阅读说明：本技术 一种含有中药成分的斜纹夜蛾人工饲料及其制备方法 (Spodoptera litura artificial feed containing traditional Chinese medicine components and preparation method thereof ) 是由 王占娣 杨海林 张立猛 史云东 贾琳 仲一卉 周文宾 王美佳 于 2019-10-29 设计创作，主要内容包括：一种含有中药成分的斜纹夜蛾专用饲料,原料为：麦胚、玉米粉、番茄酱、酵母粉、蒸馏水等及中药醇提取液。其制备方法：a、制备中药醇提取液；b、将麦胚、玉米粉、番茄酱、酵母粉等混匀后加蒸馏水,搅拌,灭菌30,冷却,为物料1；c、将原料中的其它成分与中药醇提取液混匀,加蒸馏水搅拌,为物料2；d、将两物料混匀,为块状的一种含有中药成分的斜纹夜蛾专用饲料；4℃保存。本发明的配方科学,成分全面,营养价值高,制作成本低,使用方便,安全可靠,无毒副作用,采用本发明的饲料饲养的幼虫的营养充足、生长迅速,虫体健康,可提高斜纹夜蛾幼虫产出率,是一种新型的含有中药成分的斜纹夜蛾人工饲料及其制备方法。(A prodenia litura special feed containing traditional Chinese medicine components comprises the following raw materials: wheat germ, corn flour, tomato sauce, yeast powder, distilled water and the like, and traditional Chinese medicine alcohol extract. The preparation method comprises the following steps: a. preparing traditional Chinese medicine alcohol extract; b. uniformly mixing wheat germ, corn flour, tomato sauce, yeast powder and the like, adding distilled water, stirring, sterilizing for 30 percent, and cooling to obtain a material 1; c. mixing other components of the raw materials with the Chinese medicinal alcohol extractive solution, adding distilled water, and stirring to obtain material 2; d. mixing the two materials uniformly to obtain a block-shaped feed containing traditional Chinese medicine components special for prodenia litura; storing at 4 ℃. The feed has the advantages of scientific formula, comprehensive components, high nutritive value, low manufacturing cost, convenient use, safety, reliability and no toxic or side effect, and the larvae fed by the feed has sufficient nutrition, rapid growth and healthy bodies, can improve the yield of prodenia litura larvae, and is a novel prodenia litura artificial feed containing traditional Chinese medicine components and a preparation method thereof.)

1. A prodenia litura artificial feed containing traditional Chinese medicine components is characterized by being prepared from the following raw materials in parts by weight: 16.7-21 parts of wheat germ, 8.37-10.50 parts of corn flour, 6.13 parts of tomato sauce, 4-4.5 parts of yeast powder, 5 parts of sucrose, 0.53-0.75 part of vitamin C, 2 parts of agar, 0.5 part of methyl p-hydroxybenzoate, 0.5 part of sorbic acid, 1-1.20 parts of vitamin complex and 80-250 parts of distilled water, and 5-10 ml of a traditional Chinese medicine alcohol extract is added.

2. The artificial feed for prodenia litura containing traditional Chinese medicine components, according to claim 1, is characterized in that the traditional Chinese medicine alcohol extract is prepared from the following traditional Chinese medicine decoction pieces in parts by weight: 8-26 parts of astragalus membranaceus, 7-20 parts of golden cypress, 8-26 parts of raw rhubarb and 8-26 parts of radix scrophulariae.

3. The prodenia litura artificial feed containing traditional Chinese medicine components according to claim 1, is characterized in that the feed is added with the following raw materials in parts by weight: 0-0.1 part of Vickers salt, 0-11 parts of casein and 0-0.05 part of choline chloride.

4. The preparation method of the prodenia litura artificial feed containing the traditional Chinese medicine components, which is characterized by comprising the following specific steps:

(1) preparing traditional Chinese medicine alcohol extract: weighing radix astragali, cortex Phellodendri, radix et rhizoma Rhei and radix scrophulariae, pulverizing into 10-30 mesh coarse powder, extracting with 60% ethanol at a ratio of 1:10 (W/V) of Chinese medicinal coarse powder to extracting agent, soaking in 60% ethanol for 15 days, filtering, collecting supernatant as Chinese medicinal ethanol extractive solution, and storing at 4 deg.C;

(2) weighing the following raw materials: wheat germ, corn flour, tomato sauce, yeast powder, sucrose, agar, casein, methylparaben, sorbic acid, multivitamins, vitamin C, wechsler's salt and choline chloride;

