阅读说明：本技术 一种双条杉天牛雌雄性成虫接触***配识别信息素组合物及其应用 (A kind of female male insect contact mating identification information promotor composition of semanotus bifasciatus and its application ) 是由 孔祥波 方加兴 张真 张苏芳 刘福 于 2019-09-06 设计创作，主要内容包括：本发明提供了一种双条杉天牛雌雄性成虫接触性交配识别信息素组合物及其应用,属于双条杉天牛生物防治技术领域,所述组合物包括以下重量份的组分：十一甲基二十五烷烃100份、十一甲基二十六烷烃10～15份、十一甲基二十七烷烃60～65份。所述组合物能够作为双条杉天牛雌雄性成虫接触性交配识别信息素,引诱雄性成虫、促进雄性成虫交尾行为,从而达到生态调控林间双条杉天牛的种群密度。(The present invention provides a kind of female male insect contact mating identification information promotor composition of semanotus bifasciatus and its applications, belong to semanotus bifasciatus technical field of biological control, the composition comprises the following components in parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten 10~15 parts of monomethyl hexacosane hydrocarbon, ten 60~65 parts of monomethyl heptacosane hydrocarbon.The composition can lure male insect, promote male insect mating behavior, to reach the population density of ecological regulation and control woodland semanotus bifasciatus as the female male insect contact mating identification information element of semanotus bifasciatus.)

The identification information promotor composition 1. a kind of female male insect contact of semanotus bifasciatus mates, which is characterized in that including following The component of parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten 10~15 parts of monomethyl hexacosane hydrocarbon, ten monomethyls 20 Seven 60~65 parts of alkane.

2. composition according to claim 1, which is characterized in that comprise the following components in parts by weight: ten monomethyls 20 100 parts of 5 alkane, ten 13 parts of monomethyl hexacosane hydrocarbon, ten 62 parts of monomethyl heptacosane hydrocarbon.

3. a kind of biological agent including composition of any of claims 1 or 2, which is characterized in that further include solvent；Described group Object is closed to be dissolved in solvent.

4. biological agent according to claim 3, which is characterized in that the solvent is n-hexane, pentane or ethyl alcohol.

5. biological agent according to claim 3 or 4, which is characterized in that in terms of the gross mass of the composition, the life The concentration that composition is tested in object preparation is 800~1200ng/ μ L.

6. biological agent described in composition of any of claims 1 or 2, claim 3~5 any one is in semanotus bifasciatus Application in mating behavior.

Technical field

The invention belongs to semanotus bifasciatus technical field of biological control more particularly to a kind of female male insects of semanotus bifasciatus Contact mating identification information promotor composition and its application.

Background technique

Semanotus bifasciatus (Semanotus bifasciatus) is a kind of borer pest, main harm arbor-vitae, juniper, The cupressaceae plants such as Japan cypress.Larval feeding cuts off the conveying of moisture, nutrient between skin, wood, causes needle yellow, and growing way fails, It is heavy then cause windbreak, snow break, cause training or whole tree dead when serious quickly.The infringement of semanotus bifasciatus directly affects The formation of China fir, the fast growing of cypress and high-quality good timber.There is hair on the ground such as China North China, northwest, northeast, Central China, south China, East China It is raw, it is one of 35 kinds of quarantine objects that country determines.Worm prevention and treatment is relatively difficult, just for the main control method of the worm at present It is trap-tree trapping, though effective take time and effort, be unsustainable.

The COMPLEX MIXED that the wax coat of insect cuticle is made of long chain alkane, fatty acid, alcohol, ester, aldehyde and letones Object not only acts as the effect for preventing moisture loss in insect bodies, is alternatively arranged as reproduction during female male insect finds spouse and hands over The Chemical recognition signal matched.Male Cerambycid beetles search female longicorn near host plant, only contact female in its feeler After body surface, it can just identify female and mate therewith, therefore the position for contacting sexidentifying pheromone release is exactly body wall.At present The longicorn bisexuality contact sexidentifying pheromone identified is mainly some epidermis hydrocarbon and letones, wherein most It is methyl branch alkanes or alkene, for example, the contact of Psacothea hilaris (Psacothea hilaris Pascoe) is believed Breath element is three pentadecane alkene of (Z) -21- methyl -8-；Hassock yellowish leukorrhea bee longicorn (Megacyllene caryae Gahan's) connects Touching sexidentifying pheromone ingredient is 29 carbon olefin of (Z) -9-；South American hardwood prionid (Mallodon dasystomus Say contact sexidentifying pheromone ingredient) is 2- methyl-hexacosane hydrocarbon and 2- methyl-octacosane hydrocarbon；Chest spot longicorn beetle The contact sex pheromone of (Anoplophora malasiaca) is mainly made of 3 substances, and 1) aliphatic alkane, 2) aliphatic Letones, 3) lactone material composition；The contact sexidentifying pheromone of anoplophora glabripennis be (Z) -9- tricosene hydrocarbon, (Z) -9- ppentacosene hydrocarbon, (Z) -7- ppentacosene hydrocarbon, (Z) -7- cerotene hydrocarbon and (Z) -9- cerotene hydrocarbon. Above-mentioned these use the longicorn for contacting sexidentifying pheromone, on same strain plant, the contact sexidentifying pheromone of female adult body surface It finds in mating object and mating place and plays an important role in male worm.Hair is observed by the mating behavior to a variety of longicorns Existing, usually male worm is directly contacted by feeler to identify female adult, and in clasping and during climbing up female adult back, male worm is through common touching The elytrum and pronotary at angle and labipalp contact female adult, and finally mate with bent abdomen behavior, therefore these longicorn female adults Sex pheromone be contact-type, mating behavior starts by the contact sex pheromone of female adult body surface.

