阅读说明：本技术 一种大肠杆菌快速测试盒 (Quick test box of escherichia coli ) 是由 不公告发明人 于 2019-10-15 设计创作，主要内容包括：本发明属于微生物检测技术领域,具体的说是一种大肠杆菌快速测试盒；包括盒体和与其铰接的盒盖,所述盒体内部通过两个隔板将其盒体分成加热室、接种室和反应室,且通过顶板将其密封,接种室位于加热室与反应室中间；所述顶板上贯穿设有固定筒,所述固定筒上插接有拉动杆；所述内部设置有接种装置,接种装置包括山字筒,山字筒中部与固定筒连通；本发明通过将拉动杆设置在固定筒内部,并在拉动杆上开设融化槽,培养基置于融化槽内部后,移动拉动杆,融化槽被其两侧的拉动杆封闭在固定筒内部,然后通过接种装置使不与外界空气接触的试剂接种到培养基上,培养基和试剂均不会被空气中的细菌影响,提高了检测的精度。(The invention belongs to the technical field of microbial detection, and particularly relates to a rapid test box for escherichia coli; the box body is divided into a heating chamber, an inoculation chamber and a reaction chamber by two clapboards and is sealed by a top plate, and the inoculation chamber is positioned between the heating chamber and the reaction chamber; a fixed cylinder penetrates through the top plate, and a pulling rod is inserted into the fixed cylinder; the inoculation device is arranged in the fixed cylinder and comprises a herringbone cylinder, and the middle part of the herringbone cylinder is communicated with the fixed cylinder; according to the invention, the pulling rod is arranged in the fixed cylinder, the melting tank is arranged on the pulling rod, the pulling rod is moved after the culture medium is arranged in the melting tank, the melting tank is sealed in the fixed cylinder by the pulling rods on the two sides of the melting tank, and then the reagent which is not contacted with the outside air is inoculated to the culture medium through the inoculation device, so that the culture medium and the reagent are not influenced by bacteria in the air, and the detection precision is improved.)

1. The utility model provides a quick test box of escherichia coli, includes box body (1) and rather than articulated lid (2), its characterized in that: the box body (1) is divided into a heating chamber (3), an inoculation chamber (4) and a reaction chamber (5) by two clapboards inside the box body (1), and is sealed by a top plate (6), and the inoculation chamber (4) is positioned between the heating chamber (3) and the reaction chamber (5); a fixed cylinder (7) penetrates through the top plate (6), and a pulling rod (18) is inserted into the fixed cylinder (7); the inoculation device (8) is arranged in the inoculation device (8) and comprises a Chinese character 'shan' barrel (81), the middle of the Chinese character 'shan' barrel (81) is communicated with the fixed barrel (7), an extrusion rod (82) is arranged in the inoculation device, the extrusion rod (82) is contacted with the bottom end of the pulling rod (18), an extrusion spring (83) is fixedly connected to the bottom of the extrusion rod (82), push rods (84) are arranged in the Chinese character 'shan' barrels (81) on the front side and the rear side of the extrusion rod (82), the Chinese character 'shan' barrel (81) on the top of each push rod (84) is partially bent and communicated with the top of the fixed barrel (7), and an inlet (85) is formed in the position where the Chinese character 'shan' barrel; inoculation room (4) front side be provided with check valve (9) on box body (1) wall thickness, melt groove (10) have been seted up to the part that lies in heating chamber (3) top on pull rod (18), melt groove (10) and upwards extend to solid fixed cylinder (7) surface, melt groove (10) bottom pull rod (18) surface is provided with push spring (11), and push spring (11) bottom has linked firmly application of force pole (12), and the equal both sides of application of force pole (12) set up to the scarf, application of force pole (12) bottom and solid fixed cylinder (7) inner wall contact.

2. The rapid test kit for Escherichia coli according to claim 1, wherein: a rotating shaft (13) is arranged in the melting tank (10), and a stirring plate (14) is arranged on the surface of the rotating shaft (13).

3. The rapid test kit for Escherichia coli according to claim 2, wherein: draw even board (14) surface and seted up circular slot (15) near the position department of pivot (13) to circular slot (15) are for dispersing the center, have seted up a plurality of guide ways (16) to the one end of drawing even board (14) and keeping away from pivot (13).

