阅读说明：本技术 一种复方红景天组合物及其制备方法 (Compound rhodiola rosea composition and preparation method thereof ) 是由 马家骅 韩元丰 刘青 熊亚男 向丽萍 周剑 于 2019-09-02 设计创作，主要内容包括：本发明公开了一种复方红景天组合物及其制备方法,该复方红景天组合物主要由以下重量份原料制备而成：红景天8-20份,甘草0.5-6份,乌梅0.5-6份,多肽0.1-0.5份；该复方红景天组合物仅通过三味中药的协同作用,使组合物的抗缺氧效果显著提高；同时,还通过添加多肽成分,有效的钝化了红景天提取液的苦涩味,且钝化后的鞣质能被体内酶逆分解,从而不影响药物疗效,显著提高了组合物的口感；本发明组合物的制备方法简、可靠,药效稳定性好,成本低廉,从而有利于红景天抗缺氧产品的大规模生产和广泛应用。(The invention discloses a compound rhodiola rosea composition and a preparation method thereof, wherein the compound rhodiola rosea composition is mainly prepared from the following raw materials in parts by weight: 8-20 parts of rhodiola rosea, 0.5-6 parts of liquorice, 0.5-6 parts of dark plum and 0.1-0.5 part of polypeptide; the compound rhodiola rosea composition has the advantages that the anti-hypoxia effect of the composition is obviously improved only through the synergistic effect of the three traditional Chinese medicines; meanwhile, the bitter taste of the rhodiola rosea extracting solution is effectively passivated by adding polypeptide components, and the passivated tannin can be decomposed by enzymes in vivo, so that the curative effect of the medicine is not influenced, and the taste of the composition is obviously improved; the preparation method of the composition is simple and reliable, the stability of the drug effect is good, and the cost is low, thereby being beneficial to the large-scale production and wide application of the rhodiola anti-hypoxia product.)

1. The compound rhodiola rosea composition is characterized by being mainly prepared from the following raw materials in parts by weight: 8-20 parts of liquorice, 0.5-6 parts of dark plum and 0.1-0.5 part of polypeptide.

2. The compound rhodiola rosea composition according to claim 1, wherein the molecular weight of the polypeptide is 2kda-6 kda.

3. A process for the preparation of a compound rhodiola rosea composition according to any one of claims 1 to 2, which comprises the steps of:

(1) Preparing a raw material extracting solution: pulverizing radix Rhodiolae, adding solvent, breaking cell wall with microwave, filtering, standing, and collecting upper filtrate; heating and reflux-extracting the residue for 1-3 times, and mixing filtrates to obtain radix Rhodiolae extractive solution; pulverizing Glycyrrhrizae radix and mume fructus, mixing, extracting with water for 1-3 times, and mixing extractive solutions to obtain Glycyrrhrizae radix and mume fructus extractive solution;

(2) preparing a compound rhodiola composition: mixing the rhodiola rosea extract with the liquorice and dark plum extract to obtain a mixed extract; concentrating the mixed extractive solution, adding polypeptide when concentrating to medicinal material/liquid medicine (g/ml) of 1: 1.0-3.0, and concentrating under stirring to medicinal material/liquid medicine (g/ml) of 1: 0.5-1.5 to obtain compound radix Rhodiolae composition.

4. The method according to claim 3, wherein the solvent is ethanol-K in the microwave wall-breaking treatment of step (1)₂HPO₄An aqueous two-phase extractant.

5. the method according to claim 4, wherein the alcohol-K is ethanol-K₂HPO₄In the aqueous two-phase extractant, the volume percentage of ethanol is 40-80 percent, and K is₂HPO₄the mass fraction is 10-30%.

6. The preparation method according to claim 3, wherein the microwave power is 100-300W in the microwave wall breaking treatment of step (1).

7. The preparation method according to claim 3, wherein the solvent for the heating reflux treatment of rhodiola rosea in step (1) is an aqueous ethanol solution with an ethanol volume percentage of 20-80%.

8. The preparation method according to claim 3, wherein the ratio of the medicinal material (g) to the solvent (ml) in the microwave wall-breaking treatment in step (1) is 1: 1.5-5.

9. The method according to claim 3, wherein the ratio of the crude drug (g) to the solvent (ml) in the heat reflux extraction of rhodiola rosea and the aqueous extraction of licorice and dark plum in step (1) is 1: 4-10.

10. Use of the compound rhodiola rosea composition as defined in any one of claims 1 to 2 in the preparation of anti-hypoxic drugs, foods and/or health products.

