阅读说明：本技术 一种快速分离蚊虫蛹的方法 (Method for quickly separating mosquito pupae ) 是由 孟翔 戴建青 陈大嵩 欧阳革成 于 2019-07-31 设计创作，主要内容包括：本发明公开了一种快速分离蚊虫蛹的方法。它是将蚊子的幼虫和蛹的混合物倒入到冰水中,蛹漂浮于水面,幼虫沉于冰水中,分别捞起上层的蛹和下层的幼虫,得到分离的蛹和幼虫。本发明人发现,当蚊子的幼虫和蛹放于冰水中,幼虫由于被冰冻一瞬间会自动下沉,而蛹则会漂于水面,当捞出蛹后,冰水的温度回升至常温(25℃)的时候,幼虫又会自动上浮上来。因此,本发明能够有效的分离蚊子的幼虫和蛹,提高蚊子幼虫和蛹的分离效率,省时省力,不损伤蛹。(the invention discloses a method for quickly separating mosquito pupae. The method comprises the steps of pouring a mixture of mosquito larvae and pupae into ice water, floating the pupae on the water surface, sinking the larvae into the ice water, and respectively fishing out the upper pupae and the lower pupae to obtain separated pupae and larvae. The inventor finds that when larvae and pupae of mosquitoes are placed in ice water, the larvae automatically sink at the moment of being frozen, the pupae float on the water surface, and when the temperature of the ice water is raised to normal temperature (25 ℃) after the pupae is fished out, the larvae automatically float upwards. Therefore, the mosquito larva and pupa separating device can effectively separate the mosquito larvae and pupae, improves the separation efficiency of the mosquito larvae and pupae, saves time and labor, and does not damage the pupae.)

1. A method for rapidly separating mosquito pupae is characterized by comprising the following steps:

Pouring the mixture of mosquito larva and pupa into ice water, floating pupa on water surface, depositing larva in ice water, and respectively fishing out upper pupa and lower pupa to obtain separated pupa and larva.

2. the method of claim 1, wherein said mixture of mosquito larvae and pupae is obtained by: pouring culture medium or culture solution containing mosquito larva and pupa into screen mesh, filtering with water, and removing the medium to obtain mosquito larva and pupa mixture.

3. The method of claim 2, wherein the screen is a 20 mesh screen.

4. The method of claim 1, wherein the culture medium or culture solution containing the larvae and pupae of the mosquito is poured into a sieve, filtered with water, and the medium or culture solution is removed to obtain a mixture of the larvae and pupae of the mosquito, the mixture of the larvae and pupae of the mosquito is poured into ice water, the pupae float on the water surface, the larvae sink in the ice water, then the pupae are collected with a filtering spoon with a sieve, and the larvae in the lower layer are collected to obtain separated pupae and larvae.

Technical Field

the invention belongs to the field of mosquito control, and particularly relates to a method for quickly separating mosquito pupae.

Background art:

The major harm to mosquitoes is the transmission of disease. Mosquito-transmitted diseases have been studied as many as 80. Killing mosquitoes is an effective way to prevent and reduce diseases. In the experiment, a large amount of mosquitoes are needed to carry out researches on attractants, repellent agents, sterility technologies and the like. Therefore, a large amount of mosquito pupae and later adults need to be obtained for experimental verification.

The invention content is as follows:

The invention aims to provide a method for quickly separating mosquito pupae.

The invention discloses a method for quickly separating mosquito pupae, which comprises the following steps:

Pouring the mixture of mosquito larva and pupa into ice water, floating pupa on water surface, depositing larva in ice water, and respectively fishing out upper pupa and lower pupa to obtain separated pupa and larva.

The collected pupae can be used for pupal stage tests or experimental needs of adult culture.

Preferably, said mixture of mosquito larvae and pupae is obtained by: pouring culture medium or culture solution containing mosquito larva and pupa into screen mesh, filtering with water, and removing the medium to obtain mosquito larva and pupa mixture.

The screen is a 20-mesh screen.

Preferably, the culture medium or culture solution containing the larvae and pupae of the mosquito is poured into a screen, filtered with water, the medium or culture solution is removed to obtain a mixture of the larvae and pupae of the mosquito, the mixture of the larvae and pupae of the mosquito is poured into ice water, the pupae floats on the water surface, the larvae sink in the ice water, then the pupae is collected by a filter spoon with a screen, and the larvae on the lower layer are collected to obtain separated pupae and larvae.

the inventor finds that when larvae and pupae of mosquitoes are placed in ice water, the larvae automatically sink at the moment of being frozen, the pupae float on the water surface, and when the temperature of the ice water is raised to normal temperature (25 ℃) after the pupae is fished out, the larvae automatically float upwards.

Therefore, the mosquito larva and pupa separating device can effectively separate the mosquito larvae and pupae, improves the separation efficiency of the mosquito larvae and pupae, saves time and labor, and does not damage the pupae.

description of the drawings:

FIG. 1 is a graph showing the effect of separation of mosquito larvae and pupae.

The specific implementation mode is as follows:

The following examples are further illustrative of the present invention and are not intended to be limiting thereof.