阅读说明：本技术 一种具有驱虫作用的多苞桉桉树精油及其复合菌剂和应用 (Eucalyptus multicinctus essential oil with insect expelling effect, and composite microbial agent and application thereof ) 是由 高志强 籍立民 娄倩倩 于 2019-09-20 设计创作，主要内容包括：本发明公开了一种具有驱虫作用的多苞桉桉树精油及其复合菌剂和应用,所述多苞桉桉树精油包含1,8-桉叶脑、β-甲基异丙基苯、柠檬烯、α-蒎烯、别香橙烯等组分,其中最主要的有效成分是1,8-桉叶脑。本发明公开了由多苞桉桉树精油和保藏编号为CGMCC No.18424的灰色链霉菌HL-50制备而成的复合菌剂。所述复合菌剂可以应用在畜禽养殖中,能够达到有效地驱离畜禽动物感染的多种寄生虫和抑菌的作用。本发明所述精油成分天然,品质优良,不会影响动物摄食和生长,且复合菌剂也制备简单、使用安全、无毒副作用,可以提高畜禽动物的生长性能和肠道健康,减少养殖成本,具有广阔的应用前景。(The invention discloses eucalyptus multicladus essential oil with an insect-repelling effect, a composite microbial inoculum and application thereof, wherein the eucalyptus multicladus essential oil comprises 1, 8-cineole, beta-cymene, limonene, alpha-pinene, isomyrcene and the like, and the most main effective component is 1, 8-cineole. The invention discloses a compound microbial inoculum prepared from eucalyptus multicasts essential oil and streptomyces griseus HL-50 with the preservation number of CGMCC No. 18424. The complex microbial inoculum can be applied to livestock and poultry breeding, and can achieve the effects of effectively repelling various parasites infected by livestock and poultry and inhibiting bacteria. The essential oil disclosed by the invention is natural in components and excellent in quality, does not influence the ingestion and growth of animals, is simple to prepare, safe to use and free of toxic and side effects, can improve the growth performance and intestinal health of livestock and poultry, reduces the breeding cost, and has a wide application prospect.)

1. A eucalyptus multicasts essential oil having an insect-repellent effect, comprising the following components: 1, 8-cineole, beta-cymene, limonene, alpha-pinene and terpinen-4-ol.

2. The eucalyptus multicasts essential oil having an insect-repellent effect according to claim 1, further comprising the following components: -limonene, alpha-terpineol, P-cymene, diacetone alcohol, beta-pinene, beta-myrcene, alpha-phellandrene, alpha-terpene, gamma-terpene, mucoaldehyde, carvone, phellandrene, 4-chirp limonene, eudesmene and beta-eucalyptol.

3. The method for preparing eucalyptus multicasts essential oil having insect-repellent effect according to claim 1, comprising the steps of:

(1) processing the leaves of the eucalyptus globulus by a washing tower, a dryer and a grinder to obtain crushed leaves of the eucalyptus globulus;

(2) Respectively putting the leaves of the eucalyptus globulus in the step (1) into a steam distillation kettle, a condenser and an oil-water demixer to obtain crude oil of the eucalyptus globulus essential oil;

(3) And (3) further removing moisture from the crude oil of the eucalyptus multicasts essential oil obtained in the step (2) through a fixed bed adsorption dryer, and then, removing impurity oil in a reduced pressure rectifying tower to obtain the eucalyptus multicasts essential oil.

4. The production method according to claim 3, characterized in that: the drying temperature in the step (1) is 120-200 ℃, and the crushing granularity is 10-30 meshes.

5. A complex microbial agent containing eucalyptus globulus oil according to claim 1 or 2.

6. The complex microbial inoculant according to claim 5, wherein the complex microbial inoculant further comprises a microbial content of not less than 5 x 10⁸ CFU/g of Streptomyces griseus HL-50.

7. The complex microbial inoculant according to claim 6, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No. 18424.

8. The use of the composite microbial agent containing eucalyptus multicupillata essential oil of claim 5 for preparing an insect repellent for livestock and poultry.

