阅读说明：本技术 一种铜绿丽金龟的引诱剂 (Attractant for Aerugo chafer ) 是由 杨霞 朱佳敏 赵玉雪 吴柳燕 刘靖 付品 张亚洲 于 2021-08-19 设计创作，主要内容包括：本发明公开了一种铜绿丽金龟的引诱剂,属于昆虫防治技术领域,该引诱剂为核壳结构,包括外壳层和内核层；所述内核层被分成两部分,两部分被隔开,第一部分包括：甲基丁香酚、β-紫罗兰醇、香草醇、红糖、香叶烯、乙醇、穿心莲内酯、橙花醇、苯乙酸卞酯、顺-3-己烯戊醇以及4-甲基伞酮-β-D半乳糖苷,第二部分为β-半乳糖苷酶；所述外壳层为明胶,本发明引诱剂具有很强的诱捕铜绿丽金龟能力,稳定性好,专一性强,且不会使铜绿丽金龟产生抗性。(The invention discloses an attractant for Aerugo scarab, belonging to the technical field of insect control, wherein the attractant is of a core-shell structure and comprises an outer shell layer and an inner core layer; the inner core layer is divided into two portions, the two portions being spaced apart, the first portion comprising: methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate, cis-3-hexenyl alcohol and 4-methylumbelliferone-beta-D galactoside, and the second part is beta-galactosidase; the shell layer is made of gelatin, and the attractant has strong capability of trapping the verdigris, good stability and strong specificity, and can not cause the verdigris to generate resistance.)

1. An attractant for Aerugo chafer is characterized in that the attractant is of a core-shell structure and comprises an outer shell layer and an inner core layer; the inner core layer is divided into two portions, the two portions being spaced apart, the first portion comprising: methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate, cis-3-hexenyl alcohol and 4-methylumbelliferone-beta-D galactoside, and the second part is beta-galactosidase; the outer shell layer is gelatin.

2. An attractant as claimed in claim 1, wherein the first part of the inner core layer comprises the following components in parts by weight: 1.8-4.5 parts of methyl eugenol, 3.1-5.8 parts of beta-ionol, 1.5-3.5 parts of vanillyl alcohol, 1-5 parts of brown sugar, 10-15 parts of myrcene, 40-60 parts of ethanol, 0.5-3.7 parts of andrographolide, 2.6-4.8 parts of nerol, 1-5 parts of benzyl phenylacetate, 1-5 parts of cis-3-hexenyl alcohol and 2.5-16 parts of 4-methylumbelliferone-beta-D galactoside, and the second part is 2-15 parts of beta-galactosidase.

3. An attractant as claimed in claim 1 wherein said outer coating is 35-45 parts by weight gelatin.

4. A method for preparing the attractant of any one of claims 1 to 3, wherein the method comprises the following steps:

(1) weighing the raw materials of the first part of the inner core layer according to the parts by weight, uniformly mixing the raw materials, adding gelatin for granulation, and drying;

(2) coating the first and second parts of the inner core layer with gelatin to prepare an outer shell layer, granulating, and drying.

Technical Field

The invention relates to the technical field of insect prevention and treatment, in particular to an attractant for a mossback aeruginosa.

Background

The underground pests are recognized at home and abroad as major pests which are difficult to predict and control and mainly damage openings of crop seedlings, roots, tubers and the like. Grubs are the general term for larvae of the superfamily carapace of coleoptera, are the largest group of underground pests, and are the species causing the greatest loss and the greatest harm. The larvae and adults of the tortoise shell can cause serious harm, and the larvae mainly feed underground rhizomes and pods of various crops to cause withering and death of the crops; and the imagoes eat leaves of crops, fruit trees, forest trees and the like, so that bud residue leaves are broken, even bare branches and bald stalks are generated, and the quality and the yield of agricultural products are seriously influenced. According to survey statistics, 86% of plant underground part damage is caused by the larva of the tortoise plastron, and the existing chemical agents are the main means for preventing and controlling the tortoise plastron, wherein the prevention and the control of the larva are the main means. The adult is prone to occur in the daytime and at night, and the adaptability is strong. The emergence time of the imagoes is concentrated, especially the imagoes are in explosive damage after rain, if the imagoes are not prevented and controlled timely, leaves of fruit trees and forest trees are easy to be notched and holes are easy to be caused in a short time, in case of serious imagoes, leaf veins and leaf stalks are only remained, even bare branches and bald stems are used, and the imagoes are more serious in damage to newly planted fruit tree seedlings such as apples, walnuts, cherries and the like.

