阅读说明：本技术 一种鲜切果蔬中食源性病原微生物的多重pcr检测用卡盒 (Multiple PCR detection card box for food-borne pathogenic microorganisms in fresh-cut fruits and vegetables ) 是由 王记莲 于 2019-10-21 设计创作，主要内容包括：本发明公开了一种鲜切果蔬中食源性病原微生物的多重PCR检测用卡盒,包括底板和检测盒,所述底板位于检测盒的下端内壁上,所述底板的上端侧壁卡接连接有收纳槽,所述收纳槽的上端侧壁开设有开口,所述收纳槽的上端侧壁通过铰链转动连接有与开口匹配的密封盖,所述密封盖上设有锁紧装置,所述密封盖的上端侧壁固定连接有把手,所述所述检测盒的上端侧壁设有开口朝下的收纳盒,所述收纳盒的上端内壁固定连接有电动伸缩杆,所述电动伸缩杆的伸缩端固定连接有夹紧装置,所述检测盒的侧壁设有取放门。本发明通过收纳槽与底板等结构之间的配合能够快速安全地将待检物品放到检测盒中,不会有外界的病原微生物附着到待检物品上,安全高效。(The invention discloses a card box for multiple PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables, which comprises a bottom plate and a detection box, wherein the bottom plate is positioned on the inner wall of the lower end of the detection box, the side wall of the upper end of the bottom plate is connected with a containing groove in a clamping manner, the side wall of the upper end of the containing groove is provided with an opening, the side wall of the upper end of the containing groove is rotatably connected with a sealing cover matched with the opening through a hinge, the sealing cover is provided with a locking device, the side wall of the upper end of the sealing cover is fixedly connected with a handle, the side wall of the upper end of the detection box is provided with a containing box with a downward opening, the inner wall of the upper end of the containing box is fixedly connected with an electric telescopic rod. According to the invention, the accommodating groove and the bottom plate can be matched to quickly and safely place the object to be detected in the detection box, so that no external pathogenic microorganism is attached to the object to be detected, and the detection box is safe and efficient.)

1. The card box for the multiple PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables comprises a bottom plate (1) and a detection box (2), wherein the bottom plate (1) is positioned on the inner wall of the lower end of the detection box (2), and is characterized in that a receiving groove (3) is connected to the side wall of the upper end of the bottom plate (1) in a clamping manner, an opening is formed in the side wall of the upper end of the receiving groove (3), a sealing cover (4) matched with the opening is rotatably connected to the side wall of the upper end of the receiving groove (3) through a hinge, a locking device (5) is arranged on the sealing cover (4), a handle (6) is fixedly connected to the side wall of the upper end of the sealing cover (4), a receiving box (7) with a downward opening is arranged on the side wall of the upper end of the detection box (2), an electric telescopic rod (8) is fixedly connected to the inner wall of, a taking and placing door (9) is arranged on the side wall of the detection box (2), and a detection device (10) is fixedly connected to the inner wall, far away from the taking and placing door (9), of the detection box (2);

the equal fixedly connected with fixed block of both sides lateral wall (11) of bottom plate (1), the mounting groove has all been seted up to the both sides lateral wall of accomodating groove (3), fixedly connected with slide bar (12) between the both sides lateral wall of mounting groove, the sliding sleeve is equipped with sliding sleeve (13) on slide bar (12), the lateral wall fixedly connected with mount pad (14) of sliding sleeve (13), mount pad (14) run through the opening of mounting groove, be located outside the mounting groove the vertical tight set bolt (15) that is equipped with of upper end lateral wall of mount pad (14), the thread groove has been seted up to the upper end lateral wall of fixed block (11), tight set bolt (15) run through mount pad (14) and extend to in the thread groove.

2. The cartridge for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein the side wall of the upper end of the sliding sleeve (13) is fixedly connected with the side wall of the upper end of the mounting groove through a return spring (16), and the return spring (16) is sleeved on the sliding rod (12).