9. the application of the compound microbial inoculum is characterized in that the compound microbial inoculum is applied by uniformly mixing the compound microbial inoculum in an amount of 0.5-1.5 kg/ton of feed or drinking water in the edible feed or drinking water of livestock and poultry for 4-10 days, or coating the compound microbial inoculum on the surface of the livestock and poultry for 4-10 days, or spraying the compound microbial inoculum in the breeding environment of the livestock and poultry for 4-10 days.

10. The use according to claim 8, wherein the livestock and poultry animals are pigs, chickens, cattle, ducks, geese and sheep; the insect repellent is capable of inhibiting mites, lice, ticks, mosquitoes, flies, coccidia, cestodes and fasciola lata.

Technical Field

The invention belongs to the technical field of essential oil in organisms, and particularly relates to eucalyptus multiceps essential oil with an insect expelling function, a compound microbial inoculum thereof and application thereof.

Background

The essential oil is volatile aromatic substances extracted from flowers, leaves, stems, roots or fruits of plants by a steam distillation method, an extrusion method, a cold soaking method or a solvent extraction method, wherein most of the essential oil is lighter than water, and the relative density is generally 0.8-1.17. The essential oil is sensitive to heat and light, so the essential oil needs to be stored in a dark container and a shade place, and the essential oil is mainly used for maintenance products and aromatherapy at present due to the characteristics of special aroma and solubility in organic solvents, but the plant essential oil has various functions, has the effects of resisting bacteria, preventing spasm, promoting growth, promoting cell metabolism and cell regeneration, enhancing immunity, resisting oxidation and the like besides the traditional functions of flavoring, relieving and refreshing, and can even improve the quality of livestock and poultry products. One of the essential oils is wood-based essential oil extracted from wood-based plants, such as sandalwood, cedar, birch, cedar, eaglewood, eucalyptus, etc.

The eucalyptus is a general name of plants in the eucalyptus genus of the myrtaceae family, most of the plants are tall trees, the shrubs are few, and the medicinal value is rich, while the common eucalyptus essential oil in the market is mostly derived from tall trees such as eucalyptus, but the eucalyptus essential oil is light in color and luster, not thick in sense, and not lasting in smell, and finally influences the using effect of the essential oil. The eucalyptus lobular (multi-bud eucalyptus) is distributed in Australia, grows in a very arid sandy land environment, contains more eucalyptol components than general eucalyptus globulus and has higher extraction rate, and essential oil produced by the multi-bud eucalyptus has high content and good quality, is a precious medical raw material, and can be widely used in the fields of antibiosis and insect expelling.

When livestock and poultry are bred, various parasites and bacteria can be caused due to poor breeding management, poor prevention and the like, so that huge loss is directly or indirectly brought to the whole livestock and poultry breeding industry. In order to maintain the breeding benefit, the antibacterial insecticide becomes a product which is just needed for livestock breeding. However, in order to simply pursue the instant effect, the traditional medicine has the situations of heavy metal exceeding, unstable medicine dosage and the like, so that the environment is damaged, the health of livestock and poultry is damaged, the immunity of the livestock and poultry is reduced, the death event can be directly caused seriously, the production benefit is finally reduced, and the potential safety hazard is brought to consumers. Therefore, it becomes very important to prepare a natural, healthy, safe, bactericidal and anthelmintic for livestock and poultry.

Disclosure of Invention

In order to overcome the defects and shortcomings in the prior art, the invention provides eucalyptus multiceps essential oil with an insect expelling effect, a compound microbial inoculum thereof and application thereof.

in order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:

The invention provides eucalyptus multicasts essential oil with an insect-repelling effect, which is characterized by comprising the following components: 1, 8-cineole, beta-cymene, limonene, alpha-pinene, terpinen-4-ol.

further, the eucalyptus globulus oil also comprises the following components: -limonene, alpha-terpineol, P-cymene, diacetone alcohol, beta-pinene, beta-myrcene, alpha-phellandrene, alpha-terpene, gamma-terpene, mucoaldehyde, carvone, phellandrene, 4-chirp limonene, eudesmene and beta-eucalyptol.