(3) the following raw materials are mixed: uniformly mixing wheat germ, corn flour, tomato sauce, yeast powder, agar, sucrose and casein, adding distilled water, stirring into paste, sterilizing at 121 ℃ for 30min, and cooling to 60-70 ℃ to obtain a material 1;

(4) the following raw materials are mixed: vitamin C, methyl p-hydroxybenzoate, sorbic acid, compound vitamin, Weishi salt and choline chloride are uniformly mixed with the traditional Chinese medicine alcohol extract, and distilled water is added to be stirred into paste to obtain a material 2;

(5) uniformly mixing the material 1 and the material 2, and cooling to 25 ℃ to obtain a blocky feed containing the traditional Chinese medicine components special for prodenia litura; storing at 4 ℃.

Technical Field

The invention relates to the field of artificial feeding of insects, in particular to a prodenia litura artificial feed containing traditional Chinese medicine components and a preparation method thereof.

Background

Indoor breeding of insects is an important link in pesticide activity screening, insect biological characteristic research, insect function and insect gene research at present, however, in the indoor breeding process, a large amount of insects die due to the influence of environmental conditions, nutrient elements and microorganisms. Improving the survival rate of insects becomes a key technology for indoor feeding of insects.

Prodenia litura (Spodopteralitura (Fabricius)) is a lepidoptera, and insects of the Spodoptera family are one of agricultural pests which are ubiquitous in the world, seriously harmful and difficult to control, and meanwhile, the prodenia litura is also a relatively mature insect. There are a lot of literature reports on indoor breeding technology and artificial feed composition of prodenia litura, and these artificial feeds mainly comprise natural feeds and artificial feeds, wherein the artificial feeds are widely researched and applied, and contain nutritional ingredients such as proteins, amino acids, saccharides, vitamins, inorganic substances, lipids, sterols, and the like. However, when the artificial feed is used for breeding insects indoors, the nuclear polyhedrosis virus infects spodoptera litura larvae, or the larvae generate drug resistance, so that the insects die in a large amount, or the insects die in a large amount in a short time, or the insect population declines, and the survival rate of the artificially bred spodoptera litura is low. The living space of prodenia litura larvae is filled with various microorganisms: bacteria, viruses, mycoplasma, chlamydia, fungi and the like are easy to cause various diseases under the condition of insufficient immunity of the polypide, and the survival and the body health of the polypide are seriously influenced. Generally, antibiotics are used for inhibiting bacteria and viruses which affect the growth of the prodenia litura larvae, but side effects are easily generated, and the reproductive health of the larvae is influenced most obviously.

Disclosure of Invention

The invention aims to improve the immunity of prodenia litura larvae, improve the resistance of the prodenia litura larvae to diseases, reduce the side effect of antibiotics and ensure that the raised prodenia litura larvae grow healthily.

The technical scheme of the invention is as follows: a prodenia litura artificial feed containing traditional Chinese medicine components is prepared from the following raw materials in parts by weight: 16.7-21 parts of wheat germ, 8.37-10.50 parts of corn flour, 6.13 parts of tomato sauce, 4-4.5 parts of yeast powder, 5 parts of sucrose, 0.53-0.75 part of vitamin C, 2 parts of agar, 0.5 part of methyl p-hydroxybenzoate, 0.5 part of sorbic acid, 1-1.20 parts of vitamin complex and 80-250 parts of distilled water, and 5-10 ml of a traditional Chinese medicine alcohol extract is added.

The traditional Chinese medicine alcohol extract is prepared from the following traditional Chinese medicine decoction pieces in parts by weight: 8-26 parts of astragalus membranaceus, 7-20 parts of golden cypress, 8-26 parts of raw rhubarb and 8-26 parts of radix scrophulariae.

The feed is added with the following raw materials in parts by weight: 0-0.1 part of Vickers salt, 0-11 parts of casein and 0-0.05 part of choline chloride.

