阅读说明：本技术 具有降血脂、改善胃肠道功能的组合物及其制备方法 (Composition with functions of reducing blood fat and improving gastrointestinal tract and preparation method thereof ) 是由 贾福怀 陶刚 袁媛 王彩霞 许璐云 熊菲菲 王俊 戴飞 陈磊 陆伟 于 2019-05-05 设计创作，主要内容包括：本发明涉及一种具有降血脂、改善胃肠道功能的组合物及其制备方法,本发明组合物主要聚焦两方面的功效,其一是降血脂的功效,其二是改善胃肠道功能的功效。针对降血脂这一功效,主要通过将马齿苋制成微囊与同样具有降血脂、调节血压功能的山楂果粉、红曲米粉、玉米低聚肽配伍,显著提升了其调节血脂平衡,降低血液中胆固醇及甘油三酯含量的功能。针对改善胃肠道功能的功效,我们将鸡冠花、委陵菜制成提取物与富含膳食纤维的聚葡萄糖、魔芋粉、葛根粉配伍,其对胃肠道的刺激较小,能起到温和滋补、润肠通便、清热解毒、止血消肿去溃疡、调节肠道菌群平衡、改善胃肠道功能的奇效。(The invention relates to a composition with the functions of reducing blood fat and improving gastrointestinal tract function and a preparation method thereof. Aiming at the efficacy of reducing blood fat, the purslane is mainly prepared into the microcapsule to be matched with hawthorn fruit powder, red yeast rice powder and corn oligopeptide which also have the functions of reducing blood fat and regulating blood pressure, so that the functions of regulating blood fat balance and reducing the contents of cholesterol and triglyceride in blood are obviously improved. Aiming at the effect of improving the gastrointestinal function, the cockscomb extract and the potentilla chinensis extract are combined with the polydextrose, the konjac powder and the pueraria powder which are rich in dietary fibers, so that the stimulation to the gastrointestinal tract is small, and the special effects of mildness and nourishing, bowel relaxing, heat clearing and detoxifying, hemostasis and detumescence and ulcer removal, intestinal flora balance regulation and gastrointestinal function improvement can be achieved.)

1. A composition with the functions of reducing blood fat and improving gastrointestinal tract function is characterized in that: according to parts by weight, comprises

2. The composition for reducing blood lipid and improving gastrointestinal tract function according to claim 1, wherein: according to parts by weight, comprises

3. A method for preparing the composition for reducing blood lipid and improving gastrointestinal tract function according to claim 1 or 2, wherein the composition comprises the following steps: comprises the following steps

(1) The corn protein powder is sequentially subjected to size mixing, enzymolysis, centrifugal filtration, reduced pressure concentration and spray drying to obtain the required corn oligopeptide, wherein the oligopeptide component with the molecular weight of less than 1000D in the corn oligopeptide accounts for more than 95 percent;

(2) respectively extracting flos Celosiae Cristatae and herba Potentillae chinensis with ethanol under reflux, concentrating under reduced pressure, vacuum freeze drying, and pulverizing to obtain flos Celosiae Cristatae and herba Potentillae chinensis extract;

(3) pretreating herba Portulacae with coarse powder, and adding supercritical CO₂CO extraction in an extraction plant₂Supercritical extraction, emulsifying the obtained extract, mixing with wall material solution, homogenizing,spray drying to obtain the required purslane microcapsule, wherein the embedding rate of active ingredients in the purslane microcapsule is more than 90%, and the particle size of the microcapsule is 50-260 mu m;

(4) respectively carrying out low-temperature superfine grinding, membrane separation and vacuum microwave drying on Plantago ovata forsk shells and chia seeds in sequence to obtain Plantago ovata forsk shell dietary fibers and chia seed dietary fibers, wherein the content of soluble dietary fibers and insoluble dietary fibers is more than 93%;

(5) vacuum freeze drying and low-temperature superfine grinding are carried out on hawthorn fruits to obtain hawthorn fruit powder, and 100 percent of the hawthorn fruit powder can pass through a 300-mesh sieve;

(6) uniformly mixing the prepared plantain seed husk dietary fiber, chia seed dietary fiber, hawthorn fruit powder, kudzu root powder, polydextrose and konjac powder according to the mass parts of the components to obtain a mixture, adding corn oligopeptide, red yeast rice powder, cockscomb extract, potentilla chinensis extract and purslane microcapsule into the mixture, and continuously stirring for 10-30 min to obtain the composition.

4. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 3, wherein the composition comprises the following components: the corn oligopeptide in the step (1) is prepared by the following method

(a) Size mixing: sieving corn protein powder with a 40-80-mesh sieve, preparing a corn protein solution by using purified water, and uniformly mixing the corn protein solution; adding purified water into the uniformly mixed zein solution to obtain a dispersion liquid; adjusting the pH of the dispersion liquid of the corn protein powder to 6-8;

(b) enzymolysis: heating the dispersion liquid of the corn protein powder to 50 ℃, keeping the temperature at 50 +/-5 ℃, adding neutral protease and papain into the dispersion liquid of the corn protein powder, uniformly stirring and performing enzymolysis for 1-3 hours, wherein the addition amount of the neutral protease is 0.3-0.5% of the content of the substrate, the addition amount of the papain is 0.1-0.4% of the content of the substrate, and performing enzyme deactivation by using a high-temperature instant enzyme deactivation method after the enzymolysis is finished;

(c) centrifugal filtration: carrying out centrifugal separation on the enzyme-inactivated enzymolysis liquid by a centrifugal machine at 13000-16000 rpm, removing impurities, collecting clear liquid, starting membrane filtration equipment, filtering the clear liquid by a microporous filter membrane, wherein the thickness of the filter membrane is 60-120 mu m, the aperture of the filter membrane is 3-6 mu m, the operating pressure is 0.01-0.25 MPa, ensuring that the filtrate is clear and transparent, and removing filter residues; passing the filtered filtrate through an ion exchange column and an active carbon column, performing desalination and decolorization treatment, and removing free amino acid by nanofiltration;

(d) and (3) concentrating under reduced pressure: carrying out vacuum reduced pressure concentration on the solution after ion exchange, wherein the concentration temperature is 60-80 ℃, and the vacuum degree is-0.045-0.085 Mpa, so as to obtain an oligopeptide concentrated solution, and the relative density is controlled to be 1.05-1.09;

(e) spray drying: and (3) carrying out spray drying on the oligopeptide concentrated solution, wherein the process parameters are that the nozzle temperature is 165-185 ℃, the outlet temperature is 80-92 ℃, and the feeding speed is 1.8-2.8L/min, so that the corn oligopeptide meeting the quality requirement is obtained.

5. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 3, wherein the composition comprises the following components: the cockscomb extract and potentilla chinensis extract in the step (2) can be prepared by the following steps

(a) Alcohol reflux extraction: taking the crushed cockscomb or potentilla chinensis, adding 50-70% ethanol (v/v) which is 6-8 times of the weight of the materials, carrying out reflux extraction for 2-4 times at 80-92 ℃ for 1 hour each time, filtering and combining the extracting solution;

(b) and (3) concentrating under reduced pressure: concentrating the extracting solution under reduced pressure, and recovering the solvent, wherein the concentration temperature is controlled to be 45-65 ℃, the vacuum degree is controlled to be-0.06-0.09 Mpa, and the relative density is controlled to be 1.12-1.20, so as to obtain an extract;

(c) vacuum freeze drying: putting the extract into vacuum freeze drying equipment for drying, and setting parameters as follows: the pre-freezing initial temperature is-35 to-45 ℃, the pre-freezing speed is 0.2 to 0.5 ℃/min, the pre-freezing end point temperature is-65 ℃, the pressure of a drying chamber is 40 to 90pa, the temperature of a heating plate is 35 to 42 ℃, and the drying time is 16 to 24 hours;

(d) crushing: pulverizing the dried extract, and sieving with 100 mesh sieve.

6. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 3, wherein the composition comprises the following components: the preparation method of the purslane microcapsule in the step (3) is as follows

(a) Pulverizing herba Portulacae into coarse powder, adding CO₂Extracting by a supercritical extraction method at the extraction temperature of 32-42 ℃ and the extraction pressure of 28-35 Mpa in CO₂The flow rate is 8-14 kg/h, and the extraction time is 120-180 min, so that a purslane extract is obtained;

(b) weighing maltodextrin and gamma-cyclodextrin, stirring and dissolving the maltodextrin and the gamma-cyclodextrin in hot water at 85 ℃ to obtain a wall material solution, wherein the total mass fraction of the maltodextrin and the gamma-cyclodextrin in the wall material solution is 5-10%, and the mass ratio of the maltodextrin to the gamma-cyclodextrin is 1: 1-3;

(c) adding the purslane extract into a 10% ethanol solution containing sucrose ester and phospholipid, wherein the mass ratio of the sucrose ester to the phospholipid is 1: 1-4, and the total mass fraction of the sucrose ester and the phospholipid in the ethanol solution is 1-3% to prepare a purslane emulsion with the mass fraction of 6-8%;

(d) pouring the purslane emulsion prepared in the step (c) into the wall material solution prepared in the step (b), wherein the volume ratio of the purslane emulsion to the wall material solution is 0.8-1: 1, and carrying out emulsification and homogenization treatment at 50 ℃ to prepare an initial product;

(e) and (3) carrying out spray drying on the prepared initial product, wherein the inlet temperature of the spray drying is 150-175 ℃, the outlet temperature of the spray drying is 80-90 ℃, and the feeding speed is 0.5-1.5L/min, so as to prepare the required purslane microcapsule finished product.

7. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 3, wherein the composition comprises the following components: the plantain seed husk dietary fiber and chia seed dietary fiber in the step (4) can be prepared by the following preparation method

(a) And (3) low-temperature superfine grinding: putting Plantago ovata seed husk or chia seed into low-temperature superfine grinding equipment for superfine grinding, and sieving with a 500-mesh sieve to obtain Plantago ovata seed husk superfine powder or chia seed superfine powder;

(b) enzymolysis: putting Plantago ovata husk superfine powder or chia seed superfine powder into purified water of 8-10 times, uniformly stirring to prepare a dispersion, heating to raise the temperature and keep the temperature at 50 +/-2 ℃, adding alpha-amylase into the dispersion, uniformly stirring for enzymolysis for 0.5-1.5 h, wherein the addition amount of the alpha-amylase is 0.4-0.6% of the content of a substrate, adjusting the pH value to 2.5-3.5, adding acidic protease and pectinase into the dispersion, uniformly stirring for enzymolysis for 2-4 h, wherein the addition amount of the acidic protease is 0.5-0.8% of the content of the substrate, the addition amount of the pectinase is 0.3-0.6% of the content of the substrate, and inactivating the enzyme by using a high-temperature instant enzyme inactivating method after the enzymolysis is finished;

(c) alcohol dissolving: adding edible ethanol accounting for 80-120% of the total weight of the enzymolysis liquid into the enzymolysis liquid, keeping the temperature at 60 +/-5 ℃, continuously stirring for 20-40 min, dissolving various fat-soluble components contained in insoluble suspended matters in the enzymolysis liquid, and performing centrifugal treatment on the enzymolysis liquid to obtain part of dietary fibers;

(d) membrane separation: concentrating the enzymolysis liquid under vacuum and reduced pressure to recover the solvent, concentrating until the relative density is 1.01-1.02, filtering by a membrane separation technology to obtain another part of dietary fiber solution with the molecular weight obviously different from other nutrients, wherein the thickness of a filter membrane is 80-150 mu m, the pore diameter of the filter membrane is 2-8 mu m, and the operating pressure is 0.12-0.25 MPa; concentrating the dietary fiber solution under vacuum and reduced pressure to obtain a concentrated solution with the relative density of 1.15-1.25;

(e) vacuum microwave drying: collecting the dietary fiber concentrated solution obtained by membrane separation and the dietary fiber obtained by centrifugation, and placing the dietary fiber concentrated solution and the dietary fiber obtained by centrifugation in a vacuum microwave drying device for drying treatment, wherein the drying condition is a microwave vacuum drying stage, the vacuum pressure is 3.0-5.5kpa, the microwave power density is 0.75-0.85W/g, and the water content is controlled to be 2-4%, so that the psyllium husk dietary fiber and chia seed dietary fiber are finally obtained.

8. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 3, wherein the composition comprises the following components: the preparation method of the hawthorn fruit powder in the step (5) is as follows

(a) Vacuum freeze drying: slicing hawthorn fruits, controlling the average thickness of the hawthorn fruit slices to be 2-4 mm, putting the hawthorn fruit slices into vacuum freeze drying equipment for drying, setting the parameters to be 0.4-0.6 ℃/min of pre-freezing rate, the pre-freezing initial temperature of-30-45 ℃, the pre-freezing final temperature of-70 ℃, the pressure of a drying chamber of 55-85 pa, the temperature of a heating plate of 38-45 ℃ and the drying time of 20-28 h;

(b) and (3) low-temperature superfine grinding: and (3) putting the dried hawthorn freeze-dried slices into a low-temperature ultrafine pulverizer for low-temperature ultrafine grinding, wherein 100% of the obtained hawthorn fruit powder is required to pass through a 300-mesh sieve.

9. The method for preparing the composition for reducing blood fat and improving gastrointestinal tract function according to claim 4, wherein the composition comprises the following components: the neutral protease is selected from bacillus subtilis neutral protease, and the enzyme activity is more than or equal to 20 ten thousand U/g.

Technical Field

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a composition with functions of reducing blood fat and improving gastrointestinal tract and a preparation method thereof.

Background

The gastrointestinal tract is used as a digestive organ of a human body, occupies an irreplaceable important position in the metabolism of organism substances, has the functions of secretion, excretion, biotransformation and the like, and can secrete various digestive fluids to participate in the digestive absorption process of food. The intestinal microbial flora such as bifidobacterium, lactobacillus and the like can synthesize various vitamins necessary for the growth and development of human bodies, can also synthesize necessary amino acids by utilizing protein residues, participate in the metabolism of saccharides and proteins, and simultaneously promote the absorption of mineral elements, and the nutrient substances have important effects on the health of human bodies. 10 trillion bacteria parasitize in human intestinal tract, which can affect weight and digestion ability, resist infection of pathogenic bacteria and risk of autoimmune disease, promote gastrointestinal tract peristalsis, prevent constipation and diarrhea, promote discharge of harmful substances, and maintain the normal physiological functions of host such as nutrition, immunity and digestion.

The change of the stress emotion directly influences the change of organs of the human body, the most sensitive expression is the gastrointestinal tract, and the adverse emotion can cause the dysfunction of the hypothalamus through the cerebral cortex and influence the gastrointestinal tract function to cause the gastrointestinal tract dysfunction. Irregular diet for a long time, large amount of wine and irritant food, overeating or eating too soon, which can cause gastrointestinal peristalsis dysfunction, irregular gastrointestinal fluid secretion, gastrointestinal mucosa damage and intestinal microecological imbalance. Digestive system diseases exist originally, gastrointestinal disorder occurs, gastrointestinal dysfunction is generated by careless diet, dyspepsia, acute gastroenteritis, ulcer, gastrointestinal perforation and the like are caused in long time. In addition, modern people are under heavy work and often work overtime, which easily causes uncontrollable fatigue, immunity reduction and sub-health state which is difficult to relieve due to insufficient blood supply of gastrointestinal tracts.

With the rapid development of social economy, the pace of life is faster and faster, people's dietary structure and living habits are changed in a covered area, and obesity, diabetes, hyperlipidemia, coronary heart disease, colon cancer and other series of civilization diseases caused by unbalanced diet or overnutrition become main diseases harmful to the health of people in China, and even can be said to be the first killer of intestinal health. In large and medium cities, particularly developed economic cities along the coast, people eat more and more refined foods, the content of dietary fibers in the ingested foods is less and less, the daily requirements of normal human bodies on the dietary fibers are difficult to meet, and a large number of scientific researches prove that the dietary fibers are not taken sufficiently, so that the dietary fibers are one of the important reasons for the large and medium cities that the incidence rate of obesity, diabetes, cardiovascular diseases and malignant tumors is greatly increased. In addition, excessive sugar and fat are taken every day, which often causes heavy body, fatigability or obesity and abnormal glycolipid metabolism; meanwhile, the life rhythm of the crowd is very fast, the working pressure is high, a large amount of unreasonable food is taken at wrong time, endocrine disorder is easily caused, and a series of symptoms such as stress diarrhea, constipation, halitosis, pox or color spots and the like appear. Therefore, some products claim to be oil-draining, toxin-expelling and intestine-clearing products appear in the market, but many problems are hidden behind the products, such as the addition of diarrhea-assisting components such as senna leaves and rheum officinale or other prohibited drug components, which can stimulate the intestinal tract and the nerves, have great potential safety hazards to the health of people, and are limited to the applicable people to a certain extent due to strong irritation.

