阅读说明：本技术 一种红景天甙的制备方法 (Preparation method of salidroside ) 是由 杨成东 于 2018-05-30 设计创作，主要内容包括：本发明涉及一种医药技术领域,具体涉及一种红景天甙的制备方法,其处理步骤包括：(1)将红景天根粉碎,过80目筛,乙醇回流提取；(2)高速逆流色谱仪分离；(3)浓缩干燥,即得红景天甙。本发明采用超声回流提取-高速逆流色谱分离提取红景天甙,工艺简单、提取时间短、提取率高,溶剂可全部回收,降低生产成本,可进行工业化的生产。(The invention relates to the technical field of medicines, in particular to a preparation method of salidroside, which comprises the following processing steps: (1) pulverizing radix Rhodiolae, sieving with 80 mesh sieve, and extracting with ethanol under reflux; (2) separating by a high-speed counter-current chromatograph; (3) concentrating and drying to obtain salidroside. The invention adopts ultrasonic reflux extraction-high speed counter current chromatography separation to extract the salidroside, has simple process, short extraction time and high extraction rate, can completely recover the solvent, reduces the production cost and can carry out industrialized production.)

1. The preparation method of the salidroside is characterized by comprising the following steps:

1) Pulverizing radix Rhodiolae, extracting with 60 ~ 80% ethanol at a ratio of 1:8 ~ 1:12 under reflux for 3 times (each time for 0.5 ~ 1.5.5 hr), filtering, mixing extractive solutions, and concentrating to remove ethanol to obtain concentrated solution;

2) Separating the radix Rhodiolae extract with high speed countercurrent chromatography, collecting eluate, concentrating, and drying to obtain high purity salidroside.

2. the method for preparing salidroside according to claim 1, wherein the ratio of rhodiola root powder to ethanol solvent in step 1) is 1:8 ~ 1: 12.

3. the method for preparing salidroside according to claim 1, wherein said ethanol reflux extraction time in step 1) is 0.5 ~ 1.5.5 h each time.

4. the method of claim 1, wherein the high speed countercurrent chromatography in step 3) is performed at 700 ~ 1000r/min and the mobile phase flow rate is 0.5 ~ 2.5.5 ml/min.

Technical Field

The invention belongs to the field of separation of active ingredients of natural products, and particularly relates to a method for extracting and purifying salidroside.

Background

Rhodiola rosea (Rhodiola sacra) is a perennial herb of Rhodiola of Crassulaceae, is mainly distributed in Himalayas mountain areas, northwest Asia and North America of northern hemisphere, grows in alpine, dry, anoxic, strong ultraviolet irradiation and areas with large day-night temperature difference at an altitude of 1600-4000 m, and has extremely strong environmental adaptability and vitality. Rhodiola rosea is recorded in the four medical classics and the compendium of materia medica in China. Pharmacological research shows that the main pharmacological active components of rhodiola root are salidroside, aglycone tyrosol, salidroside and the like, wherein the salidroside is a chemical component which is researched more. The research proves that the salidroside has the effects of resisting fatigue, resisting aging, regulating immunity, eliminating free radicals, enhancing memory, improving sleep, and the like, and can be used for developing functional foods.

₂The traditional preparation method has the defects of large usage amount of organic solvent, serious pollution, low extraction rate and the like, and the method adopts ethanol ultrasonic extraction-high-speed countercurrent chromatography to separate the salidroside, overcomes the defects of sample adsorption, loss, pollution, peak shape tailing and the like brought by a solid phase carrier while ensuring the extraction rate, and is an ideal technology for preparing and separating the salidroside.

Disclosure of Invention

The invention aims to provide a method for extracting salidroside with short extraction time and high yield aiming at the defects of the prior art.

The invention comprises the following steps:

Pulverizing radix Rhodiolae, extracting with 60 ~ 80% ethanol at a ratio of 1:8 ~ 1:12 under reflux for 3 times (each time for 0.5 ~ 1.5.5 hr), filtering, mixing extractive solutions, concentrating to remove ethanol to obtain concentrated solution, separating the radix Rhodiolae extract with high speed counter current chromatograph, collecting eluate, concentrating, and drying to obtain high purity salidroside.

The material-liquid ratio of the rhodiola root powder to the ethanol solvent is 1:8 ~ 1: 12.

The ultrasonic reflux extraction time of the ethanol is 0.5 ~ 1.5.5 h each time.

the parameters of the high-speed counter-current chromatograph are set as the rotating speed of 700 ~ 1000r/min and the flow rate of the mobile phase of 0.5 ~ 2.5.5 ml/min.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows: