阅读说明：本技术 一种艾绒等级的鉴定方法 (Method for identifying moxa grade ) 是由 刘大会 彭政 杨雅雯 郭兰萍 罗丹丹 李金鑫 陈昌婕 马琳 于 2019-09-24 设计创作，主要内容包括：本公开提供了一种艾绒等级的鉴定方法,检测艾绒的氮元素含量,根据艾绒的氮元素含量判断艾绒等级,艾绒的氮元素含量越低,艾绒等级越高。本公开的鉴定方法通过随着艾绒等级的升高艾绒氮元素含量逐渐减少的特性,首次利用氮元素含量确定艾绒等级,操作方法简单有效,易于开发应用。采用本公开提供的鉴定方法鉴定艾绒等级的准确率高,其准确率可达96.23％。(The disclosure provides an identification method of moxa grade, which is characterized in that the nitrogen content of moxa is detected, the moxa grade is judged according to the nitrogen content of the moxa, and the lower the nitrogen content of the moxa is, the higher the moxa grade is. The identification method disclosed by the invention determines the moxa grade by utilizing the nitrogen element content for the first time through the characteristic that the nitrogen element content of the moxa gradually decreases along with the increase of the moxa grade, and the operation method is simple and effective and is easy to develop and apply. The method for identifying the grade of the moxa has high accuracy, and the accuracy can reach 96.23%.)

1. A method for identifying moxa grade is characterized in that the content of nitrogen elements in moxa is detected, the moxa grade is judged according to the content of the nitrogen elements in the moxa, and the lower the content of the nitrogen elements in the moxa is, the higher the moxa grade is.

2. The method for identifying moxa grade according to claim 1, wherein the relationship between the nitrogen content of moxa and the moxa grade is a linear function, a quadratic function, a cubic function, an exponential function, a power function, or a logarithmic function;

the linear function is: y is_Grade＝-19.31N+38.77，R²＝0.9136；

The quadratic function is: y is_Grade＝10.29N²-43.73N+50.46，R²＝0.9733；

The cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²＝0.9765；

The exponential function: y is_Grade＝＝63.68e^-1.374N，R²＝0.9696；

The power function: y is_Grade＝13.55N^-1.33，R²＝0.9009；

The logarithmic function: y is_Grade＝-20.04ln(N)+16.99，R²＝0.9765；

Wherein, Y_GradeIs moxa grade, and N is the content of nitrogen element.

3. The method for identifying moxa grade according to claim 2, wherein the relationship between the nitrogen content of moxa and the moxa grade is: cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²0.9765; or, Y_Grade＝＝63.68e^-1.374N，R²＝0.9696；

Preferably, the relationship between the content of nitrogen element in the moxa and the grade of the moxa is as follows: cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²＝0.9765。

4. The method for evaluating a grade of moxa according to claim 1, wherein the nitrogen element is measured by kjeldahl method.

5. The method for evaluating a grade of moxa according to claim 1, wherein the content of nitrogen element in moxa is measured by preparing moxa into a digestion solution.

6. The method for identifying grade of moxa according to claim 5, wherein the step of preparing moxa into digestion solution comprises: concentrated sulfuric acid, copper sulfate and potassium sulfate are added into moxa for heating and digestion.

7. The method for identifying the grade of moxa according to claim 5, wherein the addition ratio of moxa, concentrated sulfuric acid, copper sulfate and potassium sulfate is 0.1: 7.5-8.5: 0.7-0.9: 1.6-1.8, g: mL: mmol: mmol of the active component.

8. The method for identifying moxa grade according to claim 5, wherein the digestion by heating is carried out by: heating to 175-185 ℃ for digestion, heating to 275-285 ℃ for digestion, and heating to 415-425 ℃ for digestion.

9. The method for identifying moxa grade according to claim 8, wherein the digestion time by heating to 175-185 ℃ is 25-35 min, the digestion time by heating to 275-285 ℃ is 25-35 min, and the digestion time by heating to 415-425 ℃ is 55-65 min.

10. The method for identifying moxa grade according to claim 5, wherein the digestion means is a graphite digestion instrument.

Technical Field

The disclosure belongs to the field of quality detection of traditional Chinese medicinal materials, and particularly relates to a method for identifying moxa grade.

Background

The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.

Moxibustion, also called moxibustion therapy, is a traditional Chinese medicine therapy which takes moxa as a moxibustion material and smolders acupuncture points of a human body to adjust the physiological or pathological process of the human body so as to achieve the purpose of preventing and treating diseases. Moxibustion therapy is invented by ancient workers in China, mainly utilizes warm heat stimulation, has the effects of warming yang and tonifying qi, warming and activating meridian, removing blood stasis and dissipating stagnation, tonifying middle-jiao and replenishing qi and the like aiming at one of means of treating diseases by meridian points of a patient, and is widely applied to diseases of internal medicine, surgery, gynecology, pediatrics, ophthalmology and the like. Moxa is a velvet-like substance obtained by repeatedly drying dried leaves of Artemisia argyi Levl. et Van belonging to Compositae and screening with pestle. As the raw material of moxibustion, the quality of moxa directly influences the moxibustion curative effect. The moxa wool with low quality generates too much smoke during combustion, smells pungent, and after the moxa wool is combusted, the cone body is loose and falls off immediately when being touched, so that a human body is easily burnt. The moxa wool can not be used for recovering diseases, but can cause secondary injury to patients when receiving moxibustion therapy.