3. The cartridge for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein the clamping device comprises a housing (17), the housing (17) is of a hollow structure, a forward and reverse motor (18) is fixedly connected to a side wall of the housing (17), an output shaft of the forward and reverse motor (18) penetrates through the side wall of the housing (17) and is fixedly connected with a gear (19), two racks (20) matched with the gear (19) are slidably connected between inner walls of two sides of the housing (17), the two racks (20) are respectively located at upper and lower sides of the gear (19), a connecting rod (21) is fixedly connected to a side wall of the rack (20) far away from the gear (19), two strip-shaped openings are formed in a side wall of the lower end of the housing (17), and are respectively located at two sides of the gear (19), two connecting rod (21) run through two bar mouths respectively, are located outside casing (17) the lower extreme lateral wall fixedly connected with wedge (22) of connecting rod (21).

4. The cartridge for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein a cushion pad (23) is fixedly connected to the inner wall of the storage case (7), the cushion pad (23) is wave-shaped, the connection between the storage case (7) and the detection case (2) is hermetically arranged, the storage case (7) penetrates through the upper end side wall of the detection case (2), and the inner diameter width of the storage case (7) is larger than the outer diameter width of the storage groove (3).

5. The multiple PCR detection card box for detecting the food-borne pathogenic microorganisms in the fresh-cut fruits and vegetables according to claim 1, wherein the side wall of the lower end of the accommodating groove (3) is fixedly connected with a sealing ring (24), the sealing ring (24) is made of rubber, and the side wall of the upper end of the bottom plate (1) is provided with a sealing groove matched with the sealing ring (24).

6. The cartridge for the multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein an installation opening is formed in the side wall of the cartridge (2) far away from the detection device (10), rubber gloves (29) matched with the installation opening are fixedly connected to the inner wall of the cartridge (2), and the connection part of the rubber gloves (29) and the installation opening is sealed.

7. The cartridge for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein an observation port is formed in the side wall of the cartridge (2), and a toughened glass plate (25) is hermetically connected in the observation port.

8. The cartridge for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to claim 1, wherein four adjustable support legs (26) are fixedly connected to the side wall of the lower end of the detection cartridge (2), a level gauge (27) is fixedly connected to the side wall of the upper end of the detection cartridge (2), a limiting strip (28) is fixedly connected to the inner wall of the lower end of the detection cartridge (2), and the side wall of the limiting strip (28) abuts against the side wall of the bottom plate (1).

Technical Field

The invention relates to the technical field of microbial detection, in particular to a card box for multiple PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables.

Background

Fresh-cut fruits and vegetables, also called minimum processed fruits and vegetables, semi-processed fruits and vegetables, slightly processed fruits and vegetables and the like, are instant or ready-to-use fruit and vegetable products which are prepared by taking fresh fruits and vegetables as raw materials, carrying out a series of treatments such as grading, cleaning, trimming, peeling, cutting, fresh keeping, packaging and the like, and then entering a refrigerator for sale through low-temperature transportation, wherein the fresh-cut fruits and vegetables are exposed in the air in the selling process and have certain eating risks due to the attachment of microorganisms to the fresh-cut fruits and vegetables.

The polymerase chain reaction uses a piece of DNA as a template, and the piece of DNA is amplified to a sufficient amount in the presence of DNA polymerase and nucleotide substrates for structural and functional analysis. The principle of PCR is used to amplify a DNA fragment located between two known sequences, similar to the process of replication of natural DNA. The DNA molecule to be amplified is taken as a template, a pair of oligonucleotide fragments which are respectively complementary with the 5 'end and the 3' end of the template are taken as primers, under the action of DNA polymerase, the DNA fragments are extended along the template chain according to a semi-reserved replication mechanism until new DNA synthesis is completed, and the process is repeated, so that the target DNA fragments can be amplified, and whether pathogenic microorganisms exist in fresh-cut fruits and vegetables or not is detected by a PCR (polymerase chain reaction) technology.

