阅读说明：本技术 光转换的多肽及其用途 (Photoconverted polypeptides and uses thereof ) 是由 A·斯克拉 A·赖歇特 M·道内 于 2018-05-09 设计创作，主要内容包括：本发明涉及光转换的多肽。具体而言,本发明涉及一种包含光响应元件的多肽,其中可以通过用特定波长的光照射该多肽而将该光响应元件的构型(即构型状态)在反式和顺式异构体之间转换,并且其中所述构型的转换改变了所述多肽的构象和与配体(例如目标分子)的结合活性。而且,本发明包括使用所述光转换多肽来分离和/或纯化目标分子。本发明还提供了包含本发明的光转换多肽的亲和基质、亲和色谱柱和亲和色谱设备。(The present invention relates to light-converting polypeptides. In particular, the present invention relates to a polypeptide comprising a light-responsive element, wherein the configuration (i.e. conformational state) of the light-responsive element can be switched between trans and cis isomers by irradiating the polypeptide with light of a specific wavelength, and wherein said switching of configuration changes the conformation of said polypeptide and the binding activity to a ligand (e.g. a target molecule). Furthermore, the present invention includes the use of the photoconversion polypeptide for isolating and/or purifying a target molecule. The invention also provides affinity matrices, affinity chromatography columns and affinity chromatography devices comprising the light-converting polypeptides of the invention.)