The invention also provides a preparation method of the eucalyptus multiceps essential oil with the insect-expelling function, which comprises the following steps:

(1) Processing the leaves of the eucalyptus globulus by a washing tower, a dryer and a grinder to obtain crushed leaves of the eucalyptus globulus;

(2) Respectively putting the leaves of the eucalyptus globulus in the step (1) into a steam distillation kettle, a condenser and an oil-water demixer to obtain crude oil of the eucalyptus globulus essential oil;

(3) And (3) further removing moisture from the crude oil of the eucalyptus multicasts essential oil obtained in the step (2) through a fixed bed adsorption dryer, and then, removing impurity oil in a reduced pressure rectifying tower to obtain the eucalyptus multicasts essential oil.

Further, the drying temperature in the step (1) is 120-200 ℃, and the crushing granularity is 10-30 meshes.

The invention also provides a compound microbial inoculum containing the eucalyptus globulus essential oil.

Further, the composite microbial inoculum also comprises the microbial inoculum with the bacterial content of not less than 5 multiplied by 10⁸ CFU/g of Streptomyces griseus HL-50.

Further, the preservation number of the streptomyces griseus HL-50 is CGMCC No. 18424.

The invention also provides application of the compound microbial inoculum containing the eucalyptus globulus oil in preparing the livestock and poultry insect repellent.

further, the application method of the compound microbial inoculum comprises the following steps of uniformly mixing the compound microbial inoculum in the amount of 0.5 ~ 1.5 kg/ton of feed or drinking water in the edible feed or drinking water of livestock and poultry, and continuously feeding for 4 ~ 10 days, or coating the compound microbial inoculum on the body surface of the livestock and poultry, and continuously coating for 4 ~ 10 days, or spraying the compound microbial inoculum in the culture environment of the livestock and poultry, and continuously spraying for 4 ~ 10 days.

Further, the livestock and poultry animals are pigs, chickens, cattle, ducks, geese and sheep; the insect repellent is capable of inhibiting mites, lice, ticks, mosquitoes, flies, coccidia, cestodes and fasciola lata.

Compared with the prior art, the invention has the following advantages and beneficial effects:

(1) The invention uses the Australian multicaste eucalyptus globulus as the raw material to prepare the essential oil, the essential oil has natural components, does not influence the ingestion and the growth of animals, and has no toxicity or side effect, so the essential oil has good oil quality and effective insect expelling effect, and can continuously play a role for a long time.

(2) The compound microbial inoculum prepared from the eucalyptus globulus oil and the streptomyces griseus HL-50 can effectively repel various parasites infected by livestock and poultry, and can be taken orally and used in a mode of external coating and spraying, so that the parasite expelling is realized, the propagation of harmful flora and parasites in animal intestinal tracts and living environment can be effectively inhibited, the bacterial infection is reduced, and the growth performance of the livestock and poultry is improved and the health of the livestock and poultry is kept. In addition, the compound microbial inoculum is safe to use, does not generate any toxic or side effect on animals, is simple to prepare, is environment-friendly, has a wide application range, and can save the breeding cost, thereby having a wide application prospect.

drawings

FIG. 1 is a flow chart of a process for preparing eucalyptus multicupus essential oil according to the present invention.

FIG. 2 is a table showing the specific components of eucalyptus multicupus essential oil of the present invention.

FIG. 3 is a photograph of a colony of Streptomyces griseus HL-50 isolated according to the present invention on a Gao's synthetic No. one agar medium.

FIG. 4 shows the results of repelling insects on the body surface of a sow before and after the test by using the eucalyptus multocarpus essential oil compound microbial inoculum of the invention; fig. 4A is before the test and fig. 4B is after the test.

FIG. 5 shows the excrements of the laying hens before and after the test of the eucalyptus globulus essential oil complex microbial agent of the invention; fig. 5A is before the test and fig. 5B is after the test.

Detailed Description

The technical solution of the present invention will be described in further detail with reference to specific examples.