阅读说明：本技术 一种三相溶剂浸提法提取藻油的制备方法 (Preparation method for extracting algae oil by three-phase solvent extraction method ) 是由 邓敏丽 于 2018-06-23 设计创作，主要内容包括：本发明提供了生物柴油技术邻域内的一种三相溶剂浸提法提取藻油的制备方法,其先定量称量藻粉,定量量取氯仿、甲醇、水三种溶剂,然后将氯仿、甲醇、水三种溶剂按一定比例混和均匀,加入藻粉中,静置一定时间后离心,收集上清液,下层沉淀继续离心收集上清液,重复三次,合并上清液,最后上清液中按比例加入一定量的氯仿和水,出现明显分层,静置,然后将下层氯仿收集到烧杯中,恒温水浴蒸发溶剂,称重,计算油脂得率；本发明制备方法简单,开发成本低、生产效率高且不受不可持续性的限制。(The invention provides a preparation method for extracting algae oil by a three-phase solvent extraction method in the technical neighborhood of biodiesel, which comprises the steps of weighing algae powder in a fixed amount, weighing three solvents of chloroform, methanol and water in a fixed amount, then uniformly mixing the three solvents of chloroform, methanol and water according to a certain proportion, adding the mixture into the algae powder, standing for a certain time, centrifuging, collecting supernatant, continuously centrifuging the lower-layer precipitate, collecting supernatant, repeating the steps for three times, combining the supernatant, finally adding a certain amount of chloroform and water into the supernatant according to a certain proportion, obviously layering, standing, collecting the lower-layer chloroform into a beaker, evaporating the solvent by constant-temperature water bath, weighing, and calculating the yield of oil; the preparation method is simple, low in development cost, high in production efficiency and not limited by unsustainability.)

1. A method for extracting algae oil by a three-phase solvent extraction method is characterized by comprising the following steps:

(A1) weighing: quantitatively weighing algae powder, and quantitatively measuring three solvents of chloroform, methanol and water;

(A2) adding a solvent: mixing chloroform, methanol and water at a certain ratio, adding into algae powder, standing for a certain time, centrifuging, collecting supernatant, centrifuging the lower layer precipitate, collecting supernatant, repeating for three times, and mixing the supernatants;

(A3) extraction: adding a certain amount of chloroform and water into the supernatant according to a certain proportion, obviously layering, standing, collecting the lower layer of chloroform into a beaker, evaporating the solvent in a constant-temperature water bath, weighing, and calculating the yield of the oil.

2. The method for preparing algal oil by three-phase solvent extraction according to claim 1, wherein in the step (A2), the chloroform: methanol: the proportion of water is 1: 1: 0.1-2: 2: 1.

3. the method for preparing algal oil by three-phase solvent extraction according to claim 1, wherein in the step (A3), the chloroform: the proportion of water is 1: 2-2: 1.

Background

Biodiesel is a sustainable, non-toxic and biodegradable diesel fuel substitute due to the advantages of environmental protection, lubricity and the like, can meet energy requirements, can be developed and produced continuously through various biomasses, and gradually becomes the key point of new energy research and development.

Disclosure of Invention

Aiming at overcoming the defects in the prior art, the invention provides the preparation method for extracting the algae oil by the three-phase solvent extraction method, which has the advantages of simple preparation method, low development cost, high production efficiency and no restriction of unsustainability.

The purpose of the invention is realized by the following technical scheme: a preparation method for extracting algae oil by a three-phase solvent extraction method comprises the following steps:

(A1) weighing: quantitatively weighing algae powder, and quantitatively measuring three solvents of chloroform, methanol and water;

(A2) adding a solvent: mixing chloroform, methanol and water at a certain ratio, adding into algae powder, standing for a certain time, centrifuging, collecting supernatant, centrifuging the lower layer precipitate, collecting supernatant, repeating for three times, and mixing the supernatants;

(A3) extraction: adding a certain amount of chloroform and water into the supernatant according to a certain proportion, obviously layering, standing, collecting the lower layer of chloroform into a beaker, evaporating the solvent in a constant-temperature water bath, weighing, and calculating the yield of the oil.

Firstly weighing algae powder in a fixed amount, weighing three solvents of chloroform, methanol and water in a fixed amount, then uniformly mixing the three solvents of chloroform, methanol and water according to a certain proportion, adding the mixture into the algae powder, standing for a certain time, centrifuging, collecting supernatant, continuously centrifuging the lower-layer precipitate, collecting supernatant, repeating the steps for three times, combining the supernatant, finally adding a certain amount of chloroform and water into the supernatant according to a certain proportion, obviously layering, standing, then collecting the lower-layer chloroform into a beaker, evaporating the solvent in a constant-temperature water bath, weighing, and calculating the yield of the oil; compared with the prior art, the invention has the beneficial effects that: the method has the advantages of simple preparation method, low development cost, high production efficiency and no restriction of unsustainability.

As a refinement of the present invention, the chloroform: methanol: the proportion of water is 1: 1: 0.1-2: 2: 1.

as a refinement of the present invention, the chloroform: the proportion of water is 1: 2-2: 1.

Detailed Description