China is a world with gastrointestinal diseases, the number of people with gastrointestinal dysfunction, intestinal microecological imbalance and gastrointestinal organic diseases is continuously increased, and the people with three highs (hyperglycemia, hypertension and hyperlipidemia) are also more and more in China. Traditional gastrointestinal disease drugs such as aluminum hydroxide, magnesium hydroxide, calcium carbonate, sodium bicarbonate and the like cause excessive intake of aluminum, magnesium, calcium, sodium and other components after long-term eating, thereby causing various side effects: patients with hypertension and poor renal function may aggravate the disease condition by eating sodium bicarbonate for a long time; constipation, intestinal obstruction or osteomalacia can be caused by improper use of an aluminum-containing preparation for a long time; calcium carbonate is easy to cause flatulence; a common side effect of magnesium-containing preparations is diarrhea; the long-term administration of the medicine can damage the mucous membrane of the gastrointestinal tract, aggravate the state of the disease and greatly hurt the original qi. Traditional gastrointestinal anti-inflammatory drugs such as norfloxacin, amoxicillin, cephalosporins and the like can cause a series of side effects such as stomach discomfort, abdominal pain, diarrhea, nausea, vomiting and the like, and the use of the anti-inflammatory drugs can cause the drug resistance of germs to be enhanced, and can kill a lot of beneficial bacteria in intestinal tracts and destroy the micro-ecological balance of the intestinal tracts. The traditional lipid-lowering western medicines for treating dyslipidemia mainly comprise: statins (e.g., atorvastatin, rosuvastatin, fluvastatin, simvastatin, pravastatin, pitavastatin, simvastatin, etc.), fibrates (e.g., fenofibrate), cholesterol absorption inhibitors (e.g., ezetimibe), prorococine, bile acid sequestrants, and the like. Adverse reactions of these drugs are: liver dysfunction (mainly manifested by rising of transaminase), muscle adverse reaction (including myalgia, myositis and rhabdomyolysis), and potential risks of increasing new onset diabetes and aggravating metabolic burden of liver and kidney after long-term administration, so that patients with liver and kidney dysfunction are not suitable for taking lipid-lowering drugs.

Disclosure of Invention

The invention aims to solve the technical problem of the prior art and provides a composition with the functions of reducing blood fat and improving gastrointestinal tract, which can achieve the effects of reducing blood fat and improving gastrointestinal tract functions without damaging viscera, mildly relax bowel, relieve constipation, expel toxin, repair gastrointestinal tract mucosa tissues, adjust intestinal tract microecological balance, adjust blood fat balance, reduce the content of cholesterol and triglyceride in blood and furthest reduce or avoid adverse reactions and side effects.

The invention aims to solve another technical problem and provides a preparation method of the composition for reducing blood fat and improving gastrointestinal tract function aiming at the current situation of the prior art, which can furthest retain the main functional components of the medicine, improve the bioavailability of the medicine and improve the medicine effect.

The technical scheme adopted by the invention for solving the technical problems is as follows: a composition with the functions of reducing blood fat and improving gastrointestinal tract function is characterized in that: according to parts by weight, comprises

Preferably, the composition comprises the following components in parts by weight

The psyllium husk dietary fiber and chia seed dietary fiber in the composition have the obvious effects of relaxing bowel, improving gastrointestinal functions, promoting the propagation of beneficial intestinal flora and controlling blood sugar and cholesterol; the purslane microcapsule is derived from traditional Chinese herbal medicines, and can clear away heat and toxic materials, moisten dryness and smooth intestines, inhibit bacteria and prevent ulcer, and regulate blood fat; the cockscomb extract and potentilla chinensis extract are also from traditional Chinese herbal medicines, and can clear away heat and toxic materials, stop bleeding, relieve swelling and resist ulcer, and improve gastrointestinal health; polydextrose and konjac flour enrich sources and varieties of high-quality dietary fibers, regulate intestinal flora balance, relax bowel, regulate lipid metabolism and reduce cholesterol; the hawthorn fruit powder and the red yeast rice powder have the effects of invigorating stomach, promoting digestion, tonifying qi, reducing blood fat and reducing cholesterol; the corn oligopeptide and the kudzu root powder have excellent peculiar effects of resisting oxidation, dispelling the effects of alcohol and protecting the liver. The psyllium husk is the husk of artificially planted psyllium seeds of Plantago of Plantaginaceae, is rich in soluble dietary fiber and insoluble dietary fiber, and can supplement dietary fiber, relax bowel, control weight, reduce cholesterol, and reduce cardiovascular and cerebrovascular diseases risk. Chia seed is seed of herba Menthae plant such as Gordonia euryales, contains abundant dietary fiber, can produce satiety, slow down digestion rate, control blood sugar and cholesterol content, and has effects of loosening bowel to relieve constipation and improving gastrointestinal function.

The fructus crataegi is fruit of Crataegus of Rosaceae, is rich in dietary fiber, has sour and sweet taste and mild nature, and can enter spleen, stomach and liver meridians. Has effects in promoting digestion, invigorating stomach, promoting qi circulation, removing blood stasis, eliminating turbid pathogen, reducing blood lipid, resisting atherosclerosis, inhibiting bacteria, and tonifying heart. Kudzuvine root, radix Puerariae, sweet and pungent in flavor, cool in nature, enters spleen and stomach meridians. Has effects in expelling pathogenic factors from muscles, relieving fever, promoting salivation, quenching thirst, promoting eruption, invigorating yang, relieving diarrhea, dredging meridian passage, relieving alcoholism, and protecting liver. Polydextrose is a water-soluble dietary fiber, is used as a prebiotic, and has effects of regulating intestinal flora balance, regulating lipid metabolism, reducing cholesterol, reducing sugar absorption, preventing and treating constipation, removing toxic substance and caring skin. The konjak has multiple functions of reducing blood sugar and blood fat level, reducing pressure, removing toxin, beautifying, eliminating free radicals in vivo, resisting aging, dredging collaterals, stimulating appetite, losing weight, relaxing bowels, improving immunity and the like. The corn oligopeptide is prepared from corn protein powder as a raw material by processes of enzymolysis, drying and the like, and has the effects of reducing blood pressure, sobering up and protecting liver, enhancing immunity, resisting oxidation, resisting fatigue and the like.

