阅读说明：本技术 一种遗体除臭消毒液 (Remains deodorizing disinfectant ) 是由 余廷 王利菊 李玉光 孙栋 余迎崟 陈涛 于 2019-09-06 设计创作，主要内容包括：本发明公开了一种遗体除臭消毒液,属于人类遗体保存的技术领域。本发明选择采用过氧化氢作为除臭消毒剂的主配方,主要是针对遗体腐败所产生的特殊臭味混合气体产生化学反应消除尸臭味,并且除臭后无残留,无有毒有害物质产生,具有广普杀菌效果。此外,本发明提供的产品性质稳定,无易燃易爆的威胁,无腐蚀性,方便购买使用。(The invention discloses a remains deodorizing disinfectant, belonging to the technical field of human remains preservation. The invention adopts hydrogen peroxide as a main formula of the deodorizing disinfectant, mainly aims at the generation of chemical reaction of special odor mixed gas generated by putrefaction of remains to eliminate the odor of the remains, has no residue after deodorization, generates no toxic and harmful substances, and has broad-spectrum sterilizing effect. In addition, the product provided by the invention has stable property, no threat of flammability and explosiveness, no corrosivity and convenient purchase and use.)

1. The deodorant disinfectant for remains is characterized by comprising the following components in percentage by mass: water: 91% -95%, hydrogen peroxide: 6-8%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.5 percent.

2. The remains deodorizing disinfectant as claimed in claim 1, wherein the mass content of each component is: water: 91% -94%, hydrogen peroxide: 6-8%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.5 percent.

3. The remains deodorizing disinfectant as claimed in claim 2, wherein the mass content of each component is: water: 91.7% -93.85%, hydrogen peroxide: 6-7.5%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

4. The remains deodorizing disinfectant as claimed in claim 3, wherein the mass content of each component is: water: 92.2% -93.35%, hydrogen peroxide: 6.5-7.3%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

5. The remains deodorizing disinfectant as claimed in claim 4, wherein the mass content of each component is: water: 92.1% -93%, hydrogen peroxide: 6.9-7.1%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

6. The remains deodorizing disinfectant as claimed in claim 1, wherein the mass content of each component is: water: 93%, hydrogen peroxide: 6.5-7.5%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

7. The remains deodorizing disinfectant as claimed in claim 1, which is prepared from the following raw materials: water, a hydrogen peroxide solution with the volume fraction of 30%, a phytic acid solution with the volume fraction of 70% and a mannitol solution with the volume fraction of 20%.

8. A method for preparing the remains deodorizing disinfectant as set forth in any one of claims 1 to 7, comprising the steps of:

(1) adding the mannitol solution with the volume fraction of 20% into the phytic acid solution with the volume fraction of 70% and uniformly stirring;

(2) Adding the solution stirred in the step (1) into water, and uniformly stirring;

(3) Adding a hydrogen peroxide solution with the volume fraction of 30% into the solution obtained in the step (2), and uniformly stirring;

(4) And (4) detecting the acid reduction degree of the prepared solution in the step (3), and adjusting the pH value of the prepared solution by using water and/or phytic acid to enable the pH value to be within the range of 3.5-4.5.

9. The method for using the deodorizing disinfectant for the corpses according to any one of claims 1 to 7, wherein the deodorizing disinfectant is uniformly shaken and then sprayed on the surface of the corpses or the space for storing the corpses.

10. The use of the body deodorizing disinfectant of any one of claims 1 to 7 for disinfecting and removing body odor.

Technical Field

The invention relates to a remains deodorizing disinfectant, belonging to the technical field of human remains preservation.

Background

After death, remains are putrefactive, and, in the process of putrefaction, odor is emitted. The gases generated by putrefaction of remains are mainly sulfur dioxide (SO)₂) Carbon dioxide (CO)₂) Hydrogen sulfide (H)₂S), ammonia (NH)₃) Methane (CH)₄) Among these gases, sulfur dioxide (SO)₂) Hydrogen sulfide (H)₂S), ammonia (NH)₃) Can cause odor. These odor causing gases not only harm the funeral service personnel's health, but also are not conducive to soothing the departed family.