4. The rapid test kit for Escherichia coli according to claim 3, wherein: the distance between two adjacent guide grooves (16) at one end of the edge of the uniform stirring plate (14) is smaller when the guide grooves are closer to the circular grooves (15).

5. The rapid test kit for Escherichia coli according to claim 1, wherein: an observation window (17) is embedded in the surface of the fixed cylinder (7) at the top of the reaction chamber (5).

6. The rapid test kit for Escherichia coli according to claim 1, wherein: the front side and the rear side of the force application rod (12) are arc-shaped surfaces, and the top ends of the inclined cutting surfaces at the two sides of the force application rod (12) are lower than the top end of the force application rod (12).

Technical Field

The invention belongs to the technical field of microbial detection, and particularly relates to a rapid test box for escherichia coli.

Background

Escherichia coli, also called Escherichia coli, is a conditional pathogenic bacterium, can cause gastrointestinal tract infection or urinary tract infection of various local tissues and organs of human and various animals under certain conditions, is a normal colonizing bacterium in animal intestinal tracts, and causes diseases in a small part of the intestinal tract infection under certain conditions. The serotype of the escherichia coli can cause gastrointestinal tract infection of human bodies or animals, is mainly caused by infection of specific pilus antigens, pathogenic toxins and the like, can cause urinary tract infection, arthritis, meningitis, sepsis type infection and the like besides gastrointestinal tract infection, is a frequently living bacterium in intestinal tracts of human beings or animals, is widely stored in environments where human beings rely on survival such as food, drinking water and the like, is an important work content of food hygiene and epidemic prevention for detection of the escherichia coli and other harmful microorganisms in food, and is also one of important indexes of self-inspection of manufacturers for producing food.

When escherichia coli is detected, a detection sample needs to be placed on a culture medium, a detection solution is dripped to enable the detection sample after culture to generate a color reaction, whether escherichia coli exists or not is observed, after the culture medium is prepared, the culture medium needs to be placed near an alcohol lamp for inoculation, the culture medium is prevented from being polluted by bacteria in the air, and particularly when outdoor detection is performed, when wind is generated and the bacteria in the air reach the alcohol lamp, the alcohol lamp is subjected to the action of the wind, so that the temperature of the air is lower, and the sterilization effect is small.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a rapid test box for escherichia coli, which is mainly characterized in that a pulling rod is arranged in a fixed cylinder, a melting tank is arranged on the pulling rod, a culture medium is arranged in the melting tank, then the pulling rod is moved, the melting tank is sealed in the fixed cylinder by the pulling rods on the two sides of the melting tank, then a reagent which is not contacted with the outside air is inoculated on the culture medium through an inoculation device, and the culture medium and the reagent are not influenced by bacteria in the air, so that the problems in the background art are solved.

