阅读说明：本技术 一种利用三七渣混菌发酵生产灵芝菌质的方法 (method for producing ganoderma lucidum mycoplasm by using mixed fermentation of pseudo-ginseng residue ) 是由 谭显东 卢上飞 陈楠 魏琨 羊依金 于 2019-09-16 设计创作，主要内容包括：本发明公开了一种利用三七渣混菌发酵生产灵芝菌质的方法。本发明是这样实现的：(1)对三七渣进行干燥、粉碎、筛分预处理；(2)将经过预处理的三七渣、枸杞多糖、水和氮源按一定比例混合均匀；(3)将混合均匀的物料进行蒸汽灭菌处理；(4)在灭菌后的物料上接种康宁木霉和灵芝进行混菌固态发酵；(5)发酵培养物经干燥后就得到灵芝菌质。采用本发明所述方法,可以将三七渣转化为具有独特性味功能的灵芝菌质。该方法将三七渣进行资源化利用,变废为宝,不仅减少了其对环境造成的污染,还可以获得一种具有独特性味功能的灵芝菌质。(The invention discloses a method for producing ganoderma lucidum mycoplasm by using mixed fermentation of pseudo-ginseng residue. The invention is realized by the following steps: (1) drying, crushing and screening the pseudo-ginseng residue; (2) uniformly mixing the pretreated pseudo-ginseng residue, lycium barbarum polysaccharide, water and a nitrogen source according to a certain proportion; (3) carrying out steam sterilization treatment on the uniformly mixed materials; (4) inoculating trichoderma koningii and lucid ganoderma on the sterilized material to perform mixed bacteria solid state fermentation; (5) drying the fermentation culture to obtain the ganoderma lucidum mycoplasm. By adopting the method, the pseudo-ginseng residue can be converted into the ganoderma lucidum mycoplasm with unique flavor function. The method utilizes Notoginseng radix residue as resource, changes waste into valuable, reduces environmental pollution, and can obtain Ganoderma mycoplasm with unique flavor.)

1. A method for producing ganoderma lucidum mycoplasm by using pseudo-ginseng residue as a raw material through a mixed fungus solid state fermentation process is characterized by comprising the following specific process steps:

(1) pretreatment of pseudo-ginseng residue

Drying wet pseudo-ginseng residue, pulverizing, sieving, and selecting pseudo-ginseng residue with particle size of 0.2-0.4mm for subsequent process;

(2) compounding and compounding

uniformly mixing the pretreated pseudo-ginseng residue, a proper amount of nitrogen source, lycium barbarum polysaccharide and water, wherein the specific batching mode is as follows:

100 parts of pretreated pseudo-ginseng residue, 1-6 parts of nitrogen source and 0.05-0.1 part of lycium barbarum polysaccharide, wherein the mass ratio of the solid material to the water is 1:1.5-1: 2.5;

When in burdening, the nitrogen source and the lycium barbarum polysaccharide are added into water and uniformly mixed, and then the prepared solution is uniformly mixed with the pseudo-ginseng residue;

(3) Sterilization

Sterilizing the mixture by adopting a high-pressure steam sterilization method: steam pressure is 1atm, temperature is 121 ℃, and time is at least 30 minutes;

(4) Fermentation of

and (3) performing mixed fermentation on the mixture after the sterilization treatment, inoculating strains according to the inoculation amount of 10-20%, wherein the fermentation conditions are as follows: the pressure is normal pressure, the temperature is 25-30 ℃, and the fermentation period is 10-18 days;

(5) Drying

Drying the fermentation culture to obtain the ganoderma lucidum mycoplasm product.

2. the method according to claim 1, wherein the nitrogen source used in the compounding is one or more selected from yeast powder, peptone, ammonium nitrate and ammonium sulfate.

3. The method according to claim 1, wherein the fermentation is carried out using a strain comprising at least one of Ganoderma lucidum and Trichoderma koningii.

4. The process according to claim 1, wherein the Trichoderma koningii used in the fermentation is Trichoderma koningii (Trichoderma koningii) (2:)Trichoderma koningii3.3743), Trichoderma koningii (Trichoderma koningii), (C1)Trichoderma koningii3.2774), Trichoderma koningii (C.) (Trichoderma koningii3.17875) is used.

5. the method according to claim 1, wherein the Ganoderma lucidum used in the fermentation is Ganoderma lucidum: (A. RTM.), (B. RTM.), (CGanoderma lucidum5.00535), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00534), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00653), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00533), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00706) is used.