At present, no special disinfectant for body deodorization is available in the funeral industry, and 84 disinfectant or Jiaomba disinfectant is generally adopted, the two disinfectants mainly use hypochlorous acid for disinfection and sterilization, the effect of eliminating body odor in the body is not great, and gas generated by body putrefaction mainly contains sulfur dioxide (SO)₂) Carbon dioxide (CO)₂) Hydrogen sulfide (H)₂S), ammonia (NH)₃) Methane (CH)₄) Among these gases, sulfur dioxide (SO) is the dominant odor causing gas₂) Hydrogen sulfide (H)₂S), ammonia (NH)₃). Hypochlorous acid used for sterilization reacts with these odor-causing gases and further generates other toxic and harmful gases, for example, HClO + SO₂+H₂O＝HCl+H₂SO₄The generated HCl has strong pungent smell and toxicity; 2HClO + H₂S＝Cl₂Gas +2H₂O + S precipitation, chlorine gas has strong pungent smell and is yellow green toxic gas; 3NH₃+HClO＝＝N₂H₄+NH₄Cl+H₂O，N₂H₄A colorless oily liquid having corrosiveness and strong reducibility, which is extremely toxic and unstable, and has a pungent odor similar to ammonia. Therefore, the disinfectant containing hypochlorous acid is used for disinfecting the putrefactive remains, so that the odor cannot be eliminated, and harmful substances are generated, and the environment and the body health of funeral workers are not improved.

In view of the fact that the disinfectant cannot achieve the aim of deodorization, at present, a deodorizing liquid is often prepared separately in funeral and interment places, the used deodorant comprises peroxyacetic acid, potassium permanganate and the like, and the peroxyacetic acid is used as the deodorant and can only be prepared at present, because the peroxyacetic acid is formed by mixing A, B two components together, A, B two components are unstable together, proper water is required to be added during preparation, and the proportion of the water is not well controlled; potassium permanganate is also more used in the prior art, and the two chemical reagents are easily explosive dangerous goods, are not beneficial to the storage of the medicines by each pair of units, and are inconvenient to use.

hydrogen peroxide is a strong oxidant, has disinfecting, antiseptic and cleaning effects, and is often used as a disinfectant and antiseptic agent. For example, CN101954105A discloses a method for removing odor of feces and urine of livestock and poultry by oxidation, which utilizes the strong oxidizing property of ferrate and the strong oxidizing property of hydroxyl radical generated by hydrogen peroxide under the catalysis of ferrate reduction product to completely and completely remove various odor components by oxidation. For another example, CN102084869A discloses a compound hydrogen peroxide disinfectant, which utilizes the characteristic that hydrogen peroxide can generate free radicals to exert a bactericidal effect.

However, the process of body deodorisation has its own specificities, including:

(1) In the process of deodorizing the dead body, the color which is not beneficial to the make-up of the dead body can not be generated, and if the method refers to the oxidation removal of the livestock and poultry manure disclosed in CN101954105AThe method for deodorizing and disinfecting the remains by utilizing the strong oxidizing property of ferrate can generate polysulfide, and dark green ferric trisulfide (Fe) can be formed on the surfaces of the remains₂S₃) The original color of the remains is changed, the remains are not beneficial to make-up, ferrate FeO₄ ^2-absorption of hydrogen sulfide H₂Chemical reactions that may occur after S gas:

