Flow injection method for uniformly distributing reagent
阅读说明：本技术 一种均匀分布试剂的流动注射法 (Flow injection method for uniformly distributing reagent ) 是由 桑泉 童海明 姜飞 刘鹏 王景桦 于 2019-10-10 设计创作，主要内容包括：本发明涉及海洋检测技术领域,具体为一种均匀分布试剂的流动注射法。本发明试样的动力系统采用注射量可控制、液体流动方向可控制的泵阀,试剂的动力系统均采用注入量可控制的泵阀,单次注样可控可调,通过连续不断的重复注样,在整个液路系统中实现液体的持续流动,通过保持单次注样时,试剂在试样中均匀分布,确保混合溶液在测量通道内的一致性,且此一致性与测量通道长度无关。本发明通过控制试剂注入时间,使试剂在试样中均匀分布,从而确保混合溶液在测量通道内的一致性,且此一致性与测量通道长度无关,控制方法简单,容易实现。(The invention relates to the technical field of ocean detection, in particular to a flow injection method for uniformly distributing a reagent. The power system of the sample adopts the pump valve with controllable injection amount and controllable liquid flowing direction, the power system of the reagent adopts the pump valve with controllable injection amount, the single sample injection is controllable and adjustable, the continuous liquid flowing in the whole liquid path system is realized through continuous repeated sample injection, and the reagent is uniformly distributed in the sample when the single sample injection is kept, so that the consistency of the mixed solution in the measuring channel is ensured, and the consistency is irrelevant to the length of the measuring channel. The invention ensures that the reagent is uniformly distributed in the sample by controlling the injection time of the reagent, thereby ensuring the consistency of the mixed solution in the measuring channel, and the consistency is irrelevant to the length of the measuring channel.)
1. A flow injection method for uniformly distributing a reagent, comprising the steps of:
1) adjusting the volume V of single injection of the sample according to the test requirement to ensure thatIs an integer to ensure that the signal has the same concentration fluctuation period in the detection channel at each measurement, wherein the optical path length LLight (es)The length of a detection channel of liquid in a photoelectric detection module is shown, and R is the inner diameter of a pipeline after a sample and a reagent are mixed;
2) determining a single injection period according to the single injection volume V and the injection rate V of the sample,wherein tau isSample liquidFor time delay of the sample due to the liquid path, τSample electricityControllable delay of the sample;
3) controlling the injection duration of the reagent in a single sample injection period to satisfy TAgent for treating cancer-τLiquid medicine-τChemical and electric energy meter＝TSample (A)-τSample liquid-τSample electricitySo that it is at the same time as the sample injection time, ensuring that the reagent is uniformly distributed in the sample, where τLiquid medicineComprises two parts of pump valve switch time delay and liquid path length time delay, tauChemical and electric energy meterIn order to meet the timing requirements for controllable delay.
2. The flow injection method of uniformly distributing a reagent according to claim 1,
when a plurality of reagents with the same injection amount need to be injected, the injection time of each reagent in a single sample injection period is the same as the sample injection time, so that the reagents can be uniformly distributed in the sample.
3. The flow injection method for uniformly distributing reagent according to claim 1, wherein when a plurality of reagents with different injection amounts are required to be injected, the method comprises the following steps:
1) adjusting the concentration of the reagent to make the injection amount of each reagent the same;
2) the injection time of each reagent in a single sample injection cycle is controlled to be the same as the sample injection time.
4. The flow injection method for uniformly distributing reagent according to claim 1, wherein when a plurality of reagents with different injection amounts are required to be injected, the method comprises the following steps:
the quantitative controllable component of the plunger pump is adopted to adjust the sample introduction proportion of the plunger pump of various reagents, so that the injection time of various reagents in a single sample injection period is the same as the sample injection time, and the reagents can be uniformly distributed in the sample.
Technical Field
The invention relates to the technical field of ocean detection, in particular to a flow injection method for uniformly distributing a reagent.
Background
A water quality detector is a chemical analysis instrument for detecting the concentration of different substances in a water body, and is used for measuring the content of various trace elements in drinking water, detecting whether the discharge of various factories generating seriously polluted water bodies reaches the standard, checking the treatment effect of a sewage treatment plant, observing the pollution condition in fresh water of rivers, lakes and the like, and observing the distribution, the change and the like of various nutrient salt components in seawater.
When environmental analysis is performed in the ocean, the environmental analyzer has its own uniqueness due to the unique requirements of the ocean application: 1) no gas circuit is arranged; 2) the influence of bubbles is remarkably increased; 3) and a liquid path filled all the time.
