阅读说明：本技术 金龟子绿僵菌和氰氟虫腙杀斜纹夜蛾的组合物 (Composition for killing prodenia litura by metarhizium anisopliae and metaflumizone ) 是由 夏玉先 彭国雄 周林 于 2019-09-24 设计创作，主要内容包括：本发明属于含有微生物真菌的杀生剂技术领域,具体涉及一种杀斜纹夜蛾的组合物。所述组合物包括金龟子绿僵菌和氰氟虫腙。本发明的组合物中的活性成分金龟子绿僵菌和氰氟虫腙具有对斜纹夜蛾的显著协同增效作用。(The invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to a composition for killing prodenia litura. The composition comprises metarhizium anisopliae and metaflumizone. The active ingredients metarhizium anisopliae and metaflumizone in the composition have obvious synergistic effect on prodenia litura.)

1. The composition for killing prodenia litura is characterized in that the active ingredients comprise metarhizium anisopliae and metaflumizone.

2. The composition of claim 1, wherein the metarhizium anisopliae is metarhizium anisopliae CQMa 421.

3. The composition of claim 1 or 2, wherein the ratio of metarhizium anisopliae to metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone.

4. The composition as claimed in claim 3, wherein the ratio of metarhizium anisopliae and metaflumizone is 8 x 1000 hundred million spores: 20g of metaflumizone.

5. The preparation for killing prodenia litura is characterized in that active ingredients comprise metarhizium anisopliae and metaflumizone.

6. The preparation of claim 5, wherein the ratio of metarhizium anisopliae and metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone.

7. The preparation of claim 6, wherein the ratio of metarhizium anisopliae and metaflumizone is 8 x 1000 hundred million spores: 20g of metaflumizone.

8. The formulation according to any one of claims 5 to 7, further comprising a solvent, an emulsifier and a stabilizer.

9. The formulation of claim 8, wherein the amount of emulsifier is 5% to 10% of the total mass of the formulation.

10. the preparation of claim 8, comprising metarhizium anisopliae, metaflumizone, a solvent, an emulsifier and a stabilizer, wherein the ratio of metarhizium anisopliae to metaflumizone is 1 x 1000 million spores to 14 x 1000 million spores: 8-32g of metaflumizone, wherein the content of the metaflumizone is 8-32% of the mass of the preparation, the dosage of the emulsifier is 5-10% of the mass of the preparation, the dosage of the stabilizer is 1-3% of the mass of the preparation, and the solvent is used for supplementing 100%.

Technical Field

the invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to a composition for killing prodenia litura by metarhizium anisopliae and metaflumizone.

background

Spodoptera litura (Fabricius), also known as Neurospora litura, Spodoptera litura, Trichoplusia, Nelumbo nucifera, and the like, belong to the class Insecta, Lepidoptera, and Spodoptera litura (the temporal and spatial distribution of Spodoptera litura) in China is summarized, Wuhua-chang et al, Anhui agricultural science, volume 44, No. 9, column 2, lines 1-3 on the left column of page 142, and open day 2016, month 06, 27. The spodoptera litura larvae are 6-year old and are pseudodead. Entering a binge-eating period after 4 years, eating large-area host plant leaves when rampant and migrating to other places. Adult length 14-20mm, wing exhibition 35-46mm, body dark brown, there are white clumps on the chest back, preceding wing grey brown, the decorative pattern is many, interior horizontal line and outer horizontal line white, be wavy, there is obvious white oblique broad band line in the middle, so called prodenia litura ("peach garden prodenia litura emergence condition and prevention and cure measure", Li Jing et al, Sichuan agricultural science, 9 th 2010, page 53 left column, 1 st paragraph 1-9 line, 24 th 11.2010-24 th).

The prodenia litura is a world-wide binge eating pest which intermittently occurs, mainly takes larvae as a whole plant, is common in all parts of China, particularly has great harm and serious harm to Yangtze river basin and Huaihe river basin, has a wide host range, can harm 389 plants of 109 families, host plants comprise areca taro, strawberry, Chuzhou chrysanthemum, ligusticum wallichii, peanut, Chinese cabbage, welsh onion, soybean, tomato, sweet potato, citrus, florists chrysanthemum, medlar, lotus, black-skinned white gourd, cauliflower, Chinese yam, okra, pitaya, ginger, carnation, water spinach, pepper, plum, pear, lotus root, asparagus lettuce, cotton, cassava, alfalfa, grape, navel orange, mulberry tree, Chinese rose, tobacco, peach, watermelon, broccoli, rubber, sunflower, flax, ginkgo, camellia oleifera and the like, wherein the victimbery of cruciferous vegetables, green plants and the like are the heaviest (the countermeasure for preventing and controlling the drug resistance of the prodenia litura and the development of the prodenia litura, zhou Xiao Mei et al, insect knowledge, vol 39, No. 2, page 98, left column, lines 1-3, published 2002, month 01, 08; "research on bioactivity and field control effect of azadirachtin emulsifiable solution on prodenia litura", Dynasty and the like, apply the ecology newspaper, volume 16, 6 th in 2005, 1 st to 3 th lines in the left column of 1095, 7 months and 20 days in 2005; "artificial breeding technique of prodenia litura", yellow brilliant monarch, etc., in the silkworm industry, 32 nd volume, 3 rd phase, 2011, 1 st segment, line 1-7, left column, page 76, published 2011, 12 months and 31 days; "the summary of the spatiotemporal distribution of Spodoptera litura (Spodopteralitura) in China", Wuhuan constant et al, Anhui agricultural science, volume 44, No. 9, page 143, left column, No. 3, lines 1-6, published 2016, No. 06, No. 27).

