阅读说明：本技术 一种木糖酸催化生产低聚木糖的方法 (Method for producing xylooligosaccharide by catalyzing xylonic acid ) 是由 徐勇 周鑫 于 2019-10-28 设计创作，主要内容包括：本发明公开了一种木糖酸催化生产低聚木糖的方法,其中,一种木糖酸生产低聚木糖的方法,其包括,将木聚糖原料和木糖酸混合,加热搅拌；按质量份数计,所述木糖原料为1份,所述木糖酸为5～12份；一种发酵催化生产低聚木糖的方法,其包括,将木糖与菌体混合,调节pH,低温搅拌；加入木聚糖原料,加热搅拌。本发明利用微生物全细胞生物氧化木糖至木糖酸的反应,将产生的木糖酸作为催化剂,与醋酸和其他无机酸相比,产生的聚糖不易过度降解,得率高且副产品木糖糠醛少。(The invention discloses a method for producing xylo-oligosaccharide by using xylonic acid as a catalyst, wherein the method for producing xylo-oligosaccharide by using xylonic acid comprises the steps of mixing a xylan raw material with xylonic acid, heating and stirring; according to the mass parts, the xylose raw material accounts for 1 part, and the xylonic acid accounts for 5-12 parts; a method for producing xylo-oligosaccharide by fermentation and catalysis comprises mixing xylose with thallus, adjusting pH, and stirring at low temperature; adding xylan material, heating and stirring. The invention utilizes the reaction of microbial whole-cell biological oxidation xylose to xylonic acid, and the generated xylonic acid is used as a catalyst, compared with acetic acid and other inorganic acids, the produced glycan is not easy to excessively degrade, the yield is high, and the byproduct xylose furfural is less.)

1. A method for producing xylooligosaccharide by xylonic acid is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

mixing xylan raw material and xylonic acid, heating and stirring;

the xylose raw material accounts for 1 part by weight, and the xylonic acid accounts for 5-12 parts by weight.

2. A process for the production of xylo-oligosaccharides as claimed in claim 1, characterized in that: the xylan raw material is xylan and/or a wood fiber raw material containing xylan.

3. A process for the production of xylo-oligosaccharides as claimed in claim 1, characterized in that: the heating and stirring reaction is carried out, wherein the stirring speed is 30-100 rmp, the temperature is 130-170 ℃, and the time is 0.25-2.0 h.

4. A method of producing xylo-oligosaccharides as claimed in any one of claims 1 to 3, characterized in that: also comprises the following steps of (1) preparing,

adjusting pH after cooling, adding thalli, and stirring at low temperature;

adding xylan material, heating and stirring.

5. The method of catalytically producing xylo-oligosaccharides of claim 4, characterized in that: the thalli is xylose oxidizing bacillus, and the weight is 0.01-0.1 part.

6. The method of catalytically producing xylo-oligosaccharides of claim 4, characterized in that: and after the temperature is reduced, adjusting the pH value to be reduced to room temperature and then adjusting the pH value to be weakly acidic, stirring at a low temperature of 25-35 ℃ and at a stirring speed of 100-200 rmp.

7. The method of catalytically producing xylo-oligosaccharides of claim 4, characterized in that: the mixing of the xylan raw material and the xylonic acid is different from the xylan raw material added to the xylan raw material.

8. A method for producing xylooligosaccharide by fermentation catalysis is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

mixing xylose with the thallus, adjusting the pH value, and stirring at low temperature;

adding xylan material, heating and stirring.

9. The method for fermentative catalytic production of xylo-oligosaccharide according to claim 8, wherein: the thalli is xylose oxidizing bacillus, and the xylan raw material xylan and/or a wood fiber raw material containing xylan;

according to the mass parts, the xylose accounts for 1 part, the thalli accounts for 0.01-0.1 part, and the xylan raw material accounts for 1-5 parts.

10. The process for the fermentative catalytic production of xylo-oligosaccharides according to claim 8 or 9, characterized in that: and adjusting the pH value of the xylose solution to be weakly acidic, stirring at a low temperature of 25-35 ℃ at a stirring speed of 100-200 rmp, heating and stirring for reaction at a stirring speed of 30-100 rmp at a temperature of 130-170 ℃ for 0.25-2.0 h.