The red yeast rice is prepared by fermenting rice serving as a raw material with monascus purpureus, is rich in natural lovastatin, sweet in taste and warm in nature, and has the effects of tonifying spleen to promote digestion, promoting blood circulation to remove blood stasis, reducing blood fat and treating and preventing cardiovascular and cerebrovascular diseases. Compared with single statin western medicines, the red yeast rice has higher safety and can not cause any damage to the functions of the liver and the kidney in a reasonable dose.

Purslane is the dry aerial part of purslane family, has sour taste and cold nature, and enters liver and large intestine channels. Has effects of clearing heat and detoxicating, cooling blood and stopping bleeding, treating dysentery, diarrhea, enteritis, carbuncle sore, toxic swelling, diminishing inflammation, relieving pain, improving gastrointestinal health, protecting liver, and reducing blood lipid. Omega-3 fatty acids rich in purslane can inhibit the absorption of human body to cholesterol acid, reduce the concentration of blood cholesterol, improve the elasticity of vessel wall, and is very beneficial to preventing and treating cardiovascular diseases.

The flos Celosiae Cristatae is dry inflorescence of flos Celosiae Cristatae of Amaranthaceae, and has sweet, astringent, and cold properties, and enters liver and large intestine meridians. Has effects of dispelling pathogenic wind, clearing away heat, astringing, stopping bleeding, stopping leukorrhagia, stopping dysentery, protecting gastrointestinal tract, and treating ulcer, and can be used for treating hemoptysis, hematemesis, anal fistula, and red and white dysentery.

Potentilla chinensis is dry whole plant of Potentilla chinensis of Rosaceae, is bitter and cold in taste, and enters liver and large intestine channels. Has effects of clearing away heat and toxic materials, cooling blood, stopping bleeding, relieving swelling, removing ulcer, and improving gastrointestinal health, and can be used for treating dysentery with bloody stool, abdominal pain, chronic dysentery, hemorrhoid hemorrhage, carbuncle, swelling, and sore.

A preparation method of the composition with the functions of reducing blood fat and improving gastrointestinal tract is characterized by comprising the following steps: comprises the following steps

(1) The corn protein powder is sequentially subjected to size mixing, enzymolysis, centrifugal filtration, reduced pressure concentration and spray drying to obtain the required corn oligopeptide, wherein the oligopeptide component with the molecular weight of less than 1000D in the corn oligopeptide accounts for more than 95 percent;

(2) respectively extracting flos Celosiae Cristatae and herba Potentillae chinensis with ethanol under reflux, concentrating under reduced pressure, vacuum freeze drying, and pulverizing to obtain flos Celosiae Cristatae and herba Potentillae chinensis extract;

(3) pretreating herba Portulacae with coarse powder, and adding supercritical CO₂CO extraction in an extraction plant₂Performing supercritical extraction, emulsifying the obtained extract, mixing the extract with a wall material solution, homogenizing, and performing spray drying to obtain the required purslane microcapsule, wherein the embedding rate of the active ingredients in the purslane microcapsule is above 90%, and the particle size of the microcapsule is 50-260 mu m;

(4) respectively carrying out low-temperature superfine grinding, membrane separation and vacuum microwave drying on Plantago ovata forsk shells and chia seeds in sequence to obtain Plantago ovata forsk shell dietary fibers and chia seed dietary fibers, wherein the content of soluble dietary fibers and insoluble dietary fibers is more than 93%;

(5) vacuum freeze drying and low-temperature superfine grinding are carried out on hawthorn fruits to obtain hawthorn fruit powder, and 100 percent of the hawthorn fruit powder can pass through a 300-mesh sieve;

(6) uniformly mixing the prepared plantain seed husk dietary fiber, chia seed dietary fiber, hawthorn fruit powder, kudzu root powder, polydextrose and konjac powder according to the mass parts of the components to obtain a mixture, adding corn oligopeptide, red yeast rice powder, cockscomb extract, potentilla chinensis extract and purslane microcapsule into the mixture, and continuously stirring for 10-30 min to obtain the composition.

Preferably, the corn oligopeptide in the step (1) is prepared by the following method

(a) Size mixing: sieving corn protein powder with a 40-80-mesh sieve, preparing a corn protein solution by using purified water, and uniformly mixing the corn protein solution; adding purified water into the uniformly mixed zein solution to obtain a dispersion liquid; adjusting the pH of the dispersion liquid of the corn protein powder to 6-8;

(b) enzymolysis: heating the dispersion liquid of the corn protein powder to 50 ℃, keeping the temperature at 50 +/-5 ℃, adding neutral protease and papain into the dispersion liquid of the corn protein powder, uniformly stirring and performing enzymolysis for 1-3 hours, wherein the addition amount of the neutral protease is 0.3-0.5% of the content of the substrate, the addition amount of the papain is 0.1-0.4% of the content of the substrate, and performing enzyme deactivation by using a high-temperature instant enzyme deactivation method after the enzymolysis is finished;

(c) centrifugal filtration: carrying out centrifugal separation on the enzyme-inactivated enzymolysis liquid by a centrifugal machine at 13000-16000 rpm, removing impurities, collecting clear liquid, starting membrane filtration equipment, filtering the clear liquid by a microporous filter membrane, wherein the thickness of the filter membrane is 60-120 mu m, the aperture of the filter membrane is 3-6 mu m, the operating pressure is 0.01-0.25 MPa, ensuring that the filtrate is clear and transparent, and removing filter residues; passing the filtered filtrate through an ion exchange column and an active carbon column, performing desalination and decolorization treatment, and removing free amino acid by nanofiltration;

(d) and (3) concentrating under reduced pressure: carrying out vacuum reduced pressure concentration on the solution after ion exchange, wherein the concentration temperature is 60-80 ℃, and the vacuum degree is-0.045-0.085 Mpa, so as to obtain an oligopeptide concentrated solution, and the relative density is controlled to be 1.05-1.09;

(e) spray drying: and (3) carrying out spray drying on the oligopeptide concentrated solution, wherein the process parameters are that the nozzle temperature is 165-185 ℃, the outlet temperature is 80-92 ℃, and the feeding speed is 1.8-2.8L/min, so that the corn oligopeptide meeting the quality requirement is obtained.