The cluster leaf back of the prodenia litura larvae gnaw at the young age, the cluster leaf back of the prodenia litura larvae is scattered to be harmful to leaves and tender stems after the 3 rd age, the old larvae can eat fruits, the feeding property of the prodenia litura larvae is both miscellaneous and harmful to various organs, and the prodigy forms overeating at the old age (peach orchard prodenia litura occurrence condition and control measures, Li Jing, etc., Sichuan agricultural science, 9 th 2010, 1 st section of the left column at page 53, 11-14 lines, and 24 days of 2010-24 days).

Therefore, the research on the medicament capable of effectively preventing and treating prodenia litura is of great significance.

Disclosure of Invention

In view of the above, the present invention aims to provide a prodenia litura killing composition, wherein the active ingredients metarhizium anisopliae and metaflumizone in the composition have a significant synergistic effect on prodenia litura.

in order to achieve the purpose, the technical scheme of the invention is as follows:

The active ingredients of the composition for killing prodenia litura comprise metarhizium anisopliae and metaflumizone.

The prodenia litura killing refers to the ability of a substance to increase the mortality rate or inhibit the growth rate of insects of the insecta, lepidoptera, noctuidae, prodenia and prodenia species.

In the research process, the inventor unexpectedly finds that the active ingredients of the metarhizium anisopliae and the metaflumizone in the composition comprising the metarhizium anisopliae and the metaflumizone have a remarkable synergistic effect on the prodenia litura.

Further, the metarhizium anisopliae is metarhizium anisopliae CQMa 421.

Further, the proportion of the metarhizium anisopliae and the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone.

Furthermore, the proportion of metarhizium anisopliae and metaflumizone is 4 x 1000 hundred million-12 x 1000 hundred million spores: 12-28g metaflumizone.

Further, the proportion of metarhizium anisopliae and metaflumizone is 6 x 1000 hundred million-10 x 1000 hundred million spores: 16-24g metaflumizone.

Further, the proportion of metarhizium anisopliae and metaflumizone is 8 multiplied by 1000 hundred million spores: 20g of metaflumizone.

The invention also aims to protect a prodenia litura killing preparation, and the active ingredients of the prodenia litura killing preparation comprise metarhizium anisopliae and metaflumizone.

Further, the proportion of the metarhizium anisopliae and the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone.

Furthermore, the proportion of metarhizium anisopliae and metaflumizone is 4 x 1000 hundred million-12 x 1000 hundred million spores: 12-28g metaflumizone.

further, the proportion of metarhizium anisopliae and metaflumizone is 6 x 1000 hundred million-10 x 1000 hundred million spores: 16-24g metaflumizone.

Further, the proportion of metarhizium anisopliae and metaflumizone is 8 multiplied by 1000 hundred million spores: 20g of metaflumizone.

Further, the preparation is an oil suspending agent.

Further, the preparation also comprises a solvent, an emulsifier and a stabilizer.

Further, the solvent comprises vegetable oil such as rapeseed oil, sesame oil, corn oil or peanut oil.

Further, the emulsifier comprises one or more of sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol and ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine monooleate, castor oil and ethylene oxide condensate.

Further, the dosage of the emulsifier is 5-10% of the total mass of the preparation.

further, the stabilizer comprises one or more of tert-butyl p-hydroxyanisole, 2, 6-di-tert-butyl p-cresol, propyl gallate, tert-butyl hydroquinone and 2-hydroxy-4-methoxybenzophenone.

further, the dosage of the stabilizer is 1% -3% of the total mass of the preparation.

further, the preparation comprises metarhizium anisopliae, metaflumizone, a solvent, an emulsifier and a stabilizer, wherein the proportion of the metarhizium anisopliae to the metaflumizone is 1 multiplied by 1000 hundred million spores to 14 multiplied by 1000 million spores: 8-32g of metaflumizone, wherein the content of the metaflumizone is 8-32% of the mass of the preparation, the dosage of the emulsifier is 5-10% of the mass of the preparation, the dosage of the stabilizer is 1-3% of the mass of the preparation, and the solvent is used for supplementing 100%.