The ocean-oriented environment analyzer has a large gap in the accuracy and stability of measurement, and the factors causing such problems include the following:
1) as described above, the air bubbles are unavoidable factors of the marine environment analyzer, and are important factors affecting the numerical stability of the analyzer. The influence can be reduced by liquid path design or adding a defoaming agent and the like;
2) the influence of biological adhesion and the biological adhesion phenomenon are problems which all marine instruments need to face, an effective mode is not completely avoided at present, and in an environment analyzer, proper liquid path design and an injection mode can be selected for reduction;
3) and the influence of chemical reagent imbalance is detected by using a photometry, a plurality of reagents are used under most conditions, and according to the requirements of chemical reactions, the reagents need to be injected into the water body to be detected in a certain sequence, and the injection time interval and the reagent amount injected each time also need to meet certain requirements. For example, in some methods for detecting silicate content, 3 chemicals are used, with a ratio of 1: 1: and 2, injecting the chemical reagents into the sample respectively in sequence, wherein the injection interval of 1 reagent and 3 reagents is respectively 20 seconds and 40 seconds.
As mentioned above, most of the marine-oriented instruments adopt continuous measurement and use the flow injection method, and the chemical reaction is performed in the corresponding pipeline. In actual use, it is found that the degree of chemical reaction fluctuates to various degrees. In the same multi-parameter instrument, the fluctuation of some parameter chemical reactions is larger, and the fluctuation of some parameter chemical reactions is smaller. When a plurality of same instruments are compared, the fluctuation of the same parameters is also found, and the rules among different parameters and among the same parameters are difficult to master.
Such fluctuation and the influence of the bubbles on the instrument are combined with each other, which further causes instability of the measured value, and the generation conditions of the two influencing factors are random, which brings great difficulty to the post-processing.
In the flow injection method, there is no special module for storing a certain amount of sample and reagent, and there is no special module for chemical reaction, and the sample and reagent are continuously flowed in the pipeline. Reagents are injected into the sample at specific points in time as required by the chemical reaction. Similarly, the sample that has completed the chemical reaction will flow continuously, and flow in one direction in the detection module, entering at one end, and flowing out at one end.
In the present stage, the injection of the reagent is performed in a time-sharing manner, namely, the injection is performed for a short time at certain specific points, and the injection is closed in other time periods. When only one reagent is required for the reaction, it is apparent that the chemical reaction proceeds only at the reagent injection point, and the other points are not changed, resulting in non-uniformity of the chemical reaction. When two or more reagents are required for the reaction, it is very easy to create a problem that the two reagents may not meet each other at all, so there may be several cases in the liquid path: there were no reagents at all, only reagent 1, only reagent 2, and both. Therefore, it is more simple to think that the chemical reaction is not complete in the final liquid path.
The electronic sampling speed is very fast, the commonly used sampling rate is dozens of ksps or even dozens of Msps, and the high sampling rate is very difficult to control when the sampling is required to be performed at the stage of the same reaction degree every time.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a flow injection method for uniformly distributing a reagent, which can meet the stability requirement on a measured value by ensuring the stability of chemical components in a measuring optical path and adjusting the sampling time.
(II) technical scheme
The invention provides the following technical scheme:
a flow injection method for uniformly distributing a reagent, comprising the steps of:
1) adjusting the volume V of single injection of the sample according to the test requirement to ensure that
2) determining a single injection period according to the single injection volume V and the injection rate V of the sample,
3) controlling the injection duration of the reagent in a single sample injection period to satisfy TAgent for treating cancer-τLiquid medicine-τChemical and electric energy meter＝TSample (A)-τSample liquid-τSample electricitySo that it is at the same time as the sample injection time, ensuring that the reagent is uniformly distributed in the sample, where τLiquid medicineComprises two parts of pump valve switch time delay and liquid path length time delay, tauChemical and electric energy meterIn order to meet the timing requirements for controllable delay.
Furthermore, when a plurality of reagents with the same injection amount need to be injected, the injection time of each reagent in a single sample injection period is the same as the sample injection time, so that the reagents can be uniformly distributed in the sample.
Further, when a plurality of reagents with different injection amounts need to be injected, the method comprises the following steps:
1) adjusting the concentration of the reagent to make the injection amount of each reagent the same;
2) the injection time of the reagent of the smallest injection amount in a single sample injection period is controlled to be the same as the sample injection time.
Further, when a plurality of reagents with different injection amounts need to be injected, the method comprises the following steps:
the quantitative controllable component of the plunger pump is adopted to adjust the sample introduction proportion of the plunger pump of various reagents, so that the injection time of various reagents in a single sample injection period is the same as the sample injection time, and the reagents can be uniformly distributed in the sample.
(III) advantageous effects
Compared with the prior art, the invention provides a novel anti-counterfeiting method, which has the following beneficial effects:
1. the reagent is uniformly distributed in the sample by controlling the injection time of the reagent, so that the consistency of the mixed solution in the measuring channel is ensured, the consistency is irrelevant to the length of the measuring channel, and the control method is simple and easy to realize;
2. the universality is good and is not influenced by specific chemical reaction conditions.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
- 上一篇：一种医用注射器针头装配设备
- 下一篇：一种定时注入试剂的流动注射法