阅读说明：本技术 一种缓解热应激损伤肉鸡心肌的中药提取物及其制备方法 (Traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle and preparation method thereof ) 是由 陈洪博 段滇宁 王�华 周孝琼 杨润泽 洪麒翔 于 2019-10-31 设计创作，主要内容包括：本发明公开了一种缓解热应激损伤肉鸡心肌的中药提取物及其制备方法,所述中药提取物由以下重量份的原料制备而成：丹参10-30份,银杏叶10-30份,葛根10-30份,绞股蓝10-20份,酸枣仁5-20份,山楂5-20份,甘草5-20份。制备方法如下：1)将丹参,银杏叶、葛根,绞股蓝、酸枣仁、山楂、甘草洗净晾干,粉碎后过筛,得到混合粉末；2)对粉末进行浸提、过滤得浸提液；3)对浸提液进行粗过滤和抽滤得滤液；4)对滤液蒸馏、浓缩得到中药提取物。将所述中药提取物和饮用水按照2.5-7.5 g∶1L的比例混合均匀后用于投喂肉鸡,能够有效缓解肉鸡心脏的热应激损伤问题,保证鸡群生命健康。(The invention discloses a traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle and a preparation method thereof, wherein the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 10-30 parts of salvia miltiorrhiza, 10-30 parts of ginkgo leaves, 10-30 parts of kudzu roots, 10-20 parts of gynostemma pentaphylla, 5-20 parts of spina date seeds, 5-20 parts of hawthorn and 5-20 parts of liquorice. The preparation method comprises the following steps: 1) cleaning Saviae Miltiorrhizae radix, folium Ginkgo, radix Puerariae, herba Gynostemmatis, semen Ziziphi Spinosae, fructus crataegi, and Glycyrrhrizae radix, air drying, pulverizing, and sieving to obtain mixed powder; 2) leaching and filtering the powder to obtain a leaching solution; 3) carrying out coarse filtration and suction filtration on the leaching liquor to obtain filtrate; 4) distilling the filtrate, and concentrating to obtain Chinese medicinal extract. The traditional Chinese medicine extract and drinking water are uniformly mixed according to the proportion of 2.5-7.5 g: 1L and then are used for feeding broiler chickens, so that the problem of heat stress injury of the hearts of the broiler chickens can be effectively relieved, and the life health of the chicken groups is ensured.)

1. A traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle is characterized in that; the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 10-30 parts of salvia miltiorrhiza, 10-30 parts of ginkgo leaves, 10-30 parts of kudzu roots, 10-20 parts of gynostemma pentaphylla, 5-20 parts of spina date seeds, 5-20 parts of hawthorn and 5-20 parts of liquorice.

2. The traditional Chinese medicine extract for relieving heat stress injury of broiler chicken myocardium according to claim 1, is characterized in that; the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 20 parts of salvia miltiorrhiza, 20 parts of ginkgo leaves, 15 parts of kudzuvine roots, 15 parts of gynostemma pentaphylla, 10 parts of spina date seeds, 10 parts of hawthorn and 10 parts of liquorice.

3. The preparation method of the traditional Chinese medicine extract for relieving heat stress injury of broiler chicken myocardium as claimed in claim 1, characterized in that: which comprises the following steps:

1) mixing the raw materials in parts by weight, cleaning and airing the salvia miltiorrhiza, the ginkgo leaves, the kudzuvine roots, the gynostemma pentaphylla, the spina date seeds, the hawthorns and the liquorice with clear water, crushing, and sieving with a sieve of 80-100 meshes to obtain mixed powder;

2) extracting the mixed powder with ethanol, filtering, and collecting primary filtrate and filter residue;

3) extracting the filter residue with ethanol, filtering, and collecting the secondary filtrate;

4) mixing the primary filtrate and the secondary filtrate, and concentrating to obtain a concentrated solution;

5) and (4) freeze-drying the concentrated solution to obtain the traditional Chinese medicine extract.

4. The preparation method of the traditional Chinese medicine extract for relieving heat stress injury of broiler chicken myocardium according to claim 3, is characterized in that: the step 2) is as follows: soaking the mixed powder in ethanol for more than 48h according to the volume ratio of the weight of the mixed powder to the ethanol of 1 g: 10-12 mL, then ultrasonically extracting for 30-40min by using ultrasonic waves with the temperature of 45-50 ℃ and the power of 75-85%, carrying out suction filtration by using a Buchner funnel vacuum pump, and collecting primary filtrate and filter residue.