Preferably, the cockscomb extract and potentilla chinensis extract in the step (2) can be prepared by the following steps

(a) Alcohol reflux extraction: taking the crushed cockscomb or potentilla chinensis, adding 50-70% ethanol (v/v) which is 6-8 times of the weight of the materials, carrying out reflux extraction for 2-4 times at 80-92 ℃ for 1 hour each time, filtering and combining the extracting solution;

(b) and (3) concentrating under reduced pressure: concentrating the extracting solution under reduced pressure, and recovering the solvent, wherein the concentration temperature is controlled to be 45-65 ℃, the vacuum degree is controlled to be-0.06-0.09 Mpa, and the relative density is controlled to be 1.12-1.20, so as to obtain an extract;

(c) vacuum freeze drying: putting the extract into vacuum freeze drying equipment for drying, and setting parameters as follows: the pre-freezing initial temperature is-35 to-45 ℃, the pre-freezing speed is 0.2 to 0.5 ℃/min, the pre-freezing end point temperature is-65 ℃, the pressure of a drying chamber is 40 to 90pa, the temperature of a heating plate is 35 to 42 ℃, and the drying time is 16 to 24 hours;

(d) crushing: pulverizing the dried extract, and sieving with 100 mesh sieve.

Preferably, the preparation method of the purslane microcapsules in the step (3) is as follows

(a) Pulverizing herba Portulacae into coarse powder, adding CO₂Extracting by supercritical extraction at 32-42 deg.C under 28-35 Mpa of CO₂The flow rate is 8-14 kg/h, and the extraction time is 120-180 min, so that a purslane extract is obtained;

(b) weighing maltodextrin and gamma-cyclodextrin, stirring and dissolving the maltodextrin and the gamma-cyclodextrin in hot water at 85 ℃ to obtain a wall material solution, wherein the total mass fraction of the maltodextrin and the gamma-cyclodextrin in the wall material solution is 5-10%, and the mass ratio of the maltodextrin to the gamma-cyclodextrin is 1: 1-3;

(c) adding the purslane extract into a 10% ethanol solution containing sucrose ester and phospholipid, wherein the mass ratio of the sucrose ester to the phospholipid is 1: 1-4, and the total mass fraction of the sucrose ester and the phospholipid in the ethanol solution is 1-3% to prepare a purslane emulsion with the mass fraction of 6-8%;

(d) pouring the purslane emulsion prepared in the step (c) into the wall material solution prepared in the step (b), wherein the volume ratio of the purslane emulsion to the wall material solution is 0.8-1: 1, and carrying out emulsification and homogenization treatment at 50 ℃ to prepare an initial product;

(e) and (3) carrying out spray drying on the prepared initial product, wherein the inlet temperature of the spray drying is 150-175 ℃, the outlet temperature of the spray drying is 80-90 ℃, and the feeding speed is 0.5-1.5L/min, so as to prepare the required purslane microcapsule finished product.

Preferably, the plantain seed husk dietary fiber and chia seed dietary fiber in the step (4) can be prepared by the following preparation method

(a) And (3) low-temperature superfine grinding: putting Plantago ovata seed husk or chia seed into low-temperature superfine grinding equipment for superfine grinding, and sieving with a 500-mesh sieve to obtain Plantago ovata seed husk superfine powder or chia seed superfine powder;

(b) enzymolysis: putting Plantago ovata husk superfine powder or chia seed superfine powder into purified water of 8-10 times, uniformly stirring to prepare a dispersion, heating to raise the temperature and keep the temperature at 50 +/-2 ℃, adding alpha-amylase into the dispersion, uniformly stirring for enzymolysis for 0.5-1.5 h, wherein the addition amount of the alpha-amylase is 0.4-0.6% of the content of a substrate, adjusting the pH value to 2.5-3.5, adding acidic protease and pectinase into the dispersion, uniformly stirring for enzymolysis for 2-4 h, wherein the addition amount of the acidic protease is 0.5-0.8% of the content of the substrate, the addition amount of the pectinase is 0.3-0.6% of the content of the substrate, and inactivating the enzyme by using a high-temperature instant enzyme inactivating method after the enzymolysis is finished;

(c) alcohol dissolving: adding edible ethanol accounting for 80-120% of the total weight of the enzymolysis liquid into the enzymolysis liquid, keeping the temperature at 60 +/-5 ℃, continuously stirring for 20-40 min, dissolving various fat-soluble components contained in insoluble suspended matters in the enzymolysis liquid, and performing centrifugal treatment on the enzymolysis liquid to obtain part of dietary fibers;

(d) membrane separation: concentrating the enzymolysis liquid under vacuum and reduced pressure to recover the solvent, concentrating until the relative density is 1.01-1.02, filtering by a membrane separation technology to obtain another part of dietary fiber solution with the molecular weight obviously different from other nutrients, wherein the thickness of a filter membrane is 80-150 mu m, the pore diameter of the filter membrane is 2-8 mu m, and the operating pressure is 0.12-0.25 MPa; concentrating the dietary fiber solution under vacuum and reduced pressure to obtain a concentrated solution with the relative density of 1.15-1.25;

(e) vacuum microwave drying: collecting the dietary fiber concentrated solution obtained by membrane separation and the dietary fiber obtained by centrifugation, and placing the dietary fiber concentrated solution and the dietary fiber obtained by centrifugation in a vacuum microwave drying device for drying treatment, wherein the drying condition is a microwave vacuum drying stage, the vacuum pressure is 3.0-5.5kpa, the microwave power density is 0.75-0.85W/g, and the water content is controlled to be 2-4%, so that the psyllium husk dietary fiber and chia seed dietary fiber are finally obtained.