The researches of Meiquanxi and the like consider that the quality of moxa is related to the purity, and the moxa needs to be repeatedly screened in the preparation process to remove impurities such as moxa powder and the like. At the time of sale, the logo 5: 1. 10: 1. 20: 1. 30: 1, etc. series rating labels. 30: 1 refers to that 30 kg of folium artemisiae argyi is repeatedly dried in a pestle and screened to prepare 1 kg of moxa, and the moxa is called as golden moxa due to golden color. Generally, moxa having a high grade ratio is considered to have a better quality because it contains less impurities. Moxa with different grades and proportions also has different purposes. Kuang huiying considers that different grades of moxa wool have their respective clinical application fields by studying the temperature and time variation characteristics of moxa wool burning of different grades and the firmness of the moxa cone after the moxa cone is burnt out; the fine moxa (40: 1) is clinically suitable for scar moxibustion therapy, the higher-grade moxa (30: 1, 20: 1) is clinically suitable for non-scar moxibustion therapy, the lower-grade moxa (10: 1) is clinically suitable for manufacturing moxa sticks for mild moxibustion therapy, and the high-purity moxa can be used in a range of application with lower-grade purity.

The method for identifying the moxa grade in the existing market mainly aims to see the color and luster, the moxa made of aged moxa is yellow and is similar to dry loess, and the higher the moxa grade is, the less impurities are contained, and the more yellow the color is. By observing the yellow degree of the moxa color, the grade of the moxa can be simply judged. However, this method is too subjective and cannot accurately determine the actual ratio of moxa. In addition, the grade quality can be identified according to the burning condition of the moxa. The smoke emitted during burning of high-grade moxa is white, the moxa-moxibustion column formed after burning is stable, and white ash is formed in the middle after the ash is crushed. And the low-grade moxa is very big in smoke, blackened and loud in sound when burning, because impurities in the low-grade moxa burst and make sound when burning, the shape of ash after burning is irregular, and the middle ash is not white and is black. However, the combustion identification method is also empirical, so that the subjectivity is too strong, and objective identification cannot be achieved.

According to the research and study of the inventor, no quality standard corresponding to moxa grade of moxa preparation process specifications exists in the current moxa market. All moxa manufacturers also process and prepare moxa with different grades according to a method which is explored by the manufacturers, so that the grade quality of the moxa products in the current market is difficult to unify. Meanwhile, the market does not have a method for identifying and detecting the moxa grade, so that illegal merchants can carry out false publicity, the grade proportion is falsely reported to earn more profits, the market is disordered, the right of consumers is seriously damaged, and the healthy development of the moxa and moxa product market is seriously damaged.

Disclosure of Invention

In order to overcome the defects in the prior art, the present disclosure provides a method for identifying moxa grade, which can accurately and effectively detect moxa grade.

In order to achieve the purpose, the technical scheme of the disclosure is as follows:

a method for identifying moxa grade comprises detecting nitrogen content of moxa, and judging moxa grade according to the nitrogen content of moxa, wherein the lower the nitrogen content of moxa, the higher the moxa grade.

According to the application, through research on moxa in different grades, the moxa is mainly non-glandular hair tissues on the moxa sheet, and the non-glandular hair does not contain nitrogen elements. Nitrogen in folium Artemisiae Argyi is mainly present in mesophyll-related tissues. In the processing and preparation process of moxa, after the folium artemisiae argyi is crushed into moxa powder (mesophyll and other tissues) and moxa, the moxa powder is continuously screened out along with the improvement of the grade of the moxa, and the nitrogen content is gradually reduced. Through research on the nitrogen content of moxa in different production places and different grades, the nitrogen content of the moxa is gradually reduced along with the increase of the moxa grade until the moxa grade is stable and the nitrogen content is stable.

Through further research of the present disclosure, it is found that the nitrogen content and the moxa grade of moxa can be expressed by a linear function, a quadratic function, a cubic function, an exponential function, a power function, a logarithmic function, or the like.

Wherein, the relationship between the content of nitrogen element in the moxa and the grade of the moxa is as follows: y is_Grade＝-19.31N+38.77，R²＝0.9136；

Y_Grade＝10.29N²-43.73N+50.46，R²＝0.9733；Y_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²＝0.9765；Y_Grade＝＝63.68e^-1.374N，R²＝0.9696；Y_Grade＝13.55N^-1.33，R²＝0.9009；Y_Grade＝-20.04ln(N)+16.99，R²＝0.9765；

Y_GradeIs moxa grade, and N is the content of nitrogen element.

