阅读说明：本技术 高通量核酸提取系统 (High-throughput nucleic acid extraction system ) 是由 刘祎 *** 曹炜 葛阳 吴炎凡 牛群 于 2019-08-29 设计创作，主要内容包括：本发明属于生物技术领域,具体涉及一种高通量核酸提取系统。本发明提供的高通量核酸提取系统,包括操作仓、机械手、磁棒、磁套、反应装置、提取装置、液体分配装置、样品扫码装置和控制装置,机械手包括至少两个可三维运动的第三轴,提取装置可活动地设置在一个第三轴上,包括用于固定磁棒的第一装载组件和用于固定磁套的第二装载组件,以分别使磁棒伸入磁套或由磁套移出及使磁套伸入反应装置或由反应装置移出；液体分配装置可活动地设置在另一个第三轴上,包括至少两个分配针,以分别在伸入反应装置或从反应装置移走的两种状态切换。机械手在控制装置作用下,运行精度高,稳定性好,同时样品扫码装置对样品自动化识别,提高了核酸提取效率。(The invention belongs to field of biotechnology, and in particular to a kind of high-throughput nucleic acid extraction system.High-throughput nucleic acid extraction system provided by the invention, including operation storehouse, manipulator, bar magnet, magnetosheath, reaction unit, extraction element, liquid dispensing apparatus, sample barcode scanning device and control device, manipulator include at least two can three-dimensional motion third axis, extraction element is movably disposed on a third axis, including the first loading assembly for fixing bar magnet and the second loading assembly for fixing magnetosheath, to make bar magnet protrude into magnetosheath respectively or be removed by magnetosheath and magnetosheath is made to protrude into reaction unit or be removed by reaction unit；Liquid dispensing apparatus is movably disposed on another third axis, including at least two distribution needles, to protrude into reaction unit or the two states removed from reaction unit switching respectively.Manipulator is under control device effect, and running precision is high, and stability is good, while sample barcode scanning device improves nucleic acid extraction efficiency to sample automatic identification.)

1. a kind of high-throughput nucleic acid extraction system, which is characterized in that including for providing enclosed environment operation storehouse and setting Manipulator, bar magnet, magnetosheath, reaction unit, extraction element, liquid dispensing apparatus, sample barcode scanning device in the operation storehouse And control device；

Wherein, the manipulator include at least two can three-dimensional motion third axis；The extraction element includes movable respectively The first loading assembly on one third axis for fixing the bar magnet and second for fixing the magnetosheath is set Loading assembly, to make the bar magnet protrude into the magnetosheath respectively or be removed by the magnetosheath and to protrude into the magnetosheath described anti- It answers in device or by being removed in the reaction unit；The liquid dispensing apparatus includes movable respectively is arranged described in another At least two distribution needles on third axis, to protrude into remove in the reaction unit or out of described reaction unit two respectively Kind state switches over；The sample barcode scanning device includes barcode scanning mechanism and automation auxiliary body, is known with automating to sample Not；The control device is connected with the manipulator.

2. high-throughput nucleic acid extraction system according to claim 1, which is characterized in that first loading assembly includes the One N-shaped frame and at least one first fixed link being detachably arranged in the first N-shaped frame；The second loading assembly packet At least one second fixed link for including the second N-shaped frame and being detachably arranged in the second N-shaped frame.

3. high-throughput nucleic acid extraction system according to claim 2, which is characterized in that the first loading assembly setting exists The top of second loading assembly, and connect with the third axis by sliding rail.

4. high-throughput nucleic acid extraction system according to claim 3, which is characterized in that the liquid dispensing apparatus further includes Driving mechanism and the regulating mechanism being arranged in the driving mechanism, the regulating mechanism include at least two rank gears and divide The tooth plate not engaged with gear described in every rank, the distribution needle are arranged on the tooth plate.

5. high-throughput nucleic acid extraction system according to claim 4, which is characterized in that the regulating mechanism includes three ranks point Tooth plate described in the circular diameter gear incremented by successively and corresponding three rank is spent, gear described in three ranks is same by the driving mechanism Axis driving, tooth plate described in three ranks respectively under the driving of the gear described in every rank dislocation motion to adjust the spacing of the distribution needle.

6. high-throughput nucleic acid extraction system according to claim 5, which is characterized in that the reaction unit includes array hole Road mechanism, the automation auxiliary body include it is several for drive array duct mechanism rotation deck, for pair The limit hole and tracks that the array duct mechanism is limited, the barcode scanning mechanism along the tracks move with Array duct automatic mechanism identification to rotation.