A preparation method of prodenia litura artificial feed containing traditional Chinese medicine components comprises the following specific steps:

(1) preparing traditional Chinese medicine alcohol extract: weighing radix astragali, cortex Phellodendri, radix et rhizoma Rhei and radix scrophulariae, pulverizing into 10-30 mesh coarse powder, extracting with 60% ethanol at a ratio of 1:10 (W/V) to the coarse powder, soaking in 60% ethanol for 15 days, filtering, and collecting supernatant as Chinese medicinal ethanol extractive solution, and storing at 4 deg.C;

(2) weighing the following raw materials: wheat germ, corn flour, tomato sauce, yeast powder, sucrose, agar, casein, methylparaben, sorbic acid, multivitamins, vitamin C, wechsler's salt and choline chloride;

(3) the following raw materials are mixed: uniformly mixing wheat germ, corn flour, tomato sauce, yeast powder, agar, sucrose and casein, adding distilled water, stirring into paste, sterilizing at 121 ℃ for 30min, and cooling to 60-70 ℃ to obtain a material 1;

(4) the following raw materials are mixed: vitamin C, methyl p-hydroxybenzoate, sorbic acid, compound vitamin, Vickers salt and choline chloride are uniformly mixed with the traditional Chinese medicine alcohol extract, and distilled water is added to be stirred into paste to obtain a material 2;

(5) uniformly mixing the material 1 and the material 2, and cooling to 25 ℃ to obtain a blocky feed containing the traditional Chinese medicine components special for prodenia litura; storing at 4 ℃.

The spodoptera litura larvae are very small in size, 1-3 instar larvae are smaller in size, the immunity is low, and the spodoptera litura larvae are extremely easy to die due to factors such as feeding conditions and unbalanced nutrient elements in the indoor feeding process. The spodoptera litura is frequently subjected to phenomena of soft larva body, outflow of body fluid and the like in the indoor breeding process, and finally the spodoptera litura is killed in a large amount. The reason for this phenomenon has been studied mainly due to bacterial, fungal and viral infections, and to the decline of the insect population. The immunity is the ability of the insect body to resist external invasion and maintain the stability of the internal environment. When the immune system of the insect is low, the insect can not normally play a protective role, and is very easy to cause infection of bacteria, viruses, fungi and the like. The feed disclosed by the invention takes the improvement of the immunity of prodenia litura as a breakthrough, and traditional Chinese medicine components for improving the physique of the larvae, regulating the immunity of the larvae, enhancing the anti-infection capacity of the larvae, regulating the immunity of the larvae, improving the physique of the larvae and resisting the infection of germs and viruses are added into the feed. The invention takes corn flour, wheat germ and yeast powder as protein and amino acid sources. The wheat germ contains common amino acids, saccharides, fatty acids, minerals and B vitamins required by insects, but the amino acids are not balanced. Corn is one of the host plants of spodoptera litura larvae, and contains carbohydrates, proteins, fats, carotenes, riboflavin, multivitamins, and trace elements. Yeast powder is usually used as a protein source in artificial feed, and is rich in B vitamins. According to the method, the yeast powder, the corn flour and the wheat germ are reasonably matched, so that the defect of unbalanced amino acid of the wheat germ for growth of the spodoptera littoralis larvae can be overcome. Sucrose is a main source of insect energy, and the activity of sucrose enzymes in phytophagous insects is high, so that the sucrose is easy to decompose. In order to enhance the physical quality of the spodoptera litura larvae, the invention also adds vitamin C and vitamin complex into the formula. The invention can also add casein, choline chloride and Weishi salt into the formula, wherein the casein is a nutrient component for the growth of larvae and has the function of improving the survival rate of the larvae, pupae and imagoes. Choline chloride is a choline component, and can promote growth and development and improve the utilization rate of amino acid. The Weibull salt is an inorganic salt which is composed of various components and is suitable for the growth of insects, and influences the physiological and biochemical metabolism, tissue composition and growth and development of the insects. The tomato sauce is a sauce-shaped concentrated product of tomatoes, and the tomato sauce also contains B vitamins, dietary fibers, minerals, protein, natural pectin, trace elements and the like besides lycopene. The test finds that: the nutrients in tomato paste are more easily absorbed by the spodoptera litura larvae than fresh tomatoes. Lycopene in the tomato sauce has the effect of inhibiting the growth of bacteria, is an excellent antioxidant, can remove free radicals in prodenia litura larvae, and improves the physical quality of the prodenia litura larvae. The tomato sauce is sour and sweet in taste and can increase appetite, and the tomato sauce serving as a flavoring agent can increase the food intake of spodoptera litura larvae and supplement natural vitamins and trace elements in the feed, so that the feed can increase the appetite of the spodoptera litura larvae and enhance the physique of the spodoptera litura larvae. The invention takes methyl p-hydroxybenzoate and sorbic acid as main preservatives, wherein the methyl p-hydroxybenzoate is called methyl p-hydroxybenzoate, and the methyl p-hydroxybenzoate is a non-ionic surfactant, has excellent biodegradation characteristic and has the function of inhibiting bacteria and fungi in feed. The sorbic acid can effectively inhibit the activity of mould, microzyme and aerobic bacteria, thereby effectively prolonging the preservation time of the food and keeping the flavor of the original food. The methyl p-hydroxybenzoate and the sorbic acid are usually mixed and added into the artificial insect feed. In order to improve the self-resistance of prodenia litura larvae and prevent and treat bacterial, fungal and viral infection, the invention adds alcohol extract of scutellaria baicalensis, phellodendron amurense, raw rhubarb and figwort roots as the specific components on the basis of common preservatives, protein, amino acid and trace elements. Astragalus membranaceus is a labiate plant, is a commonly used qi tonifying medicine in China, has more than 2000 years of history so far, has the functions of enhancing the immunity of organisms, resisting stress and resisting wide bacteria, and can be used for symptoms such as spontaneous perspiration, night sweat due to yin deficiency, asthenia, yang-qi deficiency, lung-qi deficiency, sore ulcer and the like caused by weak body and weak surface in the medical field. Cortex Phellodendri is Rutaceae plant, and its medicinal part is cortex, and has heat clearing and detoxicating effects; heat-clearing and dampness-drying herbs mainly used for treating damp-heat dysentery and diarrhea; bone-steaming, fatigue-heat, night sweat; and sores in the mouth and tongue; conjunctival congestion with swelling and pain; carbuncle, deep-rooted carbuncle and sore; eczema of skin, etc. The radix et rhizoma Rhei is dried root and rhizome of Rheum palmatum L., Rheum tanguticum Maxim. and Rheum officinale Baill. of Polygonaceae, has effects of removing stagnation, clearing away dampness and heat, purging pathogenic fire, cooling blood, removing blood stasis, removing toxic substance, and inhibiting bacteria, and is mainly used for treating acute gastric ulcer and duodenal ulcer complicated with hemorrhage.