6. The ganoderma lucidum mycoplasm obtained by the method according to any one of claims 1 to 5, wherein the product contains abundant components such as protein, amino acid, alkaloid, various trace elements, notoginsenoside, notoginseng polysaccharide, notoginseng flavone B, dencichine, ganoderma lucidum polysaccharide, ganoderma lucidum triterpene and the like, and has the effects of regulating immunity, resisting tumors, resisting cancers, resisting liver injury, relieving cough and asthma, resisting aging and the like.

Technical Field

The invention belongs to the field of industrial waste recycling, and relates to a method for producing ganoderma lucidum mycoplasm by utilizing mixed fungus solid fermentation of pseudo-ginseng residue.

Background

With the rapid development of the traditional Chinese medicine industry in China, the waste amount of the traditional Chinese medicine residues in various large traditional Chinese medicine pharmaceutical factories in China is increasing day by day. The traditional treatment methods such as incineration, landfill and fixed area stacking are often adopted for the treatment of the traditional Chinese medicine residues, which not only needs to invest a large amount of capital, but also causes resource waste and serious environmental pollution. Therefore, how to reasonably dispose and utilize the herb residue is an important problem which cannot be avoided in the process of modernizing the Chinese herbs. At present, the approaches for recycling Chinese herb residues mainly comprise fermentation production of protein feed, use as a feed additive, preparation of organic fertilizer, preparation of active carbon, pyrolysis for gas production and the like.

Pseudo-ginseng is a traditional rare Chinese medicinal material in China, the root and stem of the pseudo-ginseng can be used as a medicine, the main medicinal component of the pseudo-ginseng is saponin, and pseudo-ginseng slag after the effective components are extracted is usually treated as waste. The panax notoginseng residue after the total saponins are extracted still contains nutrient substances and medicinal components such as starch, cellulose, protein, amino acid, alkaloid, panax notoginseng polysaccharide, dencichine, panax notoginseng flavone B and various trace elements, and the like, and the panax notoginseng residue is used as a fermentation substrate for further development and utilization, so that waste can be changed into valuable, and the utilization efficiency of resources can be improved. The Chinese invention patent with the application number of 201010232573.9 discloses 'a white spirit prepared by fermenting and distilling pseudo-ginseng residue and a method thereof'; the Chinese invention patent with the application number of 201110119291.2 discloses a method for producing protein feed by fermenting pseudo-ginseng residue; the Chinese invention patent with the application number of 201210196021.6 discloses a method for producing a trichoderma biocontrol agent by utilizing the fermentation of pseudo-ginseng residue; the Chinese patent with application number 201310437762.3 discloses a method for producing ganoderma lucidum notoginseng mycoplasm by fermenting notoginseng residues, and the Chinese patent with application number 201310437721.4 discloses a method for producing medicinal mycoplasm by mixed fermentation of notoginseng residues. The traditional medicinal fungus is not only an ideal health food, but also has the effect of 'curing diseases by medicine', has various effects in preventing and treating diseases, and has the functions of strengthening body resistance and consolidating constitution. Considering that trichoderma koningii has strong saccharification capacity and can promote conversion of starch substances in pseudo-ginseng residues, and lycium barbarum polysaccharide can promote synthesis of ganoderma lucidum triterpenes, the invention provides a principle and a method for applying mixed fermentation, and the trichoderma koningii and ganoderma lucidum are used for mixed solid fermentation of the pseudo-ginseng residues, and the lycium barbarum polysaccharide is added into a fermentation culture medium, so that a medicinal mycoplasm with unique property and taste functions can be obtained.

Ganoderma lucidum is called Mesona chinensis Benth and Rumex japonicus Benth, and is Basidiomycetes and Polyporaceae, and contains polysaccharide, triterpene, protein, nucleoside, and multiple trace elements such as zinc, molybdenum, iron, boron, copper, germanium, etc. as effective components. Ganoderma has effects in regulating immunity, resisting tumor, resisting liver injury, lowering blood sugar, resisting aging, and improving memory. Trichoderma koningii can produce various enzymes in the growing process, such as amylase, saccharifying enzyme, cellulase, phytase, pectinase and the like. Wherein amylase, saccharifying enzyme, etc. can hydrolyze starch to form low molecular substances such as dextrin, oligosaccharide and glucose; the protease can decompose protein into small molecular compounds such as polypeptide and amino acid; cellulases can promote the decomposition of cellulose. Through the fermentation of trichoderma koningii or with glossy ganoderma, starch, cellulose, saccharide, protein and other matters in notoginseng dregs may be utilized to produce new secondary metabolite, such as glossy ganoderma polysaccharide, glossy ganoderma triterpene, ergosterol, etc. Because the trichoderma koningii and the lucid ganoderma have good mutualism relationship, the trichoderma koningii can also promote the growth of the lucid ganoderma and the generation of metabolites in the mixed fermentation process. In addition, a proper amount of lycium barbarum polysaccharide is added into the fermentation substrate pseudo-ginseng residue, so that the growth of ganoderma lucidum mycelia and the synthesis of ganoderma lucidum triterpenes can be promoted.