Technical Field

The invention relates to the field of traditional Chinese medicinal material medicinal preparations, in particular to a compound rhodiola rosea composition with high altitude anoxia resistance, good fatigue relieving effect and good taste and a preparation method thereof.

Background

Along with the acceleration of life rhythm and the rapid development of plateau sports, the demand of people on anti-hypoxia products is continuously increased, so that the research on the anti-hypoxia traditional Chinese medicine preparation with small adverse reaction and good anti-hypoxia effect has great research value and economic benefit under the conditions that the varieties of the existing anti-hypoxia chemical medicines are few and various adverse reactions exist. The rhodiola rosea can obviously improve the anoxia endurance of human bodies, has small side effect and is one of the best raw material medicines for preparing an anoxia resistant product. Rhodiola rosea, also called Rhodiola crenulata, is a dry root and rhizome of Rhodiola crenulata (hook.f. etthoms.) H.Ohba, which is a plant of Crassulaceae, and the rhizome of Rhodiola crenulata is commonly used as a medicine. At present, rhodiola rosea tablets, extracts, various beverages and the like are applied to the health care of workers in special environments such as divers, athletes, pilots, astronauts and the like abroad, and the research is carried out in the fields of health care products, cosmetics, medicines and the like at home. Rhodiola chemical components are complex and comprise phenol glycosides, flavonoids, coumarins, amino acids and other components, wherein salidroside (salidroside) and flavonoids of the phenol glycosides are main pharmacological active components. Modern pharmacological studies show that rhodiola rosea has good curative effects on resisting hypoxia (hypoxia), fatigue, aging, radiation, virus, microwave radiation and the like, can promote cell growth and reproduction, reduce myocardial oxygen consumption of mice and the like.

Nowadays, although various types of anti-hypoxia products related to rhodiola rosea also have certain anti-hypoxia effect, the compatibility of the components is not reasonable, so that the curative effect of the product is unclear, the anti-hypoxia drug effect is different, and the product cost is higher, thereby seriously limiting the large-scale production and wide application of the rhodiola rosea anti-hypoxia product. Meanwhile, the rhodiola rosea extract contains a large amount of tannin components which are easily combined with protein to form insoluble precipitates, so that the product has astringency and strong astringent taste, has poor mouthfeel and is unacceptable to general consumers, and after the rhodiola rosea tannin (such as patent publication No. CN103977056A) is removed, the drug effect is obviously reduced, and the development and popularization of the product are greatly influenced.

Disclosure of Invention

The invention mainly aims to overcome the defects of poor anti-hypoxia effect and poor taste of the existing rhodiola rosea composition product, and provides a compound rhodiola rosea composition and a preparation method thereof. The compound rhodiola rosea composition has the advantages that the anti-hypoxia effect of the composition is obviously improved only through the synergistic effect of the three traditional Chinese medicines; meanwhile, the bitter taste of the rhodiola rosea extract is effectively passivated by adding polypeptide components, and the passivated tannin can be decomposed by enzymes in vivo, so that the curative effect of the medicine is not influenced, and the medication compliance of a patient is obviously improved.

In order to realize the aim, the invention provides a compound rhodiola rosea composition which is mainly prepared from the following raw material medicines in parts by weight: 8-20 parts of rhodiola rosea, 0.5-6 parts of liquorice, 0.5-6 parts of dark plum and 0.1-0.5 part of polypeptide.

The compound rhodiola composition disclosed by the invention takes rhodiola, liquorice and dark plum as main raw materials, adopts a method of combining and researching modern medicine and traditional Chinese herbal medicine, innovatively provides a traditional Chinese medicine composition formula for promoting the coordination of various active ingredients, can exert an excellent anti-hypoxia effect and has a good taste. In the composition, the rhodiola rosea has the effects of tonifying qi and activating blood, and promoting blood circulation and relieving asthma, and is a monarch drug; the liquorice has the functions of tonifying qi and spleen, moistening lung and relieving cough, is matched with the rhodiola root, can supplement qi and relieve asthma, and is used as a ministerial drug; wu Mei astringes intestines, astringes lung, promotes fluid production, relieves ascaris, and excels at promoting fluid production to quench thirst, which is an adjuvant drug; the three medicines are matched and synergized to treat the symptoms of heart-qi deficiency, palpitation, intermittent pulse, spleen-stomach qi deficiency, lassitude and hypodynamia and the like. Wherein, the acute anoxia is caused by cerebral edema and pulmonary edema due to insufficient oxygen supply, and is mainly manifested by symptoms of chest distress, short breath, dry mouth and tongue, light headedness and the like; according to the syndrome differentiation theory of traditional Chinese medicine, acute anoxia is heart-lung qi deficiency, and the three traditional Chinese medicines in the compound rhodiola root composition bring out the best in each other in terms of efficacy and have a synergistic effect. Meanwhile, in order to further correct the influence of astringency brought by a large amount of tannin in the rhodiola rosea extract without reducing the drug effect, the composition of the invention uses a polypeptide component, combines the polypeptide and the tannin by utilizing the action principle of an acylamino group and a phenolic hydroxyl group to generate fine substances suspended in the extract, and the fine substances can be concentrated along with the extract. Clinical test results show that the compound rhodiola rosea composition has good antioxidant effect and good taste.