There are more than 10 species of tortoise shells harmful to crops, and the species vary from place to place, and the species mainly include two species of the limnanthes cuckoo and the gill-chafer. The Anomala corpulenta, Holotrichia oblita and H.parallella holotricha are dominant populations harmful to peanuts, particularly the biggest incidence and the most serious harm of the Anomala corpulenta. In recent years, due to global climate warming, agricultural planting systems (no-tillage shallow-tillage technology, crop rotation system, single-seed sowing, mulching planting and the like), increased ecological environment control strength (environmental greening, returning to farming and the like) and the like, hidden breeding places and favorable feeding and inhabitation places are provided for the white grubs, the insect source accumulation is large, and the damage of the white grubs is increased year by year.

In the past, chemical agents with long efficacy duration are mainly used for preventing and treating, most of the insecticides applied in the prior art are pesticides with short residual life duration, and ideal effects are difficult to obtain by adopting traditional pesticide application methods such as soil treatment, medicament seed dressing and the like to prevent and treat grub hazards of autumn crops. With the limitation and the prohibition of some high-toxicity and high-residue pesticides (organic phosphorus, carbamates and the like), the control effect of many current medicaments on the grubs is not ideal. Meanwhile, the soil is also polluted permanently or semi-permanently to different degrees, which leads to the problem of quality safety of agricultural products. Therefore, the investigation of behavior regulation measures such as trapping and killing of the soil insect imagoes becomes one of the important means for controlling the soil insect imagoes and pesticide residues.

Disclosure of Invention

The invention aims to provide the attractant special for the Aerugo chafer, which improves the attraction effect on the Aerugo chafer.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides an attractant for a mossback aeruginosa, which is of a core-shell structure and comprises an outer shell layer and an inner core layer; the inner core layer is divided into two portions, the two portions being spaced apart, the first portion comprising: methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate, cis-3-hexenyl alcohol and 4-methylumbelliferone-beta-D galactoside, and the second part is beta-galactosidase; the outer shell layer is gelatin.

Further, the inner core layer comprises the following components in parts by weight: 1.8-4.5 parts of methyl eugenol, 3.1-5.8 parts of beta-ionol, 1.5-3.5 parts of vanillyl alcohol, 1-5 parts of brown sugar, 10-15 parts of myrcene, 40-60 parts of ethanol, 0.5-3.7 parts of andrographolide, 2.6-4.8 parts of nerol, 1-5 parts of benzyl phenylacetate, 1-5 parts of cis-3-hexenyl alcohol and 2.5-16 parts of 4-methylumbelliferone-beta-D galactoside, and the second part is 2-15 parts of beta-galactosidase.

Further, the outer shell layer is 35-45 parts by weight of gelatin.

The invention also provides a preparation method of the attractant, which comprises the following steps:

(1) weighing the raw materials of the first part of the inner core layer according to the parts by weight, uniformly mixing the raw materials, adding gelatin for granulation, and drying, wherein the gelatin can be granulated, most importantly, the outer surface of the first part is coated with a gelatin layer, and when the first part and the second part of the inner core layer are coated with the gelatin in the step (2), the first part and the second part are separated and do not react;

(2) coating the first and second parts of the inner core layer with gelatin to prepare an outer shell layer, granulating, and drying.

The attractant disclosed by the invention is of a core-shell structure and comprises an inner core layer and an outer shell layer, wherein the inner core layer comprises a first part and a second part, the first part is coated by gelatin to ensure that the first part and the second part are separated, and then the inner core layer is coated by transparent gelatin to form the outer shell layer, so that the first part and the second part of the inner core layer are prevented from reacting in the storage and transportation processes, and the carrying is convenient. When the attractant is used, the attractant is mixed with the bacillus, the gelatin can be degraded due to the bacillus, the 4-methylumbelliferone-beta-D galactoside of the first part is contacted with the beta-galactosidase of the second part, and then the gelatin is decomposed into the high-intensity fluorescent substance 4-methylumbelliferone under the action of the beta-galactosidase to emit fluorescence, the wavelength of the excitation light is 360nm, the wavelength of the emission light is 450nm, and the spectrum can induce the patina tortoises. In addition, methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate and cis-3-hexenyl pentanol can also volatilize out to attract the verdigris.

The invention discloses the following technical effects:

the invention is added with methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate, cis-3-hexenyl pentanol, 4-methyl umbelliferone-beta-D galactoside and beta-galactosidase in a compounding way, when in use, the attractant is mixed with the bacillus, the gelatin is degraded, the chafer aeruginosa can be induced by the unique smell, after the first part of the 4-methylumbelliferone-beta-D galactoside is contacted with the second part of the beta-galactosidase, the beta-galactosidase decomposes the beta-galactosidase into a high-strength fluorescent substance 4-methyl umbelliferone which emits fluorescence, has strong capability of trapping the Aerugo tortoise, good stability and strong specificity, and can not cause the Aerugo tortoise to generate resistance.

Detailed Description

Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.

It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.

In the examples of the present invention, each material, component and bacterium was purchased from commercial sources.

The granulation method of the present invention is a conventional technical means, and is not the focus of the present invention, and is not described herein again.

Examples

The luring test of the Aerugo scarab is carried out in Guiyang city of Guizhou province from 6 months 1 to 6 months 30 days in 2021, the raw materials are mixed uniformly according to a proportion before the luring test and then are put into a transparent plastic bottle with the same specification and then are fixed in a trapper, a test group designs 6 groups of formulas (examples 1 to 6, see table 1 in detail) and a comparison group 1 (LED lamp with the wavelength of 400 nm), a comparison group 2 (sugar-vinegar, white sugar, vinegar, white spirit and water in a mass ratio of 3:4:1:2) and a comparison group 3 (only the attractant of example 1 is added, and no Bacillus thuringiensis is added), the attractants of examples 1 to 6 are mixed with the Bacillus thuringiensis (examples 1 to 5 of the Bacillus thuringiensis account for 10% of the total mass of the attractants, and example 6 of the Bacillus thuringiensis accounts for 5% of the total mass of the attractants), the lures are put into the transparent plastic bottle, 3 trappers are repeatedly arranged in each test group and the comparison group and are randomly distributed, the height from the ground of each trap is 1.5-2m, the interval distance is 80-90m, and the number and the types of insects trapped by each trap are counted and recorded every 10 days.

The attractants of examples 1-6 are core-shell structures, including an outer shell layer and an inner core layer; the inner core layer is divided into two portions, the two portions being spaced apart, the first portion comprising: methyl eugenol, beta-ionol, vanillyl alcohol, brown sugar, myrcene, ethanol, andrographolide, nerol, benzyl phenylacetate, cis-3-hexenyl alcohol and 4-methylumbelliferone-beta-D galactoside, and the second part is beta-galactosidase; the outer shell layer is gelatin.

The attractants of examples 1-6 were prepared as follows:

(1) uniformly mixing the raw materials, adding gelatin, granulating, and drying to coat a gelatin layer on the outer surface of the first part;

(2) coating the first and second parts of the inner core layer with gelatin to prepare an outer shell layer, granulating, and drying.

The specific composition of the attractants of examples 1-6 is shown in Table 1.

TABLE 1

The results of the trap tests for each group are shown in Table 2.

TABLE 2

As shown in Table 2, when the amount of Bacillus used was small, the trapping speed was slow, the cycle was long, and when the amount was large, the trapping speed was fast, and the cycle was short. The attractant can specifically attract the Aerugo chafer and has poor trapping effect on other coleoptera insects.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.