阅读说明：本技术 樟芝菌丝体活性物质用于改善慢性阻塞性肺病的用途 (Antrodia camphorata mycelium active material is used to improve the purposes of chronic obstructive pulmonary disease ) 是由 陈劲初 李宜蓁 林定威 张晓苓 陈彦博 于 2018-07-12 设计创作，主要内容包括：本发明提供一种樟芝菌丝体活性物质用于制备改善慢性阻塞性肺病的组合物的用途。所述樟芝菌丝体活性物质是以下列步骤制备而成：(a)取一樟芝菌丝体(Antrodia cinnamomea)于平板培养基上,于15-30℃的温度下培养4-10天；(b)将步骤(a)培养后的樟芝菌丝体接种至一烧瓶内,于15-30℃、pH 2-6的环境培养3-14天；(c)将步骤(b)培养后的樟芝菌丝体接种于一发酵槽内,于15-30℃、pH2-6的环境下搅拌培养3-21天,形成含有该樟芝菌丝体活性物质的樟芝菌丝体发酵液。(The present invention provides a kind of Antrodia camphorata mycelium active material and is used to prepare the purposes for improving the composition of chronic obstructive pulmonary disease.The Antrodia camphorata mycelium active material is prepared with the following steps: (a) being taken an Antrodia camphorata mycelium (Antrodia cinnamomea) on plating medium, is cultivated 4-10 days at a temperature of 15-30 DEG C；(b) Antrodia camphorata mycelium after step (a) culture is seeded in a flask, in 15-30 DEG C, environment culture 3-14 days of pH 2-6；(c) Antrodia camphorata mycelium after step (b) culture is inoculated in a fermentation tank, stir culture 3-21 days in the environment of 15-30 DEG C, pH2-6 forms the Antrodia camphorata mycelium fermented liquid containing the Antrodia camphorata mycelium active material.)

1. a kind of Antrodia camphorata mycelium active material is used to prepare the purposes for improving the composition of chronic obstructive pulmonary disease, wherein should Antrodia camphorata mycelium active material is prepared with the following steps:

(a) it takes an Antrodia camphorata mycelium on plating medium, is cultivated 4-10 days at a temperature of 15-30 DEG C；

(b) Antrodia camphorata mycelium after step (a) culture is seeded in a flask, in 15-30 DEG C, the environment culture 3- of pH 2-6 14 days；

(c) Antrodia camphorata mycelium after step (b) culture is inoculated in a fermentation tank, is stirred in the environment of 15-30 DEG C, pH2-6 Culture 3-21 days is mixed, the Antrodia camphorata mycelium fermented liquid for containing the Antrodia camphorata mycelium active material is formed.

2. purposes as described in claim 1, wherein the step of preparing the Antrodia camphorata mycelium active material further includes step (d): will be milled after the Antrodia camphorata mycelium fermented liquid freeze-drying, form the antrodia for containing the Antrodia camphorata mycelium active material Mycelium freeze-dried powder.

3. purposes as claimed in claim 2, wherein the step of preparing the Antrodia camphorata mycelium active material further includes step (e): by the Antrodia camphorata mycelium freeze-dried powder with an at least solvent extraction, being formed containing the Antrodia camphorata mycelium active material extremely A few Antrodia camphorata mycelium extract liquor.

4. purposes as claimed in claim 3, wherein the step of preparing the Antrodia camphorata mycelium active material further includes step (f): the Antrodia camphorata mycelium extract liquor is dry, to obtain the Antrodia camphorata mycelium active material.

5. purposes as described in claim 3 or 4, wherein in step (e), the solvent is selected from water or alcohols.

6. purposes as claimed in claim 5, wherein when the solvent of step (e) is water, the Antrodia camphorata mycelium activity The effective concentration of substance is between 0.5-1mg/ml.

7. purposes as claimed in claim 5, wherein when the solvent of step (e) is alcohols, the Antrodia camphorata mycelium is living The effective concentration of property substance is between 50-100 μ g/ml.

8. purposes as described in claim 1, wherein the flask culture of step (b) is shake culture, and revolving speed is 110- 130rpm。

9. purposes as described in claim 1, wherein fermentation tank described in step (c) is further passed through a gas, the gas packet Air, oxygen, carbon dioxide, helium or combinations thereof are included, the slot pressure of the fermentation tank is 0.5-1.0kg/cm²And Ventilation Rate is 0.01-1.5VVM。

10. purposes as described in claim 1, wherein the composition is medical composition, and the medical composition is further Include pharmaceutically acceptable carrier, excipient, diluent or adjuvant.

11. purposes as described in claim 1, wherein the composition is food additives.

Technical field

A kind of preparation method and its usage of the present invention about Antrodia camphorata mycelium active material.Specifically, about preparation A kind of Antrodia camphorata mycelium active material and its purposes in food or pharmaceuticals for improving chronic obstructive pulmonary disease.

Background technique

Chronic Obstructive Pulmonary Disease (Chronic Obstructive Pulmonary Disease, abbreviation COPD) is current The 4th of the global cause of death is occupied, is a kind of common, multiple, high disability rate and high lethality rate chronic respiratory disease.Entirely The patient of world's chronic obstructive pulmonary disease has more than 300,000,000 people.