Longicorn mating action process it is extremely complex, mainly include following 3 continuous steps: 1) bisexuality adult by The volatile materials of host plant release is attracted on same host over long distances；2) near host plant, male insect release Aggregation pheromone attracts bisexuality individual to assemble in short distance；3) male Cerambycid beetles swash line search in host's trunk, Once contacting female longicorn body wall, mating behavior just occurs its feeler for the two.Host's plant is related to during this serial behavior The kairomone mechanism of action of object release, between bisexuality between aggregation pheromone mechanism of action and bisexuality sex pheromone work Use mechanism.The type and ratio of male insect contact mating identification information element female for semanotus bifasciatus are unclear at present.

Summary of the invention

In view of this, the purpose of the present invention is to provide a kind of female male insect contact mating identification letters of semanotus bifasciatus Cease promotor composition and its application；The composition can lure semanotus bifasciatus male as contact mating identification information element Adult promotes male insect mating behavior, can reach the harm of Ecological Control semanotus bifasciatus if cutting off its mating behavior Purpose.

In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:

The present invention provides a kind of female male insect contact mating identification information promotor compositions of semanotus bifasciatus, including with The component of lower parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten 10~15 parts of monomethyl hexacosane hydrocarbon, ten monomethyls two 60~65 parts of heptadecane hydrocarbon.

Preferably, it comprises the following components in parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten monomethyl hexacosanes 13 parts of hydrocarbon, ten 62 parts of monomethyl heptacosane hydrocarbon.

The present invention provides a kind of biological agents including the composition, further include solvent；The composition dissolution In solvent.

Preferably, the solvent is n-hexane, pentane or ethyl alcohol.

Preferably, in terms of the gross mass of the composition, in the biological agent concentration of composition be 800~ 1200ng/μL。

Preferably, in terms of the gross mass of the composition, in the biological agent concentration of composition be 850~ 1050ng/μL。

The present invention also provides the compositions, the biological agent answering in semanotus bifasciatus biological control With.

Beneficial effects of the present invention: the present invention provides a kind of female male insect contact mating identification letters of semanotus bifasciatus Cease promotor composition and its application；The composition includes ten monomethyl pentacosane hydrocarbon, ten monomethyl hexacosane hydrocarbon and 11 Methyl heptacosane hydrocarbon；The composition can effectively lure male insect, and have stability good, and bioactivity is special One, the stability of semanotus bifasciatus woodland population can be influenced by changing its ratio.Composition provided by the invention can answer For the biological control of semanotus bifasciatus, there is good economic benefit, social benefit and Ecological Effect in woodland large-area applications Benefit.

Detailed description of the invention

Fig. 1 is the mass spectral analysis total ion chromatogram (solid phase of the female male insect body wall hydrocarbon of semanotus bifasciatus Micro-extraction, extracting head: 70 microns of CAR/DVB)；

Fig. 2 is indoor test semanotus bifasciatus male imago genitals and the bent abdomen mating behavior (centrifuge tube for being centrifuged tube model End is coated with contact mating identification information element).

Specific embodiment

The present invention provides a kind of female male insect contact mating identification information promotor compositions of semanotus bifasciatus, including with The component of lower parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten 10~15 parts of monomethyl hexacosane hydrocarbon, ten monomethyls two 60~65 parts of heptadecane hydrocarbon；It preferably comprises the following components in parts by weight: ten 100 parts of monomethyl pentacosane hydrocarbon, ten monomethyls 13 parts of hexacosane hydrocarbon, ten 62 parts of monomethyl heptacosane hydrocarbon.In the present invention, the ten monomethyls pentacosane hydrocarbon, Shi Yijia Base hexacosane hydrocarbon and ten monomethyl heptacosane hydrocarbon are all made of the commercial product of laboratory synthesis, ten monomethyl 25 Alkane 11Me-C25 (CAS:15689-71-1), ten monomethyl hexacosane hydrocarbon 11Me-C26 (CAS:68547-06-8) He Shiyi The chemical purity of methyl heptacosane hydrocarbon 11Me-C27 (CAS:15689-68-6) is preferably greater than 98%.

The present invention provides a kind of biological agents including the composition, further include solvent；The composition dissolution In solvent.In the present invention, the solvent is preferably n-hexane, pentane or ethyl alcohol.In the present invention, with the composition Gross mass meter, the concentration of composition is preferably 800~1200ng/ μ L in the biological agent, more preferably 850~ 1050ng/ μ L, most preferably 1000ng/ μ L.The present invention does not have particular/special requirement to the specification and purity of the solvent, using ability The conventional above-mentioned commercial product in domain.

The present invention also provides the composition, the biological agents in semanotus bifasciatus male and female mating behavior Using.In the present invention, ten monomethyl pentacosane hydrocarbon, ten monomethyl hexacosane hydrocarbon and ten monomethyls in the composition Mass ratio between heptacosane hydrocarbon and three mates knowledge with having contact in the body wall of semanotus bifasciatus female insect The hydrocarbon of other information function is consistent, has to male insect and tends to bioactivity；Semanotus bifasciatus can be lured male Adult, and there is male insect mating behavior stimulating activity.

Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood For limiting the scope of the present invention.