preferably, the compound rhodiola rosea composition is mainly prepared from the following raw materials in parts by weight: 8-20 parts of rhodiola rosea, 0.5-3 parts of liquorice, 0.5-3 parts of dark plum and 0.05-0.4 part of polypeptide; through optimization, the synergistic interaction among the raw material medicines is better, the passivation effect of the polypeptide on tannin is better, and the anti-hypoxia effect and the mouthfeel of the compound rhodiola rosea composition are better.

More preferably, the compound rhodiola rosea composition is mainly prepared from the following raw materials in parts by weight: 10 parts of rhodiola rosea, 1 part of liquorice, 1 part of dark plum and 0.13 part of polypeptide; through optimization, the synergistic effect among the raw material medicines is the best, the passivation effect of the polypeptide on tannin is the best, and the anti-hypoxia effect and the mouthfeel of the compound rhodiola rosea composition are the best.

Wherein the polypeptide is a product formed by proteolysis; researches show that the molecular weight of the polypeptide is too small, and the passivation effect on the bitter and astringent taste of the tannin is obviously reduced after the polypeptide is combined with the tannin; the polypeptide has overlarge molecular weight, and can form large-particle precipitates after being combined with tannin, so that the polypeptide is not beneficial to production and reverse decomposition in a human body, and the drug effect of the composition is seriously influenced; preferably, the polypeptide has a molecular weight of 2kda-6 kda; the optimized polypeptide has good passivation effect on tannin, is easy to reverse decompose, and does not influence the efficacy of the composition. Most preferably, the polypeptide has a molecular weight of 4 kda.

In order to achieve the above object, the present invention further provides a preparation method of a compound rhodiola root composition, comprising the following steps:

(1) Preparing a raw material extracting solution: pulverizing radix Rhodiolae, adding solvent, breaking cell wall with microwave, filtering, standing, and collecting upper filtrate; heating and reflux-extracting the residue for 1-3 times, and mixing filtrates to obtain radix Rhodiolae extractive solution; pulverizing Glycyrrhrizae radix and mume fructus, mixing, extracting with water for 1-3 times, and mixing extractive solutions to obtain Glycyrrhrizae radix and mume fructus extractive solution;

(2) Preparing a compound rhodiola composition: mixing the rhodiola rosea extract with the liquorice and dark plum extract to obtain a mixed extract; concentrating the mixed extractive solution, adding polypeptide when concentrating to medicinal material/liquid medicine (g/ml) of 1: 1.0-3.0, and concentrating under stirring to medicinal material/liquid medicine (g/ml) of 1: 0.5-1.5 to obtain compound radix Rhodiolae composition.

the preparation method of the compound rhodiola root composition comprises the steps of firstly performing microwave wall breaking treatment on rhodiola root, crushing cells by using the action of a microwave electromagnetic field, diffusing effective active ingredients in the cells into a solvent to be dissolved, accelerating the diffusion of the extracted ingredients from the interior of a substance to the interface of the solvent, and adopting ethanol-K according to the benzene ring structure contained in the molecular structure of the rhodiola root₂HPO₄The double-aqueous-phase extractant is used as a wall-breaking solvent, under the extraction action of a double-aqueous-phase system, the salidroside is effectively dissolved and enriched in the upper-layer liquid ethanol solution, so that the extraction rate of the salidroside is greatly increased, the effective components in the rhodiola can be more quickly and effectively dissolved under the combined action of the double-aqueous-phase extractant and microwaves, the transfer rate of the effective components in the rhodiola can be remarkably improved, and the extraction time can be shortened; then carrying out reflux extraction on the filter residue, and taking an ethanol water solution as a solvent, so that residual effective components in the filter residue can be extracted to the maximum extent, excessive water-soluble impurities can be prevented from entering the solvent, and the extraction rate of the effective components is reduced on the contrary; in addition, the polypeptide powder with proper molecular weight is added into the extracting solution, the bitter taste of the extracting solution is effectively improved by combining the polypeptide powder with tannin in the extracting solution, and the polypeptide powder can be prepared into a preparation after being concentrated along with the extracting solution, so that the problem of the bitter taste of the rhodiola rosea extracting solution is effectively solved, the content of active ingredients in the preparation is ensured, and the curative effect of the preparation is not influenced; the preparation method of the invention is simple and reliable,and the stability is good, thereby being beneficial to the large-scale industrial production of the compound rhodiola root composition.