Smoking is the main reason for leading to COPD, so in recent years because of locomotive, the waste air of automobile and industrial power generation and combustion Oily coal-fired use increases, and causes the air pollution of Particulate Matter (PM) 2.5 to rise, more aggravates this disease Occur.It is chronically exposed in the environment of pollution, lung can be made to generate inflammatory response, airflow channel is caused to narrow and generate lung tissue Dysfunction.

The therapeutic modality of COPD includes smoking cessation at present, lung rehabilitation, imbedibility bronchodilators, solid using cortex class Alcohol, long-term oxygen supply or progress lung transplantation etc..2015 it is newest by new drug be to merge two kinds of muscle for being used in COPD in the past Relaxant does not have breakthrough new drug development yet so far.Therefore, research effectively treats the method for chronic obstructive pulmonary disease still It is important developing direction.

Antrodia (Antrodia cinnamomea), also known as camphor tree mushroom, camphor tree wild rice, mushroom in camphor tree, cinnamomum kanahirai hay mushroom, Antrodia camphorata, red camphor tree, Red antrodia, the rotten heartwood inner wall of the cinnamomum kanehirai dry rot being only grown between height above sea level 450-1500 meters of TaiWan, China mountain area or it is withered fall The dark moist surface of the Cinnamomum kanahirai hay timber of volt, belongs to the precious medicinal fungi of TaiWan, China national treasure grade.Still lack antrodia pair at present Improve the research of chronic obstructive pulmonary disease.

Summary of the invention

The present invention provides a kind of Antrodia camphorata mycelium active material and preparation method thereof, and can be used for preparing tool improves chronic resistance The composition of plug property tuberculosis.Compared to general Western medicine and treatment method, the invention discloses liquid state fermentation Antrodia camphorata mycelium activity The preparation method of substance is safer, easy, and manufactured Antrodia camphorata mycelium active material is more natural, safe, and can be effectively improved COPD。

An embodiment according to the present invention provides a kind of Antrodia camphorata mycelium active material and is used to prepare improvement chronic obstructive The purposes of the composition of tuberculosis, wherein Antrodia camphorata mycelium active material is prepared with the following steps:

(a) Antrodia camphorata mycelium (Antrodia cinnamomea) is taken on plating medium, at a temperature of 15-30 DEG C Culture 4-10 days；

(b) Antrodia camphorata mycelium after step (a) culture is seeded in flask, in 15-30 DEG C, the environment culture of pH 2-6 3-14 days；

(c) Antrodia camphorata mycelium after step (b) culture is inoculated in fermentation tank, in the environment of 15-30 DEG C, pH2-6 Stir culture 3-21 days, form the Antrodia camphorata mycelium fermented liquid containing Antrodia camphorata mycelium active material.

In one embodiment, the step of preparing Antrodia camphorata mycelium active material further includes step (d): will be Antrodia camphorata mycelium fermented It is milled after liquid freeze-drying, forms the Antrodia camphorata mycelium freeze-dried powder containing Antrodia camphorata mycelium active material.

In one embodiment, the step of preparing Antrodia camphorata mycelium active material further includes step (e): Antrodia camphorata mycelium is lyophilized Powder forms the Antrodia camphorata mycelium extract liquor containing Antrodia camphorata mycelium active material with an at least solvent extraction.

In one embodiment, the step of preparing Antrodia camphorata mycelium active material further includes step (f): Antrodia camphorata mycelium is extracted Liquid is dry, to obtain Antrodia camphorata mycelium active material.

In one embodiment, the solvent in step (e) is selected from water or alcohols.

In one embodiment, when the solvent of step (e) is water, the effective concentration of Antrodia camphorata mycelium active material between 0.25-1mg/ml。

In one embodiment, when the solvent of step (e) is alcohols, the effective concentration of Antrodia camphorata mycelium active material between 25-100μg/ml。

In one embodiment, the flask culture of above-mentioned steps (b) is shake culture, and revolving speed is 110-130rpm.

In one embodiment, the fermentation tank in step (c) is further passed through a gas, this gas includes air, oxygen, dioxy Change carbon, helium or combinations thereof, the slot pressure of fermentation tank is 0.5-1.0kg/cm²And Ventilation Rate is 0.01-1.5VVM.

In one embodiment, the composition is medical composition, this medical composition further includes pharmaceutically acceptable Carrier, excipient, diluent or adjuvant.

In one embodiment, the composition is food additives.

Detailed description of the invention

Antrodia camphorata mycelium water extraction object, which is shown, in Fig. 1 improves cigarette smoke extract induction alveolar epithelium A549 cell damage Ability as a result, wherein AcH (L), AcH (M) and AcH (H) respectively refer to Antrodia camphorata mycelium water extraction object concentration be 0.25mg/ml, 0.5mg/ml and 1.0mg/ml.

Antrodia camphorata mycelium alcohol extraction object, which is shown, in Fig. 2 improves cigarette smoke extract induction alveolar epithelium A549 cell damage Ability as a result, wherein AcMe (L), AcMe (M) and AcMe (H) respectively refer to Antrodia camphorata mycelium alcohol extraction object concentration be 25 μ g/ml, 50 μ g/ml and 100 μ g/ml.

Specific embodiment

To keep above and other aspect of the invention apparent understandable, special embodiment below, and cooperate institute's accompanying drawings It is described in detail.