in a word, through the combined action of the trichoderma koningii, the ganoderma lucidum and the lycium barbarum polysaccharide, the content of non-medicinal components in the pseudo-ginseng residue can be reduced, a large number of new active components are generated, and the content of ganoderma lucidum triterpene in a fermentation culture is improved, so that the ganoderma lucidum mycoplasm with unique flavor function is formed.

Disclosure of Invention

The invention aims to provide a method for producing ganoderma lucidum mycoplasm by using mixed fermentation of pseudo-ginseng residue. The invention relates to a method for producing ganoderma lucidum mycoplasm by utilizing mixed fermentation of pseudo-ginseng residue, which sequentially comprises the following process steps:

1. pretreatment of pseudo-ginseng residue

drying wet pseudo-ginseng residue, pulverizing, sieving, and selecting pseudo-ginseng residue with particle size of 0.2-0.4mm for subsequent process;

2. Compounding and compounding

Uniformly mixing the pretreated pseudo-ginseng residue, a proper amount of nitrogen source, lycium barbarum polysaccharide and water, wherein the specific batching mode is as follows:

100 parts of pretreated pseudo-ginseng residue, 1-6 parts of nitrogen source and 0.05-0.1 part of lycium barbarum polysaccharide, wherein the mass ratio of the solid material to the water is 1:1.5-1: 2.5;

When in burdening, the nitrogen source and the lycium barbarum polysaccharide are added into water and uniformly mixed, and then the prepared solution is uniformly mixed with the pseudo-ginseng residue;

3. Sterilization

Sterilizing the mixture by adopting a high-pressure steam sterilization method: steam pressure is 1atm, temperature is 121 ℃, and time is at least 30 minutes;

4. Fermentation of

And (3) performing mixed fermentation on the mixture after the sterilization treatment, inoculating strains according to the inoculation amount of 10-20%, wherein the fermentation conditions are as follows: the pressure is normal pressure, the temperature is 25-30 ℃, and the fermentation period is 10-18 days;

5. Drying

Drying the fermentation culture to obtain the ganoderma lucidum mycoplasm product.

In the method, the nitrogen source used in the material preparation is one or more of yeast powder, peptone, ammonium nitrate and ammonium sulfate.

in the method, the strains used in the step 4 at least comprise a ganoderma lucidum and a trichoderma koningii.

in the method, the trichoderma koningii used in the step 4 is trichoderma koningii (f) (trichoderma koningii)Trichoderma koningii3.3743), Trichoderma koningii (Trichoderma koningii), (C1)Trichoderma koningii3.2774), Trichoderma koningii (C.) (Trichoderma koningii3.17875) is used.

In the method, the ganoderma lucidum used in the step 4 is ganoderma lucidum: (a)Ganoderma lucidum5.00535), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00534), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00653), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganoderma lucidum5.00533), Ganoderma (Ganoderma lucidum (Fr.) karst.)Ganodermalucidum5.00706) is used.

The invention has the following beneficial effects:

(1) By adopting the method, the pseudo-ginseng residue can be converted into the ganoderma lucidum mycoplasm, so that the pollution to the environment is reduced, a novel ganoderma lucidum mycoplasm is provided for the traditional Chinese medicine industry, and the ganoderma lucidum mycoplasm has multiple effects of regulating immunity, resisting tumors, resisting cancers, resisting liver injury, relieving cough and asthma, resisting aging and the like. The invention is beneficial to the production enterprises to realize energy conservation, emission reduction and clean production.

(2) Compared with the prior art, the content of ganoderma lucidum polysaccharide and ganoderma lucidum triterpene in the ganoderma lucidum mycoplasm prepared by the method is obviously improved.

Detailed Description