In order to achieve the purpose, the invention provides the following technical scheme: a quick test box for escherichia coli comprises a box body and a box cover hinged with the box body, wherein the box body is divided into a heating chamber, an inoculation chamber and a reaction chamber by two clapboards and is sealed by a top plate, and the inoculation chamber is positioned between the heating chamber and the reaction chamber; a fixed cylinder penetrates through the top plate, and a pulling rod is inserted into the fixed cylinder; the inoculating device is arranged in the inoculating device and comprises a Chinese character 'shan' barrel, the middle of the Chinese character 'shan' barrel is communicated with the fixed barrel, an extrusion rod is arranged in the inoculating device, the extrusion rod is contacted with the bottom end of the pulling rod, an extrusion spring is fixedly connected to the bottom of the extrusion rod, pushing rods are arranged in the Chinese character 'shan' barrels on the front side and the rear side of the extrusion rod, the Chinese character 'shan' barrel at the top of the pushing rods is arranged in a bending mode and communicated with the top of the fixed barrel, and an inlet opening is formed in the position; the wall thickness of the box body on the front side of the inoculation chamber is provided with a one-way valve, the part of the pull rod, which is positioned at the top of the heating chamber, is provided with a melting groove, the melting groove extends upwards to the outer surface of the fixed cylinder, the surface of the pull rod at the bottom of the melting groove is provided with a push spring, the bottom of the push spring is fixedly connected with a force application rod, two sides of the force application rod are both provided with oblique planes, and the bottom end of the force application rod is in contact with the inner wall; when the device works, firstly, air in an inoculation chamber is extracted from the one-way valve through air extraction equipment and then sterilized, the sterilized air is refilled into the fixed cylinder, then the device can be carried to a position needing detection outdoors, culture medium is prepared in the melting tank, a reagent to be detected, namely a mixture of a substance to be detected dissolved in water, is injected into the inoculation chamber through the one-way valve, the height of the reagent is larger than that of an inlet, then the pulling rod is moved, the melting tank filled with the culture medium moves towards the right side, the culture medium enters the fixed cylinder, the pulling rods on two sides of the melting tank are just attached to the inner wall of the fixed cylinder, the contact between the air and the culture medium is isolated, and when the pushing spring reaches the position right above the inoculation device, the compressed spring pushes the force application rod to push the extrusion rod in the middle of the E-shaped cylinder downwards, the extrusion stem compresses extrusion spring downwards, simultaneously press the air of extrusion stem bottom to both sides around, the air that is pressed moves makes the catch bar rise, the reagent that can promote a chinese character 'shan' section of thick bamboo inside when the catch bar rises sprays the culture medium in the melting tank from its top bending, after a period, with the catch bar normal position of pushing back again, if there is the escherichia coli in the reagent, then the culture medium can show blue and green, at whole in-process, influence when can reduce bacterium in the air to the inoculation, culture medium and reagent all can not influenced by the bacterium in the air, the precision of detection has been improved.

Preferably, a rotating shaft is arranged in the melting tank, and a stirring plate is arranged on the surface of the rotating shaft; in order to make reagent can reach a chinese character ' shan's section of thick bamboo top and spout under the promotion of catch bar, the internal diameter of the chinese character ' shan's section of thick bamboo of access port top will be less than the diameter of catch bar far away, therefore speed when reagent blowout is very fast, the very fast reagent of spun speed is at first with dial even board contact back, the even board of even board dynamic spindle rotation is dialled in the promotion, on the one hand can make the speed of reagent reduce after dialling even board, on the other hand can dial even board at the rotation in-process, can direct the reagent at one time, prevent that one from spouting when reagent from directly spouting the same position of culture medium, make reagent can spray the culture medium surface by a large area, if there is the escherichia coli, then the area of spilling of escherichia coli is bigger, and then the chromogenic area behind the messenger's culture medium detection escherichia coli is bigger.

Preferably, a circular groove is formed in the position, close to the rotating shaft, of the surface of the uniform stirring plate, the circular groove is used as a divergence center, and a plurality of guide grooves are formed in one end, far away from the rotating shaft, of the uniform stirring plate; after the reagent sprayed from the E-shaped cylinder is sprayed into the circular groove, the reagent is diffused from the center of the circular groove to form a mist reagent, the spraying is more uniform, meanwhile, after a part of the reagent is guided by the plurality of guide grooves in a divergence shape, the reagent passing through the guide grooves and a part of the reagent passing through the surface of the stirring plate can be staggered to a certain degree, when the reagent is sprayed onto the surface of the culture medium, contact points of the reagent and the culture medium are not positioned on the same straight line, the reagent is prevented from generating a cutting effect on the culture medium, the upper surface of the culture medium is loosened, a part of escherichia coli is covered by the loosened culture medium, and the escherichia coli cannot be seen after color development occurs, so that the whole color development intensity is reduced.

Preferably, the distance between two adjacent guide grooves at one end of the edge of the uniform poking plate is larger when the guide grooves are closer to the circular grooves; because with dial the even plate edge more be close to the length of vertically guide way shorter, reagent can break away from earlier and dial even plate surface, causes middle part spun reagent volume many, consequently will keep away from the density setting of the guide way of circular slot more big more, can make the guide way internal blowout's reagent of keeping away from the circular slot the same with the guide way internal blowout's reagent at middle part, improved the degree of consistency that reagent sprayed.