Technical Field

The invention belongs to the technical field of food engineering and chemical engineering, and particularly relates to a method for producing xylooligosaccharide by catalyzing xylonic acid.

Background

Xylo-oligosaccharide is also called xylo-oligosaccharide which is mainly derived from hydrolysis of xylan in wood fiber, is used as a functional food or feed additive, cannot be absorbed by a digestive system, but can selectively proliferate bifidobacteria in intestinal tract and simultaneously activate multiple immune cell activities; therefore, under the traction and driving of the high-speed development of the industries such as human big health, micro-ecology, food safety, green animal breeding, ecological agriculture and the like, the xylo-oligosaccharide product derived from the wood fiber has very wide development prospect as a super-strong prebiotic. When the current xylo-oligosaccharide production is mainly carried out by catalyzing and hydrolyzing alkaline-extracted xylan by using an endo-xylanase preparation, the method depends on high-price biological enzyme, the cost is high, the period is long, and the alkaline extraction treatment process is complex and difficult; in addition, the common strong acid hydrolysis method can be adopted to prepare the xylo-oligosaccharide, the yield of the xylo-oligosaccharide is low, the byproducts are more, and the product quality is not high.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above-mentioned technical drawbacks.

Therefore, as one aspect of the present invention, the present invention overcomes the deficiencies of the prior art and provides a method for producing xylooligosaccharide by xylonic acid catalysis.

In order to solve the technical problems, the invention provides the following technical scheme: a method for producing xylooligosaccharide with xylonic acid comprises mixing xylan raw material and xylonic acid, heating and stirring; the xylose raw material accounts for 1 part by weight, and the xylonic acid accounts for 5-12 parts by weight.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: the xylan raw material is xylan and/or a wood fiber raw material containing xylan.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: the heating and stirring reaction is carried out, wherein the stirring speed is 30-100 rmp, the temperature is 130-170 ℃, and the time is 0.25-2.0 h.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: cooling, adjusting the pH value, adding thalli, and stirring at low temperature; adding xylan material, heating and stirring.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: the thalli is xylose oxidizing bacillus, and the weight is 0.01-0.1 part.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: and after the temperature is reduced, adjusting the pH value to be reduced to room temperature and then adjusting the pH value to be weakly acidic, stirring at a low temperature of 25-35 ℃ and at a stirring speed of 100-200 rmp.

The preferable scheme of the method for producing xylooligosaccharide by using xylonic acid provided by the invention is as follows: the mixing of the xylan raw material and the xylonic acid is different from the xylan raw material added to the xylan raw material.

As another aspect of the invention, the invention overcomes the deficiency existing in the prior art, provide a method for producing xylo-oligosaccharide by fermentation catalysis, it includes, mix xylose with thalli, regulate pH, stir at low temperature; adding xylan material, heating and stirring.

As a preferable scheme of the method for producing xylooligosaccharide by fermentation catalysis, the method comprises the following steps: the thalli is xylose oxidizing bacillus, and the xylan raw material xylan and/or a wood fiber raw material containing xylan; according to the mass parts, the xylose accounts for 1 part, the thalli accounts for 0.01-0.1 part, and the xylan raw material accounts for 1-5 parts.

As a preferable scheme of the method for producing xylooligosaccharide by fermentation catalysis, the method comprises the following steps: and adjusting the pH value of the xylose solution to be weakly acidic, stirring at a low temperature of 25-35 ℃ at a stirring speed of 100-200 rmp, heating and stirring for reaction at a stirring speed of 30-100 rmp at a temperature of 130-170 ℃ for 0.25-2.0 h.

The invention has the beneficial effects that:

the method adopts the xylonic acid as the catalyst, so that the yield is high, and the byproduct xylose furfural is less; the invention selects biological oxidation and electrodialysis coupling technology to convert xylose into xylonic acid, wherein the xylonic acid can be recovered as self-supply catalyst and xylo-oligosaccharide product is purer.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:

FIG. 1 is a flow chart of the production process in example 6.

FIG. 2 is a schematic diagram of the electrodialysis reaction in example 6.

FIG. 3 is a high performance anion exchange chromatography profile of example 1.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.