2NaOH+H₂S＝NaS+2H₂O ①

2FeO₄ ^2-+3S^2-+8H₂O＝2Fe(OH)_3↓+3S↓++10OH^- ②

2FeO₄ ^2-+6S^2-+8H₂O＝Fe₂S_3↓+3S↓+16OH^- ③

S+ns＝S_N+1 ^2- ④

8FcO₄ ^2-+3S^2-+20H₂O＝8Fe(OH)_3↓+3SO₄ ^2-+16OH^- ⑤

8FeO₄ ^2-+15S^2-+20H₂O＝4Fe₂S_3↓+3SO₄ ^2-+40OH^- ⑥

(2) Measuring sulfur dioxide (SO) in the sealed space during the process of deodorizing putrefactive remains₂) At a concentration of about 1.2-8.58mg/m³Hydrogen sulfide (H)₂S) concentration of about 90-130mg/m³Ammonia (NH)₃) Has a concentration of about 150-210mg/m³(ii) a By the chemical equation SO₂+H₂O₂＝H₂SO₄、H₂O₂+H₂S＝2H₂O+S↓、2NH₃+3H₂O₂＝N_2↑+6H₂O, it can be calculated that 540.64-834.33 is consumed in the process of deodorizing putrefactive remains₂mg/m³20000mg of disinfectant containing 3% of hydrogen peroxide is required to be sprayed per cubic meter in the process of sterilizing the remains, 600mg of hydrogen peroxide is required per cubic meter, and 1m of hydrogen peroxide is required to be sprayed per cubic meter in the process of sterilizing the remains³The putrefactive odor emitted from putrefactive remains in the space is deodorized by 1140.64-1434.33 mgHydrogen oxide, and when 20000mg of the disinfection and deodorization solution is sprayed per cubic meter for achieving the purpose of disinfection and deodorization at the same time, the concentration of hydrogen peroxide in the disinfection and deodorization solution should be 5.732% -7.17%.

(3) CN102084869A discloses a compound hydrogen peroxide disinfectant, wherein the concentration of hydrogen peroxide is 8.5% -12.5%, the concentration is relatively high, if the content of hydrogen peroxide is too high, the production cost can be increased, and if the concentration of hydrogen peroxide exceeds 8%, the compound hydrogen peroxide disinfectant belongs to an easily explosive dangerous article and is not favorable for purchase and use.

Disclosure of Invention

[ problem ] to

The invention aims to provide a deodorizing disinfectant for remains, which has good and stable bacteriostatic performance, is convenient to transport and does not poison operators.

[ solution ]

The deodorizing disinfectant for remains provided by the invention comprises the following components in percentage by mass: water: 91% -95%, hydrogen peroxide: 6-8%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.5 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 91% -94%, hydrogen peroxide: 6-8%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.5 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 91.7% -93.85%, hydrogen peroxide: 6-7.5%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 91.7% -93.35%, hydrogen peroxide: 6.5-7.5%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 92.2% -93.35%, hydrogen peroxide: 6.5-7.3%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 92.1% -93%, hydrogen peroxide: 6.9-7.1%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

In one embodiment of the invention, the weight contents of the components in the remains deodorizing disinfectant are water: 93%, hydrogen peroxide: 6.5-7.5%, phytic acid: 0.1-0.5%, mannitol: 0.05 to 0.3 percent.

In one embodiment of the invention, the remains deodorizing disinfectant comprises the following components by mass: hydrogen peroxide: 6.5%, phytic acid: 0.5 percent, mannitol 0.3 percent and the balance of water.

In one embodiment of the invention, the remains deodorizing disinfectant comprises the following components by mass: hydrogen peroxide: 7%, phytic acid 0.5%, mannitol: 0.3 percent of water and the balance of water.

In one embodiment of the invention, the remains deodorizing disinfectant comprises the following components by mass: hydrogen peroxide: 7.5%, phytic acid: 0.5%, mannitol: 0.3 percent of water and the balance of water.

In one embodiment of the invention, the water (H)₂O) is preferably tertiary water (distilled water) in accordance with GB/T6682.

In one embodiment of the present invention, the purity levels of the hydrogen peroxide, the phytic acid and the mannitol for preparing the deodorizing and disinfecting solution are all laboratory analytical purity.

The invention also provides a method for preparing the remains deodorizing disinfectant, which comprises the following raw materials: water (H)₂O), 30% by volume hydrogen peroxide (H)₂O₂) Solution, 70% volume fraction phytic acid (C)₆H₁₈O₂₄P₆) Solution, 20% by volume mannitol (C)₆H₁₄O₆) A solution; the method comprises the following steps:

(1) Adding the mannitol solution with the volume fraction of 20% into the phytic acid solution with the volume fraction of 70% and uniformly stirring;

(2) Adding the stirred solution in the step (1) into water, and uniformly stirring;

(3) Adding a hydrogen peroxide solution with the volume fraction of 30% into the solution obtained in the step (2), and uniformly stirring;

(4) and (4) detecting the acid reduction degree of the prepared solution in the step (3), and adjusting the pH value of the prepared solution by using water and/or phytic acid to enable the pH value to be within the range of 3.5-4.5.