Disclosure of Invention

The invention aims to solve the problems that in the prior art, fresh-cut fruits and vegetables are always exposed in the air and possibly infected with pathogenic microorganisms on a transportation road, and when the fresh-cut fruits and vegetables are sampled to prepare samples, the fresh-cut fruits and vegetables are also exposed in the air, so that the risk of being infected with pathogenic pathogens is caused, and the detection accuracy is further reduced.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a multiple PCR of food source pathogenic microorganism detects uses card box in fresh-cut fruit vegetables, includes bottom plate and detection box, the bottom plate is located the lower extreme inner wall that detects the box, the upper end lateral wall joint of bottom plate is connected with accomodates the groove, the opening has been seted up to the upper end lateral wall of accomodating the groove, the upper end lateral wall of accomodating the groove is rotated through the hinge and is connected with the sealed lid that matches with the opening, sealed covering is equipped with locking device, the upper end lateral wall fixedly connected with handle of sealed lid, the upper end lateral wall that detects the box is equipped with opening receiver down, the upper end inner wall fixedly connected with electric telescopic handle of receiver, electric telescopic handle's flexible end fixedly connected with clamping device, the lateral wall that detects the box is equipped with gets and puts the door, the detection box is kept away from.

Preferably, the equal fixedly connected with fixed block of both sides lateral wall of bottom plate, the mounting groove has all been seted up to the both sides lateral wall of accomodating the groove, fixedly connected with slide bar between the both sides lateral wall of mounting groove, the sliding sleeve is equipped with the sliding sleeve on the slide bar, the lateral wall fixedly connected with mount pad of sliding sleeve, the mount pad runs through the opening of mounting groove, is located outside the mounting groove the vertical tight set bolt that is equipped with of upper end lateral wall of mount pad, the thread groove has been seted up to the upper end lateral wall of fixed block, tight set bolt runs through the mount pad and extends to in the thread groove.

Preferably, the upper end lateral wall of sliding sleeve passes through reset spring and the upper end lateral wall fixed connection of mounting groove, and reset spring cover locates on the slide bar.

Preferably, clamping device includes the casing, the casing is hollow structure, the lateral wall fixedly connected with positive and negative motor of casing, the output shaft of positive and negative motor runs through the lateral wall and the fixedly connected with gear of casing, sliding connection has two racks that match with the gear, two between the both sides inner wall of casing the rack is located the upper and lower both sides of gear respectively, the lateral wall fixedly connected with connecting rod of gear is kept away from to the rack, two bar mouths have been seted up to the lower extreme lateral wall of casing, two the bar mouth is located the both sides of gear respectively, two the connecting rod runs through two bar mouths respectively, is located outside the casing the lower extreme lateral wall fixedly connected with wedge of connecting rod.

Preferably, the inner wall fixedly connected with blotter of receiver, the blotter is the wave setting, the sealed setting of junction of receiver and detection box, and the receiver runs through the upper end lateral wall of detection box, the internal diameter width of receiver is greater than the external diameter width who accomodates the groove.

Preferably, the lower extreme lateral wall fixedly connected with sealing ring of holding the groove, the material of sealing ring is rubber, the seal groove that matches with the sealing ring is seted up to the upper end lateral wall of bottom plate.

Preferably, the detection box is far away from the lateral wall of detection device and has been seted up the installing port, the inner wall fixedly connected with of detection box and the rubber gloves of installing port matching, and the sealed setting of junction of rubber gloves and installing port.

Preferably, the side wall of the detection box is provided with an observation port, and a toughened glass plate is hermetically connected in the observation port.

Preferably, the lower extreme lateral wall fixedly connected with four adjustable stabilizer blades of detection box, and the upper end lateral wall fixedly connected with spirit level of detection box, the lower extreme inner wall fixedly connected with spacing of detection box, the lateral wall of spacing offsets with the lateral wall of bottom plate.