5. The preparation method of the traditional Chinese medicine extract for relieving heat stress injury of broiler chicken myocardium according to claim 3, is characterized in that: the step 3) is as follows: soaking the mixed powder in ethanol for more than 48h according to the volume ratio of the weight of the filter residue to the ethanol of 1 g: 7-10 mL, then ultrasonically extracting for 30-40min by using ultrasonic waves with the temperature of 45-50 ℃ and the power of 75-85%, performing suction filtration by using a Buchner funnel vacuum pump, and collecting secondary filtrate.

6. The use of the extract of a Chinese traditional medicine obtained by the preparation method according to any one of claims 3 to 5 is characterized in that: the Chinese medicinal extract is dissolved in water and then used for feeding broiler chickens.

7. The use of the herbal extract of claim 6, wherein: the mixing ratio of the traditional Chinese medicine extract to water is 2.5-7.5 g: 1L.

8. The use of the herbal extract of claim 7, wherein: the mixing ratio of the traditional Chinese medicine extract to water is 5.0 g: 1L.

Technical Field

The invention belongs to the technical field of animal breeding, and particularly relates to a traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle and a preparation method thereof.

Background

With the increase of extreme weather in the world, the global warming caused by the greenhouse effect, the role of heat stress is more and more important. In recent years, reports on heat stress loss have been on an increasing trend. The heat stress stimulation not only can obviously influence the growth, development, immunocompetence and productivity of the broiler chickens, but also can cause sudden death of the broiler chickens. From the present research data, the main cause of sudden death of animals during stress is that the tissue cells of important vital organs (heart) of animals suffer severe stress pathological damage. At present, the heat stress reaction, stress injury and stress related diseases in the domestic veterinary field still lack necessary research, and the basic research work is very weak. Therefore, the method has important significance for reducing the direct and indirect loss caused by heat stress to the broiler breeding industry.

The development of the breeding industry is developed towards the directions of high efficiency, intensive, high density, green and the like, the breeding environment and the feeding additives can become a part of important factors influencing the yield and the quality of the broilers, and the phenomenon that the broilers are subjected to heat stress due to the problems of hot high temperature, long duration and high-density breeding in summer in global warming, especially in most places in the south of China, is more common. The occurrence of heat stress causes huge losses to the breeding companies or farmers. At present, some medicines can relieve heat stress, such as electrolytic multivitamin, vitamin C and the like, but the actual effects of vitamin C, electrolytic multivitamin and the like are not satisfactory due to the wide damage of the heat stress to the body. The active substances of the traditional Chinese medicine extract have the following effects: regulating acid-base balance of the organism, thereby relieving respiratory alkalosis of the animal organism under heat stress condition; the normal metabolism of the organism in the new castle is ensured by regulating the cortisol content of the plasma of the organism and enhancing the immunologic function and the anti-stress capability of the organism; regulating blood circulation and improving blood viscosity; tranquilizing and allaying excitement, etc. The Chinese herbal medicine has wide sources and low residue, and is a product of nature, so the Chinese herbal medicine has wide application prospect in the aspect of resisting heat stress injury.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide a traditional Chinese medicine extract for relieving heat stress injury of broiler myocardium and a preparation method thereof.

In order to achieve the purpose, the technical scheme of the invention is as follows:

a traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle is prepared from the following raw materials in parts by weight: 10-30 parts of salvia miltiorrhiza, 10-30 parts of ginkgo leaves, 10-30 parts of kudzu roots, 10-20 parts of gynostemma pentaphylla, 5-20 parts of spina date seeds, 5-20 parts of hawthorn and 5-20 parts of liquorice.

Preferably, the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 20 parts of salvia miltiorrhiza, 20 parts of ginkgo leaves, 15 parts of kudzuvine roots, 15 parts of gynostemma pentaphylla, 10 parts of spina date seeds, 10 parts of hawthorn and 10 parts of liquorice.

The preparation method of the traditional Chinese medicine extract for relieving heat stress injury of broiler chicken cardiac muscle comprises the following steps:

1) mixing the raw materials in parts by weight, cleaning and airing the salvia miltiorrhiza, the ginkgo leaves, the kudzuvine roots, the gynostemma pentaphylla, the spina date seeds, the hawthorns and the liquorice with clear water, crushing, and sieving with a sieve of 80-100 meshes to obtain mixed powder;

2) extracting the mixed powder with ethanol, filtering, and collecting primary filtrate and filter residue;

3) extracting the filter residue with ethanol, filtering, and collecting the secondary filtrate;

4) mixing the primary filtrate and the secondary filtrate, and concentrating to obtain a concentrated solution;

5) and (3) freeze-drying the concentrated solution by using a vacuum freeze-drying machine to obtain the traditional Chinese medicine extract.