On the basis of mastering the biology, ecological habits and growth and development rules of spodoptera litura larvae, combining theories and methods of modern biology and breeding science, selecting traditional Chinese medicines such as scutellaria baicalensis, golden cypress, raw rhubarb and radix scrophulariae to be added into the feed according to the principle of distinguishing and treating in traditional Chinese medicine and on the basis of the understanding and treatment principle of the traditional Chinese medicine on improving resistance and strengthening the body mechanism, and organically combining and synergizing a plurality of traditional Chinese medicines, so that the feed disclosed by the invention can improve the autoimmunity and disease resistance of insects while meeting the basic nutritional requirements of the spodoptera litura. The invention is to prepare the traditional Chinese medicine into alcohol extract and then mix the alcohol extract with the feed. The alcohol extract prepared from the traditional Chinese medicine has high content of effective components, is easy to be uniformly mixed with feed, and has simple and convenient operation and low preparation cost.

The feed provided by the invention can obviously improve the immunity of spodoptera litura larvae and improve the survival rate of the larvae, particularly the survival rate of 1-3 instar larvae. The block feed can ensure that the larva feeding operation is simple, the larva bodies of the 1-3 instar larvae are very small, the artificial feed is only needed to be replaced once in the feeding process of the 1-3 instar larvae, the artificial death of the larvae caused by the replacement of the artificial feed is avoided, the operation is simplified, the labor is saved, the larvae fed by the feed are in regular growth and development, and the feed is favorable for meeting the supply of insect sources in the indoor pesticide activity screening, insect biological characteristics, biological assay, insect functions and insect gene research. The invention focuses on improving the immunity of prodenia litura, has scientific and reasonable formula, ensures that the feed has comprehensive nutrition, high nutritional value and good taste, can improve the feeding rate of prodenia litura larvae, and can improve the physical quality of the prodenia litura larvae. The 1-3 instar larvae fed by the feed have high survival rate and regular growth and development, and are favorable for meeting the supply of insect sources for indoor bioassay. The prodenia litura fed by the feed has the advantages of high survival rate of the larvae, strong bodies of the larvae, large bodies, healthy reproductive systems of the larvae, large egg laying amount, high hatching rate, balanced male and female proportion and balanced reproduction of healthy offspring. The feed has the advantages of scientific formula, comprehensive components, high nutritive value, low manufacturing cost, convenient use, safety, reliability and no toxic or side effect, and the larvae fed by the feed has sufficient nutrition, rapid growth and healthy bodies, can improve the yield of prodenia litura larvae, and is a novel prodenia litura artificial feed containing traditional Chinese medicine components and a preparation method thereof.