Compared with the prior art, the invention has the beneficial effects that:

1. when fresh-cut fruits and vegetables are conveyed, the fruits and vegetables are placed in the bottom plate and the accommodating groove, so that pathogenic microorganisms are prevented from attaching to an object to be detected in the conveying process, the accommodating groove is placed in the detection box, then the clamping device clamps the handle, the accommodating groove is separated from the bottom plate, the object to be detected is taken out, the detection device is used for detection, the structure is simple, the detection quality is guaranteed, and the taking is convenient;

2. utilize the cooperation between devices such as mounting groove, sliding sleeve, slide bar and tight set bolt to use, can will accomodate groove and bottom plate installation dismantlement fast, convenient operation guarantees moreover that there is not pathogenic microorganism to get into and accomodates the groove, in addition, utilizes positive and negative motor, gear, rack, connecting rod and wedge isotructure's cooperation can the tight handle of quick clamp to accomodate groove and bottom plate separation, guarantee that article on the bottom plate accept smoothly and detect.

Drawings

FIG. 1 is a schematic structural diagram of a cassette for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to the present invention;

FIG. 2 is a side view of the cassette for multiplex PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables according to the present invention;

FIG. 3 is a schematic view of the structure at A in FIG. 2;

fig. 4 is a schematic diagram of the structure at B in bitmap 2.

In the figure: the device comprises a base plate 1, a detection box 2, a storage groove 3, a sealing cover 4, a locking device 5, a handle 6, a storage box 7, an electric telescopic rod 8, a taking and placing door 9, a detection device 10, a fixing block 11, a sliding rod 12, a sliding sleeve 13, a mounting seat 14, a fastening bolt 15, a return spring 16, a shell 17, a positive and negative motor 18, a gear 19, a rack 20, a connecting rod 21, a wedge block 22, a cushion pad 23, a sealing ring 24, a toughened glass plate 25, an adjustable supporting leg 26, a level gauge 27, a limiting bar 28 and a rubber glove 29.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-4, a card box for multiple PCR detection of food-borne pathogenic microorganisms in fresh-cut fruits and vegetables comprises a bottom plate 1 and a detection box 2, wherein a mounting opening is formed in the side wall, far away from a detection device 10, of the detection box 2, a rubber glove 29 matched with the mounting opening is fixedly connected to the inner wall of the detection box 2, the joint of the rubber glove 29 and the mounting opening is hermetically arranged, a sample in the detection box 2 can be rapidly operated by using the rubber glove 29, the external pathogenic microorganisms are not attached to the sample, the safety and the high efficiency are realized, an observation opening is formed in the side wall of the detection box 2, a toughened glass plate 25 is hermetically connected to the observation opening, the specific condition of the sample in the detection box 2 can be rapidly observed through the toughened glass plate 25, four adjustable support legs 26 are fixedly connected to the side wall at the lower end of the detection box 2, and a level meter 27 is, the angle of the detection box 2 can be conveniently adjusted by using the adjustable supporting feet 26, so that the stability of the sample placed in the detection box 2 is ensured;

the inner wall of the lower end of the detection box 2 is fixedly connected with a limiting strip 28, the side wall of the limiting strip 28 is abutted against the side wall of the bottom plate 1, the position of the bottom plate 1 is limited by the limiting strip 28, the clamping device can stably clamp the handle 6, the side walls of the two sides of the bottom plate 1 are fixedly connected with fixed blocks 11, the side walls of the two sides of the accommodating groove 3 are respectively provided with a mounting groove, a slide rod 12 is fixedly connected between the side walls of the two sides of the mounting groove, a slide sleeve 13 is slidably sleeved on the slide rod 12, the side wall of the upper end of the slide sleeve 13 is fixedly connected with the side wall of the upper end of the mounting groove through a reset spring 16, the reset spring 16 is sleeved on the slide rod 12, the side wall of the slide sleeve 13 is fixedly connected with a mounting seat 14, the mounting seat 14 penetrates through the opening of the mounting groove, the side wall of the upper end of the, the accommodating groove 3 and the bottom plate 1 can be quickly assembled and disassembled by utilizing the matching use of the mounting groove, the sliding sleeve 13, the sliding rod 12, the fastening bolt 15 and other devices, the operation is convenient, and pathogenic microorganisms can not enter the accommodating groove 3;