The beneficial effect of this disclosure does:

the identification method disclosed by the invention determines the moxa grade by utilizing the nitrogen element content for the first time through the characteristic that the nitrogen element content of the moxa gradually decreases along with the increase of the moxa grade, and the operation method is simple and effective and is easy to develop and apply. The method for identifying the grade of the moxa has high accuracy, and the accuracy can reach more than 96.23%.

Drawings

The accompanying drawings, which are included to provide a further understanding of the disclosure, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure and are not to limit the disclosure.

FIG. 1 is a topographical image of various moxa grades prepared in example 1 of the present disclosure;

FIG. 2 is a diagram of a linear function model established in example 2 of the present disclosure;

FIG. 3 is a diagram of a quadratic function model established in embodiment 2 of the present disclosure;

FIG. 4 is a diagram of a cubic function model established in example 2 of the present disclosure;

FIG. 5 is a diagram of an exponential function model established in example 2 of the present disclosure;

FIG. 6 is a diagram of a power function model established in embodiment 2 of the present disclosure;

fig. 7 is a diagram of a logarithmic function model established in embodiment 2 of the present disclosure.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present disclosure. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

In view of the defects of high subjectivity and difficulty in accurate identification of the existing visual color discrimination method for identifying the moxa grade, the invention provides an identification method for the moxa grade to solve the technical problems.

In a typical embodiment of the present disclosure, a method for identifying moxa grade is provided, wherein a nitrogen content of moxa is detected, and the moxa grade is determined according to the nitrogen content of moxa, and the lower the nitrogen content of moxa, the higher the moxa grade.

According to the application, through research on moxa in different grades, the moxa is mainly non-glandular hair tissues on the moxa sheet, and the non-glandular hair does not contain nitrogen elements. Nitrogen in folium Artemisiae Argyi is mainly present in mesophyll-related tissues. In the processing and preparation process of moxa, after the folium artemisiae argyi is crushed into moxa powder and moxa, the moxa powder is continuously screened out along with the improvement of the grade of the moxa, and the nitrogen content is gradually reduced. Through research on the nitrogen content of moxa in different production places and different grades, the nitrogen content of the moxa is gradually reduced along with the increase of the moxa grade until the moxa grade is stable and the nitrogen content is stable.

Through further research of the present disclosure, it is found that the nitrogen content and the moxa grade of moxa can be expressed by a linear function, a quadratic function, a cubic function, an exponential function, a power function, a logarithmic function, or the like. Thus in one or more embodiments of this embodiment, the nitrogen content of the moxa is related to the moxa grade by a linear, quadratic, cubic, exponential, power, or logarithmic function;

the linear function is: y is_Grade＝-19.31N+38.77，R²＝0.9136；

The quadratic function is: y is_Grade＝10.29N²-43.73N+50.46，R²＝0.9733；

The cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²＝0.9765；

The exponential function: y is_Grade＝＝63.68e^-1.374N，R²＝0.9696；

The power function: y is_Grade＝13.55N^-1.33，R²＝0.9009；

The logarithmic function: y is_Grade＝-20.04ln(N)+16.99，R²＝0.9765；

Wherein, Y_GradeIs moxa grade, and N is the content of nitrogen element.

In the series of embodiments, the relationship between the content of nitrogen element in moxa and the grade of moxa is as follows: cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²0.9765; or, Y_Grade＝＝63.68e^-1.374N，R²0.9696. The two functions have higher accuracy in detecting the moxa grade, and the average accuracy can reach more than 94%. Through comparative research, the relation between the nitrogen content of the moxa and the moxa grade is as follows: cubic function: y is_Grade＝-4.26N³+26.09N²-61.09N+55.84，R²0.9765, the detection model has better correlation and accuracy.

In one or more examples of this embodiment, nitrogen determination was performed on moxa using kjeldahl method.

In one or more embodiments of the present disclosure, the content of nitrogen in moxa is measured by preparing moxa into a digestion solution. The content of nitrogen element can be detected more accurately.

In the series of embodiments, the process of preparing the moxa into the digestion solution comprises the following steps: concentrated sulfuric acid, copper sulfate and potassium sulfate are added into moxa for heating and digestion. The concentrated sulfuric acid is a sulfuric acid solution with the mass fraction of more than 70%.

In the series of embodiments, the addition ratio of moxa, concentrated sulfuric acid, copper sulfate and potassium sulfate is 0.1: 7.5-8.5: 0.7-0.9: 1.6-1.8, g: mL: mmol: mmol of the active component.

In this series of examples, the process of heating digestion was: heating to 175-185 ℃ for digestion, heating to 275-285 ℃ for digestion, and heating to 415-425 ℃ for digestion.

In the series of embodiments, the digestion time is 25-35 min when the materials are heated to 175-185 ℃, 25-35 min when the materials are heated to 275-285 ℃, and 55-65 min when the materials are heated to 415-425 ℃.

In the series of embodiments, the digestion mode is graphite digestion instrument digestion.

In order to make the technical solutions of the present disclosure more clearly understood by those skilled in the art, the technical solutions of the present disclosure will be described in detail below with reference to specific examples and comparative examples.