Preferably, the preparation method of the hawthorn fruit powder in the step (5) is as follows

(a) Vacuum freeze drying: slicing hawthorn fruits, controlling the average thickness of the hawthorn fruit slices to be 2-4 mm, putting the hawthorn fruit slices into vacuum freeze drying equipment for drying, setting the parameters to be 0.4-0.6 ℃/min of pre-freezing rate, the pre-freezing initial temperature of-30-45 ℃, the pre-freezing final temperature of-70 ℃, the pressure of a drying chamber of 55-85 pa, the temperature of a heating plate of 38-45 ℃ and the drying time of 20-28 h;

(b) and (3) low-temperature superfine grinding: and (3) putting the dried hawthorn freeze-dried slices into a low-temperature ultrafine pulverizer for low-temperature ultrafine grinding, wherein 100% of the obtained hawthorn fruit powder is required to pass through a 300-mesh sieve.

In the invention, the Plantago ovata forsk shell dietary fiber and chia seed dietary fiber are obtained by carrying out low-temperature superfine grinding, membrane separation and vacuum microwave drying on Plantago ovata forsk shells and chia seeds, wherein the content of soluble dietary fiber and insoluble dietary fiber reaches more than 93%, and the Plantago ovata forsk shell dietary fiber and chia seed dietary fiber have the advantages of high purity, less impurities, good effect, high stability and greatly improved bioavailability; the hawthorn fruit powder is obtained by carrying out vacuum freeze drying and low-temperature superfine grinding on hawthorn fruits, the retention rate of various effective components such as vitamins, flavones and the like in the fruits is greatly improved, and 100% of the hawthorn fruit powder can pass through a 300-mesh sieve; the cockscomb extract and potentilla chinensis extract are obtained by carrying out alcohol reflux extraction, reduced pressure concentration, vacuum freeze drying and low-temperature superfine grinding on cockscomb and potentilla chinensis medicinal materials; the purslane microcapsule is obtained by sequentially carrying out supercritical extraction, emulsification and homogenization microcapsule embedding and spray drying on a purslane medicinal material, wherein the embedding rate of active ingredients in the purslane microcapsule is more than 90%, and the particle size is 50-260 mu m. The obtained purslane microcapsule has good solubility and good stability to light and heat. The corn oligopeptide is obtained by performing size mixing, enzymolysis, centrifugal filtration, concentration and spray drying on corn protein powder, wherein oligopeptide components with the molecular weight of less than 1000D in the corn oligopeptide account for more than 95 percent, so that the absorption and utilization rate of the corn oligopeptide by an organism is effectively improved, and the effect of absorption without digestion or with digestion slightly is achieved.

Preferably, the neutral protease is selected from bacillus subtilis neutral protease, and the enzyme activity is 20 ten thousand U/g.

The composition can be prepared into various pharmaceutically acceptable dosage forms such as hard capsules, granules, powder or tablets by a conventional preparation process so as to store and control the content of active ingredients.

Compared with the prior art, the invention has the advantages that:

the composition mainly focuses on two aspects of effects, namely the effect of reducing blood fat and the effect of improving the gastrointestinal tract function.

Aiming at the efficacy of reducing blood fat, the purslane is mainly prepared into the microcapsule to be matched with hawthorn fruit powder, red yeast rice powder and corn oligopeptide which also have the functions of reducing blood fat and regulating blood pressure, so that the functions of regulating blood fat balance and reducing the contents of cholesterol and triglyceride in blood are obviously improved. Red yeast rice is rich in natural lovastatin, and has effects of invigorating spleen, resolving food stagnation, promoting blood circulation, removing blood stasis, reducing blood lipid, and treating and preventing cardiovascular disease and cerebrovascular disease. Red rice not only can strongly reduce Total Cholesterol (TC) and low-density lipoprotein (LDL), but also can reduce Triacylglycerol (TG) to a certain extent and can also increase high-density lipoprotein (HDL). The purslane has the functions of clearing away heat and toxic materials, cooling blood and stopping bleeding, treating diarrhea and enteritis of dysentery, carbuncle sore and pyogenic infections, diminishing inflammation and easing pain, improving the health of gastrointestinal tracts, protecting liver and reducing blood fat. Omega-3 fatty acids rich in purslane can inhibit the absorption of human body to cholesterol acid, inhibit the synthesis of lipid and lipoprotein in liver, reduce the concentration of blood cholesterol, promote the discharge of cholesterol from excrement, improve the elasticity of vessel wall, expand coronary artery, reduce thrombosis, delay the process of atherosclerosis, and is very beneficial to preventing and treating cardiovascular diseases. The hawthorn flavone rich in hawthorn fruits can obviously reduce the concentration of serum Total Cholesterol (TC), low-density lipoprotein cholesterol and apolipoprotein of hyperlipaemia people, obviously increase the concentration of high-density lipoprotein cholesterol (HDL _ C) and apolipoprotein, inhibit the synthesis of liver cholesterol, and promote the intake of plasma cholesterol by liver to play a role in reducing blood fat. The corn oligopeptide can protect liver, relieve alcoholism and sober up, and has auxiliary protection effect on chemical liver injury. A large number of experiments prove that the purslane microcapsule, hawthorn fruit powder, red yeast rice powder and corn oligopeptide are compatible for use according to a specific proportion, so that the synergistic effect can be achieved, the functions of regulating blood fat balance and reducing the contents of cholesterol and triglyceride in blood can be obviously improved.

