阅读说明：本技术 一种定时注入试剂的流动注射法 (Flow injection method for injecting reagent at fixed time ) 是由 童海明 桑泉 刘鹏王 王景桦 任海元 于 2019-10-10 设计创作，主要内容包括：本发明涉及海洋检测技术领域,具体公布了一种定时注入试剂的流动注射法,通过控制试剂在单次注入过程中的时间,即控制试剂在单次注入过程中在试样当中的位置,试样和试剂的动力系统均采用可定量的泵阀系统,单次注样过程可控可调,通过连续不断的重复注样,在整个液路系统中实现液体的持续流动,通过确保测量光程内的化学成分稳定,并调节采样时间,即可满足对测量值的稳定性要求。(The invention relates to the technical field of ocean detection, and particularly discloses a flow injection method for injecting a reagent at regular time.)

1. A flow injection method for timed injection of a reagent, comprising the steps of:

1) adjusting the volume V of single injection of the sample according to the test requirement to ensure that

2) determining a single injection period according to the single injection volume V and the injection rate V of the sample,

3) controlling the injection time of the reagent in a single sample injection period to satisfy t₀＝t_{Liquid for treating urinary tract infection}+t_{Electric power}The injection time of the reagent in each single injection period is kept consistent, so that the position of the reagent in the sample in the single injection process is kept consistent, wherein t_{Liquid for treating urinary tract infection}Is a delay caused by the fluid path, t_{Electric power}Is a controllable delay added to meet timing requirements.

2. The flow injection method for timed injection of reagents according to claim 1, characterized in that,

when a plurality of reagents with the same injection amount need to be injected, the time delay parameter t of each reagent is adjusted_{Electric power}Let t be_{1 liquid}+t_{1 electricity}＝t_{Liquid 2}+t_{n electricity}To ensure that the reagents meet at the same point in time during each injection cycle so that the reagent mixing point of the mixed liquid is fixed in position within the optical detection module channel after each single injection of the sample.

3. The flow injection method for injecting reagents at regular intervals according to claim 1, wherein when a plurality of reagents with different injection amounts are required to be injected, the method comprises the steps of:

1) adjusting the time delay parameter t of the reagent_{Electric power}So that the various reagents can meet at the same point in time, t, of each injection cycle_{1 liquid}+t_{1 electricity}＝t_{Liquid 2}+t_{2 electricity}＝t_{n liquid}+t_{n electricity}So that the position of the reagent mixing point of the mixed liquid in the channel of the optical detection module is fixed after each single sample injection;

2) determining the opening time t of the pump valve of each reagent according to different single injection amount of each reagent₁To t_n；

3) Adjusting the delay parameter t of the various reagents_{1 electricity}To t_{n electricity}To make

Technical Field

The invention relates to the technical field of ocean detection, in particular to a flow injection method for injecting a reagent at regular time.

Background

A water quality detector is a chemical analysis instrument for detecting the concentration of different substances in a water body, and is used for measuring the content of various trace elements in drinking water, detecting whether the discharge of various factories generating seriously polluted water bodies reaches the standard, checking the treatment effect of a sewage treatment plant, observing the pollution condition in fresh water of rivers, lakes and the like, and observing the distribution, the change and the like of various nutrient salt components in seawater.

When environmental analysis is performed in the ocean, the environmental analyzer has its own uniqueness due to the unique requirements of the ocean application: 1) no gas circuit is arranged; 2) the influence of bubbles is remarkably increased; 3) and a liquid path filled all the time.

The ocean-oriented environment analyzer has a large gap in the accuracy and stability of measurement, and the factors causing such problems include the following:

1) as described above, the air bubbles are unavoidable factors of the marine environment analyzer, and are important factors affecting the numerical stability of the analyzer. The influence can be reduced by liquid path design or adding a defoaming agent and the like;

2) the influence of biological adhesion and the biological adhesion phenomenon are problems which all marine instruments need to face, an effective mode is not completely avoided at present, and in an environment analyzer, proper liquid path design and an injection mode can be selected for reduction;

3) and the influence of chemical reagent imbalance is detected by using a photometry, a plurality of reagents are used under most conditions, and according to the requirements of chemical reactions, the reagents need to be injected into the water body to be detected in a certain sequence, and the injection time interval and the reagent amount injected each time also need to meet certain requirements. For example, in some methods for detecting silicate content, 3 chemicals are used, with a ratio of 1: 1: and 2, injecting the chemical reagents into the sample respectively in sequence, wherein the injection interval of 1 reagent and 3 reagents is respectively 20 seconds and 40 seconds.