The invention has the beneficial effects that:

The active ingredients metarhizium anisopliae and metaflumizone in the composition have obvious synergistic effect on prodenia litura.

The composition of the present invention has excellent drug efficacy.

The preparation of the invention has good quick-acting performance.

The preparation of the invention has good persistence.

The preparation of the invention is not easy to generate drug resistance for pests.

Detailed Description

the examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.

Indoor live test combined virulence determination test:

The toxicity of the metarhizium anisopliae CQMa421 dry spore powder, metaflumizone single agent and the composition thereof (only consisting of the metarhizium anisopliae CQMa421 dry spore powder and metaflumizone, see table 1 specifically) on prodenia litura is detected, and meanwhile, the cotoxicity coefficient is calculated, and the test results are shown in table 1.

Reagent to be tested: metarhizium anisopliae CQMa421 dry spore powder, 94% wt metaflumizone raw medicine.

A test insect source: the sensitive strain is continuously fed for multiple generations in a laboratory of the genetic engineering research center of Chongqing university.

The test method comprises the following steps:

And (3) an insect soaking method, namely soaking the third-instar spodoptera litura larvae into the liquid medicine for 5 seconds after the liquid medicine is prepared, taking out the third-instar spodoptera litura larvae, sucking off excessive water, and then putting the third-instar spodoptera litura larvae into fresh feed for feeding. Three replicates of each treatment were treated, 15 replicates each.

The mortality rate of prodenia litura is investigated 72 hours after the drug administration, and data processing is carried out to compare the activity of the test drug.

Data processing:

Calculating a virulence regression equation by using POLO Plus software, wherein the calculation parameters comprise: slope b value, standard error, kaipingSquare, degree of freedom, LC₅₀Values and 95% confidence limits.

The combined virulence evaluation method comprises the following steps:

adopting a Sun Yunpei co-toxicity coefficient method:

Virulence index (TI) ═ LC of standard agents₅₀LC of test agent₅₀)×100

Actual Toxicity Index (ATI) of mixed preparation, LC for single use of A-agent₅₀LC mixed with medicament A and B₅₀)×100

The Theoretical Toxicity Index (TTI) of mixed agent is TI (A) x a + TI (B) x B

Wherein a represents the percentage of the medicament A in the mixture, and B represents the percentage of the medicament B in the mixture.

The cotoxicity coefficient CTC is (mixed agent actual virulence index ATI/mixed agent theoretical virulence index TTI) x 100;

According to the regulation of agricultural chemical examination institute of Ministry of agriculture, the judgment standard of the synergy of the mixture formula is as follows: the co-toxicity coefficient is more than 120, so that the synergistic effect is achieved; the cotoxicity coefficient is between 80 and 120, and is additive effect; the co-toxicity coefficient is less than 80, and the antagonism is obtained;

Except different medicaments, the treatment modes of the groups are the same; the results are shown in Table 1.

TABLE 1 toxicity test results of Metarrhizium anisopliae CQMa421 and metaflumizone single agent and its composition against Spodoptera litura

Medicament and proportion	LC50(mg/L)	Co-toxicity coefficient CTC
			A: metarhizium anisopliae CQMa421	64.56	-
b: metaflumizone	41.36	-
			A:B＝1:32	27.35	152.9
A:B＝4:28	19.68	220.1
			A:B＝6:24	12.66	351.9
A:B＝8:20	10.42	442.3
			A:B＝10:16	15.50	309.7
A:B＝12:12	22.58	223.3
			A:B＝14:8	42.06	127.5

Remarking: the spore content of the used destruxins CQMa421 is 200 hundred million spores/gram, the mixture ratio in the table is simplified description, destruxins CQMa: metaflumizone ═ x: y, refers to the number of metarhizium anisopliae spores x 1000 hundred million: metaflumizone weight y/gram.

As can be seen from Table 1, the ratio of the number of spores in the metarhizium anisopliae CQMa421 dry spore powder to the gram of metaflumizone is (1-14). times.1000 hundred million spores: the metaflumizone and the metaflumizone are compounded within the range of 8-32g, the cotoxicity coefficients of the two are both greater than 120, and the two show synergistic interaction; wherein in 8 x 1000 million spores: when the amount of the metaflumizone is 20g, the synergistic effect is most obvious. Therefore, the compounding of the metarhizium anisopliae CQMa421 and the metaflumizone has a remarkable synergistic effect on the prodenia litura.