Preferably, the surface of the fixed cylinder at the top of the reaction chamber is embedded with an observation window; after the reagent is sprayed on the surface of the culture medium, in order to prevent air from interfering detection, the pulling rod is pushed to the left side to return to the original position after a certain time is needed, the melting tank can be observed, the pulling rod can be moved to the right after the observation window is added, the culture medium reaches the position right below the observation window, the reaction is observed through the observation window at any time, and the detected result can be observed at the first time after the culture medium and the reagent are fully reacted.

Preferably, as a preferred embodiment of the present invention, the front and back sides of the force application rod are arc-shaped surfaces, and the top ends of the oblique cutting surfaces at the two sides of the force application rod are lower than the top end of the force application rod; set to the scarf from middle part to bottom through the scarf from application of force pole top to bottom, can make application of force pole both sides reserve one section of vertical face, insert the inside back of a chinese character's section of thick bamboo when the application of force pole, when a section of vertical face also entered into a chinese character's section of thick bamboo, when removing the pulling rod again, under the limiting displacement of vertical face, application of force pole can not be through the guide effect of scarf and upward movement, when preventing to stimulate at the excessive speed, the pulling rod is pulled out in the twinkling of an eye after the chinese character's section of thick bamboo top gets into again, then need pull out the back, can rotate the pulling rod, make application of force pole shift out from the chinese character's section of thick bamboo is inside under the effect of arcwall.

The invention has the technical effects and advantages that:

1. according to the rapid test box for escherichia coli, the pulling rod is arranged inside the fixed cylinder, the melting tank is arranged on the pulling rod, after a culture medium is placed inside the melting tank, the pulling rod is moved, the melting tank is sealed inside the fixed cylinder by the pulling rods on the two sides of the melting tank, then a reagent which is not in contact with the outside air is inoculated onto the culture medium through the inoculation device, the culture medium and the reagent are not influenced by bacteria in the air, and the detection precision is improved.

2. According to the escherichia coli rapid test box provided by the invention, the homogenizing plate is arranged in the melting tank, and the sprayed reagent with a higher speed is firstly contacted with the homogenizing plate and then pushed to rotate the rotating shaft, so that the speed of the reagent can be reduced after the homogenizing plate is stirred, and the reagent can be guided by the homogenizing plate in the rotating process, so that the reagent can be sprayed onto the surface of a culture medium in a larger area, the color development area of the culture medium after the escherichia coli is detected is larger, and the color development effect is improved.

Drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a side cross-sectional view of the cassette of the present invention;

FIG. 3 is an enlarged view of portion A of FIG. 1 according to the present invention;

FIG. 4 is a cross-sectional view of the major face of the case of the present invention;

FIG. 5 is a perspective view of the force application rod of the present invention;