Specifically, the method for preparing the remains deodorizing disinfectant provided by the invention comprises the following steps:

(1) taking 0.65 part of phytic acid solution with the volume fraction of 70%, then taking 1.09 parts of mannitol solution with the volume fraction of 20%, adding 1.09 parts of mannitol solution with the volume fraction of 20% into 0.65 part of phytic acid solution with the volume fraction of 70%, and uniformly stirring to obtain a stabilizer;

(2) Adding the stirred stabilizer into 78.32 parts of water, and uniformly stirring;

(3) Adding 19.94 parts by volume of 30% hydrogen peroxide solution into the solution obtained in the step (2), and uniformly stirring;

(4) And (4) detecting the acid reduction degree of the solution obtained in the step (3), and adjusting the pH value of the solution by using water and/or phytic acid to enable the pH value to be 3.5-4.5.

Specifically, the method for preparing the remains deodorizing disinfectant provided by the invention comprises the following steps:

(1) Taking 0.65 part of phytic acid solution with the volume fraction of 70%, then taking 1.09 parts of mannitol solution with the volume fraction of 20%, adding 1.09 parts of mannitol solution with the volume fraction of 20% into 0.65 part of phytic acid solution with the volume fraction of 70%, and uniformly stirring to obtain the stabilizer;

(2) Adding the stirred stabilizer into 76.79 parts of water, and uniformly stirring;

(3) Adding 21.47 parts of hydrogen peroxide solution with the volume fraction of 30% into the solution in the step (2), and uniformly stirring;

(4) And (4) detecting the acid reduction degree of the solution obtained in the step (3), and adjusting the pH value of the solution by using water and/or phytic acid to enable the pH value to be 3.5-4.5.

The remains deodorizing disinfectant provided by the invention is suitable for: the surface of the corpse is disinfected and deodorized, the death site and the corpse inspection site are disinfected and deodorized, the corpse transport vehicle and the corpse treatment tool are disinfected and deodorized, the autopsy table is disinfected and deodorized, and the odor of the rotten corpse in the air of the autopsy site is eliminated. When in use, the remains deodorizing disinfectant is fully shaken up and then sprayed on the surfaces of the remains and related objects. The disinfection operator advises to wear gloves of appropriate size and length, with goggles to avoid causing allergies and other adverse reactions. When not in use, the deodorant disinfectant for remains is stored in a dark, shady and ventilated place. The remains deodorizing disinfectant provided by the invention is an external disinfectant, can not be swallowed or put into eyes, and if the remains splash into eyes or skin, the remains deodorizing disinfectant is immediately washed by clean water for at least 15 minutes and can be used for medical treatment if necessary; it needs to be stored in places which are not easy to be reached by children. The shelf life is 2 years, and the product can be used as soon as possible after being unsealed.

The basic principle of the action of the remains deodorizing disinfectant provided by the invention is as follows: the gas generated by putrefaction of remains mainly comprises sulfur dioxide (SO)₂) Carbon dioxide (CO)₂) Hydrogen sulfide (H)₂S), ammonia (NH)₃) Methane (CH)₄). While the main odor causing dead body is: sulfur dioxide (SO)₂) Hydrogen sulfide (H)₂s), ammonia (NH)₃)。(1)SO₂+H₂O₂＝H₂SO_{4 (rare)}The resulting very dilute, transparent, colorless, odorless liquid H₂SO₄。(2)H₂O₂+H₂S＝2H₂O + S ↓ resultant is H₂O and solid matter S, and no toxic and harmful gas and solid matter are generated. (3)2NH₃+3H₂O₂＝N_2↑+6H₂The product of O is N₂And H₂O, no pollution to the environment. Hydrogen peroxide is unstable after being diluted, and the hydrogen peroxide is decomposed under the conditions of light irradiation, thermal decomposition or alkali: 2H₂O₂2H under illumination or heating₂O+O_2↑(ii) a The diluted hydrogen peroxide contains a small amount of metal ions (Fe)^{2 Ten}Palpitations and tenderness^{2 to}Plasma metal ion) easily catalyzes hydrogen peroxide to generate chemical reaction and decompose into oxygen (O)₂) And water (H)₂O). In the invention, phytic acid (C) is added₆H₁₈O₂₄P₆) The phytic acid is a non-toxic organic phosphoric acid compound extracted from plants, is a complexing agent with strong chelating power in organic matters, and can complex Fe in hydrogen peroxide^{2 Ten}Palpitations and tenderness^{2 to}Plasma metal ion: (1) pb^2-+C₆H₁₈O₂₄P_6＝PbC₆H₁₆O₂₄P₆+2H⁺；(2)Fe²⁺+C₆H₁₈O₂₄P_6＝FeC₆H₁₆O₂₄P₆+2H⁺. The hydrogen peroxide is most stable at a pH value of 3.5-4.5, the phytic acid can also be used for adjusting the acidity reduction degree of the prepared deodorant, the stability of the hydrogen peroxide is improved, the storage and the transportation are convenient, mannitol is added into the phytic acid serving as a stabilizer, the hydrogen peroxide can be decomposed more slowly, the degradation of the hydrogen peroxide can be avoided after the hydrogen peroxide is used for a long time, the effective period of the prepared deodorant is more than two years, the improvement on the working environment of funeral sites is facilitated, and the body health of workers is protected.