7. high-throughput nucleic acid extraction system according to claim 6, which is characterized in that the liquid dispensing apparatus further includes The injection pump machanism being connected with the distribution needle.

8. high-throughput nucleic acid extraction system according to claim 7, which is characterized in that the manipulator includes being arranged in parallel Two first axles, movable the second axis of two be arranged in the first axle and it is movable be arranged each described second Two third axis on axis.

9. -8 any high-throughput nucleic acid extraction system according to claim 1, which is characterized in that further include being arranged described Heating oscillating mechanism, the drop being connected in operation storehouse and with the control device capture mechanism, clearing and drying device and disinfection Sterilizing unit.

10. high-throughput nucleic acid extraction system according to claim 9, which is characterized in that the clearing and drying device includes Wiper mechanism and drier.

Technical field

The invention belongs to field of biotechnology, and in particular to a kind of high-throughput nucleic acid extraction system.

Background technique

Nucleic acid is a kind of important large biological molecule and modern biochemistry and molecule of storage and transmitting life-information The core element of biological study.With the high speed development of molecular biology in recent years, emerge large quantities of using nucleic acid as base The molecular biology experiment of plinth, such as the sequencing of two generations, quantitative fluorescent PCR, genetic chip, making nucleic acid molecular hybridization, and DNA's is complete Whole property, the palliating degradation degree of RNA and the purity of nucleic acid and concentration etc. will all generate molecular biology experiment result more or less Influence, therefore molecular biology experiment problems faced first is how fast and efficiently to extract from complex samples The higher nucleic acid of purity.

Current existing multiple nucleic acids extracting method, such as phenol-chloroform extraction process, alkali density method, cetyltrimethylammonium Amine (CTAB) extraction process, pellosil absorption method and Beads enrichment method etc., and it has been developed based on the nucleic acid extraction of the above method Instrument.However, with the development of biological industry, the trend that the sample of nucleic acid extraction increases in quantity and complexity is deepened, in order to suitable This trend is answered, developing high-throughput instrument for extracting nucleic acid becomes current hot spot.Wherein, the nucleic acid separation based on Beads enrichment method Instrument extracts process according to automation can be divided into drawing-in type and magnetic bar type again, and the two main distinction is: the core based on drawing-in type Sour extraction apparatus is the extraction purification that nucleic acid is realized by transfer reaction solution, and step includes: that lysate is added into sample, Mixing is blown and beaten, magnetic bead adsorbs, removes lysate, cleaning solution washing magnetic bead is added, adsorbs to magnetic bead, removes cleaning solution, add repeatedly Entering the operations such as eluent can be obtained the nucleic acid of purifying, but during transfer liquid, and bottom has liquid residual, so as to cause Salinity residual is big, nucleic acid purity is relatively low, and if sample is too sticky, for drawing the automation liquid relief system of liquid Easily there is blocked situation in system；And the instrument for extracting nucleic acid based on bar magnet method includes bar magnet, magnetosheath and deep-well plates etc., step Include: sample is added in deep-well plates and is cracked when carrying out nucleic acid extraction, while in system containing magnetic bead with will be in lysate Nucleic acid absorption to magnetic bead surfaces, hereafter wash miscellaneous process by bar magnet transfer with nucleic acid magnetic bead it is complete in different deep-well plates At the nucleic acid salinity residual that this scheme is extracted is low, and purity is higher, and is not in blockage problem, but traditional base In bar magnet method instrument for extracting nucleic acid the problem is that: 1) nucleic acid eluents after purification need transfer by hand, in transfer process It is easy to appear manual operation fault, causes the pollution of human factor, generates false positive；2) it washes, wash, elute through supersalt, step It is more, time-consuming, in particular within a short period of time handle great amount of samples when, cross contamination is serious, and error rate greatly improves；3) In the multiple transfer process of magnetic bead, the nucleic acid being adsorbed on magnetic bead is exposed to outside, is easy by the impurity in environment such as dust, enzyme Deng pollution, especially RNA is easily degraded；4) equipment Market price is higher, maintenance cost is high, each functional module harmony, Flexibility is poor.

Summary of the invention

Therefore, the technical problem to be solved in the present invention is that overcoming the existing instrument for extracting nucleic acid based on bar magnet method because automatic Change degree is not high enough, each functional module harmony and flexibility it is poor it is cumbersome so as to cause extraction step, error rate is high, there are samples The defect of this pollution etc., thus provide a kind of each functional module flexibility and integration more preferably, extraction step is simple, error rate is low High-throughput nucleic acid extraction system.