As mentioned above, most of the marine-oriented instruments adopt continuous measurement and use the flow injection method, and the chemical reaction is performed in the corresponding pipeline. In actual use, it is found that the degree of chemical reaction fluctuates to various degrees. In the same multi-parameter instrument, the fluctuation of some parameter chemical reactions is larger, and the fluctuation of some parameter chemical reactions is smaller. When a plurality of same instruments are compared, the fluctuation of the same parameters is also found, and the rules among different parameters and among the same parameters are difficult to master.

Such fluctuation and the influence of the bubbles on the instrument are combined with each other, which further causes instability of the measured value, and the generation conditions of the two influencing factors are random, which brings great difficulty to the post-processing.

In the flow injection method, there is no special module for quantifying the sample and reagent, and there is no special module for chemical reaction, and the sample and reagent are continuously flowed in the pipeline. Reagents are injected into the sample at specific points in time as required by the chemical reaction. Similarly, the sample that has completed the chemical reaction will flow continuously, and flow in one direction in the detection module, entering at one end, and flowing out at one end.

In the present stage, the injection of the reagent is performed in a time-sharing manner, namely, the injection is performed for a short time at certain specific points, and the injection is closed in other time periods. When only one reagent is required for the reaction, it is apparent that the chemical reaction proceeds only at the reagent injection point, and the other points are not changed, resulting in non-uniformity of the chemical reaction. When two or more reagents are required for the reaction, it is very easy to create a problem that the two reagents may not meet each other at all, so there may be several cases in the liquid path: there were no reagents at all, only reagent 1, only reagent 2, and both. Therefore, it is more simple to think that the chemical reaction is not complete in the final liquid path.

The electronic sampling speed is very fast, the commonly used sampling rate is dozens of ksps or even dozens of Msps, and the high sampling rate is very difficult to control when the sampling is required to be performed at the stage of the same reaction degree every time.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a flow injection method for injecting a reagent at fixed time, which can meet the stability requirement on a measured value by ensuring the stability of chemical components in a measuring optical path and adjusting the sampling time.

(II) technical scheme

The invention provides the following technical scheme:

a flow injection method for timed injection of reagents comprising the steps of:

1) adjusting the sample according to the test requirementVolume V of single injection, of

Is an integer to ensure that the signal has the same concentration fluctuation period in the detection channel at each measurement, wherein the optical path length L_{Light (es)}The length of a detection channel of liquid in a photoelectric detection module is shown, and R is the inner diameter of a pipeline after a sample and a reagent are mixed;

2) determining a single injection period according to the single injection volume V and the injection rate V of the sample,

wherein tau is_{Sample (A)}Time delay of the sample;

3) controlling the injection time of the reagent in a single sample injection period to satisfy t₀＝t_{Liquid for treating urinary tract infection}+t_{Electric power}The injection time of the reagent in each single injection period is kept consistent, so that the position of the reagent in the sample in the single injection process is kept consistent, wherein t_{Liquid for treating urinary tract infection}Is a delay caused by the fluid path, t_{Electric power}Is a controllable delay added to meet timing requirements.

Furthermore, when a plurality of reagents with the same injection amount need to be injected, the time delay parameter t of each reagent is adjusted_{Electric power}Let t be_{1 liquid}+t_{1 electricity}＝t_{Liquid 2}+t_{2 electricity}＝t_{n liquid}+t_{n electricity}To ensure that the reagents meet at the same point in time during each injection cycle so that the reagent mixing point of the mixed liquid is fixed in position within the optical detection module channel after each single injection of the sample.

Further, when a plurality of reagents with different injection amounts need to be injected, the method comprises the following steps:

1) adjusting the time delay parameter t of the reagent_{Electric power}So that the various reagents can meet at the same point in time, t, of each injection cycle_{1 liquid}+t_{1 electricity}＝t_{Liquid 2}+t_{2 electricity}＝t_{n liquid}+t_{n electricity}So that the reagent mixing point of the mixed liquid is in the optical detection module channel after each single sample injectionThe position of (2) is fixed;

2) determining the opening time t of the pump valve of each reagent according to different single injection amount of each reagent₁To t_n；

3) Adjusting the delay parameter t of the various reagents_{1 electricity}And t_{n electricity}To make

Thereby keeping the center values of the time points when the multiple reagents are injected coincident.

(III) advantageous effects

Compared with the prior art, the invention has the following beneficial effects:

by controlling the time of the reagent in the single injection process, namely controlling the position of the reagent in the sample in the single injection process, the power systems of the sample and the reagent adopt a pump valve system which can be quantified, the single sample injection process is controllable and adjustable, continuous liquid flow in the whole liquid path system is realized by continuously and repeatedly injecting samples, and the stability requirement on the measured value can be met by ensuring the stability of chemical components in a measuring optical path and adjusting the sampling time.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.