[ advantageous effects ]

Compared with the existing remains deodorizing disinfectant product, the composition for deodorizing and disinfecting the remains provided by the invention has the following advantages:

(1) The product selects hydrogen peroxide as a main formula of the deodorizing disinfectant, mainly eliminates the odor of dead bodies by generating chemical reaction with special odor mixed gas generated by putrefaction of dead bodies, has no residue after deodorization, generates no toxic and harmful substances, and has broad-spectrum sterilizing effect.

(2) The product is detected by the disease prevention and control center of Jiangsu province, and has strong killing and inhibiting effects on pathogenic microorganisms such as staphylococcus aureus, candida albicans, escherichia coli and the like.

(3) The product is detected by a disease prevention and control center in Jiangsu province, and a one-time integral skin irritation test shows that the product has no irritation and an acute inhalation toxicity test shows that the product is of a practical non-toxic level.

(4) The product is detected by the national quality supervision and inspection center of civil blasting equipment and the chemical material testing center of Nanjing university of science and engineering, can be kept stable within the temperature range of-5 ℃ to 95 ℃, and has no threat of flammability and explosiveness and no corrosiveness.

(5) The invention adopts 6.5 to 7.5 percent of hydrogen peroxide to act on 40m³The dead body stopping space can achieve the disinfection and deodorization effects within 1-2 min, and 6.5% -7.5% of hydrogen peroxide does not belong to easily explosive dangerous goods and is convenient to purchase and use.

Detailed Description

1. And (3) reagent sources: the chemical reagent is purchased from a store, the hydrogen peroxide is purchased and used according to the requirements of an easy-to-control standard unit, and the phytic acid and the mannitol are purchased according to the common chemical reagent.

2. The method for detecting the content of the hydrogen peroxide in the remains deodorizing disinfectant or hydrogen peroxide solution comprises the following steps: titanium salt method.

Aqueous hydrogen peroxide is a colorless transparent liquid and is widely used for corrosion prevention, disinfection and sterilization. By means of H₂O₂The characteristic of generating an orange complex with titanium ions in an acid solution is that the absorbance is in direct proportion to the content of hydrogen peroxide in a sample under 430nm, and the content of the hydrogen peroxide in the sample is measured by a colorimetric method.

3. unless otherwise stated, the reagents used in the process are analytically pure, and the water is tertiary water (distilled water) specified in GB/T6682.

4. Reagent and preparation

4.1 reagents

Titanium dioxide (TiO)₂) Ammonium sulfate [ (NH)₄)₂SO₄]Concentrated sulfuric acid (H)₂SO₄) 30% hydrogen peroxide (H)₂O₂) Potassium permanganate (KMnO)₄) All are analytically pure.

4.2 preparation of Standard stock solution of Hydrogen peroxide and determination of its Hydrogen peroxide content

4.2.1 preparation of titanium solution

Taking 0.50g of titanium dioxide and 4.0g of ammonium sulfate, adding 100mL of concentrated sulfuric acid, placing in a temperature-adjustable electric heating jacket for heating, preserving heat at 150 ℃ for 15-16h, cooling, diluting with 400mL of water, and finally filtering with filter paper. And (5) the supernatant is ready for use.