the bottom plate 1 is positioned on the inner wall of the lower end of the detection box 2, the upper end side wall of the bottom plate 1 is connected with the containing groove 3 in a clamping manner, the lower end side wall of the containing groove 3 is fixedly connected with a sealing ring 24, the sealing ring 24 is made of rubber, the upper end side wall of the bottom plate 1 is provided with a sealing groove matched with the sealing ring 24, the sealing groove and the sealing ring 24 are matched to ensure that no external pathogenic microorganism enters the containing groove 3 after the fastening bolt 15 is separated from the thread groove, the upper end side wall of the containing groove 3 is provided with an opening, the upper end side wall of the containing groove 3 is rotatably connected with a sealing cover 4 matched with the opening through a hinge, the sealing cover 4 is provided with a locking device 5, a sample can be conveniently placed on the bottom plate 1 after the sealing cover 4 is opened, the locking device 5 can lock the sealing cover 4, the sealing cover 4, the side wall of the upper end of the sealing cover 4 is fixedly connected with a handle 6, and the accommodating groove 3 can be conveniently driven to move by lifting the handle 6, so that time and labor are saved;

the side wall of the upper end of the detection box 2 is provided with a storage box 7 with a downward opening, the inner wall of the storage box 7 is fixedly connected with a cushion pad 23, the cushion pad 23 is arranged in a wave shape, the joint of the storage box 7 and the detection box 2 is arranged in a sealing way, the storage box 7 penetrates through the side wall of the upper end of the detection box 2, the inner diameter width of the storage box 7 is larger than the outer diameter width of the storage groove 3, the inner wall of the upper end of the storage box 7 is fixedly connected with an electric telescopic rod 8, the telescopic end of the electric telescopic rod 8 is fixedly connected with a clamping device, the clamping device comprises a shell 17, the shell 17 is of a hollow structure, the side wall of the shell 17 is fixedly connected with a positive and negative motor 18, the output shaft of the positive and negative motor 18 penetrates through the side wall of the shell 17 and is fixedly connected with a gear 19, two bar mouths have been seted up to casing 17's lower extreme lateral wall, two bar mouths are located gear 19's both sides respectively, two connecting rods 21 run through two bar mouths respectively, the lower extreme lateral wall fixedly connected with wedge 22 that is located casing 17 outer connecting rod 21, utilize positive and negative motor 18, gear 19, rack 20, connecting rod 21 and wedge 22 isotructure's cooperation can the rapid clamping handle 6, thereby mention and accomodate groove 3 and bottom plate 1 separation, guarantee that the article on the bottom plate 1 accept the detection smoothly safely, the lateral wall that detects box 2 is equipped with gets and puts door 9, close and get and put door 9, use detection device 10 to detect the sample, it keeps away from the inner wall fixedly connected with detection device 10 who gets and put door 9 to detect box 2.

When the invention needs to take a sample, the accommodating groove 3 is taken out of the detection box 2, then the sample is taken in places such as supermarkets, shops and the like, the sealing door is opened, the sample to be detected is placed in the accommodating groove 3 and then is transported to the detection center, the sample is ensured to be in a sealing condition in the transporting process, so that the external pathogenic microorganisms are prevented from being attached to the sample, after the sample is transported to the detection center, the fastening bolt 15 is screwed, the fastening bolt 15 is separated from the thread groove, so that the accommodating groove 3 can be taken off from the bottom plate 1, then the electric telescopic rod 8 is descended, the handle 6 is clamped by the clamping device, so that the accommodating groove 3 is lifted into the accommodating box 7, the sample is exposed in the detection box 2, the detection device 10 can be used for rapidly detecting the sample, the operation is convenient and rapid, and no external pathogenic microorganisms are attached to the sample in the transporting or detecting process, the quality of detection is guaranteed.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.