in the figure: the inoculation device comprises a box body 1, a box cover 2, a heating chamber 3, an inoculation chamber 4, a reaction chamber 5, a top plate 6, a fixed cylinder 7, an inoculation device 8, a Chinese character 'shan' cylinder 81, an extrusion rod 82, an extrusion spring 83, a push rod 84, an inlet port 85, a one-way valve 9, a melting tank 10, a push spring 11, a force application rod 12, a rotating shaft 13, a stirring plate 14, a circular groove 15, a guide groove 16, an observation window 17 and a pulling rod 18.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1-5, the escherichia coli rapid test kit of the present invention comprises a kit body 1 and a kit cover 2 hinged thereto, wherein the interior of the kit body 1 is divided into a heating chamber 3, an inoculation chamber 4 and a reaction chamber 5 by two partition boards, and is sealed by a top plate 6, and the inoculation chamber 4 is located between the heating chamber 3 and the reaction chamber 5; a fixed cylinder 7 penetrates through the top plate 6, and a pulling rod 18 is inserted into the fixed cylinder 7; the inoculation device 8 is arranged in the inoculation device 8, the inoculation device 8 comprises a Chinese character 'shan' barrel 81, the middle of the Chinese character 'shan' barrel 81 is communicated with the fixed barrel 7, an extrusion rod 82 is arranged in the Chinese character 'shan' barrel 81, the extrusion rod 82 is contacted with the bottom end of the pulling rod 18, the bottom of the extrusion rod 82 is fixedly connected with an extrusion spring 83, push rods 84 are arranged in the Chinese character 'shan' barrel 81 on the front side and the rear side of the extrusion rod 82, the Chinese character 'shan' barrel 81 on the top of the push rods 84 is partially bent and communicated with the top of the fixed barrel 7, and an inlet 85 is formed; a one-way valve 9 is arranged on the wall thickness of the box body 1 at the front side of the inoculation chamber 4, a melting groove 10 is formed in the part, located at the top of the heating chamber 3, of the pulling rod 18, the melting groove 10 extends upwards to the outer surface of the fixed cylinder 7, a pushing spring 11 is arranged on the surface of the pulling rod 18 at the bottom of the melting groove 10, the bottom of the pushing spring 11 is fixedly connected with an application rod 12, two sides of each application rod 12 are arranged to be inclined planes, and the bottom end of each application rod 12 is in contact with the inner wall of the fixed cylinder 7; when the device works, firstly, air in the inoculation chamber 4 is extracted from the one-way valve 9 through air extraction equipment and then sterilized, the sterilized air is refilled into the fixed cylinder 7, then the device can be carried to a position needing detection outdoors, the preparation of a culture medium is carried out in the melting tank 10, a reagent to be detected, namely a mixture of a substance to be detected dissolved in water, is injected into the inoculation chamber 4 through the one-way valve 9, the height of the reagent is larger than that of the inlet 85, then the pulling rod 18 is moved, the melting tank 10 filled with the culture medium moves towards the right side, the culture medium enters the fixed cylinder 7, the pulling rods 18 on the two sides of the melting tank 10 are just attached to the inner wall of the fixed cylinder 7, the contact between the air and the culture medium is isolated, when the pushing spring 11 reaches the position right above the inoculation device 8, the compressed pushing spring 11 pushes the force application rod 12 to push the extrusion rod 82 in the middle of the E-shaped cylinder 81 downwards, the extrusion stem 82 compresses extrusion spring 83 downwards, simultaneously press the air of extrusion stem 82 bottom to the front and back both sides, the air that is pressed makes catch bar 84 rise, can promote when catch bar 84 rises on the inside reagent of a chinese character 'shan' section of thick bamboo 81 sprays the culture medium in melting tank 10 from its top bending, after a period, with pull rod 18 back home position again, if there is the escherichia coli in the reagent, then the culture medium can develop blue-green, in whole process, can reduce the influence of bacterium when the inoculation in the air, culture medium and reagent all can not influenced by the bacterium in the air, the precision of detection has been improved.

A rotating shaft 13 is arranged in the melting tank 10, and a stirring plate 14 is arranged on the surface of the rotating shaft 13; in order to enable the reagent to reach the top end of the E-shaped cylinder 81 under the pushing of the push rod 84 and be sprayed out, the inner diameter of the E-shaped cylinder 81 above the inlet port 85 is far smaller than the diameter of the push rod 84, so that the speed of the reagent during spraying is high, after the reagent with high spraying speed is firstly contacted with the uniform stirring plate 14, the uniform stirring plate 14 is pushed to drive the rotating shaft 13 to rotate, on one hand, the speed of the reagent can be reduced after the uniform stirring plate 14 is stirred, on the other hand, the uniform stirring plate 14 can guide the reagent in the rotating process, one time of spraying the reagent directly to the same position of a culture medium is prevented, the reagent can be sprayed to the surface of the culture medium in a large area, if the Escherichia coli exists, the spraying area of the Escherichia coli is larger, further, the color development area of the culture medium after the Escherichia coli is detected is larger, and the color.

A circular groove 15 is formed in the surface of the uniform stirring plate 14 close to the rotating shaft 13, the circular groove 15 is used as a divergence center, and a plurality of guide grooves 16 are formed in one end, far away from the rotating shaft 13, of the uniform stirring plate 14; after the reagent sprayed from the E-tube 81 is sprayed into the circular groove 15, the reagent is diffused from the center of the circular groove 15 to form a mist reagent, the spraying is more uniform, meanwhile, a part of the reagent is guided by the plurality of guide grooves 16 in a divergence shape, the reagent passing through the guide grooves 16 and a part of the reagent passing through the surface of the stirring plate 14 can be staggered to a certain degree, when the reagent is sprayed onto the surface of the culture medium, contact points of the reagent and the culture medium are not positioned on the same straight line, the reagent is prevented from generating a cutting effect on the culture medium, the upper surface of the culture medium is loosened, a part of escherichia coli is covered by the loosened culture medium, and the escherichia coli cannot be seen after color development occurs, so that the whole color development intensity is reduced.