Detailed Description

The technical scheme and effects of the prodenia litura artificial feed containing traditional Chinese medicine components and the preparation method are further described in detail through 4 specific embodiments.

TABLE 1 alcoholic extractive solution of traditional Chinese medicine in artificial feed for prodenia litura in the formulation of examples 1-5

Raw materials	Example 1	Example 2	Example 3	Example 4	Example 5
						Radix astragali (g)	12	26	8	22	15
Cortex Phellodendri (g)	20	10	26	7	15
						Radix Et rhizoma Rhei (g)	18	8	26	22	12
Radix scrophulariae (g)	18	24	8	16	26

TABLE 2 Twill moth artificial feed formulations of examples 1-5

Raw materials	Example 1	Example 2	Example 3	Example 4	Example 5
						Wheat germ (g)	16.7	21	16.7	21	21
Corn flour (g)	8.37	10.5	8.37	10.5	10.5
						Tomato sauce (g)	6.13	6.13	6.13	6.13	6.13
Yeast powder (g)	4.5	4	4.5	4	4
						Vitamin C (g)	0.75	0.53	0.75	0.53	0.53
Agar (g)	2	2	2	2	2
						Methylparaben (g)	0.5	0.5	0.5	0.5	0.5
Sorbic acid (g)	0.5	0.5	0.5	0.5	0.5
						Compound vitamin (g)	1	1.2	1	1.2	1.2
Sucrose (g)	5	5	5	5	5
						Weishi salt (g)	0.1	0	0.1	0	0
Choline chloride (g)	0.05	0	0.05	0	0
						Casein (g)	11	0	11	0	0
Traditional Chinese medicine ethanol extract (mL)	5	5	10	15	10
						Distilled water (g)	130	100	80	250	200

The specific preparation steps of each embodiment of the preparation method of the prodenia litura artificial feed containing the traditional Chinese medicine components are as follows:

1. the method for preparing the traditional Chinese medicine alcohol extract comprises the following steps:

weighing the traditional Chinese medicines according to the table 1: crushing astragalus membranaceus, phellodendron amurense, raw rhubarb and radix scrophulariae into 10-30-mesh coarse powder, wherein an extraction reagent is 60% ethanol, the ratio of the traditional Chinese medicine coarse powder to the extraction reagent is 1:10 (W/V), soaking the traditional Chinese medicine coarse powder in the 60% ethanol for 15 days, removing residues, filtering, standing a filtrate, collecting a supernatant, namely the traditional Chinese medicine ethanol extract in the embodiment 1-5, and storing at 4 ℃;

2. preparation of prodenia litura artificial feed containing traditional Chinese medicine components

(1) Weighing the raw materials according to the table 2, uniformly mixing wheat germ, corn flour, tomato sauce, yeast powder, agar, sucrose and casein in the raw materials, adding distilled water, uniformly stirring to form paste, sterilizing at 121 ℃ for 30min, and cooling to 60-70 ℃ to obtain a material 1;

(2) mixing vitamin C, methyl p-hydroxybenzoate, sorbic acid, vitamin complex, Vickers salt and choline chloride in the raw materials with the ethanol extract of the embodiment 1-5 prepared from the traditional Chinese medicines in the table 1 uniformly, adding distilled water, and stirring into paste to obtain a material 2;

(3) uniformly mixing the material 1 and the material 2, and cooling to 25 ℃ to obtain a blocky feed containing the traditional Chinese medicine components special for prodenia litura; storing at 4 ℃.

The effect of the spodoptera litura special feed containing the traditional Chinese medicine components is tested by experiments of feeding spodoptera litura larvae with the artificial feeds prepared in examples 1-5 respectively:

(1) the feeds prepared in examples 1 to 5 were prepared into small pieces of 5g each, and day-old larvae hatched on the same day were selected;

(2) taking 5 cleaned straight tubes, placing 20 larvae in each tube, and placing 1 small piece of feed with weight of 5g prepared in examples 1-5 into 5 tubes;

(3) when the larva grows to 3 years old, the larva is changed to eat the feed once;

(4) after 4 years of age, the animals are raised singly and changed once every 2 days, and 5g of the feed is added every time.