4.2.2 Potassium permanganate Standard solutionPrepared according to the method specified in GB/T601.

4.2.3 Hydrogen peroxide Standard stock solutions: and (3) sucking 1mL of 30% hydrogen peroxide solution into a 100mL volumetric flask, adding water to the 100mL scale, and uniformly mixing to obtain the hydrogen peroxide standard stock solution. 20.00mL of hydrogen peroxide standard stock solution is sucked into a 250mL conical flask, 25mL of 10 percent sulfuric acid solution is added, and potassium permanganate standard solution is usedTitrate to reddish.

The concentration of hydrogen peroxide in the hydrogen peroxide standard stock solution is calculated according to the formula (1):

In the formula:

x: the concentration of hydrogen peroxide in milligrams per milliliter (mg/ml) of a hydrogen peroxide standard stock solution;

17.01: per ml potassium permanganateThe standard solution corresponds to the mass of hydrogen peroxide in milligrams (mg);

c: potassium permanganateThe concentration of the standard solution is expressed in units of moles per liter (mol/L);

v: standard solution of potassium permanganate used for titrationIn milliliters (ml).

4.2.4 Hydrogen peroxide Standard use solutions: the hydrogen peroxide standard stock was diluted to 20. mu.g/ml according to the calibration results of 4.2.3.

5. Instrumentation and equipment

An electronic balance: the sensory was 0.01 g.

A high-speed pulverizer.

Spectrophotometer 7230(430) A spectrophotometer is provided with a 5cm cuvette.

Sulfur dioxide detector, product model: EST-10-SO 2.

hydrogen sulfide gas detector, brand new liao, product model: XLA-BX-H2S.

An ammonia gas detector: mark intelligent hand-held type ammonia gas detector, the model: ammonia Gas stripper GM 8806.

6. Analysis step of hydrogen peroxide content in remains deodorizing disinfectant

6.1 sample preparation: taking 50ml of prepared remains deodorizing and disinfecting liquid sample.

6.2 specimen Curve preparation

0.00mL, 0.25mL, 0.50mL, 1.00mL, 2.50mL, 5.00mL, 7.50mL, 10.0mL of the hydrogen peroxide standard use solution (corresponding to 0. mu.g, 5. mu.g, 10. mu.g, 20. mu.g, 50. mu.g, 100. mu.g, 150. mu.g, 200. mu.g of hydrogen peroxide) was aspirated and placed in 25mL cuvettes, respectively. Adding 5.0mL of titanium solution respectively, adding water to constant volume to 25mL, shaking up, and standing for 10 min. A standard curve was drawn on absorbance at a wavelength of 430nm using a 5cm cuvette with zero point adjusted by a blank tube and with a standard series of hydrogen peroxide concentrations (. mu.g/ml).

6.3 determination of deodorant disinfectant for remains

Aspirate 10.00ml (v)₂) Putting the remains deodorizing disinfectant sample liquid into a 25mL colorimetric tube with a plug, adding 5.0mL of titanium solution, adding water, metering to 25mL, shaking up, standing for 10min, adjusting the zero point by using a 5cm cuvette and a blank tube, measuring the absorbance at the wavelength of 430nm, and simultaneously performing a reagent blank test.

Note that: if the color of the sample liquid still has interference after the absorption of the active carbon, the background color of the sample liquid is deducted, namely 5.0mL of sulfuric acid solution is used for replacing the titanium solution, and the other steps are carried out according to the method.

6.4 presentation of the results of the analysis

The hydrogen peroxide content of the sample is calculated according to the formula (2):

In the formula:

x: the unit of hydrogen peroxide content in the sample of the deodorizing and disinfecting liquid to be tested is milligram per kilogram (mg/kg)

c: hydrogen peroxide Standard application liquid Hydrogen peroxide Mass in micrograms (μ g)

v₁: the total volume of the deodorizing disinfectant sample to be tested is milliliter (mL)

m: the total mass of the sample of deodorizing and disinfecting liquid to be tested is gram (g)

v₂: the volume of the deodorizing and disinfecting liquid to be measured is measured in milliliters (mL)

The calculation results are expressed as the arithmetic mean of two independent measurements obtained under the conditions of reproducibility, and the results retain three significant figures.