In order to solve the above technical problems, the technical solution adopted by the present invention is that:

The present invention provides a kind of high-throughput nucleic acid extraction system, including for providing enclosed environment operation storehouse and setting Manipulator, bar magnet, magnetosheath, reaction unit, extraction element, liquid dispensing apparatus, sample barcode scanning device in the operation storehouse And control device；

Wherein, the manipulator include at least two can three-dimensional motion third axis；The extraction element includes respectively can It is movably arranged on the third axis for fixing the first loading assembly of the bar magnet and for fixing the magnetosheath Second loading assembly, to make the bar magnet protrude into the magnetosheath respectively or be removed by the magnetosheath and the magnetosheath is made to protrude into institute It states in reaction unit or by being removed in the reaction unit；The liquid dispensing apparatus includes movable respectively is arranged at another At least two distribution needles on the third axis, to protrude into the reaction unit or removed out of described reaction unit respectively Two states switch over；The sample barcode scanning device includes barcode scanning mechanism and automation auxiliary body, with automatic to sample Change identification；The control device is connected with the manipulator.

Preferably, the high-throughput nucleic acid extraction system, first loading assembly include the first N-shaped frame and detachably set Set at least one first fixed link in the first N-shaped frame；Second loading assembly includes the second N-shaped frame and removable Unload at least one second fixed link being arranged in the second N-shaped frame.

It is further preferred that the high-throughput nucleic acid extraction system, the first loading assembly setting is loaded described second The top of component, and connect with the third axis by sliding rail.

It is further preferred that the high-throughput nucleic acid extraction system, the liquid dispensing apparatus further include driving mechanism and Regulating mechanism in the driving mechanism is set, and the regulating mechanism is including at least two rank gears and respectively and described in every rank The tooth plate of gear engagement, the distribution needle are arranged on the tooth plate.

It is further preferred that the high-throughput nucleic acid extraction system, the regulating mechanism include three rank reference diameters successively Tooth plate described in the incremental gear and corresponding three rank, gear described in three ranks is by the driving mechanism Driven by Coaxial, three ranks The tooth plate respectively the gear described in every rank driving under dislocation motion with adjust it is described distribution needle spacing.

It is further preferred that the high-throughput nucleic acid extraction system, the reaction unit includes array duct mechanism, it is described from Dong Hua auxiliary body includes several is used for drive the deck of the array duct mechanism rotation, for array duct machine The limit hole and tracks that structure is limited, the barcode scanning mechanism are moved along the tracks with the battle array to rotation Arrange the identification of duct automatic mechanism.

It is further preferred that the high-throughput nucleic acid extraction system, the liquid dispensing apparatus further include and the distribution needle The injection pump machanism being connected.

It is further preferred that the high-throughput nucleic acid extraction system, the manipulator include two first axles disposed in parallel, Movable the second axis of two be arranged in the first axle and movable two institutes being arranged on each second axis State third axis.

Preferably, the high-throughput nucleic acid extraction system, further include be arranged in the operation storehouse and with the control device Heating oscillating mechanism, the drop being connected capture mechanism, clearing and drying device and disinfecting device.

It is further preferred that the high-throughput nucleic acid extraction system, the clearing and drying device include wiper mechanism and drying Mechanism.

Technical solution of the present invention has the advantages that

1. high-throughput nucleic acid extraction system provided by the invention, including operation storehouse and the machinery being arranged in the operation storehouse Hand, bar magnet, magnetosheath, reaction unit, extraction element, liquid dispensing apparatus, sample barcode scanning device and control device.

The high-throughput core extraction system of the structure, manipulator include at least two can three-dimensional motion third axis, wherein Movably be provided with extraction element on one third axis, the extraction element include for fix the first loading assembly of bar magnet and For fixing the second loading assembly of magnetosheath, to make bar magnet protrude into magnetosheath respectively or be removed by magnetosheath and magnetosheath is made to protrude into reaction In device or by being removed in reaction unit；It is movably provided with liquid dispensing apparatus on another third axis, the liquid point With device include at least two distribution needles, with respectively the two states for protruding into reaction unit or being removed out of reaction unit into Row switching.Manipulator can be accurately realized relative motion and each distribution between bar magnet and magnetosheath under control device effect The single pass pipetting of needle, running precision are high, and stability is good, while sample barcode scanning device is to sample automatic identification, and record Enter sample message, improves the efficiency of nucleic acid extraction.