The growth of the 1-6 th-instar spodoptera litura larvae reared using the feeds prepared according to the formulations of examples 1-5 of the present invention and the above-described methods was observed as shown in table 3.

TABLE 3 growth of feed-raised spodoptera litura larvae prepared according to the formulations of examples 1-5

The artificial feed for prodenia litura prepared as shown in table 4 is comparative example 1 and comparative example 2, the raw materials of comparative example 1 and comparative example 2 are similar in variety and different in quantity, but are within the range of the raw material variety and quantity in the claims of the present invention, and comparative example 1 and comparative example 2 are different from the present invention in that the alcohol extract of the traditional Chinese medicine of the present invention is not added, but oxytetracycline which is commonly used in feed is added.

Table 4 raw material formulations of comparative example 1 and comparative example 2

Component raw materials	Comparative example 1	Comparative example 2
			Wheat germ (g)	16.7	21
Corn flour (g)	8.37	10.5
			Tomato sauce (g)	6.13	6.13
Yeast powder (g)	4.5	4
			Vitamin C (g)	0.75	0.53
Agar (g)	2	2
			Methylparaben (g)	0.5	0.5
Sorbic acid (g)	0.5	0.5
			Compound vitamin (g)	1	1.2
Sucrose (g)	5	5
			Weishi salt (g)	0.1	0
Choline chloride (g)	0.05	0
			Casein (g)	11	0
Oxytetracycline (g)	0.25	0.25
			Distilled water (g)	130	130

The preparation methods of comparative examples 1 and 2 are substantially the same as the method of the present invention. The method comprises the following specific steps:

(1) weighing the raw materials according to the table 4, uniformly mixing wheat germ, corn flour, tomato sauce, yeast powder, agar, sucrose and casein in the raw materials, adding distilled water, uniformly stirring to form paste, sterilizing at 121 ℃ for 30min, and cooling to 60-70 ℃ to obtain a material 1;

(2) pulverizing oxytetracycline in the raw materials, uniformly mixing with vitamin C, methylparaben, sorbic acid, compound vitamin, Vickers salt and choline chloride, adding distilled water, and stirring into paste to obtain a material 2;

(3) uniformly mixing the material 1 and the material 2, and cooling to 25 ℃, thus obtaining the spodoptera litura special feed in the comparative example block shape; storing at 4 ℃.

The effect of the feed containing the traditional Chinese medicine components special for prodenia litura of the invention is checked by contrast through experiments of feeding the prodenia litura larvae with the feeds prepared in example 1, comparative example 1 and comparative example 2 respectively:

(1) the feeds prepared in example 1, comparative example 1 and comparative example 2 were prepared into 5 g-weight small pieces, and day-old larvae hatched on the same day were selected;

(2) taking 3 cleaned straight tubes, placing 20 larvae in each tube, and respectively placing 1 small piece of feed with the weight of 5g prepared in example 1, comparative example 1 and comparative example 2 in the 3 straight tubes;

(3) when the larva grows to 3 years old, the larva is changed to eat the feed once;

(4) after 4 years of age, the animals are raised singly and changed once every 2 days, and 5g of the feed is added every time.

The growth and development of 1-6 th instar spodoptera litura larvae reared by the same method using the feeds prepared according to the formulations of example 1, comparative example 1 and comparative example 2 of the present invention were observed as shown in table 5.

TABLE 5 comparison of growth of Prodenia litura larvae raised on artificial diet and comparative example

And (4) conclusion: example 1 is an artificial feed added with a traditional Chinese medicine alcohol extract, and the survival rate, pupation weight, eclosion rate, egg laying amount, hatching rate, male-female ratio and other indexes of prodenia litura larvae bred by the artificial feed are obviously higher than those of comparative example 1 and comparative example 2. The survival rate, the pupation rate and the eclosion rate of adults are high, which shows that the immunity of spodoptera litura larvae is good, most of the larvae can grow healthily and pupate and eclosion; the large weight of the pupae indicates that the larva is not only alive but also healthy and heavy; the egg laying amount and the hatching rate are high, the male-female ratio is balanced, the reproductive system of the larva is healthy, and healthy offspring can be bred in a balanced manner; the short development period indicates that the larva has sufficient nutrition and rapid growth, and can improve the yield of prodenia litura.