Wherein, in the step (1), the crushing fineness of the raw materials is preferably 5-20 meshes; the optimized raw materials have the advantages of high crushing fineness, high extraction speed and high transfer rate.

Wherein, the preferable microwave wall breaking solvent in the step (1) is ethanol-K₂HPO₄An aqueous two-phase extractant; more preferably, ethanol-K₂HPO₄In the aqueous two-phase extractant, the volume percentage of ethanol is 40-80 percent, and K is₂HPO₄The mass fraction is 10-30%; the preferable solvent has better solubility to effective components such as salidroside.

Wherein, preferably, the heating reflux solvent of the rhodiola rosea filter residue in the step (1) is an ethanol water solution with the ethanol volume percentage of 20-80 percent; the preferable solvent has better solubility to effective components such as salidroside.

wherein, preferably, in the step (1) of the microwave wall breaking treatment of the rhodiola rosea, the microwave power is 100-300W. The preferable microwave power has higher transfer rate, higher speed and less impurities to the effective components in the rhodiola rosea.

Wherein, preferably, in the microwave wall breaking treatment in the step (1), the ratio of the medicinal material (g) to the solvent (ml) is 1: 1.5-5; the preferable ratio of the medicinal materials to the solvent has better extraction effect and higher extraction efficiency on the active ingredients. Preferably, the microwave wall breaking treatment time is 5-20 min; the optimized microwave wall breaking treatment time has good extraction effect on effective components, and can reduce impurities from entering a solvent.

Wherein, preferably, in the heating reflux extraction of the rhodiola root and the water extraction of the liquorice and the dark plum in the step (1), the ratio of the medicinal material (g) to the solvent (ml) is 1: 4-10; the preferable ratio of the medicinal materials to the solvent has better extraction effect on the active ingredients. Preferably, the extraction time is 30-180 min/time; the extraction efficiency is higher due to the preferable extraction time.

the compound rhodiola composition is applied to preparing anti-hypoxic drugs, foods and/or health care products.

The compound rhodiola root composition can be added with conventional auxiliary materials or carriers according to a conventional process to prepare one or more products of decoction, beverage, oral liquid, mixture, granules, capsules, tablets, syrup, granules or pills.

Compared with the prior art, the invention has the beneficial effects that:

1. The three traditional Chinese medicines in the compound rhodiola root composition bring out the best in the drug effect, have the synergistic interaction effect, and obviously improve the anti-hypoxic drug effect (the effective rate is not less than 90%) of the rhodiola root product.

2. Different from the prior art of directly removing tannin by precipitation, the invention utilizes the combination of micromolecular polypeptide and tannin, not only obviously improves the taste of the rhodiola root preparation, but also does not influence the content of effective components in the preparation, thereby ensuring the curative effect of the preparation.

3. In the preparation method of the compound rhodiola rosea composition, the wall breaking treatment is firstly carried out on the rhodiola rosea by adopting microwave and double aqueous phases, then the reflux extraction is carried out, the solvent components are adjusted according to the requirements, the content of the effective components in the rhodiola rosea is extracted in a larger quantity, the extraction time is shortened, the stability is good, and the large-scale industrial production of the compound rhodiola rosea composition is facilitated.

4. The preparation method of the compound rhodiola rosea composition is simple and reliable, has good stability, can reduce the production cost of the compound rhodiola rosea composition, improves the taste and the drug effect, and is beneficial to the large-scale production and application of the compound rhodiola rosea composition.

Detailed Description

The foregoing summary of the invention is described in further detail below with reference to specific embodiments. It should not be understood that the scope of the above-described subject matter of the present invention is limited to the following examples. Various substitutions and alterations can be made without departing from the technical idea of the invention as described above, according to the common technical knowledge and conventional means in the field, and the scope of the invention is covered.

The rhodiola rosea, the liquorice and the dark plum used in the following listed examples are medicinal materials which have a storage period of less than half a year and meet the requirements of 'Chinese pharmacopoeia' 2015 edition, and can be obtained from commercial approaches.