Further, the step 2) is specifically as follows: soaking the mixed powder in ethanol for more than 48h according to the volume ratio of the weight of the mixed powder to the ethanol of 1 g: 10-12 mL, then ultrasonically extracting for 30-40min by using ultrasonic waves with the temperature of 45-50 ℃ and the power of 75-85%, carrying out suction filtration by using a Buchner funnel vacuum pump, and collecting primary filtrate and filter residue.

Further, the step 3) is specifically as follows: soaking the mixed powder in ethanol for more than 48h according to the volume ratio of the weight of the filter residue to the ethanol of 1 g: 7-10 mL, then ultrasonically extracting for 30-40min by using ultrasonic waves with the temperature of 45-50 ℃ and the power of 75-85%, performing suction filtration by using a Buchner funnel vacuum pump, and collecting secondary filtrate.

The traditional Chinese medicine extract is dissolved in water according to the mixing ratio of the traditional Chinese medicine extract to the water of 2.5-7.5 g: 1L and then is used for feeding the broilers, so that the problem of heat stress injury of the hearts of the broilers can be effectively relieved.

The formula of the invention is as follows: when the broiler chicken generates heat stress, the sympathetic-adrenal medullary system is excited to cause the acceleration of heart rate, the enhancement of heart contractility, the increase of coronary artery blood flow, the increase of peripheral resistance and the increase of myocardial damage; in order to keep heat balance, broiler chickens can increase respiratory rate and lung ventilation to increase evaporation and convection heat dissipation of respiratory tract, so that respiration is accelerated, and CO in vivo is caused₂Excessive loss can reduce the content of bicarbonate in blood, cause respiratory alkalosis to increase the pH value in blood, damage blood circulation tissues and organs, even cause sudden death of broiler chickens, and seriously restrict the development of broiler chicken industry. Therefore, some traditional Chinese medicines for clearing heat and cooling blood, relieving cough and asthma, promoting the production of body fluid to quench thirst, reducing blood pressure, soothing the nerves, clearing heat and removing toxicity and the like should be selected in the aspect of preventing and controlling heat stress. In the formula, the salvia miltiorrhiza has the effects of activating blood and removing stasis, stimulating menstrual flow and relieving pain, clearing away heart-fire and relieving restlessness, cooling blood and eliminating carbuncle; folium Ginkgo has effects of promoting blood circulation, removing blood stasis, dredging collaterals, relieving pain, astringing lung, relieving asthma, eliminating turbid pathogen, and reducing blood lipid; radix Puerariae has effects of relieving muscles and fever, promoting eruption, promoting fluid production to quench thirst, invigorating yang and relieving diarrhea; the gynostemma pentaphylla has the effects of reducing blood fat, regulating blood pressure, preventing and treating thrombus, preventing and treating cardiovascular diseases, regulating blood sugar, promoting sleep, delaying senility, preventing and resisting cancer, improving immunity and regulating physiological functions of a human body; the spina date seed has the effects of nourishing heart and liver, calming heart and tranquilizing mind, arresting sweating and promoting fluid production; the fructus crataegi has effects of reducing blood lipid, lowering blood pressure, tonifying heart, and resisting arrhythmia; the licorice has the effects of clearing away heat and toxic materials, eliminating phlegm, relieving cough, invigorating spleen, replenishing qi and strengthening spleen and stomach. The formula of the invention is based on a large amount of clinical application and is combined with modern epidemic diseasesThe feed additive is prepared by theoretical screening of nutrition feed science, is used for protecting the heart muscle of the broiler chicken from heat stress injury, and has good effect.

The traditional Chinese medicine extract prepared by the invention mainly contains flavonoid, lactone and glycoside substances, and is used for feeding broiler chickens after being uniformly mixed according to the proportion of 2.5-7.5 g: 1L of the traditional Chinese medicine extract and drinking water, so that the problem of heat stress injury of hearts of the broiler chickens can be effectively relieved, and the life health of chicken groups is ensured.

Drawings

FIG. 1 shows the ion content in the serum of each treatment group in the application example;

FIG. 2 shows the biochemical index content of blood in each treatment group in the application example;

fig. 3 is the pathological observation results of the myocardial sections of each treatment group in the application example, a: a control group; b: a heat stress group; c: electrolyzing the multidimensional group; d: a low dose group; e: a medium dose group; f: high dose group.

Detailed Description

In the description of the present invention, those whose specific conditions are not specified in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.