Aiming at the effect of improving the gastrointestinal function, the cockscomb extract and the potentilla chinensis extract are combined with the polydextrose, the konjac powder and the pueraria powder which are rich in dietary fibers, so that the stimulation to the gastrointestinal tract is small, and the special effects of mildness and nourishing, bowel relaxing, heat clearing and detoxifying, hemostasis and detumescence and ulcer removal, intestinal flora balance regulation and gastrointestinal function improvement can be achieved. The composition is rich in various soluble dietary fibers and insoluble dietary fibers, has low energy, is easy to generate satiety, can promote gastrointestinal peristalsis and accelerate gastrointestinal tract emptying, and simultaneously has strong water absorption of the dietary fibers, thereby playing the roles of softening stool, relaxing bowel and preventing constipation. The traditional Chinese medicines of the cockscomb and the potentilla chinensis in the formula are mainly used for clearing away heat and toxic materials, stopping bleeding, reducing swelling and removing ulcer, and a large number of experiments prove that the cockscomb and the potentilla chinensis are matched according to the formula proportion of the composition disclosed by the invention, so that the synergistic effect is achieved, and the effects of relaxing bowel, clearing away heat and toxic materials, stopping bleeding, reducing swelling and removing ulcer and regulating intestinal flora balance can be greatly improved.

The blood fat reducing composition comprises purslane microcapsules, hawthorn fruit powder, red yeast rice powder and corn oligopeptide, and has good functions of reducing blood fat and reducing cholesterol, and meanwhile, a large amount of dietary fibers in the hawthorn fruit powder and the red yeast rice powder cannot be directly absorbed by a human body, but can be effectively utilized by intestinal beneficial bacteria groups, so that colonization and multiplication of intestinal beneficial bacteria are promoted, intestinal microecological balance is improved, and intestinal peristalsis is stimulated, so that the effects of enhancing the bowel relaxing and gastrointestinal functions of the composition are achieved.

The composition for regulating the balance of intestinal flora comprises cockscomb extract, potentilla chinensis extract, polydextrose, konjac powder and radix puerariae powder, wherein rich dietary fibers are interwoven and wrapped with carbohydrate, fatty acid and other compounds in the intestinal tract, so that the digestion and absorption of the carbohydrate and fat by the digestive system of a human body are delayed, and the effect of reducing the blood fat of the composition is enhanced. The cockscomb extract and the potentilla chinensis extract in the formula can clear away heat and toxic materials, stop bleeding, relieve swelling and ulcer, relax bowel, regulate intestinal flora balance, improve intestinal health and promote intestinal peristalsis emptying, so that the absorption rate of fatty acid and cholesterol in the intestinal tract is indirectly reduced, and the effect of the composition for reducing blood fat is enhanced.

The dietary fiber combination comprises Plantago ovata forsk shell dietary fiber and chia seed dietary fiber which are used as prebiotics and dietary fiber, and the substances of the Plantago ovata forsk shell dietary fiber and chia seed dietary fiber can prevent cholic acid or cholesterol from being absorbed by intestinal tracts and promote the cholic acid or cholesterol to be discharged along with excrement, so that the contents of cholesterol and triglyceride in blood are indirectly reduced, the effect of reducing blood fat of the composition is enhanced, and the effects of regulating intestinal tract microecological balance, improving gastrointestinal tract functions, relaxing bowel and controlling the content of cholesterol can be achieved.

The preparation method of the invention is that purslane is processed by supercritical CO₂Extracting and embedding to prepare microcapsules, extracting the traditional Chinese medicines such as cockscomb, potentilla chinensis and the like by alcohol reflux, concentrating under reduced pressure, freeze-drying in vacuum, and crushing to prepare extracts, so that the main functional components of the medicines are retained to the maximum extent, and the drug effect is remarkably improved; the Plantago ovata forsk husk and chia seed are subjected to ultramicro crushing, enzymolysis, alcohol dissolving, membrane separation and vacuum microwave drying treatment to extract dietary fiber with the purity of more than 93 percent, so that the dosage of the composition is obviously reduced; the hawthorn fruit is subjected to vacuum freeze drying and low-temperature superfine grinding to prepare hawthorn fruit powder, so that the nutrient substances in the hawthorn fruit are retained to the greatest extent; corn oligopeptide is prepared by mixing, carrying out enzymolysis, carrying out centrifugal filtration, carrying out reduced pressure concentration and spray drying on corn protein powder, wherein the oligopeptide component with the molecular weight of less than 1000D accounts for more than 95 percent, so that the absorption and utilization rate of the corn oligopeptide by an organism is effectively improved, and the effect of absorption without digestion or with digestion slightly is achieved; through the treatment of the preparation process, the edible amount of the composition is obviously reduced, the efficacy and the nutrient components are enriched, the efficacy is obviously improved, and the bioavailability of the raw materials is improved.

Drawings

FIG. 1 shows the data results of MTT experiments in the examples of the present invention;

FIG. 2 shows the intracellular TG results of the experiment on the metabolism of Hep G2 glycolipid in the example of the present invention;

FIG. 3 is the supernatant TG results of Hep G2 glycolipid metabolism experiments in the examples of the present invention;

FIG. 4 is a fluorescent phenotype plot of zebra fish enterokinesia in an embodiment of the invention;

FIG. 5 is a graph showing the total fluorescence intensity of the intestinal tracts of zebrafish in the present invention compared to that of the normal control group, where p is < 0.001;

fig. 6 shows the effect of the compositions of the present invention on the promotion of intestinal peristalsis in zebrafish <0.001 compared to normal controls.

Detailed Description

The present invention will be described in further detail with reference to examples.

The formula of the composition for reducing blood fat and improving gastrointestinal function according to the present invention is shown in examples 1 to 8 in table 1.