-speed traditional Chinese medicine grinding machine is 120 r/min.

As a further scheme of the invention: the specific steps of extracting the petroleum ether extracting solution by the Soxhlet extractor in the step S3 are as follows:

(1) cutting the filter paper into a rectangle of 8cm multiplied by 15cm, rolling the rectangle into a cylinder with the diameter of 6cm, sealing the bottom of the cylinder, and putting absorbent cotton;

(2) putting the wild jujube kernel powder in the step S2 into a cylinder with the diameter of 6cm, putting the filter paper cylinder into a Soxhlet extraction instrument, heating and refluxing the wild jujube kernel powder in an HH-6 digital display constant-temperature water bath kettle by using petroleum ether, and putting fat in the wild jujube kernel powder into a solvent to obtain a petroleum ether extracting solution.

As a further scheme of the invention: and in the step S3, the extraction time of the petroleum ether extracting solution extracted by the Soxhlet extractor is 2-10 h.

As a further scheme of the invention: the temperature of the HH-6 digital display constant temperature water bath in the step (2) is controlled to be 70-90 ℃.

As a further scheme of the invention: the ratio of the feed liquid in the step (2) is controlled to be 1:19-1: 23.

As a further scheme of the invention: the yield of the wild jujube kernel oil in the step S5 can be obtained by the formulaAnd (4) calculating.

Compared with the prior art, the invention has the following beneficial effects: on the basis of a single-factor test, 3 factors of extraction time, extraction temperature and material-liquid ratio are determined, the wild jujube seed oil yield (Y) is taken as a response value, a Box-Behnken test principle is adopted to optimize the wild jujube seed oil extraction process, response surface analysis is adopted to optimize the wild jujube seed oil extraction process, and finally, the optimal extraction process conditions of the wild jujube seed oil are that the extraction time is 6.9h, the extraction temperature is 82.6 ℃, and the material-liquid ratio is 1: 16.5, and under the optimized conditions, the wild jujube seed oil yield reaches 32.21% and is consistent with the theoretical value.

Drawings

FIG. 1 is a graph showing the effect of extraction time on yield of wild jujube kernel oil;

FIG. 2 is a graph showing the effect of extraction temperature on the yield of wild jujube kernel oil;

FIG. 3 is a graph showing the effect of feed liquid ratio on the yield of wild jujube kernel oil.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.