2. high-throughput nucleic acid extraction system provided by the invention, the first loading assembly include the first N-shaped frame and with this The first fixed link that one N-shaped frame is detachably connected, the second loading assembly include the second N-shaped frame and removable with the second N-shaped frame Unload the second fixed link of connection, if the loader mechanism property of can choose of the structure load with nucleic acid extraction experiment flux it is comparable Dry set bar magnet and magnetosheath are tested, and the three-dimensional motion of achievable bar magnet and magnetosheath is mentioned under the drive of third axis with carrying out nucleic acid Take process.

3. high-throughput nucleic acid extraction system provided by the invention, regulating mechanism includes that three rank reference diameters are incremented by successively Gear and with every rank gear accordingly three rank tooth plate, three rank gears are by driving mechanism Driven by Coaxial, then three rank tooth plates exist respectively Every rank gear driving is lower to generate dislocation motion, so that relative motion occurs for the multiple distribution needles being arranged on every rank tooth plate, that is, Spacing between multiple distribution needles can realize accurate adjusting, and flexibility is good, and freedom degree is high, can match the reaction dress of different spacing It sets.

4. high-throughput nucleic acid extraction system provided by the invention further includes that setting is connected in operation storehouse and with control device Heating oscillating mechanism, the drop connect captures mechanism, clearing and drying device and disinfecting device, and it is automatable to lead to control device It realizes the building that high-throughput nucleic acid extracts, compared with existing similar product, there is better flexibility and higher integration.

Detailed description of the invention

It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor It puts, is also possible to obtain other drawings based on these drawings.

Fig. 1 is the high-throughput nucleic acid extraction system structural schematic diagram that the embodiment of the present invention 1 provides；

Fig. 2 is Fig. 1 schematic diagram of internal structure；

Fig. 3 is base vertical view in Fig. 1；

Fig. 4 is the high-throughput nucleic acid extraction system control device electric control principle schematic diagram that the embodiment of the present invention 1 provides；

Fig. 5 is heating oscillating mechanism and droplet capture mechanism structure schematic diagram；

Fig. 6 is that oscillation component explosive view is heated in Fig. 1；

Fig. 7 is that bar magnet, magnetosheath hang status diagram on hanging rack in Fig. 1；

Fig. 8 is bar magnet, magnetosheath the stationary state schematic diagram on loader mechanism in Fig. 1；

Fig. 9 is liquid dispensing apparatus schematic diagram in Fig. 1；

Figure 10 is regulating mechanism schematic diagram in Fig. 9；

Figure 11 is Tu1Zhong barcode scanning mechanism structural schematic diagram；

Figure 12 is barcode scanning mechanism schematic diagram of internal structure in Figure 11；

Figure 13 is clearing and drying device structural schematic diagram in Fig. 1；

Figure 14 is clearing and drying device schematic diagram of internal structure in Figure 13；

Description of symbols:

1- operates storehouse；11- pedestal；12- cabinet；121- window；13- windowing；131- form；

2- control device；21- power switch；22- scram button；23-PC terminal；

3- manipulator；31- first axle；The second axis of 32-；33- third axis；

4- reaction unit；41- array duct mechanism；42- heats oscillating mechanism；421- first shell；422- heating oscillation Component；The first pedestal of 4221-；The first groove of 42211-；4222- electromagnet；4223- vibrating mass；4224- cover board；4225- is heat-insulated Pad；4226- heating plate；4227- heat-conducting plate；4228- position-limited rack；

5- extraction element；51- bar magnet；52- magnetosheath；53- hanging rack；54- loader mechanism；The first loading assembly of 541-； 5411- the first N-shaped frame；The first fixed link of 5412-；The second loading assembly of 542-；5421- the second N-shaped frame；5422- second is fixed Bar；55- droplet capture mechanism；The recessed disk of 551-；552- support leg；56- cryo-conservation mechanism；

6- liquid dispensing apparatus；61- second shell；611- fixed strip；62- driving mechanism；63- regulating mechanism；631- tooth Wheel assembly；632- tooth plate component；64- distributes needle；65- injects pump machanism；

7- sample barcode scanning device；71- barcode scanning mechanism；72- automates auxiliary body；721- third shell；722- servo electricity Machine；723- belt wheel；724- deck；725- limit hole；726- tracks；

8- clearing and drying device；The 4th shell of 81-；82- wiper mechanism；82- drier；84- sewage pipe；

9- disinfecting device.

Specific embodiment

Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical", The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second ", " third " is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition Concrete meaning in invention.

As long as in addition, the non-structure each other of technical characteristic involved in invention described below different embodiments It can be combined with each other at conflict.