The distance between two adjacent guide grooves 16 at one end of the edge of the uniform poking plate 14 is larger when the guide grooves are closer to the circular groove 15; because with dial even board 14 edge more nearly vertical guide way 16's length shorter, reagent can break away from earlier and dial even board 14 surface, causes the spun reagent in middle part many, consequently will keep away from the more big that the density of the guide way 16 of circular slot 15 sets up, can make and keep away from spun reagent in the guide way 16 of circular slot 15 and the guide way 16 in middle part the same, improved the degree of consistency that reagent sprayed.

An observation window 17 is embedded in the surface of the fixed cylinder 7 at the top of the reaction chamber 5; after the reagent is sprayed on the surface of the culture medium, in order to prevent air from interfering detection, the pulling rod 18 is pushed to the left side to return to the original position after a certain period of time is needed to observe the melting tank 10, the pulling rod 18 can be moved to the right after the observation window 17 is added, the culture medium reaches the position right below the observation window 17, the reaction is observed through the observation window 17 at any time, and the detected result can be observed at the first time after the culture medium and the reagent are fully reacted.

As a preferred embodiment of the present invention, the front and back sides of the force application rod 12 are arc-shaped surfaces, and the top ends of the oblique cutting surfaces at the two sides of the force application rod 12 are lower than the top end of the force application rod 12; set up to the scarf from the middle part to the bottom through the scarf from the application of force pole 12 top to bottom, can make application of force pole 12 both sides reserve one section vertical face, after application of force pole 12 inserts a chinese character's section of thick bamboo 81 inside, when a section vertical face also enters into a chinese character's section of thick bamboo 81, when removing pull rod 18 again, under the limiting displacement of vertical face, application of force pole 12 can not be through the guide effect of scarf and upward movement, when preventing to pull at the excessive speed, pull rod 18 is pulled out in the twinkling of an eye again after the chinese character's section of thick bamboo 81 top gets into, then need pull out the back, can rotate pull rod 18, make application of force pole 12 shift out from chinese character's section of thick bamboo 81 inside under the effect of arcwall.

When the device works, firstly, air in the inoculation chamber 4 is extracted from the one-way valve 9 through air extraction equipment and then sterilized, the sterilized air is refilled into the fixed cylinder 7, then the device can be carried to a position needing detection outdoors, the preparation of a culture medium is carried out in the melting tank 10, a reagent to be detected, namely a mixture of a substance to be detected dissolved in water, is injected into the inoculation chamber 4 through the one-way valve 9, the height of the reagent is larger than that of the inlet 85, then the pulling rod 18 is moved, the melting tank 10 filled with the culture medium moves towards the right side, the culture medium enters the fixed cylinder 7, the pulling rods 18 on the two sides of the melting tank 10 are just attached to the inner wall of the fixed cylinder 7, the contact between the air and the culture medium is isolated, when the pushing spring 11 reaches the position right above the inoculation device 8, the compressed pushing spring 11 pushes the force application rod 12 to push the extrusion rod 82 in the middle of the E-shaped cylinder 81 downwards, the extrusion stem 82 compresses extrusion spring 83 downwards, simultaneously press the air of extrusion stem 82 bottom to the front and back both sides, the air that is pressed makes catch bar 84 rise, can promote when catch bar 84 rises on the inside reagent of a chinese character 'shan' section of thick bamboo 81 sprays the culture medium in melting tank 10 from its top bending, after a period, with pull rod 18 back home position again, if there is the escherichia coli in the reagent, then the culture medium can develop blue-green, in whole process, can reduce the influence of bacterium when the inoculation in the air, culture medium and reagent all can not influenced by the bacterium in the air, the precision of detection has been improved.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.