6.5 precision and detection Limit

The absolute difference between two independent measurements obtained under reproducible conditions must not exceed 10% of the arithmetic mean

The detection limit of the method is 65mg/kg, and the quantification limit is 75 mg/kg.

7. Method for measuring killing rate of remains deodorizing disinfectant on escherichia coli, staphylococcus aureus and candida albicans

Escherichia coli (8099) generation 5, purchased from CGMCC; staphylococcus aureus (ATCC6538) passage 5, purchased from CGMCC; candida albicans (ATCC10231) passage 5, purchased from CGMCC.

Washing the test bacteria 24h slant culture with PBS buffer (0.03mol/L phosphate buffer) to obtain bacterial suspension with concentration of Escherichia coli bacterial suspension of 2.12 × 10⁴～2.46×10⁴cfu/mL, concentration of Staphylococcus aureus suspension 2.12X 10⁴～2.42×10⁴cfu/mL, concentration of Candida albicans suspension 1.02 × 10⁴～1.12×10⁴cfu/mL。

Setting an experimental group and a control group, wherein the experimental group takes 0.5mL of bacterial suspension and adds 0.5mL of deodorizing and disinfecting liquid, and the control group adds 0.5mL of buffer solution of LPBS into 0.5mL of bacterial suspension. The experimental group and the control group are acted at 20 ℃ for 20min, then centrifuged for 5min on a centrifuge with 8000rpm, the precipitate is collected, washed twice with 1mL PBS buffer solution, then suspended in 1mL PBS buffer solution, 0.1mL is respectively absorbed for gradient dilution, the diluted bacterial liquid is taken and coated on a plate culture medium (common nutrient agar or Sabouraud's medium), and cultured for 48h at 37 ℃ for viable colony counting. Set 3 replicates and calculate the average colony count.

The bactericidal rate is (average colony count of control group-average colony count of experimental group)/average colony count of control group × 100%

8. Method for detecting killing effect of remains deodorizing disinfectant on escherichia coli, staphylococcus aureus and candida albicans on remains surface

After the deodorizing disinfectant is sprayed for 10min, the hands of the remains are wiped by 10cm double-layer sterile gauze, then the gauze is placed in a 250mL triangular flask filled with 100mL sterile PBS buffer, and the triangular flask is shaken for 5min at the speed of 50rpm, so that microorganisms stained on the remains enter the PBS buffer. 0.1mL of the solution was aspirated from the flask for gradient dilution, and 100. mu.L of the diluted solution was applied to a plate medium (ordinary nutrient agar or Sabouraud's medium), and cultured at 37 ℃ for 48 hours to count viable colonies.

9. Method for detecting killing effect of remains deodorizing disinfectant on spores of clostridium

Clostridium, accession number JCM4926, from Biowind technical services, Inc. The culture was carried out using potato dextrose agar medium (PDA medium). The spore suspension of Clostridium is prepared according to the Disinfection technical Specification of Wang Yossen et al, and the concentration of the spore suspension is 1.32X 10⁵～1.38×10⁵cfu/mL。

An experimental group and a control group are arranged, wherein 0.5mL of spore suspension is taken and 0.5mL of deodorizing and disinfecting liquid is added in the experimental group, and 0.5mL of buffer solution of LPBS is added in the control group. The experimental group and the control group act at 20 ℃ for 20min, then are centrifuged for 5min at 8000rpm in a centrifuge, the precipitate is collected, then is washed twice by 1mL PBS buffer solution, then is suspended in 1mL PBS buffer solution, 0.1mL is respectively absorbed for gradient dilution, the diluted suspension is taken to be coated on a PDA culture medium and is cultured for 48h at 37 ℃, and the undecided spores germinate to form colonies which are counted as viable colonies. Set 3 replicates and calculate the average colony count.

The spore killing rate is (average colony number of control group-average colony number of experimental group)/average colony number of control group x 100%

10. H of space for storing remains by using remains deodorizing disinfectant₂S、SO₂、NH₃Method for detecting the effect of elimination

And after the deodorizing disinfectant is sprayed for 10min, measuring the content of the body odor gas in the air by using a sulfur dioxide detector, a hydrogen sulfide gas detector and a smart handheld ammonia gas detector respectively.